Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction
Само за регистроване кориснике
2016
Аутори
Rakocević, JelenaKojić, Snežana
Orlić, Dejan
Stanković, Goran
Ostojić, Miodrag
Petrović, Olga
Zaletel, Ivan
Puskas, Nela
Todorović, Vera
Labudović-Borović, Milica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: Angiogenesis is the growth of both new vascular and lymphatic blood vessels from the existing vasculature. During this process, blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) express specific markers, which help their discrimination and easier identification. Since the coronary thrombi material aspirated from patients with ST-elevation myocardial infarction (STEMI) proved as good angiogenesis model, we investigated the expression of CD34 and CD31 as BECs markers, and D2-40, LYVE-1 and VEGFR3 as LEC markers in this material. Materials and methods: Aspirated thrombi were stained immunohistochemically for CD34, CD31, D2-40, LYVE-1 and VEGFR3. Organizational patterns of immunopositive cells were graded as single cells, clusters or microvessels. Double immunofluorescence for CD31, D2-40, LYVE-1 and VEGRF3 was done. Thrombi were also graded as fresh ( lt 1 day old), lytic (1-5 days old) and organized ( gt 5 days old). Results: Serial sections of aspirated... thrombi showed concordant BEC and LEC markers immunopositivity. Double immunoflorescence proved co-expression of CD31 and LEC markers on the same cells. Cells expressing LEC markers organized in clusters and microvessels were mainly present in lytic and organized thrombi. Conclusion: Co-expression of BEC and LEC markers on the same non-tumorous cell during thrombus neovascularization indicates existing in vivo plasticity of endothelial cells under non-tumorous pathological conditions. It also points that CD34 and CD31 on one hand, and D2-40, LYVE-1 and VEGFR3 immunostaining on the other hand, cannot solely be a reliable indicators whether vessel is lymphatic or not.
Кључне речи:
VEGFR3 / ST-elevation myocardial infarction / LYVE-1 / D2-40 / Coronary thrombus neovascularization / Coronary thrombus aspiration / CD34 / CD31Извор:
Experimental and Molecular Pathology, 2016, 100, 1, 31-38Издавач:
- Academic Press Inc Elsevier Science, San Diego
Финансирање / пројекти:
- U.S. Embassy in Belgrade [SRB100-15-GR-355]
- Акутни коронарни синдром: истраживање вулнерабилности (плака, крви и миокарда), оптимално лечење и одређивање прогностичких фактора (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41022)
- Антиоксидативна заштита и потенцијали за диференцијацију и регенерацију мезенхималних матичних ћелија из различитих ткива током процеса старења (RS-MESTD-Basic Research (BR or ON)-175061)
- Каротидна болест у Србији - патолошка динамика, превенција, дијагностика и иновативни терапијски поступци (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41002)
- Ћелијске и молекулске основе малигних и кардиоваскуларних обољења-клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41027)
DOI: 10.1016/j.yexmp.2015.11.028
ISSN: 0014-4800
PubMed: 26657000
WoS: 000369879100006
Scopus: 2-s2.0-84949591464
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Rakocević, Jelena AU - Kojić, Snežana AU - Orlić, Dejan AU - Stanković, Goran AU - Ostojić, Miodrag AU - Petrović, Olga AU - Zaletel, Ivan AU - Puskas, Nela AU - Todorović, Vera AU - Labudović-Borović, Milica PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/979 AB - Introduction: Angiogenesis is the growth of both new vascular and lymphatic blood vessels from the existing vasculature. During this process, blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) express specific markers, which help their discrimination and easier identification. Since the coronary thrombi material aspirated from patients with ST-elevation myocardial infarction (STEMI) proved as good angiogenesis model, we investigated the expression of CD34 and CD31 as BECs markers, and D2-40, LYVE-1 and VEGFR3 as LEC markers in this material. Materials and methods: Aspirated thrombi were stained immunohistochemically for CD34, CD31, D2-40, LYVE-1 and VEGFR3. Organizational patterns of immunopositive cells were graded as single cells, clusters or microvessels. Double immunofluorescence