TY  - CONF
AU  - Dinić, Miroslav
AU  - L. Burgess, Jamie
AU  - Lukić, Jovanka
AU  - Catanuto, Paola
AU  - Radojević, Dušan
AU  - Marjanović, Jelena
AU  - Verpile, Rebecca
AU  - R. Thaller, Seth
AU  - Gonzalez, Tammy
AU  - Golić, Nataša
AU  - Tomić- Canić, Marjana
AU  - Strahinić, Ivana
AU  - Pastar, Irena
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2378
AB  - Skin microbiome emerged as an important
factor which can balance tissue repair process
and wound healing. Recent evidence suggest
that intracellular bacterial localization could be
associated with the aberrant healing observed
in patients with chronic wounds, while therapeutics
targeting intracellular bacteria remain
limited. Probiotic lactobacilli and their bioactive
lysates (postbiotics) are well known for their role
in maintenance of gut epithelial homeostasis.
Hence, in this study we focused to understand
the mechanisms of cutaneous response to fourteen
postbiotics derived from different lactobacilli
to reduce intracellular Staphylococcus aureus
colonization and promote healing. Latilactobacillus
curvatus BGMK2-41 demonstrated the
most efficient capability to reduce intracellular infection by S. aureus in keratinocytes in vitro and
infection of human skin explants. Reduction of
bacterial number was followed by upregulation
of the expression of antimicrobial response
genes. Furthermore, BGMK2-41 postbiotic treatment
stimulates keratinocyte migration in vitro
and increases expression of anti-inflammatory
cytokine IL-10, promotes wound closure and
strengthens the epidermal barrier via upregulation
of tight junction proteins in a human ex vivo
wound model. Altogether, this study provided
evidence that postbiotics could stimulate fortification
of epithelial barrier to suppress dissemination
of intracellular pathogens which can be
used as a novel approach to treat dermatologic
and wound healing disorders associated with
persistent infections.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING
EP  - 133
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2378
ER  - 

@conference{
author = "Dinić, Miroslav and L. Burgess, Jamie and Lukić, Jovanka and Catanuto, Paola and Radojević, Dušan and Marjanović, Jelena and Verpile, Rebecca and R. Thaller, Seth and Gonzalez, Tammy and Golić, Nataša and Tomić- Canić, Marjana and Strahinić, Ivana and Pastar, Irena",
year = "2024",
abstract = "Skin microbiome emerged as an important
factor which can balance tissue repair process
and wound healing. Recent evidence suggest
that intracellular bacterial localization could be
associated with the aberrant healing observed
in patients with chronic wounds, while therapeutics
targeting intracellular bacteria remain
limited. Probiotic lactobacilli and their bioactive
lysates (postbiotics) are well known for their role
in maintenance of gut epithelial homeostasis.
Hence, in this study we focused to understand
the mechanisms of cutaneous response to fourteen
postbiotics derived from different lactobacilli
to reduce intracellular Staphylococcus aureus
colonization and promote healing. Latilactobacillus
curvatus BGMK2-41 demonstrated the
most efficient capability to reduce intracellular infection by S. aureus in keratinocytes in vitro and
infection of human skin explants. Reduction of
bacterial number was followed by upregulation
of the expression of antimicrobial response
genes. Furthermore, BGMK2-41 postbiotic treatment
stimulates keratinocyte migration in vitro
and increases expression of anti-inflammatory
cytokine IL-10, promotes wound closure and
strengthens the epidermal barrier via upregulation
of tight junction proteins in a human ex vivo
wound model. Altogether, this study provided
evidence that postbiotics could stimulate fortification
of epithelial barrier to suppress dissemination
of intracellular pathogens which can be
used as a novel approach to treat dermatologic
and wound healing disorders associated with
persistent infections.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2378"
}

Dinić, M., L. Burgess, J., Lukić, J., Catanuto, P., Radojević, D., Marjanović, J., Verpile, R., R. Thaller, S., Gonzalez, T., Golić, N., Tomić- Canić, M., Strahinić, I.,& Pastar, I.. (2024). HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 133-133.
https://hdl.handle.net/21.15107/rcub_imagine_2378

Dinić M, L. Burgess J, Lukić J, Catanuto P, Radojević D, Marjanović J, Verpile R, R. Thaller S, Gonzalez T, Golić N, Tomić- Canić M, Strahinić I, Pastar I. HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:133-133.
https://hdl.handle.net/21.15107/rcub_imagine_2378 .

Dinić, Miroslav, L. Burgess, Jamie, Lukić, Jovanka, Catanuto, Paola, Radojević, Dušan, Marjanović, Jelena, Verpile, Rebecca, R. Thaller, Seth, Gonzalez, Tammy, Golić, Nataša, Tomić- Canić, Marjana, Strahinić, Ivana, Pastar, Irena, "HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):133-133,
https://hdl.handle.net/21.15107/rcub_imagine_2378 .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Strahinić, Ivana
AU  - Milenković, Marina
AU  - Begović, Jelena
PY  - 2023
UR  - https://aseestant.ceon.rs/index.php/arhfarm/article/view/46399
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2298
AB  - The aim of the study was to test the potential of immunostimulating Limosilactobacillus fermentum BGHI14 strain to reduce the damage of colon tissue in rats with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. Wistar rats were treated with L. fermentum BGHI14 in the regime of preventive, therapeutic and continuous treatment 22 days prior to and/or 7 days after the administration of TNBS. After sacrifice, the colon tissue samples were taken for RNA isolation, gene expression analysis, histopathological analysis, and malondialdehyde measurement. Judging from the body weights, histopathological scores, malondialdehyde levels and transcription of IL-1β and Tight junction protein 1 (Tjp-1) coding genes, preventive and therapeutic treatment proved to be the most protective in the applied conditions. On the other hand, continuous treatment did not affect the intensity of tissue damage. Considering these results, we discussed the possible mechanisms which might stand behind the protective action of immunostimulating probiotic bacteria in the case of mucosal barrier damage.
AB  - Cilj  ovog  istraživanja  je  bio  da  se  ispita  potencijal  imunostimulišućeg  soja Limosilactobacillus fermentumBGHI14 da smanji oštećenje tkiva debelog creva kod pacova sa kolitisom  izazvanim  2,4,6-trinitrobenzensulfonskom  kiselinom  (TNBS).  Pacovi  Wistar  soja  su  tretirani  sojem  L.  fermentumBGHI14  u  režimu  preventivnog,  terapijskog  i  kontinuiranog  tretmana 22 dana pre i/ili 7 dana nakon primene TNBS. Nakon žrtvovanja, uzorci debelog creva su  sakupljeni  za  izolaciju  RNK,  analizu  ekspresije  gena,  histopatološke  analize  i  merenje  malondialdehida.    Na    osnovu    telesnih    težina    pacova,    histopatološih    rezultata,    nivoa    malondialdehida  i  transkripcije  IL-1β  citokina  i  proteina  tesnih  međućelijskih  veza  (Tjp-1), preventivni i terapijski tretman su se pokazali kao najefikasniji u primenjenim uslovima. S druge strane, kontinuirano lečenje nije uticalo na intenzitet oštećenja tkiva. Uzimajući u obzir ove rezultate, razmotreni su mogući mehanizmi koji stoje iza zaštitnog delovanja imunostimulišućih probiotičkih bakterija u slučaju oštećenja mukozne barijere.
PB  - Centre for Evaluation in Education and Science
T2  - Archives of Pharmacy
T1  - Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis
T1  - Efekat imunostimulišućeg soja roda Limosilactobacillus kod pacova sa kolitisom izazvanim trinitrobenzensulfonatom (TNBS)
EP  - 601
IS  - Notebook 6
SP  - 586
VL  - 73
DO  - 10.5937/arhfarm73-46399
ER  - 

@article{
author = "Lukić, Jovanka and Strahinić, Ivana and Milenković, Marina and Begović, Jelena",
year = "2023",
abstract = "The aim of the study was to test the potential of immunostimulating Limosilactobacillus fermentum BGHI14 strain to reduce the damage of colon tissue in rats with 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis. Wistar rats were treated with L. fermentum BGHI14 in the regime of preventive, therapeutic and continuous treatment 22 days prior to and/or 7 days after the administration of TNBS. After sacrifice, the colon tissue samples were taken for RNA isolation, gene expression analysis, histopathological analysis, and malondialdehyde measurement. Judging from the body weights, histopathological scores, malondialdehyde levels and transcription of IL-1β and Tight junction protein 1 (Tjp-1) coding genes, preventive and therapeutic treatment proved to be the most protective in the applied conditions. On the other hand, continuous treatment did not affect the intensity of tissue damage. Considering these results, we discussed the possible mechanisms which might stand behind the protective action of immunostimulating probiotic bacteria in the case of mucosal barrier damage., Cilj  ovog  istraživanja  je  bio  da  se  ispita  potencijal  imunostimulišućeg  soja Limosilactobacillus fermentumBGHI14 da smanji oštećenje tkiva debelog creva kod pacova sa kolitisom  izazvanim  2,4,6-trinitrobenzensulfonskom  kiselinom  (TNBS).  Pacovi  Wistar  soja  su  tretirani  sojem  L.  fermentumBGHI14  u  režimu  preventivnog,  terapijskog  i  kontinuiranog  tretmana 22 dana pre i/ili 7 dana nakon primene TNBS. Nakon žrtvovanja, uzorci debelog creva su  sakupljeni  za  izolaciju  RNK,  analizu  ekspresije  gena,  histopatološke  analize  i  merenje  malondialdehida.    Na    osnovu    telesnih    težina    pacova,    histopatološih    rezultata,    nivoa    malondialdehida  i  transkripcije  IL-1β  citokina  i  proteina  tesnih  međućelijskih  veza  (Tjp-1), preventivni i terapijski tretman su se pokazali kao najefikasniji u primenjenim uslovima. S druge strane, kontinuirano lečenje nije uticalo na intenzitet oštećenja tkiva. Uzimajući u obzir ove rezultate, razmotreni su mogući mehanizmi koji stoje iza zaštitnog delovanja imunostimulišućih probiotičkih bakterija u slučaju oštećenja mukozne barijere.",
publisher = "Centre for Evaluation in Education and Science",
journal = "Archives of Pharmacy",
title = "Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis, Efekat imunostimulišućeg soja roda Limosilactobacillus kod pacova sa kolitisom izazvanim trinitrobenzensulfonatom (TNBS)",
pages = "601-586",
number = "Notebook 6",
volume = "73",
doi = "10.5937/arhfarm73-46399"
}

Lukić, J., Strahinić, I., Milenković, M.,& Begović, J.. (2023). Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis. in Archives of Pharmacy
Centre for Evaluation in Education and Science., 73(Notebook 6), 586-601.
https://doi.org/10.5937/arhfarm73-46399

Lukić J, Strahinić I, Milenković M, Begović J. Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis. in Archives of Pharmacy. 2023;73(Notebook 6):586-601.
doi:10.5937/arhfarm73-46399 .

Lukić, Jovanka, Strahinić, Ivana, Milenković, Marina, Begović, Jelena, "Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis" in Archives of Pharmacy, 73, no. Notebook 6 (2023):586-601,
https://doi.org/10.5937/arhfarm73-46399 . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Jakovljević, Stefan
AU  - Đokić, Jelena
AU  - Popović, Nikola
AU  - Radojević, Dušan
AU  - Strahinić, Ivana
AU  - Golić, Nataša
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1473
AB  - The host-microbiota cross-talk represents an important factor contributing to innate immune response and host resistance during infection. It has been shown that probiotic lactobacilli exhibit the ability to modulate innate immunity and enhance pathogen elimination. Here we showed that heat-inactivated probiotic strain Lactobacillus curvatus BGMK2-41 stimulates immune response and resistance of the Caenorhabditis elegans against Staphylococcus aureus and Pseudomonas aeruginosa. By employing qRT-PCR and western blot analysis we showed that heat-inactivated BGMK2-41 activated PMK-1/p38 MAPK immunity pathway which prolongs the survival of C. elegans exposed to pathogenic bacteria in nematode killing assays. The C. elegans pmk-1 mutant was used to demonstrate a mechanistic basis for the antimicrobial potential of BGMK2-41, showing that BGMK2-41 upregulated PMK-1/p38 MAPK dependent transcription of C-type lectins, lysozymes and tight junction protein CLC-1. Overall, this study suggests that PMK-1/p38 MAPK-dependent immune regulation by BGMK2-41 is essential for probiotic-mediated C. elegans protection against gram-positive and gram-negative bacteria and could be further explored for development of probiotics with the potential to increase resistance of the host towards pathogens.
PB  - Nature Portfolio, Berlin
T2  - Scientific Reports
T1  - Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria
IS  - 1
VL  - 11
DO  - 10.1038/s41598-021-00698-5
ER  - 

@article{
author = "Dinić, Miroslav and Jakovljević, Stefan and Đokić, Jelena and Popović, Nikola and Radojević, Dušan and Strahinić, Ivana and Golić, Nataša",
year = "2021",
abstract = "The host-microbiota cross-talk represents an important factor contributing to innate immune response and host resistance during infection. It has been shown that probiotic lactobacilli exhibit the ability to modulate innate immunity and enhance pathogen elimination. Here we showed that heat-inactivated probiotic strain Lactobacillus curvatus BGMK2-41 stimulates immune response and resistance of the Caenorhabditis elegans against Staphylococcus aureus and Pseudomonas aeruginosa. By employing qRT-PCR and western blot analysis we showed that heat-inactivated BGMK2-41 activated PMK-1/p38 MAPK immunity pathway which prolongs the survival of C. elegans exposed to pathogenic bacteria in nematode killing assays. The C. elegans pmk-1 mutant was used to demonstrate a mechanistic basis for the antimicrobial potential of BGMK2-41, showing that BGMK2-41 upregulated PMK-1/p38 MAPK dependent transcription of C-type lectins, lysozymes and tight junction protein CLC-1. Overall, this study suggests that PMK-1/p38 MAPK-dependent immune regulation by BGMK2-41 is essential for probiotic-mediated C. elegans protection against gram-positive and gram-negative bacteria and could be further explored for development of probiotics with the potential to increase resistance of the host towards pathogens.",
publisher = "Nature Portfolio, Berlin",
journal = "Scientific Reports",
title = "Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria",
number = "1",
volume = "11",
doi = "10.1038/s41598-021-00698-5"
}

Dinić, M., Jakovljević, S., Đokić, J., Popović, N., Radojević, D., Strahinić, I.,& Golić, N.. (2021). Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria. in Scientific Reports
Nature Portfolio, Berlin., 11(1).
https://doi.org/10.1038/s41598-021-00698-5

Dinić M, Jakovljević S, Đokić J, Popović N, Radojević D, Strahinić I, Golić N. Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria. in Scientific Reports. 2021;11(1).
doi:10.1038/s41598-021-00698-5 .

