Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21
Аутори
Lazić, StefanStanisavljević Ninković, Danijela
Petrović, Isidora
Aleksandra, Medić
Milivojević, Milena
Bojić, Luka
Erceg, Slaven
Stevanović, Milena
Švirtlih, Marija
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Brain trauma leads to the induction of neural stem cell proliferation and the migration
of young neurons to injured areas. However, these neurons are insufficient to fully
restore neuronal function due to the limited potential of adult neurogenesis. This study
aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum
of brain pathologies, on pluripotency and the capacity of stem cells to differentiate
into neural progenitors. We analyzed the expression of SOX genes and microRNAs as
they control a variety of cellular processes during neuronal differentiation, including
cell proliferation and cell fate determination. In vitro neuronal differentiation of
human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells
were used as a model system of adult neurogenesis. Cobalt chloride was used to
induce hypoxia.
The results of the analysis showed that, following hypoxia, the efficiency of neuronal
induction was significantly decreased,... that coincident with decline in mRNA
expression levels of SOXB and SOXC genes. In contrast to that, the expression level of
miR-21 was significantly increased.
Our findings advance the study of SOX TFs, miR-21, and their possible interplay in
ischemia-related pathologies, establishing them as prospective biomarkers and
possible targets for future diagnostic and therapeutic approaches.
Извор:
8th Congress of the Serbian Neuroscience Society, 2023, 96-96Издавач:
- Belgrade : Serbian Neuroscience Society
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- Strategic project of Serbian Academy of Arts and Sciences (F172)
- Science Fund of the Republic of Serbia, Serbian Science and Diaspora Collaboration Program: Knowledge Exchange Vouchers, (6436225)
Напомена:
- BOOK OF ABSTRACTS: 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Lazić, Stefan AU - Stanisavljević Ninković, Danijela AU - Petrović, Isidora AU - Aleksandra, Medić AU - Milivojević, Milena AU - Bojić, Luka AU - Erceg, Slaven AU - Stevanović, Milena AU - Švirtlih, Marija PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2287 AB - Brain trauma leads to the induction of neural stem cell proliferation and the migration of young neurons to injured areas. However, these neurons are insufficient to fully restore neuronal function due to the limited potential of adult neurogenesis. This study aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum of brain pathologies, on pluripotency and the capacity of stem cells to differentiate into neural progenitors. We analyzed the expression of SOX genes and microRNAs as they control a variety of cellular processes during neuronal differentiation, including cell proliferation and cell fate determination. In vitro neuronal differentiation of human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells were used as a model system of adult neurogenesis. Cobalt chloride was used to induce hypoxia. The results of the analysis showed that, following hypoxia, the efficiency of neuronal induction was significantly decreased, that coincident with decline in mRNA expression levels of SOXB and SOXC genes. In contrast to that, the expression level of miR-21 was significantly increased. Our findings advance the study of SOX TFs, miR-21, and their possible interplay in ischemia-related pathologies, establishing them as prospective biomarkers and possible targets for future diagnostic and therapeutic approaches. PB - Belgrade : Serbian Neuroscience Society C3 - 8th Congress of the Serbian Neuroscience Society T1 - Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21 EP - 96 SP - 96 UR - https://hdl.handle.net/21.15107/rcub_imagine_2287 ER -
@conference{ author = "Lazić, Stefan and Stanisavljević Ninković, Danijela and Petrović, Isidora and Aleksandra, Medić and Milivojević, Milena and Bojić, Luka and Erceg, Slaven and Stevanović, Milena and Švirtlih, Marija", year = "2023", abstract = "Brain trauma leads to the induction of neural stem cell proliferation and the migration of young neurons to injured areas. However, these neurons are insufficient to fully restore neuronal function due to the limited potential of adult neurogenesis. This study aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum of brain pathologies, on pluripotency and the capacity of stem cells to differentiate into neural progenitors. We analyzed the expression of SOX genes and microRNAs as they control a variety of cellular processes during neuronal differentiation, including cell proliferation and cell fate determination. In vitro neuronal differentiation of human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells were used as a model system of adult neurogenesis. Cobalt chloride was used to induce hypoxia. The results of the analysis showed that, following hypoxia, the efficiency of neuronal induction was significantly decreased, that coincident with decline in mRNA expression levels of SOXB and SOXC genes. In contrast to that, the expression level of miR-21 was significantly increased. Our findings advance the study of SOX TFs, miR-21, and their possible interplay in ischemia-related pathologies, establishing them as prospective biomarkers and possible targets for future diagnostic and therapeutic approaches.", publisher = "Belgrade : Serbian Neuroscience Society", journal = "8th Congress of the Serbian Neuroscience Society", title = "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21", pages = "96-96", url = "https://hdl.handle.net/21.15107/rcub_imagine_2287" }
Lazić, S., Stanisavljević Ninković, D., Petrović, I., Aleksandra, M., Milivojević, M., Bojić, L., Erceg, S., Stevanović, M.,& Švirtlih, M.. (2023). Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society Belgrade : Serbian Neuroscience Society., 96-96. https://hdl.handle.net/21.15107/rcub_imagine_2287
Lazić S, Stanisavljević Ninković D, Petrović I, Aleksandra M, Milivojević M, Bojić L, Erceg S, Stevanović M, Švirtlih M. Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society. 2023;:96-96. https://hdl.handle.net/21.15107/rcub_imagine_2287 .
Lazić, Stefan, Stanisavljević Ninković, Danijela, Petrović, Isidora, Aleksandra, Medić, Milivojević, Milena, Bojić, Luka, Erceg, Slaven, Stevanović, Milena, Švirtlih, Marija, "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21" in 8th Congress of the Serbian Neuroscience Society (2023):96-96, https://hdl.handle.net/21.15107/rcub_imagine_2287 .