NANOMATERIALS-BASED STRATEGY FOR MYELOID CELLS ACTIVATION RESULTS IN EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS AMELIORATION AND GUT MICROBIOTA MODULATION
Аутори
Radojević, DušanBekić, Marina
Ilić, Nataša
Đokić, Jelena
Stojanović, Dušica
Vasilev, Saša
Gruden-Movsesijan, Alisa
Tomić, Sergej
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
ntroduction: Recent studies implicated overactivated myeloid cells and gut microbiome, along with our work,
in multiple sclerosis (MS) pathogenesis. As we have shown before, prostaglandin (PG)E2 promotes
suppressive properties of myeloid cells leading to amelioration of symptoms in myelin oligodendrocyte
glycoprotein
(MOG)-induced experimental autoimmune encephalomyelitis
(EAE). Additionally, we
investigated how the changes of gut microbiota associate with EAE and the effects of therapy.
Materials & Methods: MOG35-55 in Complete Freund Adjuvans was used for EAE induction in C57BL/6
mice. Gold nanoparticles (GNP) conjugated with PGE2 and MOG were applied on the day 1, 3, 5, 7, and 9
post-immunization. We performed extensive immunophenotyping and metagenomic analysis in order to
decipher association between gut microbiome and efficacy of GNP-MOG-PGE2 treatment.
Results: GNP-MOG-PGE2 treatment alleviates EAE symptoms, decreased levels of pro-inflammatory
cytokines in... sera, and increased proportion of suppressive MDSCs in CNS-infiltrates. Furthermore, EAE
induction significantly affected species richness, while GNP-MOG-PGE2 treatment increased the gut
microbiota diversity and preserved the richness of species with immunomodulatory properties.
Conclusion: Taken together, our data indicate that targeted activation of myeloid cells by GNP-MOG-PGE2
together with gut microbiota modification is very promising therapeutic strategy for MS.
Извор:
10th ISM World Congress on Targeting Microbiota, 2023, 77-77Финансирање / пројекти:
- Nano-MDSC-Thera - Novel Immunotherapeutic Approaches for Autoimmune Diseases Based on Myeloid Derived Suppressor Cells Induced By Nanomaterials (RS-ScienceFundRS-Promis-6062673)
Напомена:
- International Society of Microbiota 10th ISM World Congress on Targeting Microbiota October 17-19, 2023 – Venice, Italy
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Radojević, Dušan AU - Bekić, Marina AU - Ilić, Nataša AU - Đokić, Jelena AU - Stojanović, Dušica AU - Vasilev, Saša AU - Gruden-Movsesijan, Alisa AU - Tomić, Sergej PY - 2023 UR - https://www.microbiota-site.com/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2188 AB - ntroduction: Recent studies implicated overactivated myeloid cells and gut microbiome, along with our work, in multiple sclerosis (MS) pathogenesis. As we have shown before, prostaglandin (PG)E2 promotes suppressive properties of myeloid cells leading to amelioration of symptoms in myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE). Additionally, we investigated how the changes of gut microbiota associate with EAE and the effects of therapy. Materials & Methods: MOG35-55 in Complete Freund Adjuvans was used for EAE induction in C57BL/6 mice. Gold nanoparticles (GNP) conjugated with PGE2 and MOG were applied on the day 1, 3, 5, 7, and 9 post-immunization. We performed extensive immunophenotyping and metagenomic analysis in order to decipher association between gut microbiome and efficacy of GNP-MOG-PGE2 treatment. Results: GNP-MOG-PGE2 treatment alleviates EAE symptoms, decreased levels of pro-inflammatory cytokines in sera, and increased proportion of suppressive MDSCs in CNS-infiltrates. Furthermore, EAE induction significantly affected species richness, while GNP-MOG-PGE2 treatment increased the gut microbiota diversity and