Dinić, Miroslav, Jakovljević, Stefan, Đokić, Jelena, Popović, Nikola, Radojević, Dušan, Strahinić, Ivana, Golić, Nataša, "Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria" in Scientific Reports, 11, no. 1 (2021),
https://doi.org/10.1038/s41598-021-00698-5 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Lukić, Jovanka
AU  - Lozo, Jelena
AU  - Fira, Đorđe
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Popović, Nikola
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1353
AB  - The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.
PB  - Elsevier, Amsterdam
T2  - Food Research International
T1  - Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties
VL  - 136
DO  - 10.1016/j.foodres.2020.109494
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Tolinački, Maja and Živković, Milica and Lukić, Jovanka and Lozo, Jelena and Fira, Đorđe and Jovčić, Branko and Strahinić, Ivana and Begović, Jelena and Popović, Nikola and Miljković, Marija and Kojić, Milan and Topisirović, Ljubiša and Golić, Nataša",
year = "2020",
abstract = "The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.",
publisher = "Elsevier, Amsterdam",
journal = "Food Research International",
title = "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties",
volume = "136",
doi = "10.1016/j.foodres.2020.109494"
}

Terzić-Vidojević, A., Veljović, K., Tolinački, M., Živković, M., Lukić, J., Lozo, J., Fira, Đ., Jovčić, B., Strahinić, I., Begović, J., Popović, N., Miljković, M., Kojić, M., Topisirović, L.,& Golić, N.. (2020). Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International
Elsevier, Amsterdam., 136.
https://doi.org/10.1016/j.foodres.2020.109494

Terzić-Vidojević A, Veljović K, Tolinački M, Živković M, Lukić J, Lozo J, Fira Đ, Jovčić B, Strahinić I, Begović J, Popović N, Miljković M, Kojić M, Topisirović L, Golić N. Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International. 2020;136.
doi:10.1016/j.foodres.2020.109494 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Tolinački, Maja, Živković, Milica, Lukić, Jovanka, Lozo, Jelena, Fira, Đorđe, Jovčić, Branko, Strahinić, Ivana, Begović, Jelena, Popović, Nikola, Miljković, Marija, Kojić, Milan, Topisirović, Ljubiša, Golić, Nataša, "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties" in Food Research International, 136 (2020),
https://doi.org/10.1016/j.foodres.2020.109494 . .

TY  - JOUR
AU  - Zavisić, Gordana N.
AU  - Petricević, Sasa M.
AU  - Ristić, Slavica M.
AU  - Rikalović, Milena G.
AU  - Jovanović-Ljesković, Nataša M.
AU  - Begović, Jelena
AU  - Strahinić, Ivana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1268
AB  - The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL(-1) units, respectively), moderate adhesion to hexadecane (39 +/- 2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns
EP  - 376
IS  - 4
SP  - 365
VL  - 84
DO  - 10.2298/JSC181105015Z
ER  - 

@article{
author = "Zavisić, Gordana N. and Petricević, Sasa M. and Ristić, Slavica M. and Rikalović, Milena G. and Jovanović-Ljesković, Nataša M. and Begović, Jelena and Strahinić, Ivana",
year = "2019",
abstract = "The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL(-1) units, respectively), moderate adhesion to hexadecane (39 +/- 2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns",
pages = "376-365",
number = "4",
volume = "84",
doi = "10.2298/JSC181105015Z"
}

Zavisić, G. N., Petricević, S. M., Ristić, S. M., Rikalović, M. G., Jovanović-Ljesković, N. M., Begović, J.,& Strahinić, I.. (2019). Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(4), 365-376.
https://doi.org/10.2298/JSC181105015Z

Zavisić GN, Petricević SM, Ristić SM, Rikalović MG, Jovanović-Ljesković NM, Begović J, Strahinić I. Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns. in Journal of the Serbian Chemical Society. 2019;84(4):365-376.
doi:10.2298/JSC181105015Z .

Zavisić, Gordana N., Petricević, Sasa M., Ristić, Slavica M., Rikalović, Milena G., Jovanović-Ljesković, Nataša M., Begović, Jelena, Strahinić, Ivana, "Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns" in Journal of the Serbian Chemical Society, 84, no. 4 (2019):365-376,
https://doi.org/10.2298/JSC181105015Z . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Lukić, Jovanka
AU  - Đokić, Jelena
AU  - Milenković, Marina
AU  - Strahinić, Ivana
AU  - Golić, Nataša
AU  - Begović, Jelena
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1046
AB  - The aim of this study was to investigate the potential of postbiotics originated from Lactobacillus fermentum BGHV110 strain (HV110) to counteract acetaminophen (APAP)-induced hepatotoxicity in HepG2 cells. This strain was selected according to its autophagy inducing potential, based on previous studies reporting protective role of autophagy in APAP caused cellular damage. Cell viability was assessed using MTT and LDH assays, while autophagy was monitored by qPCR analysis of BECN1, Atg5, p62/SQSTM1, and PINK1 mRNA expression and by Western blot analysis of p62/SQSTM1 and lipidated LC3 accumulation. Our results showed that detrimental effect of APAP on cell viability was suppressed in the presence of HV110 which was linked with increased conversion of LC3 protein and p62/SQSTM1 protein degradation. Additionally, higher p62/SQSTM1 and PINK1 mRNA transcription were noticed in cells co-treated with APAP/HV110, simultaneously. In conclusion, this study suggests that HV110 enhances activation of PINK1-dependent autophagy in HepG2 cells and its eventual co-supplementation with APAP could be potentially used for alleviation of hepatotoxic side effects caused by APAP overdose.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity
VL  - 8
DO  - 10.3389/fmicb.2017.00594
ER  - 

@article{
author = "Dinić, Miroslav and Lukić, Jovanka and Đokić, Jelena and Milenković, Marina and Strahinić, Ivana and Golić, Nataša and Begović, Jelena",
year = "2017",
abstract = "The aim of this study was to investigate the potential of postbiotics originated from Lactobacillus fermentum BGHV110 strain (HV110) to counteract acetaminophen (APAP)-induced hepatotoxicity in HepG2 cells. This strain was selected according to its autophagy inducing potential, based on previous studies reporting protective role of autophagy in APAP caused cellular damage. Cell viability was assessed using MTT and LDH assays, while autophagy was monitored by qPCR analysis of BECN1, Atg5, p62/SQSTM1, and PINK1 mRNA expression and by Western blot analysis of p62/SQSTM1 and lipidated LC3 accumulation. Our results showed that detrimental effect of APAP on cell viability was suppressed in the presence of HV110 which was linked with increased conversion of LC3 protein and p62/SQSTM1 protein degradation. Additionally, higher p62/SQSTM1 and PINK1 mRNA transcription were noticed in cells co-treated with APAP/HV110, simultaneously. In conclusion, this study suggests that HV110 enhances activation of PINK1-dependent autophagy in HepG2 cells and its eventual co-supplementation with APAP could be potentially used for alleviation of hepatotoxic side effects caused by APAP overdose.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity",
volume = "8",
doi = "10.3389/fmicb.2017.00594"
}

Dinić, M., Lukić, J., Đokić, J., Milenković, M., Strahinić, I., Golić, N.,& Begović, J.. (2017). Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fmicb.2017.00594

Dinić M, Lukić J, Đokić J, Milenković M, Strahinić I, Golić N, Begović J. Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.00594 .

Dinić, Miroslav, Lukić, Jovanka, Đokić, Jelena, Milenković, Marina, Strahinić, Ivana, Golić, Nataša, Begović, Jelena, "Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.00594 . .

TY  - JOUR
AU  - Golić, Nataša
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Đokić, Jelena
AU  - Strahinić, Ivana
AU  - Mrvaljević, Igor
AU  - Terzić-Vidojević, Amarela
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1091
AB  - Background: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed. Results: The probiotic culture showed strong in vitro antimicrobial effect on C. difficile (human clinical isolate). In addition, individual strains and the probiotic combination exhibited immunomodulatory activity. The probiotic combination significantly increased the proliferation of GALT lymphocytes. At the other hand, none of the bacterial treatments (individual strains and the combination) induced the production of proinflammatory cytokines IL-6 and IL-1 beta by intestinal epithelial cells, Caco-2. Interestingly, Caco-2 cells exposed to the probiotic combination produced significantly elevated amount of TGF beta pointing to potential protecting effect of the probiotic. In addition, the results of field trial on spontaneously infected goats revealed reduction of C. perfringens in goats (below the detection threshold) after the probiotic treatment. Conclusions: The results of this study indicated that the novel probiotic deserves to be further investigated as a promising antimicrobial agent against C. difficile and C. perfringens.
PB  - BMC, London
T2  - BMC Microbiology
T1  - In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens
VL  - 17
DO  - 10.1186/s12866-017-1015-5
ER  - 

@article{
author = "Golić, Nataša and Veljović, Katarina and Popović, Nikola and Đokić, Jelena and Strahinić, Ivana and Mrvaljević, Igor and Terzić-Vidojević, Amarela",
year = "2017",
abstract = "Background: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed. Results: The probiotic culture showed strong in vitro antimicrobial effect on C. difficile (human clinical isolate). In addition, individual strains and the probiotic combination exhibited immunomodulatory activity. The probiotic combination significantly increased the proliferation of GALT lymphocytes. At the other hand, none of the bacterial treatments (individual strains and the combination) induced the production of proinflammatory cytokines IL-6 and IL-1 beta by intestinal epithelial cells, Caco-2. Interestingly, Caco-2 cells exposed to the probiotic combination produced significantly elevated amount of TGF beta pointing to potential protecting effect of the probiotic. In addition, the results of field trial on spontaneously infected goats revealed reduction of C. perfringens in goats (below the detection threshold) after the probiotic treatment. Conclusions: The results of this study indicated that the novel probiotic deserves to be further investigated as a promising antimicrobial agent against C. difficile and C. perfringens.",
publisher = "BMC, London",
journal = "BMC Microbiology",
title = "In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens",
volume = "17",
doi = "10.1186/s12866-017-1015-5"
}

Golić, N., Veljović, K., Popović, N., Đokić, J., Strahinić, I., Mrvaljević, I.,& Terzić-Vidojević, A.. (2017). In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens. in BMC Microbiology
BMC, London., 17.
https://doi.org/10.1186/s12866-017-1015-5

Golić N, Veljović K, Popović N, Đokić J, Strahinić I, Mrvaljević I, Terzić-Vidojević A. In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens. in BMC Microbiology. 2017;17.
doi:10.1186/s12866-017-1015-5 .

Golić, Nataša, Veljović, Katarina, Popović, Nikola, Đokić, Jelena, Strahinić, Ivana, Mrvaljević, Igor, Terzić-Vidojević, Amarela, "In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens" in BMC Microbiology, 17 (2017),
https://doi.org/10.1186/s12866-017-1015-5 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Jancić, I.
AU  - Mirković, N.
AU  - Bufan, B.
AU  - Đokić, Jelena
AU  - Milenković, M.
AU  - Begović, Jelena
AU  - Strahinić, Ivana
AU  - Lozo, Jelena
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1003
AB  - In the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. Accordingly, the aim of this study was to test whether two lactic acid bacteria (LAB) strains, Lactococcus lactis BGBU1-4 and Lactobacillus salivarius BGHO1, could be applied as safe supplements for Listeria infection. Two major research objectives were set: to compare the effects of BGBU1-4 and BGHO1 on early immune response in gut tissue of Wistar rats co-administered with Listeria monocytogenes ATCC19111 and next, to test how this applies to their usage as therapeutics in acute ATCC19111 infection. Intestinal villi (IV), Peyer's patches (PP) and mesenteric lymph nodes (MLN) were used for the analysis. The results showed that BGHO1 increased the mRNA expression of innate immune markers CD14, interleukin (IL)-1 beta and tumour necrosis factor (TNF)-alpha in PP and IV, and, in parallel, caused a decrease of listeriolysin O (LLO) mRNA expression in same tissues. In MLN of BGHO1 treated rats, LLO expression was increased, along with an increase of the expression of OX-62 mRNA and CD69, pointing to the activation of adaptive immunity. On the other hand, in BGBU1-4 treated rats, there was no reduction of LLO mRNA expression and no induction of innate immunity markers in intestinal tissue. Additionally, CD14 and IL-1 beta, as well as LLO, but not OX-62 mRNA and CD69 expression, were elevated in MLN of BGBU1-4 treated rats. However, when applied therapeutically, both, BGBU1-4 and BGHO1, lowered Listeria count in spleens of infected rats. Our results not only reveal the potential of LAB to ameliorate Listeria infections, but suggest different immunological effects of two different LAB strains, both of which could be effective in Listeria elimination.
PB  - Wageningen Academic Publishers, Wageningen
T2  - Beneficial Microbes
T1  - Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes
EP  - 822
IS  - 5
SP  - 809
VL  - 8
DO  - 10.3920/BM2017.0007
ER  - 

