TY  - JOUR
AU  - Stanković, Mia
AU  - Škaro Bogojević, Sanja
AU  - Kljun, Jakob
AU  - Milanović, Žiko
AU  - Stevanović, Nevena
AU  - Lazić, Jelena
AU  - Vojnović, Sandra
AU  - Turel, Iztok
AU  - Đuran, Miloš
AU  - Glišić, Biljana
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0162013424000953
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2368
AB  - Recognizing that metal ions play an important role in modifying the pharmacological properties of known organic-based drugs, the present manuscript addresses the complexation of the antifungal agent voriconazole (vcz) with the biologically relevant silver(I) ion as a strategy for the development of new antimycotics. The synthesized silver(I) complexes with vcz were characterized by mass spectrometry, IR, UV–Vis and NMR spectroscopy and single-crystal X-ray diffraction analysis. The crystallographic results showed that complexes {[Ag(vcz)(H2O)]CH3SO3}n (1), {[Ag(vcz)2]BF4}n (2) and {[Ag(vcz)2]PF6}n (3) have polymeric structures in the solid state, in which silver(I) ions have a distorted tetrahedral geometry. On the other hand, DFT calculations revealed that the investigated silver(I) complexes 1–3 in DMSO exist as linear [Ag(vcz-N2)(vcz-N19)]+ (1a), [Ag(vcz-N2)(vcz-N4)]+ (2a) and [Ag(vcz-N4)2]+ (3a) species, respectively. The evaluated complexes showed an enhanced anti-Candida activity compared to the parent drug with minimal inhibitory concentration (MIC) values in the range of 0.02–1.05 μM. In comparison with vcz, the corresponding silver(I) complexes showed better activity in prevention hyphae and biofilm formation of C. albicans, indicating that they could be considered as promising agents against Candida that significantly inhibit its virulence. Also, these complexes are much better inhibitors of ergosterol synthesis in the cell membrane of C. albicans at the concentration of 0.5 × MIC. This is also confirmed by a molecular docking, which revealed that complexes 1a – 3a showed better inhibitory activity than vcz against the sterol 14α-demethylase enzyme cytochrome P450 (CYP51B), which plays a crucial role in the formation of ergosterol.
PB  - Elsevier
T2  - Journal of Inorganic Biochemistry
T1  - Silver(I) complexes with voriconazole as promising anti-Candida agents
SP  - 112572
VL  - 256
DO  - 10.1016/j.jinorgbio.2024.112572
ER  - 

@article{
author = "Stanković, Mia and Škaro Bogojević, Sanja and Kljun, Jakob and Milanović, Žiko and Stevanović, Nevena and Lazić, Jelena and Vojnović, Sandra and Turel, Iztok and Đuran, Miloš and Glišić, Biljana",
year = "2024",
abstract = "Recognizing that metal ions play an important role in modifying the pharmacological properties of known organic-based drugs, the present manuscript addresses the complexation of the antifungal agent voriconazole (vcz) with the biologically relevant silver(I) ion as a strategy for the development of new antimycotics. The synthesized silver(I) complexes with vcz were characterized by mass spectrometry, IR, UV–Vis and NMR spectroscopy and single-crystal X-ray diffraction analysis. The crystallographic results showed that complexes {[Ag(vcz)(H2O)]CH3SO3}n (1), {[Ag(vcz)2]BF4}n (2) and {[Ag(vcz)2]PF6}n (3) have polymeric structures in the solid state, in which silver(I) ions have a distorted tetrahedral geometry. On the other hand, DFT calculations revealed that the investigated silver(I) complexes 1–3 in DMSO exist as linear [Ag(vcz-N2)(vcz-N19)]+ (1a), [Ag(vcz-N2)(vcz-N4)]+ (2a) and [Ag(vcz-N4)2]+ (3a) species, respectively. The evaluated complexes showed an enhanced anti-Candida activity compared to the parent drug with minimal inhibitory concentration (MIC) values in the range of 0.02–1.05 μM. In comparison with vcz, the corresponding silver(I) complexes showed better activity in prevention hyphae and biofilm formation of C. albicans, indicating that they could be considered as promising agents against Candida that significantly inhibit its virulence. Also, these complexes are much better inhibitors of ergosterol synthesis in the cell membrane of C. albicans at the concentration of 0.5 × MIC. This is also confirmed by a molecular docking, which revealed that complexes 1a – 3a showed better inhibitory activity than vcz against the sterol 14α-demethylase enzyme cytochrome P450 (CYP51B), which plays a crucial role in the formation of ergosterol.",
publisher = "Elsevier",
journal = "Journal of Inorganic Biochemistry",
title = "Silver(I) complexes with voriconazole as promising anti-Candida agents",
pages = "112572",
volume = "256",
doi = "10.1016/j.jinorgbio.2024.112572"
}

