Determination of hydrogen cyanide producing strains as potential biocontrol agents
Autori
Mesaroš, AleksandraAtanasković, Iva
Jakovljević, Stefan
Stević, Tatjana
Dinić, Miroslav
Lozo, Jelena
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Introduction: Hydrogen cyanide (HCN) is a volatile secondary metabolite synthesized by some bacteria, and this ability enablestheir activity against various pathogens. The aim of thisstudy wasto identify
HCN-producing bacteria and investigate their biocontrol potential.
Methods: Three HCN-producing strains were detected in a collection of bell pepper plant isolates using
a semi-quantitative assay with picric acid. The presence of hcnABC operon genes was confirmed by PCR.
The biological control potential of the HCN-producing strains wastested against three fungal (Fusarium
oxysporum, Rhizoctonia solani, Verticillium dahliae) and eight bacterial (genera Xanthomonas,
Pseudomonas and Clavibacter) pathogens of bell pepper plants in a split-section Petri dish experiment.
The potential nematocidal activity was demonstrated by using the Caenorhabditis elegans AU37 strain,
with temperature-sensitive sterility and enhanced sensitivity to pathogens.
Results: Detailed characterization of ...300 isolates from our collection revealed that we have three different HCN-producing strains identified as Bacillussubtilis, Pseudomonas moraviensis, and P. putida, with
P. putida A32 being the most potent. Thisstrain is used for the deletion of the hcnB gene to confirm HCN
as a biocontrol agent.
Conclusion: The HCN-producing strains showed biocontrol potential against bacteria, fungi, and nematodes. It is concluded that the biological control activity isthe result of a volatile metabolite diffusing
through the air. Our future exp
Ključne reči:
plant pathogens / hydrogen cyanide (HCN) / biological controlIzvor:
CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia, 2023, 116-116Izdavač:
- Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200178 (Univerzitet u Beogradu, Biološki fakultet) (RS-MESTD-inst-2020-200178)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Mesaroš, Aleksandra AU - Atanasković, Iva AU - Jakovljević, Stefan AU - Stević, Tatjana AU - Dinić, Miroslav AU - Lozo, Jelena PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2152 AB - Introduction: Hydrogen cyanide (HCN) is a volatile secondary metabolite synthesized by some bacteria, and this ability enablestheir activity against various pathogens. The aim of thisstudy wasto identify HCN-producing bacteria and investigate their biocontrol potential. Methods: Three HCN-producing strains were detected in a collection of bell pepper plant isolates using a semi-quantitative assay with picric acid. The presence of hcnABC operon genes was confirmed by PCR. The biological control potential of the HCN-producing strains wastested against three fungal (Fusarium oxysporum, Rhizoctonia solani, Verticillium dahliae) and eight bacterial (genera Xanthomonas, Pseudomonas and Clavibacter) pathogens of bell pepper plants in a split-section Petri dish experiment. The potential nematocidal activity was demonstrated by using the Caenorhabditis elegans AU37 strain, with temperature-sensitive sterility and enhanced sensitivity to pathogens. Results: Detailed characterization of 300 isolates from our collection revealed that we have three different HCN-producing strains identified as Bacillussubtilis, Pseudomonas moraviensis, and P. putida, with P. putida A32 being the most potent. Thisstrain is used for the deletion of the hcnB gene to confirm HCN as a biocontrol agent. Conclusion: The HCN-producing strains showed biocontrol potential against bacteria, fungi, and nematodes. It is concluded that the biological control activity isthe result of a volatile metabolite diffusing through the air. Our future exp PB - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade C3 - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia T1 - Determination of hydrogen cyanide producing strains as potential biocontrol agents EP - 116 SP - 116 UR - https://hdl.handle.net/21.15107/rcub_imagine_2152 ER -
@conference{ author = "Mesaroš, Aleksandra and Atanasković, Iva and Jakovljević, Stefan and Stević, Tatjana and Dinić, Miroslav and Lozo, Jelena", year = "2023", abstract = "Introduction: Hydrogen cyanide (HCN) is a volatile secondary metabolite synthesized by some bacteria, and this ability enablestheir activity against various pathogens. The aim of thisstudy wasto identify HCN-producing bacteria and investigate their biocontrol potential. Methods: Three HCN-producing strains were detected in a collection of bell pepper plant isolates using a semi-quantitative assay with picric acid. The presence of hcnABC operon genes was confirmed by PCR. The biological control potential of the HCN-producing strains wastested against three fungal (Fusarium oxysporum, Rhizoctonia solani, Verticillium dahliae) and eight bacterial (genera Xanthomonas, Pseudomonas and Clavibacter) pathogens of bell pepper plants in a split-section Petri dish experiment. The potential nematocidal activity was demonstrated by using the Caenorhabditis elegans AU37 strain, with temperature-sensitive sterility and enhanced sensitivity to pathogens. Results: Detailed characterization of 300 isolates from our collection revealed that we have three different HCN-producing strains identified as Bacillussubtilis, Pseudomonas moraviensis, and P. putida, with P. putida A32 being the most potent. Thisstrain is used for the deletion of the hcnB gene to confirm HCN as a biocontrol agent. Conclusion: The HCN-producing strains showed biocontrol potential against bacteria, fungi, and nematodes. It is concluded that the biological control activity isthe result of a volatile metabolite diffusing through the air. Our future exp", publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade", journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia", title = "Determination of hydrogen cyanide producing strains as potential biocontrol agents", pages = "116-116", url = "https://hdl.handle.net/21.15107/rcub_imagine_2152" }
Mesaroš, A., Atanasković, I., Jakovljević, S., Stević, T., Dinić, M.,& Lozo, J.. (2023). Determination of hydrogen cyanide producing strains as potential biocontrol agents. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 116-116. https://hdl.handle.net/21.15107/rcub_imagine_2152
Mesaroš A, Atanasković I, Jakovljević S, Stević T, Dinić M, Lozo J. Determination of hydrogen cyanide producing strains as potential biocontrol agents. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:116-116. https://hdl.handle.net/21.15107/rcub_imagine_2152 .
Mesaroš, Aleksandra, Atanasković, Iva, Jakovljević, Stefan, Stević, Tatjana, Dinić, Miroslav, Lozo, Jelena, "Determination of hydrogen cyanide producing strains as potential biocontrol agents" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):116-116, https://hdl.handle.net/21.15107/rcub_imagine_2152 .