Groundwater and soil as a reservoir for polyurethane-degrading bacteria
Аутори
Ćirić, MilicaPantelić, Brana
Šaraba, Vladimir
Nikodinović-Runić, Jasmina
Остала ауторства
Morić, IvanaĐorđević, Valentina
Конференцијски прилог (Објављена верзија)
,
© 2023 Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
Метаподаци
Приказ свих података о документуАпстракт
Plastic waste is a global environmental burden. Polyurethanes (PU), toxic and ubiquitous
synthetic polymers, do not biodegrade quickly, leading to their rapid accumulation in the
soil and water environments. Highly efficient PU-degrading microorganisms are rare in
nature and are of fundamental importance for achieving circular plastic economy. Bacterial
isolates from groundwater, originating from magmatogenic massif and Tertiary basin
within metamorphic area, as well as soil isolates collected from various pristine (PS) and
contaminated sites (CS), were screened using PU model compound Impranil® DLN-SD
(IMP) as sole C source to identify PU-degrading isolates. Phylogenetic analysis of 16S rRNA
gene sequences from IMP-degrading isolates was performed using the neighbor-joining
method to observe their clustering. Thirty one of 96 isolates (32.3 %) from groundwater and
18 of 220 isolates (8.2%) from soil produced prominent IMP-clearing zones. Thirteen IMPdegrading
isolates from ...each type of environment, belonging to 8 genera (Pseudomonas,
Proteus, Enterobacter, Flavobacterium, Serratia, Pantoea, Acinetobacter and Stenotrophomonas)
for groundwater and to 6 genera (Streptomyces, Pseudomonas, Rhodococcus, Achromobacter,
Bacillus and Paenibacillus) for soil environment, were included in phylogenetic analysis. No
clear grouping of groundwater and soil isolates was observed, indicating that isolates are
too distinct. Stronger clustering was observed for groundwater compared to soil isolates. For
groundwater, strongest clustering was observed for 2 isolates belonging to Proteus genus,
2 belonging to Flavobacterium and 2 to Pseudomonas. For soil samples, strongest clustering
was observed for 3 isolates belonging to genus Streptomyces. There was no clear grouping
within isolates from CS and PS. In the future, wider range of environmental niches should be
included in screening efforts for development of biocatalytic processes for management of
plastic waste. Subterranean ecosystems, which are not readily accessible for sampling and
represent largely unexplored reservoir of biotechnologically relevant enzymatic activities,
should also be more represented in such screenings.
Кључне речи:
groundwater / soil / polyurethane-degrading bacteria / 16S phylogenyИзвор:
4th Belgrade Bioinformatics Conference, 2023, 4, 99-99Издавач:
- Belgrade : Institute of molecular genetics and genetic engineering
Финансирање / пројекти:
- EU H2020 Research and Innovation Programme (grant agreement No. 870292, BioICEP)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Напомена:
- Book of abstract: 4th Belgrade Bioinformatics Conference, June 19-23, 2023
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Ćirić, Milica AU - Pantelić, Brana AU - Šaraba, Vladimir AU - Nikodinović-Runić, Jasmina PY - 2023 UR - https://belbi.bg.ac.rs/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2044 AB - Plastic waste is a global environmental burden. Polyurethanes (PU), toxic and ubiquitous synthetic polymers, do not biodegrade quickly, leading to their rapid accumulation in the soil and water environments. Highly efficient PU-degrading microorganisms are rare in nature and are of fundamental importance for achieving circular plastic economy. Bacterial isolates from groundwater, originating from magmatogenic massif and Tertiary basin within metamorphic area, as well as soil isolates collected from various pristine (PS) and contaminated sites (CS), were screened using PU model compound Impranil® DLN-SD (IMP) as sole C source to identify PU-degrading isolates. Phylogenetic analysis of 16S rRNA gene sequences from IMP-degrading isolates was performed using the neighbor-joining method to observe their clustering. Thirty one of 96 isolates (32.3 %) from groundwater and 18 of 220 isolates (8.2%) from soil produced prominent IMP-clearing zones. Thirteen IMPdegrading isolates from each type of environment, belonging to 8 genera (Pseudomonas, Proteus, Enterobacter, Flavobacterium, Serratia, Pantoea, Acinetobacter and Stenotrophomonas) for groundwater and to 6 genera (Streptomyces, Pseudomonas, Rhodococcus, Achromobacter, Bacillus and Paenibacillus) for soil environment, were included in phylogenetic analysis. No clear