Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle
Authors
Milovanović, MinaBošković, Srđan
Jasnić, Jovana
Novković, Mirjana
Milošević, Emilija
Kojić, Snežana
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In our previous work, using transgenic zebrafish line TgBAC(ankrd1a:EGFP), we showed activation
of the zebrafish ankrd1a gene in border zone cardiomyocytes of cryoinjured heart and in close
proximity of needle-stab wounds in skeletal muscle, indicating its involvement in muscle
regeneration. Our results implicated ankrd1a in zebrafish skeletal muscle tissue repair and
remodeling, as a sensor of stressed muscle. Here we take a closer look at the spatio-temporal
expression profile of the ankrd1a gene in injured zebrafish skeletal muscle by analyzing cryosections
prepared from wounded tissue of TgBAC(ankrd1a:EGFP) adults at 1, 3, 5, 7 and 10 days post-injury
(dpi). The expression of the fluorescent reporter was observed from 3 dpi and remained until 10 dpi.
At 3dpi, new GFP-positive muscle cells emerged inside the injury zone, at the site of needle entry,
while in the later days (5, 7 and 10 dpi), newly formed GFP-positive myofibers were visible in the
deeper tissue layers withi...n the injury, indicating active repair of the injured tissue. To identify cells
in which ankrd1a is activated after injury, we stained the sections for markers of satellite-like cells,
undifferentiated and differentiated muscle cells, and mature myofibers. Since the reporter was
detected both in the newly formed myofibers that invade the wound and in the apparently uninjured
tissue surrounding the injury, we hypothesize that ankrd1a is not only involved in satellite celldependent tissue repair, but its expression might be a hallmark of adaptive process in undamaged
myofibers surrounding the physical injury.
Keywords:
ankrd1a / skeletal muscle / repair / zebrafishSource:
12th European Zebrafish Meeting, 2023, 14, 276-276Note:
- EZM2023 Abstract Book: 12th European Zebrafish Meeting July 9-13, 2023 Krakow, Poland
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Milovanović, Mina AU - Bošković, Srđan AU - Jasnić, Jovana AU - Novković, Mirjana AU - Milošević, Emilija AU - Kojić, Snežana PY - 2023 UR - https://zebrafish2023.org/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2023 AB - In our previous work, using transgenic zebrafish line TgBAC(ankrd1a:EGFP), we showed activation of the zebrafish ankrd1a gene in border zone cardiomyocytes of cryoinjured heart and in close proximity of needle-stab wounds in skeletal muscle, indicating its involvement in muscle regeneration. Our results implicated ankrd1a in zebrafish skeletal muscle tissue repair and remodeling, as a sensor of stressed muscle. Here we take a closer look at the spatio-temporal expression profile of the ankrd1a gene in injured zebrafish skeletal muscle by analyzing cryosections prepared from wounded tissue of TgBAC(ankrd1a:EGFP) adults at 1, 3, 5, 7 and 10 days post-injury (dpi). The expression of the fluorescent reporter was observed from 3 dpi and remained until 10 dpi. At 3dpi, new GFP-positive muscle cells emerged inside the injury zone, at the site of needle entry, while in the later days (5, 7 and 10 dpi), newly formed GFP-positive myofibers were visible in the deeper tissue layers within the injury, indicating active repair of the injured tissue. To identify cells in which ankrd1a is activated after injury, we stained the sections for markers of satellite-like cells, undifferentiated and differentiated muscle cells, and mature myofibers. Since the reporter was detected both in the newly formed myofibers that invade the wound and in the apparently uninjured tissue surrounding the injury, we hypothesize that ankrd1a is not only involved in satellite celldependent tissue repair, but its expression might be a hallmark of adaptive process in undamaged myofibers surrounding the physical injury. C3 - 12th European Zebrafish Meeting T1 - Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle EP - 276 SP - 276 SP - 0254 VL - 14 VL - 12 UR - https://hdl.handle.net/21.15107/rcub_imagine_2023 ER -
@conference{ author = "Milovanović, Mina and Bošković, Srđan and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija and Kojić, Snežana", year = "2023", abstract = "In our previous work, using transgenic zebrafish line TgBAC(ankrd1a:EGFP), we showed activation of the zebrafish ankrd1a gene in border zone cardiomyocytes of cryoinjured heart and in close proximity of needle-stab wounds in skeletal muscle, indicating its involvement in muscle regeneration. Our results implicated ankrd1a in zebrafish skeletal muscle tissue repair and remodeling, as a sensor of stressed muscle. Here we take a closer look at the spatio-temporal expression profile of the ankrd1a gene in injured zebrafish skeletal muscle by analyzing cryosections prepared from wounded tissue of TgBAC(ankrd1a:EGFP) adults at 1, 3, 5, 7 and 10 days post-injury (dpi). The expression of the fluorescent reporter was observed from 3 dpi and remained until 10 dpi. At 3dpi, new GFP-positive muscle cells emerged inside the injury zone, at the site of needle entry, while in the later days (5, 7 and 10 dpi), newly formed GFP-positive myofibers were visible in the deeper tissue layers within the injury, indicating active repair of the injured tissue. To identify cells in which ankrd1a is activated after injury, we stained the sections for markers of satellite-like cells, undifferentiated and differentiated muscle cells, and mature myofibers. Since the reporter was detected both in the newly formed myofibers that invade the wound and in the apparently uninjured tissue surrounding the injury, we hypothesize that ankrd1a is not only involved in satellite celldependent tissue repair, but its expression might be a hallmark of adaptive process in undamaged myofibers surrounding the physical injury.", journal = "12th European Zebrafish Meeting", title = "Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle", pages = "276-276-0254", volume = "14, 12", url = "https://hdl.handle.net/21.15107/rcub_imagine_2023" }
Milovanović, M., Bošković, S., Jasnić, J., Novković, M., Milošević, E.,& Kojić, S.. (2023). Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle. in 12th European Zebrafish Meeting, 14, 276-276. https://hdl.handle.net/21.15107/rcub_imagine_2023
Milovanović M, Bošković S, Jasnić J, Novković M, Milošević E, Kojić S. Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle. in 12th European Zebrafish Meeting. 2023;14:276-276. https://hdl.handle.net/21.15107/rcub_imagine_2023 .
Milovanović, Mina, Bošković, Srđan, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, Kojić, Snežana, "Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle" in 12th European Zebrafish Meeting, 14 (2023):276-276, https://hdl.handle.net/21.15107/rcub_imagine_2023 .