@article{
author = "Lukić, Jovanka and Jancić, I. and Mirković, N. and Bufan, B. and Đokić, Jelena and Milenković, M. and Begović, Jelena and Strahinić, Ivana and Lozo, Jelena",
year = "2017",
abstract = "In the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. Accordingly, the aim of this study was to test whether two lactic acid bacteria (LAB) strains, Lactococcus lactis BGBU1-4 and Lactobacillus salivarius BGHO1, could be applied as safe supplements for Listeria infection. Two major research objectives were set: to compare the effects of BGBU1-4 and BGHO1 on early immune response in gut tissue of Wistar rats co-administered with Listeria monocytogenes ATCC19111 and next, to test how this applies to their usage as therapeutics in acute ATCC19111 infection. Intestinal villi (IV), Peyer's patches (PP) and mesenteric lymph nodes (MLN) were used for the analysis. The results showed that BGHO1 increased the mRNA expression of innate immune markers CD14, interleukin (IL)-1 beta and tumour necrosis factor (TNF)-alpha in PP and IV, and, in parallel, caused a decrease of listeriolysin O (LLO) mRNA expression in same tissues. In MLN of BGHO1 treated rats, LLO expression was increased, along with an increase of the expression of OX-62 mRNA and CD69, pointing to the activation of adaptive immunity. On the other hand, in BGBU1-4 treated rats, there was no reduction of LLO mRNA expression and no induction of innate immunity markers in intestinal tissue. Additionally, CD14 and IL-1 beta, as well as LLO, but not OX-62 mRNA and CD69 expression, were elevated in MLN of BGBU1-4 treated rats. However, when applied therapeutically, both, BGBU1-4 and BGHO1, lowered Listeria count in spleens of infected rats. Our results not only reveal the potential of LAB to ameliorate Listeria infections, but suggest different immunological effects of two different LAB strains, both of which could be effective in Listeria elimination.",
publisher = "Wageningen Academic Publishers, Wageningen",
journal = "Beneficial Microbes",
title = "Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes",
pages = "822-809",
number = "5",
volume = "8",
doi = "10.3920/BM2017.0007"
}

Lukić, J., Jancić, I., Mirković, N., Bufan, B., Đokić, J., Milenković, M., Begović, J., Strahinić, I.,& Lozo, J.. (2017). Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes. in Beneficial Microbes
Wageningen Academic Publishers, Wageningen., 8(5), 809-822.
https://doi.org/10.3920/BM2017.0007

Lukić J, Jancić I, Mirković N, Bufan B, Đokić J, Milenković M, Begović J, Strahinić I, Lozo J. Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes. in Beneficial Microbes. 2017;8(5):809-822.
doi:10.3920/BM2017.0007 .

Lukić, Jovanka, Jancić, I., Mirković, N., Bufan, B., Đokić, Jelena, Milenković, M., Begović, Jelena, Strahinić, Ivana, Lozo, Jelena, "Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes" in Beneficial Microbes, 8, no. 5 (2017):809-822,
https://doi.org/10.3920/BM2017.0007 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Chen, Vivien
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Lev-Tov, Hadar
AU  - Davis, Stephen C.
AU  - Tomić-Canić, Marjana
AU  - Pastar, Irena
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1042
AB  - Probiotics are beneficial microorganisms, known to exert numerous positive effects on human health, primarily in the battle against pathogens. Probiotics have been associated with improved healing of intestinal ulcers, and healing of infected cutaneous wounds. This article reviews the latest findings on probiotics related to their pro-healing properties on gut epithelium and skin. Proven mechanisms by which probiotic bacteria exert their beneficial effects include direct killing of pathogens, competitive displacement of pathogenic bacteria, reinforcement of epithelial barrier, induction of fibroblasts, and epithelial cells' migration and function. Beneficial immunomodulatory effects of probiotics relate to modulation and activation of intraepithelial lymphocytes, natural killer cells, and macrophages through induced production of cytokines. Systemic effects of beneficial bacteria and link between gut microbiota, immune system, and cutaneous health through gut-brain-skin axes are discussed as well. In light of growing antibiotic resistance of pathogens, antibiotic use is becoming less effective in treating cutaneous and systemic infections. This review points to a new perspective and therapeutic potential of beneficial probiotic species as a safe alternative approach for treatment of patients affected by wound healing disorders and cutaneous infections.
PB  - Wiley, Hoboken
T2  - Wound Repair and Regeneration
T1  - Probiotics or pro-healers: the role of beneficial bacteria in tissue repair
EP  - 922
IS  - 6
SP  - 912
VL  - 25
DO  - 10.1111/wrr.12607
ER  - 

@article{
author = "Lukić, Jovanka and Chen, Vivien and Strahinić, Ivana and Begović, Jelena and Lev-Tov, Hadar and Davis, Stephen C. and Tomić-Canić, Marjana and Pastar, Irena",
year = "2017",
abstract = "Probiotics are beneficial microorganisms, known to exert numerous positive effects on human health, primarily in the battle against pathogens. Probiotics have been associated with improved healing of intestinal ulcers, and healing of infected cutaneous wounds. This article reviews the latest findings on probiotics related to their pro-healing properties on gut epithelium and skin. Proven mechanisms by which probiotic bacteria exert their beneficial effects include direct killing of pathogens, competitive displacement of pathogenic bacteria, reinforcement of epithelial barrier, induction of fibroblasts, and epithelial cells' migration and function. Beneficial immunomodulatory effects of probiotics relate to modulation and activation of intraepithelial lymphocytes, natural killer cells, and macrophages through induced production of cytokines. Systemic effects of beneficial bacteria and link between gut microbiota, immune system, and cutaneous health through gut-brain-skin axes are discussed as well. In light of growing antibiotic resistance of pathogens, antibiotic use is becoming less effective in treating cutaneous and systemic infections. This review points to a new perspective and therapeutic potential of beneficial probiotic species as a safe alternative approach for treatment of patients affected by wound healing disorders and cutaneous infections.",
publisher = "Wiley, Hoboken",
journal = "Wound Repair and Regeneration",
title = "Probiotics or pro-healers: the role of beneficial bacteria in tissue repair",
pages = "922-912",
number = "6",
volume = "25",
doi = "10.1111/wrr.12607"
}

Lukić, J., Chen, V., Strahinić, I., Begović, J., Lev-Tov, H., Davis, S. C., Tomić-Canić, M.,& Pastar, I.. (2017). Probiotics or pro-healers: the role of beneficial bacteria in tissue repair. in Wound Repair and Regeneration
Wiley, Hoboken., 25(6), 912-922.
https://doi.org/10.1111/wrr.12607

Lukić J, Chen V, Strahinić I, Begović J, Lev-Tov H, Davis SC, Tomić-Canić M, Pastar I. Probiotics or pro-healers: the role of beneficial bacteria in tissue repair. in Wound Repair and Regeneration. 2017;25(6):912-922.
doi:10.1111/wrr.12607 .

Lukić, Jovanka, Chen, Vivien, Strahinić, Ivana, Begović, Jelena, Lev-Tov, Hadar, Davis, Stephen C., Tomić-Canić, Marjana, Pastar, Irena, "Probiotics or pro-healers: the role of beneficial bacteria in tissue repair" in Wound Repair and Regeneration, 25, no. 6 (2017):912-922,
https://doi.org/10.1111/wrr.12607 . .

TY  - JOUR
AU  - Vukotić, Goran
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Lukić, Jovanka
AU  - Kojić, Milan
AU  - Fira, Đorđe
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/992
AB  - Lactocepins or CEPs are large cell wall bound extracellular proteinases of lactic acid bacteria, involved in protein breakdown and utilization. They are responsible for many health-promoting traits of food products fermented with these organisms, but also essential for probiotic effects of certain strains. Different mesophilic strains selected within the species Lactobacillus zeae, Lb. casei, Lb. rhamnosus, and Lb. plantarum were analyzed for their proteolytic activity towards main fractions of milk proteins-caseins and whey proteins. The strains showing excellent proteolytic features were further examined for presence of corresponding proteinase gene(s). It was found that Lb. zeae LMG17315 possessed catalytic domains of three distinct proteinase genes, unique feature in Lb. casei group, which are similar but not identical to previously characterized prtP and prtR genes. Lb. casei neotype strain ATCC393 was also analysed and based on obtained results its reclassification in taxon Lb. zeae is supported. In addition, we report catalytic domain of prtR-type gene in Lb. plantarum LMG9208, which is first such report in this species, and it is first time that this gene is reported outside Lb. casei group.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Microbiology
T1  - Survey on proteolytic activity and diversity of proteinase genes in mesophilic lactobacilli
EP  - 41
IS  - 1
SP  - 33
VL  - 85
DO  - 10.1134/S002626171601015X
ER  - 

@article{
author = "Vukotić, Goran and Strahinić, Ivana and Begović, Jelena and Lukić, Jovanka and Kojić, Milan and Fira, Đorđe",
year = "2016",
abstract = "Lactocepins or CEPs are large cell wall bound extracellular proteinases of lactic acid bacteria, involved in protein breakdown and utilization. They are responsible for many health-promoting traits of food products fermented with these organisms, but also essential for probiotic effects of certain strains. Different mesophilic strains selected within the species Lactobacillus zeae, Lb. casei, Lb. rhamnosus, and Lb. plantarum were analyzed for their proteolytic activity towards main fractions of milk proteins-caseins and whey proteins. The strains showing excellent proteolytic features were further examined for presence of corresponding proteinase gene(s). It was found that Lb. zeae LMG17315 possessed catalytic domains of three distinct proteinase genes, unique feature in Lb. casei group, which are similar but not identical to previously characterized prtP and prtR genes. Lb. casei neotype strain ATCC393 was also analysed and based on obtained results its reclassification in taxon Lb. zeae is supported. In addition, we report catalytic domain of prtR-type gene in Lb. plantarum LMG9208, which is first such report in this species, and it is first time that this gene is reported outside Lb. casei group.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Microbiology",
title = "Survey on proteolytic activity and diversity of proteinase genes in mesophilic lactobacilli",
pages = "41-33",
number = "1",
volume = "85",
doi = "10.1134/S002626171601015X"
}

Vukotić, G., Strahinić, I., Begović, J., Lukić, J., Kojić, M.,& Fira, Đ.. (2016). Survey on proteolytic activity and diversity of proteinase genes in mesophilic lactobacilli. in Microbiology
Maik Nauka/Interperiodica/Springer, New York., 85(1), 33-41.
https://doi.org/10.1134/S002626171601015X

Vukotić G, Strahinić I, Begović J, Lukić J, Kojić M, Fira Đ. Survey on proteolytic activity and diversity of proteinase genes in mesophilic lactobacilli. in Microbiology. 2016;85(1):33-41.
doi:10.1134/S002626171601015X .

Vukotić, Goran, Strahinić, Ivana, Begović, Jelena, Lukić, Jovanka, Kojić, Milan, Fira, Đorđe, "Survey on proteolytic activity and diversity of proteinase genes in mesophilic lactobacilli" in Microbiology, 85, no. 1 (2016):33-41,
https://doi.org/10.1134/S002626171601015X . .

TY  - JOUR
AU  - Vukotić, Goran
AU  - Matić, Ivana
AU  - Begović, Jelena
AU  - Besu, Irina
AU  - Kojić, Milan
AU  - Đokić, Jelena
AU  - Juranić, Zorica
AU  - Strahinić, Ivana
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/928
AB  - The level of humoral immunoreactivity to total cows' milk proteins (TCMP) in sera of patients suffering from recurrent oral ulcerations, gastrointestinal diseases or haematological malignancies was investigated. TCMP were also hydrolysed with two different species of lactobacilli and dramatic changes in the levels of specific IgG and IgE were found with statistically significant decreases in the levels of specific antibodies in sera from all patient groups. The levels below cut-off values of IgG specific for TCMP hydrolysates were detected in sera from all patients, while values of IgE for hydrolysates obtained with Lactobacillus helveticus BGRA43 and Lactobacillus zeae LMG17315were below cut-off in 85% and 97% of patients, respectively. Competitive ELISA confirmed the specificity of antibodies for immunogenic TCMP epitopes, demonstrating that lactobacilli hydrolyse TCMP by degrading immunogenic epitopes, and could therefore be used in processing of milk proteins to obtain products suitable for patients with altered immune response on TCMP.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Dairy Journal
T1  - Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases
EP  - 7
SP  - 1
VL  - 63
DO  - 10.1016/j.idairyj.2016.07.009
ER  - 

@article{
author = "Vukotić, Goran and Matić, Ivana and Begović, Jelena and Besu, Irina and Kojić, Milan and Đokić, Jelena and Juranić, Zorica and Strahinić, Ivana",
year = "2016",
abstract = "The level of humoral immunoreactivity to total cows' milk proteins (TCMP) in sera of patients suffering from recurrent oral ulcerations, gastrointestinal diseases or haematological malignancies was investigated. TCMP were also hydrolysed with two different species of lactobacilli and dramatic changes in the levels of specific IgG and IgE were found with statistically significant decreases in the levels of specific antibodies in sera from all patient groups. The levels below cut-off values of IgG specific for TCMP hydrolysates were detected in sera from all patients, while values of IgE for hydrolysates obtained with Lactobacillus helveticus BGRA43 and Lactobacillus zeae LMG17315were below cut-off in 85% and 97% of patients, respectively. Competitive ELISA confirmed the specificity of antibodies for immunogenic TCMP epitopes, demonstrating that lactobacilli hydrolyse TCMP by degrading immunogenic epitopes, and could therefore be used in processing of milk proteins to obtain products suitable for patients with altered immune response on TCMP.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Dairy Journal",
title = "Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases",
pages = "7-1",
volume = "63",
doi = "10.1016/j.idairyj.2016.07.009"
}

Vukotić, G., Matić, I., Begović, J., Besu, I., Kojić, M., Đokić, J., Juranić, Z.,& Strahinić, I.. (2016). Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases. in International Dairy Journal
Elsevier Sci Ltd, Oxford., 63, 1-7.
https://doi.org/10.1016/j.idairyj.2016.07.009

Vukotić G, Matić I, Begović J, Besu I, Kojić M, Đokić J, Juranić Z, Strahinić I. Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases. in International Dairy Journal. 2016;63:1-7.
doi:10.1016/j.idairyj.2016.07.009 .

