TetR family member PsrA directly binds the Pseudomonas rpoS and psrA promoters
Апстракт
We have previously described a Pseudomonas gene, psrA, which enhances transcription of the rpoS sigma factor gene at stationary phase. We present molecular data which demonstrate that in Pseudomonas putida PsrA binds specifically to the rpoS and psrA promoters in DNA regions having similar palindromic sequences, C/GAAAC N2-4 GTTTG/C, where N is any nucleotide. The position of the initiation of transcription was determined for both promoters, and PsrA binds from positions - 59 to -35 in the rpoS promoter and from - 18 to + 20 in the psrA promoter with respect to the +1 transcription site. Expression studies with a psrA-lacZ transcriptional fusion in wild-type and psrA::Tn5 knockout mutants revealed that psrA was under additional control in response to growth phase. A model for the role of PsrA in the regulation of rpoS and psrA is presented.
Извор:
Journal of Bacteriology, 2002, 184, 8, 2324-2330Издавач:
- Amer Soc Microbiology, Washington
DOI: 10.1128/JB.184.8.2324-2330.2002
ISSN: 0021-9193
PubMed: 11914368
WoS: 000174669100034
Scopus: 2-s2.0-0036206512
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Milan AU - Aguilar, C AU - Venturi, V PY - 2002 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/168 AB - We have previously described a Pseudomonas gene, psrA, which enhances transcription of the rpoS sigma factor gene at stationary phase. We present molecular data which demonstrate that in Pseudomonas putida PsrA binds specifically to the rpoS and psrA promoters in DNA regions having similar palindromic sequences, C/GAAAC N2-4 GTTTG/C, where N is any nucleotide. The position of the initiation of transcription was determined for both promoters, and PsrA binds from positions - 59 to -35 in the rpoS promoter and from - 18 to + 20 in the psrA promoter with respect to the +1 transcription site. Expression studies with a psrA-lacZ transcriptional fusion in wild-type and psrA::Tn5 knockout mutants revealed that psrA was under additional control in response to growth phase. A model for the role of PsrA in the regulation of rpoS and psrA is presented. PB - Amer Soc Microbiology, Washington T2 - Journal of Bacteriology T1 - TetR family member PsrA directly binds the Pseudomonas rpoS and psrA promoters EP - 2330 IS - 8 SP - 2324 VL - 184 DO - 10.1128/JB.184.8.2324-2330.2002 ER -
@article{ author = "Kojić, Milan and Aguilar, C and Venturi, V", year = "2002", abstract = "We have previously described a Pseudomonas gene, psrA, which enhances transcription of the rpoS sigma factor gene at stationary phase. We present molecular data which demonstrate that in Pseudomonas putida PsrA binds specifically to the rpoS and psrA promoters in DNA regions having similar palindromic sequences, C/GAAAC N2-4 GTTTG/C, where N is any nucleotide. The position of the initiation of transcription was determined for both promoters, and PsrA binds from positions - 59 to -35 in the rpoS promoter and from - 18 to + 20 in the psrA promoter with respect to the +1 transcription site. Expression studies with a psrA-lacZ transcriptional fusion in wild-type and psrA::Tn5 knockout mutants revealed that psrA was under additional control in response to growth phase. A model for the role of PsrA in the regulation of rpoS and psrA is presented.", publisher = "Amer Soc Microbiology, Washington", journal = "Journal of Bacteriology", title = "TetR family member PsrA directly binds the Pseudomonas rpoS and psrA promoters", pages = "2330-2324", number = "8", volume = "184", doi = "10.1128/JB.184.8.2324-2330.2002" }
Kojić, M., Aguilar, C.,& Venturi, V.. (2002). TetR family member PsrA directly binds the Pseudomonas rpoS and psrA promoters. in Journal of Bacteriology Amer Soc Microbiology, Washington., 184(8), 2324-2330. https://doi.org/10.1128/JB.184.8.2324-2330.2002
Kojić M, Aguilar C, Venturi V. TetR family member PsrA directly binds the Pseudomonas rpoS and psrA promoters. in Journal of Bacteriology. 2002;184(8):2324-2330. doi:10.1128/JB.184.8.2324-2330.2002 .
Kojić, Milan, Aguilar, C, Venturi, V, "TetR family member PsrA directly binds the Pseudomonas rpoS and psrA promoters" in Journal of Bacteriology, 184, no. 8 (2002):2324-2330, https://doi.org/10.1128/JB.184.8.2324-2330.2002 . .