Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate
Само за регистроване кориснике
2018
Аутори
Mitić, NevenaStanisić, Mariana
Savić, Jelena
Cosić, Tatjana
Stanisavljević, Nemanja
Miljuš-Đukić, Jovanka
Marin, Marija
Radović, Svetlana
Ninković, Slavica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataa seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes ...were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.
Кључне речи:
Wheat / Morphophysiological changes / Hairy root exudate / Cytological changes / Chenopodium murale / AllelopathyИзвор:
Protoplasma, 2018, 255, 6, 1683-1692Издавач:
- Springer Wien, Wien
Финансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
- Молекуларни механизми одговора биљака на абиотички стрес-улога транскрипционих фактора и малих РНК и анализа генетичког диверзитета биљних култура од интереса за пољопривреду и биотехнологију (RS-MESTD-Basic Research (BR or ON)-173005)
- Бело или/и мрко: значај масног ткива у одржању укупне редокс зависне метаболичке контроле у физиолошким адаптацијама и метаболичким поремећајима (RS-MESTD-Basic Research (BR or ON)-173055)
DOI: 10.1007/s00709-018-1250-0
ISSN: 0033-183X
PubMed: 29748859
WoS: 000447405900009
Scopus: 2-s2.0-85046754190
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mitić, Nevena AU - Stanisić, Mariana AU - Savić, Jelena AU - Cosić, Tatjana AU - Stanisavljević, Nemanja AU - Miljuš-Đukić, Jovanka AU - Marin, Marija AU - Radović, Svetlana AU - Ninković, Slavica PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1139 AB - Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataa seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes. PB - Springer Wien, Wien T2 - Protoplasma T1 - Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate EP - 1692 IS - 6 SP - 1683 VL - 255 DO - 10.1007/s00709-018-1250-0 ER -
@article{ author = "Mitić, Nevena and Stanisić, Mariana and Savić, Jelena and Cosić, Tatjana and Stanisavljević, Nemanja and Miljuš-Đukić, Jovanka and Marin, Marija and Radović, Svetlana and Ninković, Slavica", year = "2018", abstract = "Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataa seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.", publisher = "Springer Wien, Wien", journal = "Protoplasma", title = "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate", pages = "1692-1683", number = "6", volume = "255", doi = "10.1007/s00709-018-1250-0" }
Mitić, N., Stanisić, M., Savić, J., Cosić, T., Stanisavljević, N., Miljuš-Đukić, J., Marin, M., Radović, S.,& Ninković, S.. (2018). Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate. in Protoplasma Springer Wien, Wien., 255(6), 1683-1692. https://doi.org/10.1007/s00709-018-1250-0
Mitić N, Stanisić M, Savić J, Cosić T, Stanisavljević N, Miljuš-Đukić J, Marin M, Radović S, Ninković S. Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate. in Protoplasma. 2018;255(6):1683-1692. doi:10.1007/s00709-018-1250-0 .
Mitić, Nevena, Stanisić, Mariana, Savić, Jelena, Cosić, Tatjana, Stanisavljević, Nemanja, Miljuš-Đukić, Jovanka, Marin, Marija, Radović, Svetlana, Ninković, Slavica, "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate" in Protoplasma, 255, no. 6 (2018):1683-1692, https://doi.org/10.1007/s00709-018-1250-0 . .