Vukotić, Goran, Matić, Ivana, Begović, Jelena, Besu, Irina, Kojić, Milan, Đokić, Jelena, Juranić, Zorica, Strahinić, Ivana, "Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases" in International Dairy Journal, 63 (2016):1-7,
https://doi.org/10.1016/j.idairyj.2016.07.009 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Dinić, Miroslav
AU  - Marcetić, Mirjana
AU  - Milenković, Marina
AU  - Begović, Jelena
AU  - Strahinić, Ivana
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/955
AB  - Aim of this work was to assess the metabolic and physiological changes that occurred in the hind gut of rats after feeding with soybean carbohydrates alone and in combination with Lactobacillus helveticus BGRA43. Wistar rats were gavaged with soybean flour for 28 days. The parameters assessed included fecal volatile organic compounds, and L-lactate, reducing sugars, proteins, ammonia and water levels in the colonic lumen. The presence of lactic acid (LAB), sulfate reducing (SRB) and methanogenic bacteria was assessed by semi-quantitative PCR. Malondialdehyde levels as well as lymphoid tissue size in ileal and colonic mucosa were also evaluated. On the basics of the results obtained, correlation network was created, setting the parameters tested in research in two metabolic groups: saccharolytic and proteolytic fermentation group. The principal finding of the study is a negative correlation between oral administration of BGRA43 and increase of parameters related to carbohydrate fermentation in the gut, and a positive correlation to factors related to proteolytic fermentation. On the contrary, soybean carbohydrates were correlated with increased values of factors related to carbohydrate catabolism. Different effects of BGRA43 and soybean carbohydrates on metabolic processes in colonic lumen indicate the possibility of applying the BGRA43 in alleviating the gastrointestinal symptoms occurring after consuming hardly digestible carbohydrates.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Effects of soybean carbohydrates and lactobacillus helveticus bgra43 on metabolic processes in rat colon
EP  - 921
IS  - 3
SP  - 903
VL  - 48
DO  - 10.2298/GENSR1603903L
ER  - 

@article{
author = "Lukić, Jovanka and Dinić, Miroslav and Marcetić, Mirjana and Milenković, Marina and Begović, Jelena and Strahinić, Ivana",
year = "2016",
abstract = "Aim of this work was to assess the metabolic and physiological changes that occurred in the hind gut of rats after feeding with soybean carbohydrates alone and in combination with Lactobacillus helveticus BGRA43. Wistar rats were gavaged with soybean flour for 28 days. The parameters assessed included fecal volatile organic compounds, and L-lactate, reducing sugars, proteins, ammonia and water levels in the colonic lumen. The presence of lactic acid (LAB), sulfate reducing (SRB) and methanogenic bacteria was assessed by semi-quantitative PCR. Malondialdehyde levels as well as lymphoid tissue size in ileal and colonic mucosa were also evaluated. On the basics of the results obtained, correlation network was created, setting the parameters tested in research in two metabolic groups: saccharolytic and proteolytic fermentation group. The principal finding of the study is a negative correlation between oral administration of BGRA43 and increase of parameters related to carbohydrate fermentation in the gut, and a positive correlation to factors related to proteolytic fermentation. On the contrary, soybean carbohydrates were correlated with increased values of factors related to carbohydrate catabolism. Different effects of BGRA43 and soybean carbohydrates on metabolic processes in colonic lumen indicate the possibility of applying the BGRA43 in alleviating the gastrointestinal symptoms occurring after consuming hardly digestible carbohydrates.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Effects of soybean carbohydrates and lactobacillus helveticus bgra43 on metabolic processes in rat colon",
pages = "921-903",
number = "3",
volume = "48",
doi = "10.2298/GENSR1603903L"
}

Lukić, J., Dinić, M., Marcetić, M., Milenković, M., Begović, J.,& Strahinić, I.. (2016). Effects of soybean carbohydrates and lactobacillus helveticus bgra43 on metabolic processes in rat colon. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 48(3), 903-921.
https://doi.org/10.2298/GENSR1603903L

Lukić J, Dinić M, Marcetić M, Milenković M, Begović J, Strahinić I. Effects of soybean carbohydrates and lactobacillus helveticus bgra43 on metabolic processes in rat colon. in Genetika-Belgrade. 2016;48(3):903-921.
doi:10.2298/GENSR1603903L .

Lukić, Jovanka, Dinić, Miroslav, Marcetić, Mirjana, Milenković, Marina, Begović, Jelena, Strahinić, Ivana, "Effects of soybean carbohydrates and lactobacillus helveticus bgra43 on metabolic processes in rat colon" in Genetika-Belgrade, 48, no. 3 (2016):903-921,
https://doi.org/10.2298/GENSR1603903L . .

TY  - JOUR
AU  - Živković, Milica
AU  - Miljković, Marija
AU  - Ruas-Madiedo, Patricia
AU  - Strahinić, Ivana
AU  - Tolinački, Maja
AU  - Golić, Nataša
AU  - Kojić, Milan
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/886
AB  - Lactobacillus paraplantarum BGCG11, a putative probiotic strain isolated from a soft, white, artisanal cheese, produces a high-molecular-weight heteropolysaccharide, exopolysaccharide (EPS)-CG11, responsible for the ropy phenotype and immunomodulatory activity of the strain. In this study, a 26.4-kb region originating from the pCG1 plasmid, previously shown to be responsible for the production of EPS-CG11 and a ropy phenotype, was cloned, sequenced, and functionally characterized. In this region 16 putative open reading frames (ORFs), encoding enzymes for the production of EPS-CG11, were organized in specific loci involved in the biosynthesis of the repeat unit, polymerization, export, regulation, and chain length determination. Interestingly, downstream of the eps gene cluster, a putative transposase gene was identified, followed by an additional rfb gene cluster containing the rfbACBD genes, the ones most probably responsible for dTDP-L-rhamnose biosynthesis. The functional analysis showed that the production of the high-molecular-weight fraction of EPS-CG11 was absent in two knockout mutants, one in the eps and the other in the rfb gene cluster, as confirmed by size exclusion chromatography analysis. Therefore, both eps and rfb genes clusters are prerequisites for the production of high-molecular-weight EPS-CG11 and for the ropy phenotype of strain L. paraplantarum BGCG11.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Exopolysaccharide Production and Ropy Phenotype Are Determined by Two Gene Clusters in Putative Probiotic Strain Lactobacillus paraplantarum BGCG11
EP  - 1396
IS  - 4
SP  - 1387
VL  - 81
DO  - 10.1128/AEM.03028-14
ER  - 

@article{
author = "Živković, Milica and Miljković, Marija and Ruas-Madiedo, Patricia and Strahinić, Ivana and Tolinački, Maja and Golić, Nataša and Kojić, Milan",
year = "2015",
abstract = "Lactobacillus paraplantarum BGCG11, a putative probiotic strain isolated from a soft, white, artisanal cheese, produces a high-molecular-weight heteropolysaccharide, exopolysaccharide (EPS)-CG11, responsible for the ropy phenotype and immunomodulatory activity of the strain. In this study, a 26.4-kb region originating from the pCG1 plasmid, previously shown to be responsible for the production of EPS-CG11 and a ropy phenotype, was cloned, sequenced, and functionally characterized. In this region 16 putative open reading frames (ORFs), encoding enzymes for the production of EPS-CG11, were organized in specific loci involved in the biosynthesis of the repeat unit, polymerization, export, regulation, and chain length determination. Interestingly, downstream of the eps gene cluster, a putative transposase gene was identified, followed by an additional rfb gene cluster containing the rfbACBD genes, the ones most probably responsible for dTDP-L-rhamnose biosynthesis. The functional analysis showed that the production of the high-molecular-weight fraction of EPS-CG11 was absent in two knockout mutants, one in the eps and the other in the rfb gene cluster, as confirmed by size exclusion chromatography analysis. Therefore, both eps and rfb genes clusters are prerequisites for the production of high-molecular-weight EPS-CG11 and for the ropy phenotype of strain L. paraplantarum BGCG11.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Exopolysaccharide Production and Ropy Phenotype Are Determined by Two Gene Clusters in Putative Probiotic Strain Lactobacillus paraplantarum BGCG11",
pages = "1396-1387",
number = "4",
volume = "81",
doi = "10.1128/AEM.03028-14"
}

Živković, M., Miljković, M., Ruas-Madiedo, P., Strahinić, I., Tolinački, M., Golić, N.,& Kojić, M.. (2015). Exopolysaccharide Production and Ropy Phenotype Are Determined by Two Gene Clusters in Putative Probiotic Strain Lactobacillus paraplantarum BGCG11. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 81(4), 1387-1396.
https://doi.org/10.1128/AEM.03028-14

Živković M, Miljković M, Ruas-Madiedo P, Strahinić I, Tolinački M, Golić N, Kojić M. Exopolysaccharide Production and Ropy Phenotype Are Determined by Two Gene Clusters in Putative Probiotic Strain Lactobacillus paraplantarum BGCG11. in Applied and Environmental Microbiology. 2015;81(4):1387-1396.
doi:10.1128/AEM.03028-14 .

Živković, Milica, Miljković, Marija, Ruas-Madiedo, Patricia, Strahinić, Ivana, Tolinački, Maja, Golić, Nataša, Kojić, Milan, "Exopolysaccharide Production and Ropy Phenotype Are Determined by Two Gene Clusters in Putative Probiotic Strain Lactobacillus paraplantarum BGCG11" in Applied and Environmental Microbiology, 81, no. 4 (2015):1387-1396,
https://doi.org/10.1128/AEM.03028-14 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran 
AU  - Pastor, Ferenc T.
AU  - Suznjević, Desanka
AU  - Jovanović, Živko
AU  - Strahinić, Ivana
AU  - Fira, Đorđe
AU  - Radović, Svetlana S.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/845
AB  - Nine Lactobacillus strains known for surface proteinase activity were chosen from our collection and tested for their ability to grow in pea seed protein-based medium, and to hydrolyze purified pea proteins in order to produce peptides with antioxidant (AO) activity. Two strains, Lactobacillus rhamnosus BGT10 and Lactobacillus zeae LMG17315, exhibited strong proteolytic activity against pea proteins. The AO activity of the pea hydrolysate fraction, MW  lt  10 kDa, obtained by the fermentation of purified pea proteins with Lactobacillus rhamnosus BGT10, was tested by standard spectrophotometric assays (DPPH, ABTS, Fe3+-reducing capacity) and the recently developed direct current (DC) polarographic assay. The low molecular weight fraction of the obtained hydrolysate was separated using ion exchange chromatography, while the AO activity of eluted fractions was determined by means of a sensitive DC polarographic assay without previous concentration of samples. Results revealed that the fraction present in low abundance that contained basic peptides possessed the highest antioxidant activity. Based on the obtained results, it can be concluded that Lactobacillus rhamnosus BGT10 should be further investigated as a candidate strain for large-scale production of bioactive peptides from legume proteins.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria
EP  - 1042
IS  - 3
SP  - 1033
VL  - 67
DO  - 10.2298/ABS150130066S
ER  - 

@article{
author = "Stanisavljević, Nemanja and Vukotić, Goran  and Pastor, Ferenc T. and Suznjević, Desanka and Jovanović, Živko and Strahinić, Ivana and Fira, Đorđe and Radović, Svetlana S.",
year = "2015",
abstract = "Nine Lactobacillus strains known for surface proteinase activity were chosen from our collection and tested for their ability to grow in pea seed protein-based medium, and to hydrolyze purified pea proteins in order to produce peptides with antioxidant (AO) activity. Two strains, Lactobacillus rhamnosus BGT10 and Lactobacillus zeae LMG17315, exhibited strong proteolytic activity against pea proteins. The AO activity of the pea hydrolysate fraction, MW  lt  10 kDa, obtained by the fermentation of purified pea proteins with Lactobacillus rhamnosus BGT10, was tested by standard spectrophotometric assays (DPPH, ABTS, Fe3+-reducing capacity) and the recently developed direct current (DC) polarographic assay. The low molecular weight fraction of the obtained hydrolysate was separated using ion exchange chromatography, while the AO activity of eluted fractions was determined by means of a sensitive DC polarographic assay without previous concentration of samples. Results revealed that the fraction present in low abundance that contained basic peptides possessed the highest antioxidant activity. Based on the obtained results, it can be concluded that Lactobacillus rhamnosus BGT10 should be further investigated as a candidate strain for large-scale production of bioactive peptides from legume proteins.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria",
pages = "1042-1033",
number = "3",
volume = "67",
doi = "10.2298/ABS150130066S"
}

Stanisavljević, N., Vukotić, G., Pastor, F. T., Suznjević, D., Jovanović, Ž., Strahinić, I., Fira, Đ.,& Radović, S. S.. (2015). Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(3), 1033-1042.
https://doi.org/10.2298/ABS150130066S

Stanisavljević N, Vukotić G, Pastor FT, Suznjević D, Jovanović Ž, Strahinić I, Fira Đ, Radović SS. Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria. in Archives of Biological Sciences. 2015;67(3):1033-1042.
doi:10.2298/ABS150130066S .