preserved the richness of species with immunomodulatory properties. Conclusion: Taken together, our data indicate that targeted activation of myeloid cells by GNP-MOG-PGE2 together with gut microbiota modification is very promising therapeutic strategy for MS. C3 - 10th ISM World Congress on Targeting Microbiota T1 - NANOMATERIALS-BASED STRATEGY FOR MYELOID CELLS ACTIVATION RESULTS IN EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS AMELIORATION AND GUT MICROBIOTA MODULATION EP - 77 SP - 77 UR - https://hdl.handle.net/21.15107/rcub_imagine_2188 ER -
@conference{ author = "Radojević, Dušan and Bekić, Marina and Ilić, Nataša and Đokić, Jelena and Stojanović, Dušica and Vasilev, Saša and Gruden-Movsesijan, Alisa and Tomić, Sergej", year = "2023", abstract = "ntroduction: Recent studies implicated overactivated myeloid cells and gut microbiome, along with our work, in multiple sclerosis (MS) pathogenesis. As we have shown before, prostaglandin (PG)E2 promotes suppressive properties of myeloid cells leading to amelioration of symptoms in myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE). Additionally, we investigated how the changes of gut microbiota associate with EAE and the effects of therapy. Materials & Methods: MOG35-55 in Complete Freund Adjuvans was used for EAE induction in C57BL/6 mice. Gold nanoparticles (GNP) conjugated with PGE2 and MOG were applied on the day 1, 3, 5, 7, and 9 post-immunization. We performed extensive immunophenotyping and metagenomic analysis in order to decipher association between gut microbiome and efficacy of GNP-MOG-PGE2 treatment. Results: GNP-MOG-PGE2 treatment alleviates EAE symptoms, decreased levels of pro-inflammatory cytokines in sera, and increased proportion of suppressive MDSCs in CNS-infiltrates. Furthermore, EAE induction significantly affected species richness, while GNP-MOG-PGE2 treatment increased the gut microbiota diversity and preserved the richness of species with immunomodulatory properties. Conclusion: Taken together, our data indicate that targeted activation of myeloid cells by GNP-MOG-PGE2 together with gut microbiota modification is very promising therapeutic strategy for MS.", journal = "10th ISM World Congress on Targeting Microbiota", title = "NANOMATERIALS-BASED STRATEGY FOR MYELOID CELLS ACTIVATION RESULTS IN EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS AMELIORATION AND GUT MICROBIOTA MODULATION", pages = "77-77", url = "https://hdl.handle.net/21.15107/rcub_imagine_2188" }
Radojević, D., Bekić, M., Ilić, N., Đokić, J., Stojanović, D., Vasilev, S., Gruden-Movsesijan, A.,& Tomić, S.. (2023). NANOMATERIALS-BASED STRATEGY FOR MYELOID CELLS ACTIVATION RESULTS IN EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS AMELIORATION AND GUT MICROBIOTA MODULATION. in 10th ISM World Congress on Targeting Microbiota, 77-77. https://hdl.handle.net/21.15107/rcub_imagine_2188
Radojević D, Bekić M, Ilić N, Đokić J, Stojanović D, Vasilev S, Gruden-Movsesijan A, Tomić S. NANOMATERIALS-BASED STRATEGY FOR MYELOID CELLS ACTIVATION RESULTS IN EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS AMELIORATION AND GUT MICROBIOTA MODULATION. in 10th ISM World Congress on Targeting Microbiota. 2023;:77-77. https://hdl.handle.net/21.15107/rcub_imagine_2188 .
Radojević, Dušan, Bekić, Marina, Ilić, Nataša, Đokić, Jelena, Stojanović, Dušica, Vasilev, Saša, Gruden-Movsesijan, Alisa, Tomić, Sergej, "NANOMATERIALS-BASED STRATEGY FOR MYELOID CELLS ACTIVATION RESULTS IN EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS AMELIORATION AND GUT MICROBIOTA MODULATION" in 10th ISM World Congress on Targeting Microbiota (2023):77-77, https://hdl.handle.net/21.15107/rcub_imagine_2188 .