Expression of Dense-core Vesicles and of Their Exocytosis Are Governed by the Repressive Transcription Factor NRSF/REST
Само за регистроване кориснике
2009
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The mechanism by which neurons and neurosecretory cells govern the expression and the exocytic discharge of their clear and dense-core vesicles had remained unclear until recently when studies in the neurosecretory cell model PC12 revealed these processes to be orchestrated by the transcriptional repressor neuron restrictive silencer factor (NRSF)/repressor element-1 silencing transcription factor (REST). In wild-type PC12 fully competent for neurosecretion, NRSF/REST is low. The genes of the proteins involved in neurosecretion [from the secretory to vesicle membrane and plasma membrane proteins, including the soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) of exocytosis] were all repressed by increases of NRSF/REST expression to various extents when the increase was only a fewfold but were completely or almost completely repressed when the increase was large, as in spontaneously defective PC 12 clones. In the first case the dense-core vesicles were stil...l competent for exocytosis but were smaller and less dense than in wild-type cells; in the second they were no longer visible but did reappear when the repression was attenuated by transfection of a dominant-negative construct of NRSF/REST combined with a secretory chromogranin or strengthened by treatment with a blocker of NRSF/REST-associated enzymes, the histone deacetylases.
Кључне речи:
transcription / SNARE / PC12 / histone deacetylase / chromograninИзвор:
Mechanisms of Exocytosis, 2009, 1152, 194-200Издавач:
- Wiley-Blackwell, Malden
Финансирање / пројекти:
- European Community [APOPIS-LSHM-CT-2003-503330]
- Telethon Fondazione Onlus [GGGP030234]
- Italian Ministry of Research
DOI: 10.1111/j.1749-6632.2008.03988.x
ISSN: 0077-8923
PubMed: 19161390
WoS: 000263265600020
Scopus: 2-s2.0-58549116920
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - D'Alessandro, Rosalba AU - Lazić, Andrijana AU - Meldolesi, Jacopo PY - 2009 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/365 AB - The mechanism by which neurons and neurosecretory cells govern the expression and the exocytic discharge of their clear and dense-core vesicles had remained unclear until recently when studies in the neurosecretory cell model PC12 revealed these processes to be orchestrated by the transcriptional repressor neuron restrictive silencer factor (NRSF)/repressor element-1 silencing transcription factor (REST). In wild-type PC12 fully competent for neurosecretion, NRSF/REST is low. The genes of the proteins involved in neurosecretion [from the secretory to vesicle membrane and plasma membrane proteins, including the soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) of exocytosis] were all repressed by increases of NRSF/REST expression to various extents when the increase was only a fewfold but were completely or almost completely repressed when the increase was large, as in spontaneously defective PC 12 clones. In the first case the dense-core vesicles were still competent for exocytosis but were smaller and less dense than in wild-type cells; in the second they were no longer visible but did reappear when the repression was attenuated by transfection of a dominant-negative construct of NRSF/REST combined with a secretory chromogranin or strengthened by treatment with a blocker of NRSF/REST-associated enzymes, the histone deacetylases. PB - Wiley-Blackwell, Malden T2 - Mechanisms of Exocytosis T1 - Expression of Dense-core Vesicles and of Their Exocytosis Are Governed by the Repressive Transcription Factor NRSF/REST EP - 200 SP - 194 VL - 1152 DO - 10.1111/j.1749-6632.2008.03988.x ER -
@article{ author = "D'Alessandro, Rosalba and Lazić, Andrijana and Meldolesi, Jacopo", year = "2009", abstract = "The mechanism by which neurons and neurosecretory cells govern the expression and the exocytic discharge of their clear and dense-core vesicles had remained unclear until recently when studies in the neurosecretory cell model PC12 revealed these processes to be orchestrated by the transcriptional repressor neuron restrictive silencer factor (NRSF)/repressor element-1 silencing transcription factor (REST). In wild-type PC12 fully competent for neurosecretion, NRSF/REST is low. The genes of the proteins involved in neurosecretion [from the secretory to vesicle membrane and plasma membrane proteins, including the soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) of exocytosis] were all repressed by increases of NRSF/REST expression to various extents when the increase was only a fewfold but were completely or almost completely repressed when the increase was large, as in spontaneously defective PC 12 clones. In the first case the dense-core vesicles were still competent for exocytosis but were smaller and less dense than in wild-type cells; in the second they were no longer visible but did reappear when the repression was attenuated by transfection of a dominant-negative construct of NRSF/REST combined with a secretory chromogranin or strengthened by treatment with a blocker of NRSF/REST-associated enzymes, the histone deacetylases.", publisher = "Wiley-Blackwell, Malden", journal = "Mechanisms of Exocytosis", title = "Expression of Dense-core Vesicles and of Their Exocytosis Are Governed by the Repressive Transcription Factor NRSF/REST", pages = "200-194", volume = "1152", doi = "10.1111/j.1749-6632.2008.03988.x" }
D'Alessandro, R., Lazić, A.,& Meldolesi, J.. (2009). Expression of Dense-core Vesicles and of Their Exocytosis Are Governed by the Repressive Transcription Factor NRSF/REST. in Mechanisms of Exocytosis Wiley-Blackwell, Malden., 1152, 194-200. https://doi.org/10.1111/j.1749-6632.2008.03988.x
D'Alessandro R, Lazić A, Meldolesi J. Expression of Dense-core Vesicles and of Their Exocytosis Are Governed by the Repressive Transcription Factor NRSF/REST. in Mechanisms of Exocytosis. 2009;1152:194-200. doi:10.1111/j.1749-6632.2008.03988.x .
D'Alessandro, Rosalba, Lazić, Andrijana, Meldolesi, Jacopo, "Expression of Dense-core Vesicles and of Their Exocytosis Are Governed by the Repressive Transcription Factor NRSF/REST" in Mechanisms of Exocytosis, 1152 (2009):194-200, https://doi.org/10.1111/j.1749-6632.2008.03988.x . .