Stanisavljević, Nemanja, Vukotić, Goran , Pastor, Ferenc T., Suznjević, Desanka, Jovanović, Živko, Strahinić, Ivana, Fira, Đorđe, Radović, Svetlana S., "Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria" in Archives of Biological Sciences, 67, no. 3 (2015):1033-1042,
https://doi.org/10.2298/ABS150130066S . .

TY  - JOUR
AU  - Vukotić, Goran
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
AU  - Miljković, Marija
AU  - Strahinić, Ivana
AU  - Fira, Đorđe
AU  - Radulović, Zorica
AU  - Kojić, Milan
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/895
AB  - Proteinases and bacteriocins are of great importance to the dairy industry, but their interactions have not been studied so far. Lactococcus lactis subsp. lactis BGMN1-5 is a natural isolate from homemade semi-hard cheese which produces two bacteriocins (Lactococcin B and LsbB), as well as proteinase PrtP. A medium-dependent increase in the bacteriocin LcnB activity of L. lactis BGMN1-501, a derivate of L. lactis subsp. lactis BGMN1-5, was shown to be accompanied by a decrease in its promoter activity. A similar effect of media components on gene expression was reported for proteinase PrtP whose gene is co-localized on the same plasmid as the IcnB gene. Thus, the PrtP-LcnB interplay was investigated. Single gene knockout mutants were constructed with disrupted prtP or IcnB genes. PrtP mutants showed higher bacteriocin activity that had lost its growth medium dependence, which was in contrast to the original strain. When LcnB from this mutant was combined with proteinase from the LonB(-) mutant in vitro, its activity was rendered to the original level, suggesting that proteinase reduces bacteriocin activity. We propose a new model of medium dependent expression of these genes with regard to the effects of their interaction in vivo.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Proteinase PrtP impairs lactococcin LcnB activity in Lactococcus lactis BGMN1-501: new insights into bacteriocin regulation
VL  - 6
DO  - 10.3389/fmicb.2015.00092
ER  - 

@article{
author = "Vukotić, Goran and Mirković, Nemanja and Jovčić, Branko and Miljković, Marija and Strahinić, Ivana and Fira, Đorđe and Radulović, Zorica and Kojić, Milan",
year = "2015",
abstract = "Proteinases and bacteriocins are of great importance to the dairy industry, but their interactions have not been studied so far. Lactococcus lactis subsp. lactis BGMN1-5 is a natural isolate from homemade semi-hard cheese which produces two bacteriocins (Lactococcin B and LsbB), as well as proteinase PrtP. A medium-dependent increase in the bacteriocin LcnB activity of L. lactis BGMN1-501, a derivate of L. lactis subsp. lactis BGMN1-5, was shown to be accompanied by a decrease in its promoter activity. A similar effect of media components on gene expression was reported for proteinase PrtP whose gene is co-localized on the same plasmid as the IcnB gene. Thus, the PrtP-LcnB interplay was investigated. Single gene knockout mutants were constructed with disrupted prtP or IcnB genes. PrtP mutants showed higher bacteriocin activity that had lost its growth medium dependence, which was in contrast to the original strain. When LcnB from this mutant was combined with proteinase from the LonB(-) mutant in vitro, its activity was rendered to the original level, suggesting that proteinase reduces bacteriocin activity. We propose a new model of medium dependent expression of these genes with regard to the effects of their interaction in vivo.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Proteinase PrtP impairs lactococcin LcnB activity in Lactococcus lactis BGMN1-501: new insights into bacteriocin regulation",
volume = "6",
doi = "10.3389/fmicb.2015.00092"
}

Vukotić, G., Mirković, N., Jovčić, B., Miljković, M., Strahinić, I., Fira, Đ., Radulović, Z.,& Kojić, M.. (2015). Proteinase PrtP impairs lactococcin LcnB activity in Lactococcus lactis BGMN1-501: new insights into bacteriocin regulation. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 6.
https://doi.org/10.3389/fmicb.2015.00092

Vukotić G, Mirković N, Jovčić B, Miljković M, Strahinić I, Fira Đ, Radulović Z, Kojić M. Proteinase PrtP impairs lactococcin LcnB activity in Lactococcus lactis BGMN1-501: new insights into bacteriocin regulation. in Frontiers in Microbiology. 2015;6.
doi:10.3389/fmicb.2015.00092 .

Vukotić, Goran, Mirković, Nemanja, Jovčić, Branko, Miljković, Marija, Strahinić, Ivana, Fira, Đorđe, Radulović, Zorica, Kojić, Milan, "Proteinase PrtP impairs lactococcin LcnB activity in Lactococcus lactis BGMN1-501: new insights into bacteriocin regulation" in Frontiers in Microbiology, 6 (2015),
https://doi.org/10.3389/fmicb.2015.00092 . .

TY  - JOUR
AU  - Miljković, Marija
AU  - Strahinić, Ivana
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Kojić, Snežana
AU  - Golić, Nataša
AU  - Kojić, Milan
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/805
AB  - Eleven Lactobacillus strains with strong aggregation abilities were selected from a laboratory collection. In two of the strains, genes associated with aggregation capability were plasmid located and found to strongly correlate with collagen binding. The gene encoding the auto-aggregation-promoting protein (AggLb) of Lactobacillus paracasei subsp. paracasei BGNJ1-64 was cloned using a novel, wide-range-host shuttle cloning vector, pAZILSJ. The clone pALb35, containing a 11377-bp DNA fragment, was selected from the SacI plasmid library for its ability to provide carriers with the aggregation phenotype. The complete fragment was sequenced and four potential ORFs were detected, including the aggLb gene and three surrounding transposase genes. AggLb is the largest known cell-surface protein in lactobacilli, consisting of 2998 aa (318,611 Da). AggLb belongs to the collagen-binding superfamily and its C-terminal region contains 20 successive repeats that are identical even at the nucleotide level. Deletion of aggLb causes a loss of the capacity to form cell aggregates, whereas overexpression increases cellular aggregation, hydrophobicity and collagen-binding potential. PCR screening performed with three sets of primers based on the aggLb gene of BGNJ1-64 enabled detection of the same type of aggLb gene in five of eleven selected aggregation-positive Lactobacillus strains. Heterologous expression of aggLb confirmed the crucial role of the AggLb protein in cell aggregation and specific collagen binding, indicating that AggLb has a useful probiotic function in effective colonization of host tissue and prevention of pathogen colonization.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - AggLb Is the Largest Cell-Aggregation Factor from Lactobacillus paracasei Subsp paracasei BGNJ1-64, Functions in Collagen Adhesion, and Pathogen Exclusion In Vitro
IS  - 5
VL  - 10
DO  - 10.1371/journal.pone.0126387
ER  - 

@article{
author = "Miljković, Marija and Strahinić, Ivana and Tolinački, Maja and Živković, Milica and Kojić, Snežana and Golić, Nataša and Kojić, Milan",
year = "2015",
abstract = "Eleven Lactobacillus strains with strong aggregation abilities were selected from a laboratory collection. In two of the strains, genes associated with aggregation capability were plasmid located and found to strongly correlate with collagen binding. The gene encoding the auto-aggregation-promoting protein (AggLb) of Lactobacillus paracasei subsp. paracasei BGNJ1-64 was cloned using a novel, wide-range-host shuttle cloning vector, pAZILSJ. The clone pALb35, containing a 11377-bp DNA fragment, was selected from the SacI plasmid library for its ability to provide carriers with the aggregation phenotype. The complete fragment was sequenced and four potential ORFs were detected, including the aggLb gene and three surrounding transposase genes. AggLb is the largest known cell-surface protein in lactobacilli, consisting of 2998 aa (318,611 Da). AggLb belongs to the collagen-binding superfamily and its C-terminal region contains 20 successive repeats that are identical even at the nucleotide level. Deletion of aggLb causes a loss of the capacity to form cell aggregates, whereas overexpression increases cellular aggregation, hydrophobicity and collagen-binding potential. PCR screening performed with three sets of primers based on the aggLb gene of BGNJ1-64 enabled detection of the same type of aggLb gene in five of eleven selected aggregation-positive Lactobacillus strains. Heterologous expression of aggLb confirmed the crucial role of the AggLb protein in cell aggregation and specific collagen binding, indicating that AggLb has a useful probiotic function in effective colonization of host tissue and prevention of pathogen colonization.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "AggLb Is the Largest Cell-Aggregation Factor from Lactobacillus paracasei Subsp paracasei BGNJ1-64, Functions in Collagen Adhesion, and Pathogen Exclusion In Vitro",
number = "5",
volume = "10",
doi = "10.1371/journal.pone.0126387"
}

Miljković, M., Strahinić, I., Tolinački, M., Živković, M., Kojić, S., Golić, N.,& Kojić, M.. (2015). AggLb Is the Largest Cell-Aggregation Factor from Lactobacillus paracasei Subsp paracasei BGNJ1-64, Functions in Collagen Adhesion, and Pathogen Exclusion In Vitro. in PLoS One
Public Library Science, San Francisco., 10(5).
https://doi.org/10.1371/journal.pone.0126387

Miljković M, Strahinić I, Tolinački M, Živković M, Kojić S, Golić N, Kojić M. AggLb Is the Largest Cell-Aggregation Factor from Lactobacillus paracasei Subsp paracasei BGNJ1-64, Functions in Collagen Adhesion, and Pathogen Exclusion In Vitro. in PLoS One. 2015;10(5).
doi:10.1371/journal.pone.0126387 .

Miljković, Marija, Strahinić, Ivana, Tolinački, Maja, Živković, Milica, Kojić, Snežana, Golić, Nataša, Kojić, Milan, "AggLb Is the Largest Cell-Aggregation Factor from Lactobacillus paracasei Subsp paracasei BGNJ1-64, Functions in Collagen Adhesion, and Pathogen Exclusion In Vitro" in PLoS One, 10, no. 5 (2015),
https://doi.org/10.1371/journal.pone.0126387 . .

TY  - CONF
AU  - Zavisić, G.
AU  - Ristić, S.
AU  - Petricević, S.
AU  - Jovanović, J. Novakovic
AU  - Radulović, Z.
AU  - Petković, B. Janac
AU  - Strahinić, Ivana
AU  - Piperski, V.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/888
AB  - We investigated the potential probiotic properties of indigenous lactic acid bacteria (LAB) isolated from Serbian homemade cheese. Seventeen LAB strains were isolated and characterised using standard protocols. One of the strains showed several probiotic properties: survival at low pH and in bile salts solution, antimicrobial activity, susceptibility to antibiotics and adhesion to hexodecane. DNA analysis identified the isolate as Lactobacillus casei, hereafter named Lactobacillus casei 5s. The lipid lowering effect of Lactobacillus casei 5s was evaluated in vivo using a hyperlipidemic rat model. Orally administered Lactobacillus casei 5s significantly decreased the elevated total serum cholesterol and triglycerides, and attenuated macro vesicular steatosis in the liver. Moreover, Lactobacillus casei 5s improved the intestinal microbial balance in favour of lactobacilli, while decreasing the number of Escherichia coli cells. The bacteria were re-isolated and identified from the surface of the intestinal mucosa and from the faecal samples of treated animals, indicating adhesiveness and colonisation ability. The results of an acute oral toxicity study in mice and the absence of translocation to other organs demonstrated the safety of the strain. In conclusion, Lactobacillus casei 5s demonstrated promising probiotic potential and might be a good candidate for more detailed investigations.
PB  - Wageningen Academic Publishers, Wageningen
C3  - Beneficial Microbes
T1  - Characterisation and preliminary lipid-lowering evaluation of Lactobacillus isolated from a traditional Serbian dairy product
EP  - 128
IS  - 1
SP  - 119
VL  - 6
DO  - 10.3920/BM2014.0018
ER  - 

@conference{
author = "Zavisić, G. and Ristić, S. and Petricević, S. and Jovanović, J. Novakovic and Radulović, Z. and Petković, B. Janac and Strahinić, Ivana and Piperski, V.",
year = "2015",
abstract = "We investigated the potential probiotic properties of indigenous lactic acid bacteria (LAB) isolated from Serbian homemade cheese. Seventeen LAB strains were isolated and characterised using standard protocols. One of the strains showed several probiotic properties: survival at low pH and in bile salts solution, antimicrobial activity, susceptibility to antibiotics and adhesion to hexodecane. DNA analysis identified the isolate as Lactobacillus casei, hereafter named Lactobacillus casei 5s. The lipid lowering effect of Lactobacillus casei 5s was evaluated in vivo using a hyperlipidemic rat model. Orally administered Lactobacillus casei 5s significantly decreased the elevated total serum cholesterol and triglycerides, and attenuated macro vesicular steatosis in the liver. Moreover, Lactobacillus casei 5s improved the intestinal microbial balance in favour of lactobacilli, while decreasing the number of Escherichia coli cells. The bacteria were re-isolated and identified from the surface of the intestinal mucosa and from the faecal samples of treated animals, indicating adhesiveness and colonisation ability. The results of an acute oral toxicity study in mice and the absence of translocation to other organs demonstrated the safety of the strain. In conclusion, Lactobacillus casei 5s demonstrated promising probiotic potential and might be a good candidate for more detailed investigations.",
publisher = "Wageningen Academic Publishers, Wageningen",
journal = "Beneficial Microbes",
title = "Characterisation and preliminary lipid-lowering evaluation of Lactobacillus isolated from a traditional Serbian dairy product",
pages = "128-119",
number = "1",
volume = "6",
doi = "10.3920/BM2014.0018"
}

Zavisić, G., Ristić, S., Petricević, S., Jovanović, J. N., Radulović, Z., Petković, B. J., Strahinić, I.,& Piperski, V.. (2015). Characterisation and preliminary lipid-lowering evaluation of Lactobacillus isolated from a traditional Serbian dairy product. in Beneficial Microbes
Wageningen Academic Publishers, Wageningen., 6(1), 119-128.
https://doi.org/10.3920/BM2014.0018

Zavisić G, Ristić S, Petricević S, Jovanović JN, Radulović Z, Petković BJ, Strahinić I, Piperski V. Characterisation and preliminary lipid-lowering evaluation of Lactobacillus isolated from a traditional Serbian dairy product. in Beneficial Microbes. 2015;6(1):119-128.
doi:10.3920/BM2014.0018 .

