Regulation of rpoS gene expression in Pseudomonas: involvement of a TetR family regulator
Апстракт
The rpoS gene encodes the sigma factor which was identified in several gram-negative bacteria as a central regulator during stationary phase. rpoS gene regulation is known to respond to cell density, showing higher expression in stationary phase. For Pseudomonas aeruginosa, it has been demonstrated that the cell-density dependent regulation response known as quorum sensing interacts with this regulatory response. Using the rpoS promoter of P. putida, we identified a genomic Tn5 insertion mutant of P. putida which showed a 90% decrease in rpoS promoter activity, resulting in less RpoS being present in a cell at stationary phase. Molecular analysis revealed that this mutant carried a Tn5 insertion in a gene, designated psrA (Pseudomonas sigma regulator), which codes for a protein (PsrA) of 26.3 kDa. PsrA contains a helix-turn-helix motif typical of DNA binding proteins and belongs to the TetR family of bacterial regulators. The homolog of the psrA gene was identified in P. aeruginosa; th...e protein showed 90% identity to PsrA of P. putida. A psrA::Tn5 insertion mutant of P, aeruginosa was constructed, In both Pseudomonas species, psrA was genetically linked to the SOS lexA repressor gene. Similar to what was observed for P. putida, a psrA null mutant of P. aeruginosa also showed a 90% reduction in rpoS promoter activity; both mutants could be complemented for rpoS promoter activity when the psrA gene was provided in trans, psrA mutants of both Pseudomonas species lost the ability to induce rpoS expression at stationary phase, but they retained the ability to produce quorum-sensing autoinducer molecules. PsrA was demonstrated to negatively regulate psrA gene expression in Pseudomonas and in Escherichia coli as well as to be capable of activating the rpoS promoter in E. call. Our data suggest that PsrA is an important regulatory protein of Pseudomonas spp. involved in the regulatory cascade controlling rpoS gene regulation in response to tell density.
Извор:
Journal of Bacteriology, 2001, 183, 12, 3712-3720Издавач:
- Amer Soc Microbiology, Washington
DOI: 10.1128/JB.183.12.3712-3720.2001
ISSN: 0021-9193
PubMed: 11371535
WoS: 000168990400020
Scopus: 2-s2.0-0034991107
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Milan AU - Venturi, V PY - 2001 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/157 AB - The rpoS gene encodes the sigma factor which was identified in several gram-negative bacteria as a central regulator during stationary phase. rpoS gene regulation is known to respond to cell density, showing higher expression in stationary phase. For Pseudomonas aeruginosa, it has been demonstrated that the cell-density dependent regulation response known as quorum sensing interacts with this regulatory response. Using the rpoS promoter of P. putida, we identified a genomic Tn5 insertion mutant of P. putida which showed a 90% decrease in rpoS promoter activity, resulting in less RpoS being present in a cell at stationary phase. Molecular analysis revealed that this mutant carried a Tn5 insertion in a gene, designated psrA (Pseudomonas sigma regulator), which codes for a protein (PsrA) of 26.3 kDa. PsrA contains a helix-turn-helix motif typical of DNA binding proteins and belongs to the TetR family of bacterial regulators. The homolog of the psrA gene was identified in P. aeruginosa; the protein showed 90% identity to PsrA of P. putida. A psrA::Tn5 insertion mutant of P, aeruginosa was constructed, In both Pseudomonas species, psrA was genetically linked to the SOS lexA repressor gene. Similar to what was observed for P. putida, a psrA null mutant of P. aeruginosa also showed a 90% reduction in rpoS promoter activity; both mutants could be complemented for rpoS promoter activity when the psrA gene was provided in trans, psrA mutants of both Pseudomonas species lost the ability to induce rpoS expression at stationary phase, but they retained the ability to produce quorum-sensing autoinducer molecules. PsrA was demonstrated to negatively regulate psrA gene expression in Pseudomonas and in Escherichia coli as well as to be capable of activating the rpoS promoter in E. call. Our data suggest that PsrA is an important regulatory protein of Pseudomonas spp. involved in the regulatory cascade controlling rpoS gene regulation in response to tell density. PB - Amer Soc Microbiology, Washington T2 - Journal of Bacteriology T1 - Regulation of rpoS gene expression in Pseudomonas: involvement of a TetR family regulator EP - 3720 IS - 12 SP - 3712 VL - 183 DO - 10.1128/JB.183.12.3712-3720.2001 ER -
@article{ author = "Kojić, Milan and Venturi, V", year = "2001", abstract = "The rpoS gene encodes the sigma factor which was identified in several gram-negative bacteria as a central regulator during stationary phase. rpoS gene regulation is known to respond to cell density, showing higher expression in stationary phase. For Pseudomonas aeruginosa, it has been demonstrated that the cell-density dependent regulation response known as quorum sensing interacts with this regulatory response. Using the rpoS promoter of P. putida, we identified a genomic Tn5 insertion mutant of P. putida which showed a 90% decrease in rpoS promoter activity, resulting in less RpoS being present in a cell at stationary phase. Molecular analysis revealed that this mutant carried a Tn5 insertion in a gene, designated psrA (Pseudomonas sigma regulator), which codes for a protein (PsrA) of 26.3 kDa. PsrA contains a helix-turn-helix motif typical of DNA binding proteins and belongs to the TetR family of bacterial regulators. The homolog of the psrA gene was identified in P. aeruginosa; the protein showed 90% identity to PsrA of P. putida. A psrA::Tn5 insertion mutant of P, aeruginosa was constructed, In both Pseudomonas species, psrA was genetically linked to the SOS lexA repressor gene. Similar to what was observed for P. putida, a psrA null mutant of P. aeruginosa also showed a 90% reduction in rpoS promoter activity; both mutants could be complemented for rpoS promoter activity when the psrA gene was provided in trans, psrA mutants of both Pseudomonas species lost the ability to induce rpoS expression at stationary phase, but they retained the ability to produce quorum-sensing autoinducer molecules. PsrA was demonstrated to negatively regulate psrA gene expression in Pseudomonas and in Escherichia coli as well as to be capable of activating the rpoS promoter in E. call. Our data suggest that PsrA is an important regulatory protein of Pseudomonas spp. involved in the regulatory cascade controlling rpoS gene regulation in response to tell density.", publisher = "Amer Soc Microbiology, Washington", journal = "Journal of Bacteriology", title = "Regulation of rpoS gene expression in Pseudomonas: involvement of a TetR family regulator", pages = "3720-3712", number = "12", volume = "183", doi = "10.1128/JB.183.12.3712-3720.2001" }
Kojić, M.,& Venturi, V.. (2001). Regulation of rpoS gene expression in Pseudomonas: involvement of a TetR family regulator. in Journal of Bacteriology Amer Soc Microbiology, Washington., 183(12), 3712-3720. https://doi.org/10.1128/JB.183.12.3712-3720.2001
Kojić M, Venturi V. Regulation of rpoS gene expression in Pseudomonas: involvement of a TetR family regulator. in Journal of Bacteriology. 2001;183(12):3712-3720. doi:10.1128/JB.183.12.3712-3720.2001 .
Kojić, Milan, Venturi, V, "Regulation of rpoS gene expression in Pseudomonas: involvement of a TetR family regulator" in Journal of Bacteriology, 183, no. 12 (2001):3712-3720, https://doi.org/10.1128/JB.183.12.3712-3720.2001 . .