for CD31, D2-40, LYVE-1 and VEGRF3 was done. Thrombi were also graded as fresh ( lt 1 day old), lytic (1-5 days old) and organized ( gt 5 days old). Results: Serial sections of aspirated thrombi showed concordant BEC and LEC markers immunopositivity. Double immunoflorescence proved co-expression of CD31 and LEC markers on the same cells. Cells expressing LEC markers organized in clusters and microvessels were mainly present in lytic and organized thrombi. Conclusion: Co-expression of BEC and LEC markers on the same non-tumorous cell during thrombus neovascularization indicates existing in vivo plasticity of endothelial cells under non-tumorous pathological conditions. It also points that CD34 and CD31 on one hand, and D2-40, LYVE-1 and VEGFR3 immunostaining on the other hand, cannot solely be a reliable indicators whether vessel is lymphatic or not. PB - Academic Press Inc Elsevier Science, San Diego T2 - Experimental and Molecular Pathology T1 - Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction EP - 38 IS - 1 SP - 31 VL - 100 DO - 10.1016/j.yexmp.2015.11.028 ER -
@article{ author = "Rakocević, Jelena and Kojić, Snežana and Orlić, Dejan and Stanković, Goran and Ostojić, Miodrag and Petrović, Olga and Zaletel, Ivan and Puskas, Nela and Todorović, Vera and Labudović-Borović, Milica", year = "2016", abstract = "Introduction: Angiogenesis is the growth of both new vascular and lymphatic blood vessels from the existing vasculature. During this process, blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) express specific markers, which help their discrimination and easier identification. Since the coronary thrombi material aspirated from patients with ST-elevation myocardial infarction (STEMI) proved as good angiogenesis model, we investigated the expression of CD34 and CD31 as BECs markers, and D2-40, LYVE-1 and VEGFR3 as LEC markers in this material. Materials and methods: Aspirated thrombi were stained immunohistochemically for CD34, CD31, D2-40, LYVE-1 and VEGFR3. Organizational patterns of immunopositive cells were graded as single cells, clusters or microvessels. Double immunofluorescence for CD31, D2-40, LYVE-1 and VEGRF3 was done. Thrombi were also graded as fresh ( lt 1 day old), lytic (1-5 days old) and organized ( gt 5 days old). Results: Serial sections of aspirated thrombi showed concordant BEC and LEC markers immunopositivity. Double immunoflorescence proved co-expression of CD31 and LEC markers on the same cells. Cells expressing LEC markers organized in clusters and microvessels were mainly present in lytic and organized thrombi. Conclusion: Co-expression of BEC and LEC markers on the same non-tumorous cell during thrombus neovascularization indicates existing in vivo plasticity of endothelial cells under non-tumorous pathological conditions. It also points that CD34 and CD31 on one hand, and D2-40, LYVE-1 and VEGFR3 immunostaining on the other hand, cannot solely be a reliable indicators whether vessel is lymphatic or not.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Experimental and Molecular Pathology", title = "Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction", pages = "38-31", number = "1", volume = "100", doi = "10.1016/j.yexmp.2015.11.028" }
Rakocević, J., Kojić, S., Orlić, D., Stanković, G., Ostojić, M., Petrović, O., Zaletel, I., Puskas, N., Todorović, V.,& Labudović-Borović, M.. (2016). Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction. in Experimental and Molecular Pathology Academic Press Inc Elsevier Science, San Diego., 100(1), 31-38. https://doi.org/10.1016/j.yexmp.2015.11.028
Rakocević J, Kojić S, Orlić D, Stanković G, Ostojić M, Petrović O, Zaletel I, Puskas N, Todorović V, Labudović-Borović M. Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction. in Experimental and Molecular Pathology. 2016;100(1):31-38. doi:10.1016/j.yexmp.2015.11.028 .
Rakocević, Jelena, Kojić, Snežana, Orlić, Dejan, Stanković, Goran, Ostojić, Miodrag, Petrović, Olga, Zaletel, Ivan, Puskas, Nela, Todorović, Vera, Labudović-Borović, Milica, "Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction" in Experimental and Molecular Pathology, 100, no. 1 (2016):31-38, https://doi.org/10.1016/j.yexmp.2015.11.028 . .