Zavisić, G., Ristić, S., Petricević, S., Jovanović, J. Novakovic, Radulović, Z., Petković, B. Janac, Strahinić, Ivana, Piperski, V., "Characterisation and preliminary lipid-lowering evaluation of Lactobacillus isolated from a traditional Serbian dairy product" in Beneficial Microbes, 6, no. 1 (2015):119-128,
https://doi.org/10.3920/BM2014.0018 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Tonković, Katarina
AU  - Pavunc, Andreja Lebos
AU  - Beganović, Jasna
AU  - Strahinić, Ivana
AU  - Kojić, Milan
AU  - Veljović, Katarina
AU  - Golić, Nataša
AU  - Kos, Blazenka
AU  - Cadez, Neza
AU  - Gregurek, Ljerka
AU  - Susković, Jagoda
AU  - Raspor, Peter
AU  - Topisirović, Ljubiša
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/799
AB  - In order to preserve the traditional manufacturing of white pickled (WPC) and fresh soft cheeses (FSC), well-characterized autochthonous lactic acid bacteria (LAB) with advantageous characteristics were applied for the production of the cheeses at small industrial scale under the controlled conditions. Selected LAB for design of defined mixed starter cultures belonged to Lactococcus lactis ZGBP5-9, Enterococcus faecium ZGPR1-54 and Lactobacillus plantarum ZGPR2-25 for FSC production and to Lc. lactis BGAL1-4, Lactobacillus brevis BGGO7-28 and Lb. plantarum BGGO7-29 for WPC production. A sensory evaluation indicated that the cheeses obtained by inoculation with selected autochthonous LAB are similar to the traditional cheese and received the best scores. Viable cell counts of LAB used for the production of both type chesses was high, over 10(6) cfu g(-1). High viability of the surveyed strains was supported with PCR-DGGE, which confirm the retention of selected LAB strains as starter cultures in cheese production. Next, PFGE analysis showed that each single strains, selected in particular cheese mixed culture, revealed unique SmaI PFGE pattern that could enable efficient discrimination and monitoring of the strains in industrial process. As some of the selected LAB strains are attributed as potential probiotics, produced cheeses could be considered as functional food.
PB  - Elsevier, Amsterdam
T2  - Lwt-Food Science and Technology
T1  - Evaluation of autochthonous lactic acid bacteria as starter cultures for production of white pickled and fresh soft cheeses
EP  - 306
IS  - 1
SP  - 298
VL  - 63
DO  - 10.1016/j.lwt.2015.03.050
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Tonković, Katarina and Pavunc, Andreja Lebos and Beganović, Jasna and Strahinić, Ivana and Kojić, Milan and Veljović, Katarina and Golić, Nataša and Kos, Blazenka and Cadez, Neza and Gregurek, Ljerka and Susković, Jagoda and Raspor, Peter and Topisirović, Ljubiša",
year = "2015",
abstract = "In order to preserve the traditional manufacturing of white pickled (WPC) and fresh soft cheeses (FSC), well-characterized autochthonous lactic acid bacteria (LAB) with advantageous characteristics were applied for the production of the cheeses at small industrial scale under the controlled conditions. Selected LAB for design of defined mixed starter cultures belonged to Lactococcus lactis ZGBP5-9, Enterococcus faecium ZGPR1-54 and Lactobacillus plantarum ZGPR2-25 for FSC production and to Lc. lactis BGAL1-4, Lactobacillus brevis BGGO7-28 and Lb. plantarum BGGO7-29 for WPC production. A sensory evaluation indicated that the cheeses obtained by inoculation with selected autochthonous LAB are similar to the traditional cheese and received the best scores. Viable cell counts of LAB used for the production of both type chesses was high, over 10(6) cfu g(-1). High viability of the surveyed strains was supported with PCR-DGGE, which confirm the retention of selected LAB strains as starter cultures in cheese production. Next, PFGE analysis showed that each single strains, selected in particular cheese mixed culture, revealed unique SmaI PFGE pattern that could enable efficient discrimination and monitoring of the strains in industrial process. As some of the selected LAB strains are attributed as potential probiotics, produced cheeses could be considered as functional food.",
publisher = "Elsevier, Amsterdam",
journal = "Lwt-Food Science and Technology",
title = "Evaluation of autochthonous lactic acid bacteria as starter cultures for production of white pickled and fresh soft cheeses",
pages = "306-298",
number = "1",
volume = "63",
doi = "10.1016/j.lwt.2015.03.050"
}

Terzić-Vidojević, A., Tonković, K., Pavunc, A. L., Beganović, J., Strahinić, I., Kojić, M., Veljović, K., Golić, N., Kos, B., Cadez, N., Gregurek, L., Susković, J., Raspor, P.,& Topisirović, L.. (2015). Evaluation of autochthonous lactic acid bacteria as starter cultures for production of white pickled and fresh soft cheeses. in Lwt-Food Science and Technology
Elsevier, Amsterdam., 63(1), 298-306.
https://doi.org/10.1016/j.lwt.2015.03.050

Terzić-Vidojević A, Tonković K, Pavunc AL, Beganović J, Strahinić I, Kojić M, Veljović K, Golić N, Kos B, Cadez N, Gregurek L, Susković J, Raspor P, Topisirović L. Evaluation of autochthonous lactic acid bacteria as starter cultures for production of white pickled and fresh soft cheeses. in Lwt-Food Science and Technology. 2015;63(1):298-306.
doi:10.1016/j.lwt.2015.03.050 .

Terzić-Vidojević, Amarela, Tonković, Katarina, Pavunc, Andreja Lebos, Beganović, Jasna, Strahinić, Ivana, Kojić, Milan, Veljović, Katarina, Golić, Nataša, Kos, Blazenka, Cadez, Neza, Gregurek, Ljerka, Susković, Jagoda, Raspor, Peter, Topisirović, Ljubiša, "Evaluation of autochthonous lactic acid bacteria as starter cultures for production of white pickled and fresh soft cheeses" in Lwt-Food Science and Technology, 63, no. 1 (2015):298-306,
https://doi.org/10.1016/j.lwt.2015.03.050 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Strahinić, Ivana
AU  - Milenković, Marina
AU  - Živković, Milica
AU  - Tolinački, Maja
AU  - Kojić, Milan
AU  - Begović, Jelena
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/748
AB  - Modern research in the area of probiotics is largely devoted to discovering factors that promote the adherence of probiotic candidates to host mucosal surfaces. The aim of the present study was to test the role of aggregation factor (AggL) and mucin-binding protein (MbpL) from Lactococcus sp. in adhesion to gastrointestinal mucosa. In vitro, ex vivo, and in vivo experiments in rats were used to assess the adhesive potential of these two proteins expressed in heterologous host Lactobacillus salivarius BGHO1. Although there was no influence of MbpL protein expression on BGHO1 adhesion to gut mucosa, expression of AggL had a negative effect on BGHO1 binding to ileal and colonic rat mucosa, as well as to human HT29-MTX cells and porcine gastric mucin in vitro. Because AggL did not decrease the adhesion of bacteria to intestinal fragments in ex vivo tests, where peristaltic simulation conditions were missing, we propose that intestinal motility could be a crucial force for eliminating aggregation-factor-bearing bacteria. Bacterial strains expressing aggregation factor could facilitate the removal of pathogens through the coaggregation mechanism, thus balancing gut microbial ecosystems in people affected by intestinal bacteria overgrowth.
PB  - Springer, New York
T2  - Microbial Ecology
T1  - Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa
EP  - 644
IS  - 3
SP  - 633
VL  - 68
DO  - 10.1007/s00248-014-0426-1
ER  - 

@article{
author = "Lukić, Jovanka and Strahinić, Ivana and Milenković, Marina and Živković, Milica and Tolinački, Maja and Kojić, Milan and Begović, Jelena",
year = "2014",
abstract = "Modern research in the area of probiotics is largely devoted to discovering factors that promote the adherence of probiotic candidates to host mucosal surfaces. The aim of the present study was to test the role of aggregation factor (AggL) and mucin-binding protein (MbpL) from Lactococcus sp. in adhesion to gastrointestinal mucosa. In vitro, ex vivo, and in vivo experiments in rats were used to assess the adhesive potential of these two proteins expressed in heterologous host Lactobacillus salivarius BGHO1. Although there was no influence of MbpL protein expression on BGHO1 adhesion to gut mucosa, expression of AggL had a negative effect on BGHO1 binding to ileal and colonic rat mucosa, as well as to human HT29-MTX cells and porcine gastric mucin in vitro. Because AggL did not decrease the adhesion of bacteria to intestinal fragments in ex vivo tests, where peristaltic simulation conditions were missing, we propose that intestinal motility could be a crucial force for eliminating aggregation-factor-bearing bacteria. Bacterial strains expressing aggregation factor could facilitate the removal of pathogens through the coaggregation mechanism, thus balancing gut microbial ecosystems in people affected by intestinal bacteria overgrowth.",
publisher = "Springer, New York",
journal = "Microbial Ecology",
title = "Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa",
pages = "644-633",
number = "3",
volume = "68",
doi = "10.1007/s00248-014-0426-1"
}

Lukić, J., Strahinić, I., Milenković, M., Živković, M., Tolinački, M., Kojić, M.,& Begović, J.. (2014). Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa. in Microbial Ecology
Springer, New York., 68(3), 633-644.
https://doi.org/10.1007/s00248-014-0426-1

Lukić J, Strahinić I, Milenković M, Živković M, Tolinački M, Kojić M, Begović J. Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa. in Microbial Ecology. 2014;68(3):633-644.
doi:10.1007/s00248-014-0426-1 .

Lukić, Jovanka, Strahinić, Ivana, Milenković, Marina, Živković, Milica, Tolinački, Maja, Kojić, Milan, Begović, Jelena, "Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa" in Microbial Ecology, 68, no. 3 (2014):633-644,
https://doi.org/10.1007/s00248-014-0426-1 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Strahinić, Ivana
AU  - Milenković, Marina
AU  - Golić, Nataša
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
AU  - Begović, Jelena
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/657
AB  - The present study was carried out to test the colonic mucosal response of rats to oral supplementation with Lactobacillus fermentum BGHI14 and to correlate the tissue reaction to trinitrobenzenesulfonate (TNBS)-induced colitis with mucosal barrier alterations caused by bacterial ingestion. An immune cell-mediated reaction of healthy colonic tissue was noticed after bacterial feeding. After prolonged bacterial treatment, the observed reaction had retreated to normality, but the mRNA levels of proinflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) remained elevated. These data point to the chronic low-grade inflammation that could be caused by long-term probiotic consumption. Although no detrimental effects of bacterial pretreatment were noticed in colitic rats, at least in the acute state of disease, the results obtained in our study point to the necessity of reassessment of existing data on the safety of probiotic preparations. Additionally, probiotic effects in experimental colitis models might depend on time coordination of disease induction with treatment duration.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction
EP  - 5744
IS  - 18
SP  - 5735
VL  - 79
DO  - 10.1128/AEM.01807-13
ER  - 

@article{
author = "Lukić, Jovanka and Strahinić, Ivana and Milenković, Marina and Golić, Nataša and Kojić, Milan and Topisirović, Ljubiša and Begović, Jelena",
year = "2013",
abstract = "The present study was carried out to test the colonic mucosal response of rats to oral supplementation with Lactobacillus fermentum BGHI14 and to correlate the tissue reaction to trinitrobenzenesulfonate (TNBS)-induced colitis with mucosal barrier alterations caused by bacterial ingestion. An immune cell-mediated reaction of healthy colonic tissue was noticed after bacterial feeding. After prolonged bacterial treatment, the observed reaction had retreated to normality, but the mRNA levels of proinflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) remained elevated. These data point to the chronic low-grade inflammation that could be caused by long-term probiotic consumption. Although no detrimental effects of bacterial pretreatment were noticed in colitic rats, at least in the acute state of disease, the results obtained in our study point to the necessity of reassessment of existing data on the safety of probiotic preparations. Additionally, probiotic effects in experimental colitis models might depend on time coordination of disease induction with treatment duration.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction",
pages = "5744-5735",
number = "18",
volume = "79",
doi = "10.1128/AEM.01807-13"
}

Lukić, J., Strahinić, I., Milenković, M., Golić, N., Kojić, M., Topisirović, L.,& Begović, J.. (2013). Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 79(18), 5735-5744.
https://doi.org/10.1128/AEM.01807-13

Lukić J, Strahinić I, Milenković M, Golić N, Kojić M, Topisirović L, Begović J. Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction. in Applied and Environmental Microbiology. 2013;79(18):5735-5744.
doi:10.1128/AEM.01807-13 .

Lukić, Jovanka, Strahinić, Ivana, Milenković, Marina, Golić, Nataša, Kojić, Milan, Topisirović, Ljubiša, Begović, Jelena, "Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction" in Applied and Environmental Microbiology, 79, no. 18 (2013):5735-5744,
https://doi.org/10.1128/AEM.01807-13 . .