grouping of groundwater and soil isolates was observed, indicating that isolates are too distinct. Stronger clustering was observed for groundwater compared to soil isolates. For groundwater, strongest clustering was observed for 2 isolates belonging to Proteus genus, 2 belonging to Flavobacterium and 2 to Pseudomonas. For soil samples, strongest clustering was observed for 3 isolates belonging to genus Streptomyces. There was no clear grouping within isolates from CS and PS. In the future, wider range of environmental niches should be included in screening efforts for development of biocatalytic processes for management of plastic waste. Subterranean ecosystems, which are not readily accessible for sampling and represent largely unexplored reservoir of biotechnologically relevant enzymatic activities, should also be more represented in such screenings. PB - Belgrade : Institute of molecular genetics and genetic engineering C3 - 4th Belgrade Bioinformatics Conference T1 - Groundwater and soil as a reservoir for polyurethane-degrading bacteria EP - 99 SP - 99 VL - 4 UR - https://hdl.handle.net/21.15107/rcub_imagine_2044 ER -
@conference{ author = "Ćirić, Milica and Pantelić, Brana and Šaraba, Vladimir and Nikodinović-Runić, Jasmina", year = "2023", abstract = "Plastic waste is a global environmental burden. Polyurethanes (PU), toxic and ubiquitous synthetic polymers, do not biodegrade quickly, leading to their rapid accumulation in the soil and water environments. Highly efficient PU-degrading microorganisms are rare in nature and are of fundamental importance for achieving circular plastic economy. Bacterial isolates from groundwater, originating from magmatogenic massif and Tertiary basin within metamorphic area, as well as soil isolates collected from various pristine (PS) and contaminated sites (CS), were screened using PU model compound Impranil® DLN-SD (IMP) as sole C source to identify PU-degrading isolates. Phylogenetic analysis of 16S rRNA gene sequences from IMP-degrading isolates was performed using the neighbor-joining method to observe their clustering. Thirty one of 96 isolates (32.3 %) from groundwater and 18 of 220 isolates (8.2%) from soil produced prominent IMP-clearing zones. Thirteen IMPdegrading isolates from each type of environment, belonging to 8 genera (Pseudomonas, Proteus, Enterobacter, Flavobacterium, Serratia, Pantoea, Acinetobacter and Stenotrophomonas) for groundwater and to 6 genera (Streptomyces, Pseudomonas, Rhodococcus, Achromobacter, Bacillus and Paenibacillus) for soil environment, were included in phylogenetic analysis. No clear grouping of groundwater and soil isolates was observed, indicating that isolates are too distinct. Stronger clustering was observed for groundwater compared to soil isolates. For groundwater, strongest clustering was observed for 2 isolates belonging to Proteus genus, 2 belonging to Flavobacterium and 2 to Pseudomonas. For soil samples, strongest clustering was observed for 3 isolates belonging to genus Streptomyces. There was no clear grouping within isolates from CS and PS. In the future, wider range of environmental niches should be included in screening efforts for development of biocatalytic processes for management of plastic waste. Subterranean ecosystems, which are not readily accessible for sampling and represent largely unexplored reservoir of biotechnologically relevant enzymatic activities, should also be more represented in such screenings.", publisher = "Belgrade : Institute of molecular genetics and genetic engineering", journal = "4th Belgrade Bioinformatics Conference", title = "Groundwater and soil as a reservoir for polyurethane-degrading bacteria", pages = "99-99", volume = "4", url = "https://hdl.handle.net/21.15107/rcub_imagine_2044" }
Ćirić, M., Pantelić, B., Šaraba, V.,& Nikodinović-Runić, J.. (2023). Groundwater and soil as a reservoir for polyurethane-degrading bacteria. in 4th Belgrade Bioinformatics Conference Belgrade : Institute of molecular genetics and genetic engineering., 4, 99-99. https://hdl.handle.net/21.15107/rcub_imagine_2044
Ćirić M, Pantelić B, Šaraba V, Nikodinović-Runić J. Groundwater and soil as a reservoir for polyurethane-degrading bacteria. in 4th Belgrade Bioinformatics Conference. 2023;4:99-99. https://hdl.handle.net/21.15107/rcub_imagine_2044 .
Ćirić, Milica, Pantelić, Brana, Šaraba, Vladimir, Nikodinović-Runić, Jasmina, "Groundwater and soil as a reservoir for polyurethane-degrading bacteria" in 4th Belgrade Bioinformatics Conference, 4 (2023):99-99, https://hdl.handle.net/21.15107/rcub_imagine_2044 .