Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii
Apstrakt
Although the molecular mechanisms of carbapenem resistance of environmental isolates ofAcinetobacter baumanniiare well described, data on the mechanisms of colistin resistance are scarce. In this study, we report the molecular mechanisms of colistin resistance in environmental isolates ofA. baumannii. Seven clinically relevant isolates ofA. baumanniibelonging to ST-2(Pasteur)were recovered from hospital wastewater and wastewater treatment plant. The phenotypic resistance to colistin was confirmed by broth microdilution with minimum inhibitory concentration values ranging from 20 to 160 mg/L. Colistin sulfate and colistimethate sodium showed bactericidal activity against two colistin-heteroresistant isolatesin vitro, but substantially recovery of population was observed after prolonged incubation.In silicogenome analysis revealed nucleotide variations resulting in amino acid changes in LpxC (N286D), LpxD (E117K), PmrB (A138T, R263S, L267W, Q309P, and A444V), and EptA (F166L, I228V, R348...K, A370S, and K531T). According to reverse transcription quantitative PCR, all isolates had increased levels ofeptAmRNA and decreased levels oflpxAandlpxDmRNA. Isolates expressed low hydrophobicity, biofilm, and pellicle formation, but showed excellent survival in river water during 50 days of monitoring. Colistin- and pandrug-resistantA. baumanniidisseminated in the environment could represent the source for the occurrence of serious community-acquired infections.
Ključne reči:
wastewater / resistance / environment / colistin / Acinetobacter baumanniiIzvor:
Microbial Drug Resistance, 2021, 27, 3, 328-336Izdavač:
- Mary Ann Liebert, Inc, New Rochelle
Finansiranje / projekti:
- Ministry of Education, Science and Technological Development of the Republic of Serbia
- Croatian Science Foundation [IP-2014-09-5656]
DOI: 10.1089/mdr.2020.0188
ISSN: 1076-6294
PubMed: 32762604
WoS: 000558377300001
Scopus: 2-s2.0-85102930921
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jovčić, Branko AU - Novović, Katarina AU - Dekić, Svjetlana AU - Hrenović, Jasna PY - 2021 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1485 AB - Although the molecular mechanisms of carbapenem resistance of environmental isolates ofAcinetobacter baumanniiare well described, data on the mechanisms of colistin resistance are scarce. In this study, we report the molecular mechanisms of colistin resistance in environmental isolates ofA. baumannii. Seven clinically relevant isolates ofA. baumanniibelonging to ST-2(Pasteur)were recovered from hospital wastewater and wastewater treatment plant. The phenotypic resistance to colistin was confirmed by broth microdilution with minimum inhibitory concentration values ranging from 20 to 160 mg/L. Colistin sulfate and colistimethate sodium showed bactericidal activity against two colistin-heteroresistant isolatesin vitro, but substantially recovery of population was observed after prolonged incubation.In silicogenome analysis revealed nucleotide variations resulting in amino acid changes in LpxC (N286D), LpxD (E117K), PmrB (A138T, R263S, L267W, Q309P, and A444V), and EptA (F166L, I228V, R348K, A370S, and K531T). According to reverse transcription quantitative PCR, all isolates had increased levels ofeptAmRNA and decreased levels oflpxAandlpxDmRNA. Isolates expressed low hydrophobicity, biofilm, and pellicle formation, but showed excellent survival in river water during 50 days of monitoring. Colistin- and pandrug-resistantA. baumanniidisseminated in the environment could represent the source for the occurrence of serious community-acquired infections. PB - Mary Ann Liebert, Inc, New Rochelle T2 - Microbial Drug Resistance T1 - Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii EP - 336 IS - 3 SP - 328 VL - 27 DO - 10.1089/mdr.2020.0188 ER -
@article{ author = "Jovčić, Branko and Novović, Katarina and Dekić, Svjetlana and Hrenović, Jasna", year = "2021", abstract = "Although the molecular mechanisms of carbapenem resistance of environmental isolates ofAcinetobacter baumanniiare well described, data on the mechanisms of colistin resistance are scarce. In this study, we report the molecular mechanisms of colistin resistance in environmental isolates ofA. baumannii. Seven clinically relevant isolates ofA. baumanniibelonging to ST-2(Pasteur)were recovered from hospital wastewater and wastewater treatment plant. The phenotypic resistance to colistin was confirmed by broth microdilution with minimum inhibitory concentration values ranging from 20 to 160 mg/L. Colistin sulfate and colistimethate sodium showed bactericidal activity against two colistin-heteroresistant isolatesin vitro, but substantially recovery of population was observed after prolonged incubation.In silicogenome analysis revealed nucleotide variations resulting in amino acid changes in LpxC (N286D), LpxD (E117K), PmrB (A138T, R263S, L267W, Q309P, and A444V), and EptA (F166L, I228V, R348K, A370S, and K531T). According to reverse transcription quantitative PCR, all isolates had increased levels ofeptAmRNA and decreased levels oflpxAandlpxDmRNA. Isolates expressed low hydrophobicity, biofilm, and pellicle formation, but showed excellent survival in river water during 50 days of monitoring. Colistin- and pandrug-resistantA. baumanniidisseminated in the environment could represent the source for the occurrence of serious community-acquired infections.", publisher = "Mary Ann Liebert, Inc, New Rochelle", journal = "Microbial Drug Resistance", title = "Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii", pages = "336-328", number = "3", volume = "27", doi = "10.1089/mdr.2020.0188" }
Jovčić, B., Novović, K., Dekić, S.,& Hrenović, J.. (2021). Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii. in Microbial Drug Resistance Mary Ann Liebert, Inc, New Rochelle., 27(3), 328-336. https://doi.org/10.1089/mdr.2020.0188
Jovčić B, Novović K, Dekić S, Hrenović J. Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii. in Microbial Drug Resistance. 2021;27(3):328-336. doi:10.1089/mdr.2020.0188 .
Jovčić, Branko, Novović, Katarina, Dekić, Svjetlana, Hrenović, Jasna, "Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii" in Microbial Drug Resistance, 27, no. 3 (2021):328-336, https://doi.org/10.1089/mdr.2020.0188 . .