TY  - THES
AU  - Strahinić, Ivana
PY  - 2013
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6883
UR  - https://nardus.mpn.gov.rs/handle/123456789/11423
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:20115/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=25691919
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/29
AB  - Prirodni izolat BGRA43, izolovan iz gastrointestinalnog trakta čoveka, determinisan je na osnovu klasičnih mikrobioloških i molekularno-bioloških metoda kao vrsta Lactobacillus helveticus. Soj poseduje jedan plazmid (pRA1), veličine 2.4 kb, koji se stabilno održava u ćeliji i nakon 10 sukcesivnih presejavanja. Za potrebe genetičkih manipulacija soj je uspešno transformisan plazmidom pA1-6, mada je efikasnost transformacije niska. Optimalna temperatura za rast ovog soja je 42oC, pri čemu je pokazano da soj dobro raste kako u MRS-u tako i u 10% obranom mleku. Nakon 6 sati rasta  u 10% obranom mleku na temperaturi od 37oC dolazi do obaranja pH vrednosti u mleku na 4.53 pri čemu takodje dolazi do formiranja homogenog gruša visokog stepena viskoznosti. Soj BGRA43 pokazuje antimikrobijalnu aktivnost na veći broj Gram-pozitivnih i Gram-negativnih bakterija. Takodje je utvrdjeno da soj BGRA43 poseduje ekstracelularnu proteinazu. Cele ćelije ovog soja hidrolizuju sve tri glavne kazeinske frakcije u Na-fosfatnom puferu pH 6.5 i temperaturi od 45oC za samo 2 h. Proteolitička aktivnost soja BGRA43 inhibirana je inhibitorom serinskih proteaza (PMSF). Oslobadjanje proteinaze soja BGRA43 sa ćelijskog zida nije zavisno od Ca++-jona. Na osnovu DNK-DNK hibridizacija, PCR analize kao i sekvenciranjem kloniranog PCR produkta koji odgovara katalitičkom regionu proteinaze utvrdjen je visok stepen homologije sa ptrH genom iz soja Lactobacillus helveticus CNRZ32. Izolovan je i okarakterisan spontani Prt- derivat soja BGRA43, označen kao BGRA433. Derivat BGRA433 nije hidrolizovao ni jednu kazeinsku frakciju i pokazano je da poseduje deleciju u katalitičkom regionu proteinaznog gena. Ćelije soja BGRA43 preživljavaju u visokom procentu u uslovima  niske pH vrednosti kao i u prisustvu 0.3% žučnih soli. Humano poreklo, široki spektar antimikrobijalnog delovanja i sposobnost preživljavanja u uslovima koji vladaju u gastrointestinalnom traktu čine soj BGRA43 potencijalnim probiotikom. Lactobacillus helveticus BGRA43 je uspešno iskorišćen i kao starter kultura za proizvodnju jogurta i kiselog mleka.
AB  - Natural isolate BGRA43 was isolated from the human intestinal tract. Using the microbiology and molecular biology tests this thermophilic human isolate was determined as Lactobacillus helveticus. The strain BGRA43 was transformed with plasmid pA1-6, but efficiency of transformation was very low. It was found that BGRA43 contains only one small plasmid pRA1 about 2.4 kb. The strain BGRA43 grow very fast in milk (6h, pH 4.5) as well as in MRS (A600nm, 0.858, 10h). Growth of Lactobacillus helveticus BGRA43 in non-fat skim milk after 6 h at 37oC resulted in a lowering of the pH value to 4.53. Besides the fast acidification, the strain generated a high viscosity of skim milk. The strain BGRA43 produced antagonistic substances against both Gram-positive and Gram-negative bacteria and also exhibited an inhibitory effect on the growth of Clostridium sporogenes. Determination of caseinolytic activity done under optimal conditions (pH 6.5 and 45oC), revealed that proteinase from this strain completely hydrolysed all three casein fractions. The proteolytic activity of whole cells was inhibited by serine proteinase inhibitor (PMSF), suggesting that BGRA43 strain produce serine-type of proteinase. In addition, release of the proteinase from the cell envelope of strain BGRA43 is not Ca++-dependent. DNA-DNA hybridisation, PCR analysis and analysis of subcloning PCR fragments from catalytic region of proteinase showed high similarity (98.9% identity) with prtH gene isolated from Lactobacillus helveticus CNRZ32. Spontaneous Prt- derivative BGRA433 was isolated. This derivative is not able to  hydrolyse any of casein fractions and  hybridisation and PCR analysis showed deletion in catalytic region of proteinase gene. Human origin, antimicrobial activity and surviving at low pH and in presence of bile salts are all properties that consider this strain to be a potential probiotic. Finally, it was demonstrated that the strain BGRA43 can be very attractive as starter culture for production of fermented milk product.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Karakterizacija prirodnog izolata Lactobacillus sp. BGRA43 kao potencijalnog probiotika
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11423
ER  - 

@phdthesis{
author = "Strahinić, Ivana",
year = "2013",
abstract = "Prirodni izolat BGRA43, izolovan iz gastrointestinalnog trakta čoveka, determinisan je na osnovu klasičnih mikrobioloških i molekularno-bioloških metoda kao vrsta Lactobacillus helveticus. Soj poseduje jedan plazmid (pRA1), veličine 2.4 kb, koji se stabilno održava u ćeliji i nakon 10 sukcesivnih presejavanja. Za potrebe genetičkih manipulacija soj je uspešno transformisan plazmidom pA1-6, mada je efikasnost transformacije niska. Optimalna temperatura za rast ovog soja je 42oC, pri čemu je pokazano da soj dobro raste kako u MRS-u tako i u 10% obranom mleku. Nakon 6 sati rasta  u 10% obranom mleku na temperaturi od 37oC dolazi do obaranja pH vrednosti u mleku na 4.53 pri čemu takodje dolazi do formiranja homogenog gruša visokog stepena viskoznosti. Soj BGRA43 pokazuje antimikrobijalnu aktivnost na veći broj Gram-pozitivnih i Gram-negativnih bakterija. Takodje je utvrdjeno da soj BGRA43 poseduje ekstracelularnu proteinazu. Cele ćelije ovog soja hidrolizuju sve tri glavne kazeinske frakcije u Na-fosfatnom puferu pH 6.5 i temperaturi od 45oC za samo 2 h. Proteolitička aktivnost soja BGRA43 inhibirana je inhibitorom serinskih proteaza (PMSF). Oslobadjanje proteinaze soja BGRA43 sa ćelijskog zida nije zavisno od Ca++-jona. Na osnovu DNK-DNK hibridizacija, PCR analize kao i sekvenciranjem kloniranog PCR produkta koji odgovara katalitičkom regionu proteinaze utvrdjen je visok stepen homologije sa ptrH genom iz soja Lactobacillus helveticus CNRZ32. Izolovan je i okarakterisan spontani Prt- derivat soja BGRA43, označen kao BGRA433. Derivat BGRA433 nije hidrolizovao ni jednu kazeinsku frakciju i pokazano je da poseduje deleciju u katalitičkom regionu proteinaznog gena. Ćelije soja BGRA43 preživljavaju u visokom procentu u uslovima  niske pH vrednosti kao i u prisustvu 0.3% žučnih soli. Humano poreklo, široki spektar antimikrobijalnog delovanja i sposobnost preživljavanja u uslovima koji vladaju u gastrointestinalnom traktu čine soj BGRA43 potencijalnim probiotikom. Lactobacillus helveticus BGRA43 je uspešno iskorišćen i kao starter kultura za proizvodnju jogurta i kiselog mleka., Natural isolate BGRA43 was isolated from the human intestinal tract. Using the microbiology and molecular biology tests this thermophilic human isolate was determined as Lactobacillus helveticus. The strain BGRA43 was transformed with plasmid pA1-6, but efficiency of transformation was very low. It was found that BGRA43 contains only one small plasmid pRA1 about 2.4 kb. The strain BGRA43 grow very fast in milk (6h, pH 4.5) as well as in MRS (A600nm, 0.858, 10h). Growth of Lactobacillus helveticus BGRA43 in non-fat skim milk after 6 h at 37oC resulted in a lowering of the pH value to 4.53. Besides the fast acidification, the strain generated a high viscosity of skim milk. The strain BGRA43 produced antagonistic substances against both Gram-positive and Gram-negative bacteria and also exhibited an inhibitory effect on the growth of Clostridium sporogenes. Determination of caseinolytic activity done under optimal conditions (pH 6.5 and 45oC), revealed that proteinase from this strain completely hydrolysed all three casein fractions. The proteolytic activity of whole cells was inhibited by serine proteinase inhibitor (PMSF), suggesting that BGRA43 strain produce serine-type of proteinase. In addition, release of the proteinase from the cell envelope of strain BGRA43 is not Ca++-dependent. DNA-DNA hybridisation, PCR analysis and analysis of subcloning PCR fragments from catalytic region of proteinase showed high similarity (98.9% identity) with prtH gene isolated from Lactobacillus helveticus CNRZ32. Spontaneous Prt- derivative BGRA433 was isolated. This derivative is not able to  hydrolyse any of casein fractions and  hybridisation and PCR analysis showed deletion in catalytic region of proteinase gene. Human origin, antimicrobial activity and surviving at low pH and in presence of bile salts are all properties that consider this strain to be a potential probiotic. Finally, it was demonstrated that the strain BGRA43 can be very attractive as starter culture for production of fermented milk product.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Karakterizacija prirodnog izolata Lactobacillus sp. BGRA43 kao potencijalnog probiotika",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11423"
}

Strahinić, I.. (2013). Karakterizacija prirodnog izolata Lactobacillus sp. BGRA43 kao potencijalnog probiotika. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_11423

Strahinić I. Karakterizacija prirodnog izolata Lactobacillus sp. BGRA43 kao potencijalnog probiotika. 2013;.
https://hdl.handle.net/21.15107/rcub_nardus_11423 .

Strahinić, Ivana, "Karakterizacija prirodnog izolata Lactobacillus sp. BGRA43 kao potencijalnog probiotika" (2013),
https://hdl.handle.net/21.15107/rcub_nardus_11423 .

TY  - JOUR
AU  - Strahinić, Ivana
AU  - Lozo, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Fira, Đorđe
AU  - Kojić, Milan
AU  - Golić, Nataša
AU  - Begović, Jelena
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/632
AB  - Lactobacillus helveticus BGRA43 is a human intestinal isolate showing antimicrobial activity, amongst others, against Yersinia enterocolitica, Shigella sonnei, Shigella flexneri, and Streptococcus pneumoniae. BGRA43 produces PrtH proteinase with proteolytic activity on both casein and beta-lactoglobulin (BLG). BGRA43 is able to reduce the allergenicity of BLG. Bioactive peptides released in BGRA43 fermented milk are potent modulators of innate immunity by modulating the production of proinflammatory cytokines IL-6 and TNF-alpha. BGRA43 is able to survive in simulated gastric and intestinal conditions. The growth of BGRA43 in milk results in a fast acidification lowering the milk pH to 4.53 generating mild, homogeneous, and viscous yogurt-like product. The strain BGRA43 grows suitably in pure cow or goat's milk as well as in milk containing inulin or nutrim even when they are used as the sole carbon source. It is suggested that strain BGRA43 could be used as a single-strain culture for the preparation of yogurt-like products from bovine or caprine milk. Overall, L. helveticus BGRA43 could be considered as a potential probiotic candidate with appropriate technological properties attractive for the dairy industry.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus
VL  - 4
DO  - 10.3389/fmicb.2013.00002
ER  - 

@article{
author = "Strahinić, Ivana and Lozo, Jelena and Terzić-Vidojević, Amarela and Fira, Đorđe and Kojić, Milan and Golić, Nataša and Begović, Jelena and Topisirović, Ljubiša",
year = "2013",
abstract = "Lactobacillus helveticus BGRA43 is a human intestinal isolate showing antimicrobial activity, amongst others, against Yersinia enterocolitica, Shigella sonnei, Shigella flexneri, and Streptococcus pneumoniae. BGRA43 produces PrtH proteinase with proteolytic activity on both casein and beta-lactoglobulin (BLG). BGRA43 is able to reduce the allergenicity of BLG. Bioactive peptides released in BGRA43 fermented milk are potent modulators of innate immunity by modulating the production of proinflammatory cytokines IL-6 and TNF-alpha. BGRA43 is able to survive in simulated gastric and intestinal conditions. The growth of BGRA43 in milk results in a fast acidification lowering the milk pH to 4.53 generating mild, homogeneous, and viscous yogurt-like product. The strain BGRA43 grows suitably in pure cow or goat's milk as well as in milk containing inulin or nutrim even when they are used as the sole carbon source. It is suggested that strain BGRA43 could be used as a single-strain culture for the preparation of yogurt-like products from bovine or caprine milk. Overall, L. helveticus BGRA43 could be considered as a potential probiotic candidate with appropriate technological properties attractive for the dairy industry.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus",
volume = "4",
doi = "10.3389/fmicb.2013.00002"
}

Strahinić, I., Lozo, J., Terzić-Vidojević, A., Fira, Đ., Kojić, M., Golić, N., Begović, J.,& Topisirović, L.. (2013). Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 4.
https://doi.org/10.3389/fmicb.2013.00002

Strahinić I, Lozo J, Terzić-Vidojević A, Fira Đ, Kojić M, Golić N, Begović J, Topisirović L. Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus. in Frontiers in Microbiology. 2013;4.
doi:10.3389/fmicb.2013.00002 .

Strahinić, Ivana, Lozo, Jelena, Terzić-Vidojević, Amarela, Fira, Đorđe, Kojić, Milan, Golić, Nataša, Begović, Jelena, Topisirović, Ljubiša, "Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus" in Frontiers in Microbiology, 4 (2013),
https://doi.org/10.3389/fmicb.2013.00002 . .

