Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench)
Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench)
2010
Аутори
Timotijević, GordanaMilisavljević, Mira
Radović, Svetlana R.
Konstantinović, M.M.
Maksimović, Vesna R.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Gen za aspartičnu proteinazu (FeAP12) je izolovan iz eDNA biblioteke semena heljde u razviću. Analiza izvedene amino kiselinske sekvence FeAP12 gena ukazuje na njenu visoku homologiju sa ostalim tipičnim biljnim aspartičnim proteinazama (AP) koje se odlikuju prisustvom biljno specifičnog inserta (plant specific insert PSI), jedinstvenog među AP. Pokazano je da gen FeAP12 nije eksprimiran u listu, korenu, stablu i cvetu, već da je iRNA za FeAP12 prisutna samo u semenu. Najveći nivo ekspresije ovog gena je uočen u ranim fazama razvića semena, što ukazuje na njegovu moguću ulogu u degradaciji nucelusa.
Aspartic proteinase gene (FeAP12) has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP) characterized by the presence of a plant-specific insert (PSI), unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.
Кључне речи:
gene expression / cDNA / buckwheat / Aspartic proteinaseИзвор:
Archives of Biological Sciences, 2010, 62, 1, 143-151Издавач:
- Srpsko biološko društvo, Beograd, i dr.
Финансирање / пројекти:
- Структура, функција и регулација експресије одабраних биљних гена (RS-MESTD-MPN2006-2010-143017)
DOI: 10.2298/ABS1001143T
ISSN: 0354-4664
WoS: 000277610000019
Scopus: 2-s2.0-77955132922
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Timotijević, Gordana AU - Milisavljević, Mira AU - Radović, Svetlana R. AU - Konstantinović, M.M. AU - Maksimović, Vesna R. PY - 2010 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/429 AB - Gen za aspartičnu proteinazu (FeAP12) je izolovan iz eDNA biblioteke semena heljde u razviću. Analiza izvedene amino kiselinske sekvence FeAP12 gena ukazuje na njenu visoku homologiju sa ostalim tipičnim biljnim aspartičnim proteinazama (AP) koje se odlikuju prisustvom biljno specifičnog inserta (plant specific insert PSI), jedinstvenog među AP. Pokazano je da gen FeAP12 nije eksprimiran u listu, korenu, stablu i cvetu, već da je iRNA za FeAP12 prisutna samo u semenu. Najveći nivo ekspresije ovog gena je uočen u ranim fazama razvića semena, što ukazuje na njegovu moguću ulogu u degradaciji nucelusa. AB - Aspartic proteinase gene (FeAP12) has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP) characterized by the presence of a plant-specific insert (PSI), unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench) T1 - Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench) EP - 151 IS - 1 SP - 143 VL - 62 DO - 10.2298/ABS1001143T ER -
@article{ author = "Timotijević, Gordana and Milisavljević, Mira and Radović, Svetlana R. and Konstantinović, M.M. and Maksimović, Vesna R.", year = "2010", abstract = "Gen za aspartičnu proteinazu (FeAP12) je izolovan iz eDNA biblioteke semena heljde u razviću. Analiza izvedene amino kiselinske sekvence FeAP12 gena ukazuje na njenu visoku homologiju sa ostalim tipičnim biljnim aspartičnim proteinazama (AP) koje se odlikuju prisustvom biljno specifičnog inserta (plant specific insert PSI), jedinstvenog među AP. Pokazano je da gen FeAP12 nije eksprimiran u listu, korenu, stablu i cvetu, već da je iRNA za FeAP12 prisutna samo u semenu. Najveći nivo ekspresije ovog gena je uočen u ranim fazama razvića semena, što ukazuje na njegovu moguću ulogu u degradaciji nucelusa., Aspartic proteinase gene (FeAP12) has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP) characterized by the presence of a plant-specific insert (PSI), unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench), Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench)", pages = "151-143", number = "1", volume = "62", doi = "10.2298/ABS1001143T" }
Timotijević, G., Milisavljević, M., Radović, S. R., Konstantinović, M.M.,& Maksimović, V. R.. (2010). Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench). in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 62(1), 143-151. https://doi.org/10.2298/ABS1001143T
Timotijević G, Milisavljević M, Radović SR, Konstantinović M, Maksimović VR. Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench). in Archives of Biological Sciences. 2010;62(1):143-151. doi:10.2298/ABS1001143T .
Timotijević, Gordana, Milisavljević, Mira, Radović, Svetlana R., Konstantinović, M.M., Maksimović, Vesna R., "Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench)" in Archives of Biological Sciences, 62, no. 1 (2010):143-151, https://doi.org/10.2298/ABS1001143T . .