Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience
Само за регистроване кориснике
2015
Аутори
Srzentić Dražilov, SanjaNikčević, Gordana
Spasovski, Dusko
Bascarević, Zoran
Zivković, Zorica
Terzić-Supić, Zorica
Matanović, Dragana
Đorđević, Valentina
Pavlović, Sonja
Spasovski, Vesna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Perthes disease is one of the most common forms of pediatric femoral head osteonecrosis with an unknown etiology. Coagulation factors were the first genetic factors suspected to have a role in the pathogenesis of this disease, but studies showed inconsistent results. It is described that inflammation is present during early stages of Perthes disease, but its genetic aspect has not been studied extensively. Little is known regarding the status of apoptotic factors during the repair process that leads to the occurrence of hip deformity in patients. Therefore, the aim of this study was to analyze major mediators involved in coagulation, inflammation, and apoptotic processes as possible causative factors of Perthes disease. The study cohort consisted of 37 patients. Gene variants of TNF-alpha, FV, FII, and MTHFR genes were determined by PCR-RFLP, while IL-3 and PAI-1 were genotyped by direct sequencing. The expression level of Bax, Bcl-2, Bcl2L12, Fas and FasL was analyzed by quantitative ...reverse-transcriptase polymerase chain reaction (qRT-PCR) technique. Our results showed a significantly increased level of expression of pro-apoptotic factor Bax along with significantly higher Bax/Bcl-2 ratio in the patient group. Conclusion: The results presented indicate that apoptosis could be one of the factors contributing to the lack of balanced bone remodeling process in Perthes patients.
Кључне речи:
Perthes disease / Inflammation / Bone remodeling / Blood coagulation factors / ApoptosisИзвор:
European Journal of Pediatrics, 2015, 174, 8, 1085-1092Издавач:
- Springer, New York
Финансирање / пројекти:
- Ретке болести: молекуларна патофизиологија, дијагностички и терапијски модалитети и социјални, етички и правни аспекти (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41004)
DOI: 10.1007/s00431-015-2510-z
ISSN: 0340-6199
PubMed: 25754626
WoS: 000358650500012
Scopus: 2-s2.0-84938287430
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Srzentić Dražilov, Sanja AU - Nikčević, Gordana AU - Spasovski, Dusko AU - Bascarević, Zoran AU - Zivković, Zorica AU - Terzić-Supić, Zorica AU - Matanović, Dragana AU - Đorđević, Valentina AU - Pavlović, Sonja AU - Spasovski, Vesna PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/796 AB - Perthes disease is one of the most common forms of pediatric femoral head osteonecrosis with an unknown etiology. Coagulation factors were the first genetic factors suspected to have a role in the pathogenesis of this disease, but studies showed inconsistent results. It is described that inflammation is present during early stages of Perthes disease, but its genetic aspect has not been studied extensively. Little is known regarding the status of apoptotic factors during the repair process that leads to the occurrence of hip deformity in patients. Therefore, the aim of this study was to analyze major mediators involved in coagulation, inflammation, and apoptotic processes as possible causative factors of Perthes disease. The study cohort consisted of 37 patients. Gene variants of TNF-alpha, FV, FII, and MTHFR genes were determined by PCR-RFLP, while IL-3 and PAI-1 were genotyped by direct sequencing. The expression level of Bax, Bcl-2, Bcl2L12, Fas and FasL was analyzed by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) technique. Our results showed a significantly increased level of expression of pro-apoptotic factor Bax along with significantly higher Bax/Bcl-2 ratio in the patient group. Conclusion: The results presented indicate that apoptosis could be one of the factors contributing to the lack of balanced bone remodeling process in Perthes patients. PB - Springer, New York T2 - European Journal of Pediatrics T1 - Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience EP - 1092 IS - 8 SP - 1085 VL - 174 DO - 10.1007/s00431-015-2510-z ER -
@article{ author = "Srzentić Dražilov, Sanja and Nikčević, Gordana and Spasovski, Dusko and Bascarević, Zoran and Zivković, Zorica and Terzić-Supić, Zorica and Matanović, Dragana and Đorđević, Valentina and Pavlović, Sonja and Spasovski, Vesna", year = "2015", abstract = "Perthes disease is one of the most common forms of pediatric femoral head osteonecrosis with an unknown etiology. Coagulation factors were the first genetic factors suspected to have a role in the pathogenesis of this disease, but studies showed inconsistent results. It is described that inflammation is present during early stages of Perthes disease, but its genetic aspect has not been studied extensively. Little is known regarding the status of apoptotic factors during the repair process that leads to the occurrence of hip deformity in patients. Therefore, the aim of this study was to analyze major mediators involved in coagulation, inflammation, and apoptotic processes as possible causative factors of Perthes disease. The study cohort consisted of 37 patients. Gene variants of TNF-alpha, FV, FII, and MTHFR genes were determined by PCR-RFLP, while IL-3 and PAI-1 were genotyped by direct sequencing. The expression level of Bax, Bcl-2, Bcl2L12, Fas and FasL was analyzed by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) technique. Our results showed a significantly increased level of expression of pro-apoptotic factor Bax along with significantly higher Bax/Bcl-2 ratio in the patient group. Conclusion: The results presented indicate that apoptosis could be one of the factors contributing to the lack of balanced bone remodeling process in Perthes patients.", publisher = "Springer, New York", journal = "European Journal of Pediatrics", title = "Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience", pages = "1092-1085", number = "8", volume = "174", doi = "10.1007/s00431-015-2510-z" }
Srzentić Dražilov, S., Nikčević, G., Spasovski, D., Bascarević, Z., Zivković, Z., Terzić-Supić, Z., Matanović, D., Đorđević, V., Pavlović, S.,& Spasovski, V.. (2015). Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience. in European Journal of Pediatrics Springer, New York., 174(8), 1085-1092. https://doi.org/10.1007/s00431-015-2510-z
Srzentić Dražilov S, Nikčević G, Spasovski D, Bascarević Z, Zivković Z, Terzić-Supić Z, Matanović D, Đorđević V, Pavlović S, Spasovski V. Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience. in European Journal of Pediatrics. 2015;174(8):1085-1092. doi:10.1007/s00431-015-2510-z .
Srzentić Dražilov, Sanja, Nikčević, Gordana, Spasovski, Dusko, Bascarević, Zoran, Zivković, Zorica, Terzić-Supić, Zorica, Matanović, Dragana, Đorđević, Valentina, Pavlović, Sonja, Spasovski, Vesna, "Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience" in European Journal of Pediatrics, 174, no. 8 (2015):1085-1092, https://doi.org/10.1007/s00431-015-2510-z . .