TY  - JOUR
AU  - Strahinić, Ivana
AU  - Lukić, Jovanka
AU  - Terzić-Vidojević, Amarela
AU  - Lozo, Jelena
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/669
AB  - Lactobacillus helveticus BGRA43 isolated from human intestines shows antimicrobial activity against foodborne pathogens and during fermentation in milk releases peptides with demonstrated anti-inflammatory properties. In this study, it was found that strain BGRA43 exhibits antimicrobial activity against human pathogens Yersinia enterocolitica, Shigella sonnei, S. flexneri and Streptococcus pneumoniae. Strain BGRA43 was able to survive in simulated gastric juice containing milk and retained cell number stability during the incubation in simulated intestinal conditions. In addition, LC/MS/MS analysis showed the ability of BGRA43 to hydrolyze beta-lactoglobulin. Abundant growth of strain BGRA43 occurred in the presence of prebiotics inulin or concentrated oat bran beta-glucan (Nutrim (R)), even when used as the sole carbon. source. Similarly, strain BGRA43 grew satisfactorily in pure cow's or goat's milk as well as in the milk containing inulin or Nutrim (R). Using the probiotic strain BGRA43 as a single starter strain, fermented milk products obtained from cow's or goat's milk with or without inulin or Nutrim (R) contained about 10(7) CFU/mL. The products were homogeneous and viscous and the best sensory scores were observed for fermented milk beverage made from reconstituted skimmed milk, whole cow's milk and whole goat's milk supplemented with 1 % inulin.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Use of Lactobacillus helveticus BGRA43 for Manufacturing Fermented Milk Products
EP  - 265
IS  - 2
SP  - 257
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_imagine_669
ER  - 

@article{
author = "Strahinić, Ivana and Lukić, Jovanka and Terzić-Vidojević, Amarela and Lozo, Jelena and Kojić, Milan and Topisirović, Ljubiša",
year = "2013",
abstract = "Lactobacillus helveticus BGRA43 isolated from human intestines shows antimicrobial activity against foodborne pathogens and during fermentation in milk releases peptides with demonstrated anti-inflammatory properties. In this study, it was found that strain BGRA43 exhibits antimicrobial activity against human pathogens Yersinia enterocolitica, Shigella sonnei, S. flexneri and Streptococcus pneumoniae. Strain BGRA43 was able to survive in simulated gastric juice containing milk and retained cell number stability during the incubation in simulated intestinal conditions. In addition, LC/MS/MS analysis showed the ability of BGRA43 to hydrolyze beta-lactoglobulin. Abundant growth of strain BGRA43 occurred in the presence of prebiotics inulin or concentrated oat bran beta-glucan (Nutrim (R)), even when used as the sole carbon. source. Similarly, strain BGRA43 grew satisfactorily in pure cow's or goat's milk as well as in the milk containing inulin or Nutrim (R). Using the probiotic strain BGRA43 as a single starter strain, fermented milk products obtained from cow's or goat's milk with or without inulin or Nutrim (R) contained about 10(7) CFU/mL. The products were homogeneous and viscous and the best sensory scores were observed for fermented milk beverage made from reconstituted skimmed milk, whole cow's milk and whole goat's milk supplemented with 1 % inulin.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Use of Lactobacillus helveticus BGRA43 for Manufacturing Fermented Milk Products",
pages = "265-257",
number = "2",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_imagine_669"
}

Strahinić, I., Lukić, J., Terzić-Vidojević, A., Lozo, J., Kojić, M.,& Topisirović, L.. (2013). Use of Lactobacillus helveticus BGRA43 for Manufacturing Fermented Milk Products. in Food Technology and Biotechnology
University of Zagreb., 51(2), 257-265.
https://hdl.handle.net/21.15107/rcub_imagine_669

Strahinić I, Lukić J, Terzić-Vidojević A, Lozo J, Kojić M, Topisirović L. Use of Lactobacillus helveticus BGRA43 for Manufacturing Fermented Milk Products. in Food Technology and Biotechnology. 2013;51(2):257-265.
https://hdl.handle.net/21.15107/rcub_imagine_669 .

Strahinić, Ivana, Lukić, Jovanka, Terzić-Vidojević, Amarela, Lozo, Jelena, Kojić, Milan, Topisirović, Ljubiša, "Use of Lactobacillus helveticus BGRA43 for Manufacturing Fermented Milk Products" in Food Technology and Biotechnology, 51, no. 2 (2013):257-265,
https://hdl.handle.net/21.15107/rcub_imagine_669 .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Strahinić, Ivana
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
AU  - Begović, Jelena
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/594
AB  - Adhesion of bacteria to mucosal surfaces and epithelial cells is one of the key features for the selection of probiotics. In this study, we assessed the adhesion property of Lactococcus lactis subsp. lactis BGKP1 based on its strong autoaggregation phenotype and the presence of the mucin binding protein (MbpL). Genes involved in aggregation (aggL) and possible interaction with mucin (mbpL), present on the same plasmid pKP1, were previously separately cloned in the plasmid pAZIL. In vivo and in vitro experiments revealed potentially different physiological roles of these two proteins in the process of adherence to the intestine during the passage of the strain through the gastrointestinal tract. We correlated the in vitro and in vivo aggregation of the BGKP1-20 carrying plasmid with aggL to binding to the colonic mucus through nonspecific hydrophobic interactions. The expression of AggL on the bacterial cell surface significantly increased the hydrophobicity of the strain. On the other hand, the presence of AggL in the strain reduced its ability to adhere to the ileum. Moreover, MbpL protein showed an affinity to bind gastric type mucin proteins such as MUC5AC. This protein did not contribute to the binding of the strain to the ileal or colonic part of the intestine. Different potential functions of lactococcal AggL and MbpL proteins in the process of adhesion to the gastrointestinal tract are proposed.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa
EP  - 8000
IS  - 22
SP  - 7993
VL  - 78
DO  - 10.1128/AEM.02141-12
ER  - 

@article{
author = "Lukić, Jovanka and Strahinić, Ivana and Jovčić, Branko and Filipić, Brankica and Topisirović, Ljubiša and Kojić, Milan and Begović, Jelena",
year = "2012",
abstract = "Adhesion of bacteria to mucosal surfaces and epithelial cells is one of the key features for the selection of probiotics. In this study, we assessed the adhesion property of Lactococcus lactis subsp. lactis BGKP1 based on its strong autoaggregation phenotype and the presence of the mucin binding protein (MbpL). Genes involved in aggregation (aggL) and possible interaction with mucin (mbpL), present on the same plasmid pKP1, were previously separately cloned in the plasmid pAZIL. In vivo and in vitro experiments revealed potentially different physiological roles of these two proteins in the process of adherence to the intestine during the passage of the strain through the gastrointestinal tract. We correlated the in vitro and in vivo aggregation of the BGKP1-20 carrying plasmid with aggL to binding to the colonic mucus through nonspecific hydrophobic interactions. The expression of AggL on the bacterial cell surface significantly increased the hydrophobicity of the strain. On the other hand, the presence of AggL in the strain reduced its ability to adhere to the ileum. Moreover, MbpL protein showed an affinity to bind gastric type mucin proteins such as MUC5AC. This protein did not contribute to the binding of the strain to the ileal or colonic part of the intestine. Different potential functions of lactococcal AggL and MbpL proteins in the process of adhesion to the gastrointestinal tract are proposed.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa",
pages = "8000-7993",
number = "22",
volume = "78",
doi = "10.1128/AEM.02141-12"
}

Lukić, J., Strahinić, I., Jovčić, B., Filipić, B., Topisirović, L., Kojić, M.,& Begović, J.. (2012). Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 78(22), 7993-8000.
https://doi.org/10.1128/AEM.02141-12

Lukić J, Strahinić I, Jovčić B, Filipić B, Topisirović L, Kojić M, Begović J. Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa. in Applied and Environmental Microbiology. 2012;78(22):7993-8000.
doi:10.1128/AEM.02141-12 .

Lukić, Jovanka, Strahinić, Ivana, Jovčić, Branko, Filipić, Brankica, Topisirović, Ljubiša, Kojić, Milan, Begović, Jelena, "Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa" in Applied and Environmental Microbiology, 78, no. 22 (2012):7993-8000,
https://doi.org/10.1128/AEM.02141-12 . .

TY  - JOUR
AU  - Živković, Milica
AU  - Lopez, Patricia
AU  - Strahinić, Ivana
AU  - Suarez, Ana
AU  - Kojić, Milan
AU  - Fernandez-Garcia, Maria
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
AU  - Ruas-Madiedo, Patricia
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/568
AB  - Traditional fermented foods are the best source for the isolation of strains with specific traits to act as functional starters and to keep the biodiversity of the culture collections. Besides, these strains could be used in the formulation of foods claimed to promote health benefits, i.e. those containing probiotic microorganisms. For the rational selection of strains acting as probiotics, several in vitro tests have been proposed. In the current study, we have characterized the probiotic potential of the strain Lactobacillus paraplanta rum BGCG11, isolated from a Serbian soft, white, homemade cheese, which is able to produce a "ropy" exopolysaccharide (EPS). Three novobiocin derivative strains, which have lost the ropy phenotype, were characterized as well in order to determine the putative role of the EPS in the probiotic potential. Under chemically gastrointestinal conditions, all strains were able to survive around 1-2% (10(6)-10(7) cfu/ml cultivable bacteria) only when they were included in a food matrix (1% skimmed milk). The strains were more resistant to acid conditions than to bile salts and gastric or pancreatic enzymes, which could be due to a pre-adaptation of the parental strain to acidic conditions in the cheese habitat. The ropy EPS did not improve the survival of the producing strain. On the contrary, the presence of an EPS layer surrounding the strain BGCG11 hindered its adhesion to the three epithelial intestinal cell lines tested, since the adhesion of the three non-ropy derivatives was higher than the parental one and also than that of the reference strain Lactobacillus rhamnosus CC. Aiming to propose a potential target application of these strains as probiotics, the cytokine production of peripheral blood mononuclear cells (PBMC) was analyzed. The EPS-producing L paraplantarum BGCG11 strain showed an anti-inflammatory or immunosuppressor profile whereas the non-ropy derivative strains induced higher pro-inflammatory response. In addition, when PBMC were stimulated with increasing concentrations of the purified ropy EPS (1, 10 and 100 mu g/ml) the cytokine profile was similar to that obtained with the EPS-producing lactobacilli, therefore pointing to a putative role of this biopolymer in its immune response.
PB  - Elsevier, Amsterdam
T2  - International Journal of Food Microbiology
T1  - Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics
EP  - 162
IS  - 2
SP  - 155
VL  - 158
DO  - 10.1016/j.ijfoodmicro.2012.07.015
ER  - 

@article{
author = "Živković, Milica and Lopez, Patricia and Strahinić, Ivana and Suarez, Ana and Kojić, Milan and Fernandez-Garcia, Maria and Topisirović, Ljubiša and Golić, Nataša and Ruas-Madiedo, Patricia",
year = "2012",
abstract = "Traditional fermented foods are the best source for the isolation of strains with specific traits to act as functional starters and to keep the biodiversity of the culture collections. Besides, these strains could be used in the formulation of foods claimed to promote health benefits, i.e. those containing probiotic microorganisms. For the rational selection of strains acting as probiotics, several in vitro tests have been proposed. In the current study, we have characterized the probiotic potential of the strain Lactobacillus paraplanta rum BGCG11, isolated from a Serbian soft, white, homemade cheese, which is able to produce a "ropy" exopolysaccharide (EPS). Three novobiocin derivative strains, which have lost the ropy phenotype, were characterized as well in order to determine the putative role of the EPS in the probiotic potential. Under chemically gastrointestinal conditions, all strains were able to survive around 1-2% (10(6)-10(7) cfu/ml cultivable bacteria) only when they were included in a food matrix (1% skimmed milk). The strains were more resistant to acid conditions than to bile salts and gastric or pancreatic enzymes, which could be due to a pre-adaptation of the parental strain to acidic conditions in the cheese habitat. The ropy EPS did not improve the survival of the producing strain. On the contrary, the presence of an EPS layer surrounding the strain BGCG11 hindered its adhesion to the three epithelial intestinal cell lines tested, since the adhesion of the three non-ropy derivatives was higher than the parental one and also than that of the reference strain Lactobacillus rhamnosus CC. Aiming to propose a potential target application of these strains as probiotics, the cytokine production of peripheral blood mononuclear cells (PBMC) was analyzed. The EPS-producing L paraplantarum BGCG11 strain showed an anti-inflammatory or immunosuppressor profile whereas the non-ropy derivative strains induced higher pro-inflammatory response. In addition, when PBMC were stimulated with increasing concentrations of the purified ropy EPS (1, 10 and 100 mu g/ml) the cytokine profile was similar to that obtained with the EPS-producing lactobacilli, therefore pointing to a putative role of this biopolymer in its immune response.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Food Microbiology",
title = "Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics",
pages = "162-155",
number = "2",
volume = "158",
doi = "10.1016/j.ijfoodmicro.2012.07.015"
}

Živković, M., Lopez, P., Strahinić, I., Suarez, A., Kojić, M., Fernandez-Garcia, M., Topisirović, L., Golić, N.,& Ruas-Madiedo, P.. (2012). Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics. in International Journal of Food Microbiology
Elsevier, Amsterdam., 158(2), 155-162.
https://doi.org/10.1016/j.ijfoodmicro.2012.07.015

Živković M, Lopez P, Strahinić I, Suarez A, Kojić M, Fernandez-Garcia M, Topisirović L, Golić N, Ruas-Madiedo P. Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics. in International Journal of Food Microbiology. 2012;158(2):155-162.
doi:10.1016/j.ijfoodmicro.2012.07.015 .

Živković, Milica, Lopez, Patricia, Strahinić, Ivana, Suarez, Ana, Kojić, Milan, Fernandez-Garcia, Maria, Topisirović, Ljubiša, Golić, Nataša, Ruas-Madiedo, Patricia, "Characterisation of the exopolysaccharide (EPS)-producing Lactobacillus paraplantarum BGCG11 and its non-EPS producing derivative strains as potential probiotics" in International Journal of Food Microbiology, 158, no. 2 (2012):155-162,
https://doi.org/10.1016/j.ijfoodmicro.2012.07.015 . .