The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro
Аутори
Bojić, LukaSchwirtlich, Marija
Lazić, Stefan
Stanisavljević Ninković, Danijela
Balint, Vanda
Stevanović, Milena
Milivojević, Milena
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: Prolonged exposure to sunlight, has a harmful effect on skin cells encompassing reduced
viability, morphological changes, and altered gene expression. The two most prevalent types ofskin cancer,squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC), arise from malignant transformation
of keratinocytes. UV radiation, among other factors, serves as the primary cause of these tumors. Previous data hasshown that changesin different SOX genes expression in these cancer types correlates with
disease progression, suggesting their role as oncogenes/tumor suppressors. The presented work is focused on examining the impact of UVB radiation on the expression of SOX2 and SOX9 genesin HaCaT cells
derived from human keratinocytes.
Methods: Using a custom-made UV solarsimulator for the irradiation of HaCaT cells with 150 mJ/cm2 or
300 mJ/cm2
, we analyzed SOX2 and SOX9 gene expression. In order to determine the protective effects
of quercetin, anti-inflammatory bioflavonoi...d, we treated irradiated HaCaT with quercetin, and analyzed
SOX gene expression.
Results: Our resultsindicate that UVB radiation induces a dose dependent decrease of SOX2 expression
while expression of SOX9 was increased at the dose of 150 mJ/cm2 in HaCaT. Treatment of cells with
quercetin increased the expression of both SOX2 and SOX9 genesin HaCaT cellsfollowing UVB radiation
at both doses compared to irradiated cells.
Conclusions: Further research is needed to understand the molecular mechanisms and significance of
SOX2 and SOX9 in UVB-induced cellular responses, in the context of nonmelanoma cancers with potential implications for targeted therapeutic strategies for nonmelanoma cancers
Кључне речи:
UVB / SOX2 / SOX9 / HaCaT cellsИзвор:
CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia, 2023, 143-143Издавач:
- Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Bojić, Luka AU - Schwirtlich, Marija AU - Lazić, Stefan AU - Stanisavljević Ninković, Danijela AU - Balint, Vanda AU - Stevanović, Milena AU - Milivojević, Milena PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2133 AB - Introduction: Prolonged exposure to sunlight, has a harmful effect on skin cells encompassing reduced viability, morphological changes, and altered gene expression. The two most prevalent types ofskin cancer,squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC), arise from malignant transformation of keratinocytes. UV radiation, among other factors, serves as the primary cause of these tumors. Previous data hasshown that changesin different SOX genes expression in these cancer types correlates with disease progression, suggesting their role as oncogenes/tumor suppressors. The presented work is focused on examining the impact of UVB radiation on the expression of SOX2 and SOX9 genesin HaCaT cells derived from human keratinocytes. Methods: Using a custom-made UV solarsimulator for the irradiation of HaCaT cells with 150 mJ/cm2 or 300 mJ/cm2 , we analyzed SOX2 and SOX9 gene expression. In order to determine the protective effects of quercetin, anti-inflammatory bioflavonoid, we treated irradiated HaCaT with quercetin, and analyzed SOX gene expression. Results: Our resultsindicate that UVB radiation induces a dose dependent decrease of SOX2 expression while expression of SOX9 was increased at the dose of 150 mJ/cm2 in HaCaT. Treatment of cells with quercetin increased the expression of both SOX2 and SOX9 genesin HaCaT cellsfollowing UVB radiation at both doses compared to irradiated cells. Conclusions: Further research is needed to understand the molecular mechanisms and significance of SOX2 and SOX9 in UVB-induced cellular responses, in the context of nonmelanoma cancers with potential implications for targeted therapeutic strategies for nonmelanoma cancers PB - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade C3 - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia T1 - The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro EP - 143 SP - 143 UR - https://hdl.handle.net/21.15107/rcub_imagine_2133 ER -
@conference{ author = "Bojić, Luka and Schwirtlich, Marija and Lazić, Stefan and Stanisavljević Ninković, Danijela and Balint, Vanda and Stevanović, Milena and Milivojević, Milena", year = "2023", abstract = "Introduction: Prolonged exposure to sunlight, has a harmful effect on skin cells encompassing reduced viability, morphological changes, and altered gene expression. The two most prevalent types ofskin cancer,squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC), arise from malignant transformation of keratinocytes. UV radiation, among other factors, serves as the primary cause of these tumors. Previous data hasshown that changesin different SOX genes expression in these cancer types correlates with disease progression, suggesting their role as oncogenes/tumor suppressors. The presented work is focused on examining the impact of UVB radiation on the expression of SOX2 and SOX9 genesin HaCaT cells derived from human keratinocytes. Methods: Using a custom-made UV solarsimulator for the irradiation of HaCaT cells with 150 mJ/cm2 or 300 mJ/cm2 , we analyzed SOX2 and SOX9 gene expression. In order to determine the protective effects of quercetin, anti-inflammatory bioflavonoid, we treated irradiated HaCaT with quercetin, and analyzed SOX gene expression. Results: Our resultsindicate that UVB radiation induces a dose dependent decrease of SOX2 expression while expression of SOX9 was increased at the dose of 150 mJ/cm2 in HaCaT. Treatment of cells with quercetin increased the expression of both SOX2 and SOX9 genesin HaCaT cellsfollowing UVB radiation at both doses compared to irradiated cells. Conclusions: Further research is needed to understand the molecular mechanisms and significance of SOX2 and SOX9 in UVB-induced cellular responses, in the context of nonmelanoma cancers with potential implications for targeted therapeutic strategies for nonmelanoma cancers", publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade", journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia", title = "The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro", pages = "143-143", url = "https://hdl.handle.net/21.15107/rcub_imagine_2133" }
Bojić, L., Schwirtlich, M., Lazić, S., Stanisavljević Ninković, D., Balint, V., Stevanović, M.,& Milivojević, M.. (2023). The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 143-143. https://hdl.handle.net/21.15107/rcub_imagine_2133
Bojić L, Schwirtlich M, Lazić S, Stanisavljević Ninković D, Balint V, Stevanović M, Milivojević M. The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:143-143. https://hdl.handle.net/21.15107/rcub_imagine_2133 .
Bojić, Luka, Schwirtlich, Marija, Lazić, Stefan, Stanisavljević Ninković, Danijela, Balint, Vanda, Stevanović, Milena, Milivojević, Milena, "The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):143-143, https://hdl.handle.net/21.15107/rcub_imagine_2133 .