Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats
Аутори
Stanisavljević, Suzana![](/themes/Mirageimagine/images/orcid.png)
Cepić, Aleksa
Bojić, Svetlana
Veljović, Katarina
![](/themes/Mirageimagine/images/orcid.png)
Mihajlović, Sanja
Dedović, Neda
![](/themes/Mirageimagine/images/orcid.png)
Jevtić, Bojan
![](/themes/Mirageimagine/images/orcid.png)
Momcilović, Miljana
Lazarević, Milica
Mostarica-Stojković, Marija
Miljković, Đorđe
![](/themes/Mirageimagine/images/orcid.png)
Golić, Nataša
![](/themes/Mirageimagine/images/orcid.png)
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Gut microbiota dysbiosis has been considered the essential element in the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). Antibiotics were administered orally to Dark Agouti (DA) rats early in their life with the aim of perturbing gut microbiota and investigating the effects of such intervention on the course of EAE. As a result, the diversity of the gut microbiota was reduced under the influence of antibiotics. Mainly, Firmicutes and Actinobacteria were replaced by Proteobacteria and Bacteroidetes, while decreased proportions of Clostridia and Bacilli classes were accompanied by an increase in Gamma-Proteobacteria in antibiotic-treated animals. Interestingly, a notable decrease in the Helicobacteraceae, Spirochaetaceae and Turicibacteriaceae was scored in antibiotic-treated groups. Also, levels of short chain fatty acids were reduced in the faeces of antibiotic-treated rats. Consequently, aggravation of EAE, paralleled with str...onger immune response in lymph nodes draining the site of immunization, and increased inflammation within the CNS, were observed in antibiotic-treated DA rats. Thus, the alteration of gut microbiota leads to an escalation of CNS-directed autoimmunity in DA rats. The results of this study indicate that antibiotic use in early life may have subsequent unfavourable effects on the regulation of the immune system.
Извор:
Scientific Reports, 2019, 9, 918-Издавач:
- Nature Publishing Group, London
Финансирање / пројекти:
- Изучавање гена и молекуларних механизама у основи пробиотичке активности бактерија млечне киселине изолованих са подручја западног Балкана (RS-MESTD-Basic Research (BR or ON)-173019)
- Ћелијски и молекулски механизми опоравка пацова од експерименталног аутоимунског енцефаломијелитиса (RS-MESTD-Basic Research (BR or ON)-173035)
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.1038/s41598-018-37505-7
ISSN: 2045-2322
PubMed: 30696913
WoS: 000456956200008
Scopus: 2-s2.0-85060813602
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stanisavljević, Suzana AU - Cepić, Aleksa AU - Bojić, Svetlana AU - Veljović, Katarina AU - Mihajlović, Sanja AU - Dedović, Neda AU - Jevtić, Bojan AU - Momcilović, Miljana AU - Lazarević, Milica AU - Mostarica-Stojković, Marija AU - Miljković, Đorđe AU - Golić, Nataša PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1272 AB - Gut microbiota dysbiosis has been considered the essential element in the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). Antibiotics were administered orally to Dark Agouti (DA) rats early in their life with the aim of perturbing gut microbiota and investigating the effects of such intervention on the course of EAE. As a result, the diversity of the gut microbiota was reduced under the influence of antibiotics. Mainly, Firmicutes and Actinobacteria were replaced by Proteobacteria and Bacteroidetes, while decreased proportions of Clostridia and Bacilli classes were accompanied by an increase in Gamma-Proteobacteria in antibiotic-treated animals. Interestingly, a notable decrease in the Helicobacteraceae, Spirochaetaceae and Turicibacteriaceae was scored in antibiotic-treated groups. Also, levels of short chain fatty acids were reduced in the faeces of antibiotic-treated rats. Consequently, aggravation of EAE, paralleled with stronger immune response in lymph nodes draining the site of immunization, and increased inflammation within the CNS, were observed in antibiotic-treated DA rats. Thus, the alteration of gut microbiota leads to an escalation of CNS-directed autoimmunity in DA rats. The results of this study indicate that antibiotic use in early life may have subsequent unfavourable effects on the regulation of the immune system. PB - Nature Publishing Group, London T2 - Scientific Reports T1 - Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats SP - 918 VL - 9 DO - 10.1038/s41598-018-37505-7 ER -
@article{ author = "Stanisavljević, Suzana and Cepić, Aleksa and Bojić, Svetlana and Veljović, Katarina and Mihajlović, Sanja and Dedović, Neda and Jevtić, Bojan and Momcilović, Miljana and Lazarević, Milica and Mostarica-Stojković, Marija and Miljković, Đorđe and Golić, Nataša", year = "2019", abstract = "Gut microbiota dysbiosis has been considered the essential element in the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). Antibiotics were administered orally to Dark Agouti (DA) rats early in their life with the aim of perturbing gut microbiota and investigating the effects of such intervention on the course of EAE. As a result, the diversity of the gut microbiota was reduced under the influence of antibiotics. Mainly, Firmicutes and Actinobacteria were replaced by Proteobacteria and Bacteroidetes, while decreased proportions of Clostridia and Bacilli classes were accompanied by an increase in Gamma-Proteobacteria in antibiotic-treated animals. Interestingly, a notable decrease in the Helicobacteraceae, Spirochaetaceae and Turicibacteriaceae was scored in antibiotic-treated groups. Also, levels of short chain fatty acids were reduced in the faeces of antibiotic-treated rats. Consequently, aggravation of EAE, paralleled with stronger immune response in lymph nodes draining the site of immunization, and increased inflammation within the CNS, were observed in antibiotic-treated DA rats. Thus, the alteration of gut microbiota leads to an escalation of CNS-directed autoimmunity in DA rats. The results of this study indicate that antibiotic use in early life may have subsequent unfavourable effects on the regulation of the immune system.", publisher = "Nature Publishing Group, London", journal = "Scientific Reports", title = "Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats", pages = "918", volume = "9", doi = "10.1038/s41598-018-37505-7" }
Stanisavljević, S., Cepić, A., Bojić, S., Veljović, K., Mihajlović, S., Dedović, N., Jevtić, B., Momcilović, M., Lazarević, M., Mostarica-Stojković, M., Miljković, Đ.,& Golić, N.. (2019). Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats. in Scientific Reports Nature Publishing Group, London., 9, 918. https://doi.org/10.1038/s41598-018-37505-7
Stanisavljević S, Cepić A, Bojić S, Veljović K, Mihajlović S, Dedović N, Jevtić B, Momcilović M, Lazarević M, Mostarica-Stojković M, Miljković Đ, Golić N. Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats. in Scientific Reports. 2019;9:918. doi:10.1038/s41598-018-37505-7 .
Stanisavljević, Suzana, Cepić, Aleksa, Bojić, Svetlana, Veljović, Katarina, Mihajlović, Sanja, Dedović, Neda, Jevtić, Bojan, Momcilović, Miljana, Lazarević, Milica, Mostarica-Stojković, Marija, Miljković, Đorđe, Golić, Nataša, "Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats" in Scientific Reports, 9 (2019):918, https://doi.org/10.1038/s41598-018-37505-7 . .