Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds
Аутори
Pejić, JelenaMojsin, Marija
Stojkovska, Jasmina
Medić, Aleksandra
Petrović, Isidora
Stevanović, Milena
Obradović, Bojana
Milivojević, Milena
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: The NT2/D1 embryonal carcinoma cell line represents a well-established in vitro model of
human neurogenesis. It’s widely used for studying neurodevelopmental processes, neurotoxicity, and
neurodegenerative disorders. The utilization of alginate fibers as a 3D cell culture system offers a biocompatible and structurally supportive environment for neural differentiation and maturation of cells,
making it a suitable tool for investigating neurodevelopmental processes.
Methods: In thisstudy, we evaluated the alginate microfibers as a 3D modelsystem for in vitro neural differentiation of NT2/D1 cells.We described the immobilization of NT2/D1 cellsin alginate microfibers and
the effect of propagation in this 3D model on morphological features, viability, and proliferation of immobilized cells. We also assessed the RA-induced initiation of neural differentiation of NT2/D1 cellsin alginate microfibers by comparison with the initiation of neural differentiation in adherent 2D ...cell culture.
Results: Our results showed that immobilized NT2/D1 acquired morphological features characteristic
of cells propagated in 3D model systems and retain viability, proliferative capacity, and ability to attach
to adherent surfaces. In addition, immobilized NT2/D1 cells preserved neural differentiation capacity.
Upon RA induction we detected a marked decrease in the expression of specific pluripotency-maintaining markers, SOX2, OCT4, and NANOG. Consecutively, the expression of early neural markers, SOX3,
PAX6, and miR219 was significantly increased.
Conclusion: Neural differentiation of NT2/D1 cellsimmobilized within alginate fibersrepresents a highly
promising 3D modelsystem forstudying human neurogenesis and offers a valuable platform forscreening the effect of drugs and bioactive compounds on human neural differentiation.
Кључне речи:
NT2/D1 cell line / neural differentiation / alginate fibers / 3D model systemИзвор:
CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia, 2023, 113-113Издавач:
- Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- European Union`s Horizon Europe programme (Grant Agreement Number 101060201, STREAMLINE)
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Pejić, Jelena AU - Mojsin, Marija AU - Stojkovska, Jasmina AU - Medić, Aleksandra AU - Petrović, Isidora AU - Stevanović, Milena AU - Obradović, Bojana AU - Milivojević, Milena PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2151 AB - Introduction: The NT2/D1 embryonal carcinoma cell line represents a well-established in vitro model of human neurogenesis. It’s widely used for studying neurodevelopmental processes, neurotoxicity, and neurodegenerative disorders. The utilization of alginate fibers as a 3D cell culture system offers a biocompatible and structurally supportive environment for neural differentiation and maturation of cells, making it a suitable tool for investigating neurodevelopmental processes. Methods: In thisstudy, we evaluated the alginate microfibers as a 3D modelsystem for in vitro neural differentiation of NT2/D1 cells.We described the immobilization of NT2/D1 cellsin alginate microfibers and the effect of propagation in this 3D model on morphological features, viability, and proliferation of immobilized cells. We also assessed the RA-induced initiation of neural differentiation of NT2/D1 cellsin alginate microfibers by comparison with the initiation of neural differentiation in adherent 2D cell culture. Results: Our results showed that immobilized NT2/D1 acquired morphological features characteristic of cells propagated in 3D model systems and retain viability, proliferative capacity, and ability to attach to adherent surfaces. In addition, immobilized NT2/D1 cells preserved neural differentiation capacity. Upon RA induction we detected a marked decrease in the expression of specific pluripotency-maintaining markers, SOX2, OCT4, and NANOG. Consecutively, the expression of early neural markers, SOX3, PAX6, and miR219 was significantly increased. Conclusion: Neural differentiation of NT2/D1 cellsimmobilized within alginate fibersrepresents a highly promising 3D modelsystem forstudying human neurogenesis and offers a valuable platform forscreening the effect of drugs and bioactive compounds on human neural differentiation. PB - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade C3 - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia T1 - Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds EP - 113 SP - 113 UR - https://hdl.handle.net/21.15107/rcub_imagine_2151 ER -
@conference{ author = "Pejić, Jelena and Mojsin, Marija and Stojkovska, Jasmina and Medić, Aleksandra and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana and Milivojević, Milena", year = "2023", abstract = "Introduction: The NT2/D1 embryonal carcinoma cell line represents a well-established in vitro model of human neurogenesis. It’s widely used for studying neurodevelopmental processes, neurotoxicity, and neurodegenerative disorders. The utilization of alginate fibers as a 3D cell culture system offers a biocompatible and structurally supportive environment for neural differentiation and maturation of cells, making it a suitable tool for investigating neurodevelopmental processes. Methods: In thisstudy, we evaluated the alginate microfibers as a 3D modelsystem for in vitro neural differentiation of NT2/D1 cells.We described the immobilization of NT2/D1 cellsin alginate microfibers and the effect of propagation in this 3D model on morphological features, viability, and proliferation of immobilized cells. We also assessed the RA-induced initiation of neural differentiation of NT2/D1 cellsin alginate microfibers by comparison with the initiation of neural differentiation in adherent 2D cell culture. Results: Our results showed that immobilized NT2/D1 acquired morphological features characteristic of cells propagated in 3D model systems and retain viability, proliferative capacity, and ability to attach to adherent surfaces. In addition, immobilized NT2/D1 cells preserved neural differentiation capacity. Upon RA induction we detected a marked decrease in the expression of specific pluripotency-maintaining markers, SOX2, OCT4, and NANOG. Consecutively, the expression of early neural markers, SOX3, PAX6, and miR219 was significantly increased. Conclusion: Neural differentiation of NT2/D1 cellsimmobilized within alginate fibersrepresents a highly promising 3D modelsystem forstudying human neurogenesis and offers a valuable platform forscreening the effect of drugs and bioactive compounds on human neural differentiation.", publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade", journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia", title = "Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds", pages = "113-113", url = "https://hdl.handle.net/21.15107/rcub_imagine_2151" }
Pejić, J., Mojsin, M., Stojkovska, J., Medić, A., Petrović, I., Stevanović, M., Obradović, B.,& Milivojević, M.. (2023). Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 113-113. https://hdl.handle.net/21.15107/rcub_imagine_2151
Pejić J, Mojsin M, Stojkovska J, Medić A, Petrović I, Stevanović M, Obradović B, Milivojević M. Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:113-113. https://hdl.handle.net/21.15107/rcub_imagine_2151 .
Pejić, Jelena, Mojsin, Marija, Stojkovska, Jasmina, Medić, Aleksandra, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, Milivojević, Milena, "Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):113-113, https://hdl.handle.net/21.15107/rcub_imagine_2151 .