Stanković, M., Škaro Bogojević, S., Kljun, J., Milanović, Ž., Stevanović, N., Lazić, J., Vojnović, S., Turel, I., Đuran, M.,& Glišić, B.. (2024). Silver(I) complexes with voriconazole as promising anti-Candida agents. in Journal of Inorganic Biochemistry
Elsevier., 256, 112572.
https://doi.org/10.1016/j.jinorgbio.2024.112572

Stanković M, Škaro Bogojević S, Kljun J, Milanović Ž, Stevanović N, Lazić J, Vojnović S, Turel I, Đuran M, Glišić B. Silver(I) complexes with voriconazole as promising anti-Candida agents. in Journal of Inorganic Biochemistry. 2024;256:112572.
doi:10.1016/j.jinorgbio.2024.112572 .

Stanković, Mia, Škaro Bogojević, Sanja, Kljun, Jakob, Milanović, Žiko, Stevanović, Nevena, Lazić, Jelena, Vojnović, Sandra, Turel, Iztok, Đuran, Miloš, Glišić, Biljana, "Silver(I) complexes with voriconazole as promising anti-Candida agents" in Journal of Inorganic Biochemistry, 256 (2024):112572,
https://doi.org/10.1016/j.jinorgbio.2024.112572 . .

TY  - JOUR
AU  - Stanković, Mia
AU  - Kljun, Jakob
AU  - Stevanović, Nevena Lj.
AU  - Lazić, Jelena
AU  - Škaro Bogojević, Sanja
AU  - Vojnović, Sandra
AU  - Zlatar, Matija
AU  - Nikodinović-Runić, Jasmina
AU  - Turel, Iztok
AU  - Đuran, Miloš
AU  - Glišić, Biljana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2303
AB  - Inspired by the emergence of resistance to currently available antifungal therapy and by the great potential of metal complexes for the treatment of various diseases, we synthesized three new silver(I) complexes containing clinically used antifungal azoles as ligands, [Ag(ecz)2]SbF6 (1, ecz is econazole), {[Ag(vcz)2]SbF6}n (2, vcz is voriconazole), and [Ag(ctz)2]SbF6 (3, ctz is clotrimazole), and investigated their antimicrobial properties. The synthesized complexes were characterized by mass spectrometry, IR, UV-vis and 1H NMR spectroscopy, cyclic voltammetry, and single-crystal X-ray diffraction analysis. In the mononuclear complexes 1 and 3 with ecz and ctz, respectively, the silver(I) ion has the expected linear geometry, in which the azoles are monodentately coordinated to this metal center through the N3 imidazole nitrogen atom. In contrast, the vcz-containing complex 2 has a polymeric structure in the solid state in which the silver(I) ions are coordinated by four nitrogen atoms in a distorted tetrahedral geometry. DFT calculations were done to predict the most favorable structures of the studied complexes in DMSO solution. All the studied silver(I) complexes have shown excellent antifungal and good to moderate antibacterial activities with minimal inhibitory concentration (MIC) values in the ranges of 0.01–27.1 and 2.61–47.9 μM on the selected panel of fungi and bacteria, respectively. Importantly, the complexes 1–3 have exhibited a significantly improved antifungal activity compared to the free azoles, with the most pronounced effect observed in the case of complex 2 compared to the parent vcz against Candida glabrata with an increase of activity by five orders of magnitude. Moreover, the silver(I)-azole complexes 2 and 3 significantly inhibited the formation of C. albicans hyphae and biofilms at the subinhibitory concentration of 50% MIC. To investigate the impact of the complex 3 more thoroughly on Candida pathogenesis, its effect on the adherence of C. albicans to A549 cells (human adenocarcinoma alveolar basal epithelial cells), as an initial step of the invasion of host cells, was studied.
PB  - Royal Society of Chemistry (RSC)
T2  - Dalton Transactions
T1  - Silver(I) complexes containing antifungal azoles: significant improvement of the anti-Candida potential of the azole drug after its coordination to the silver(I) ion
DO  - 10.1039/D3DT03010E
ER  - 

@article{
author = "Stanković, Mia and Kljun, Jakob and Stevanović, Nevena Lj. and Lazić, Jelena and Škaro Bogojević, Sanja and Vojnović, Sandra and Zlatar, Matija and Nikodinović-Runić, Jasmina and Turel, Iztok and Đuran, Miloš and Glišić, Biljana",
year = "2024",
abstract = "Inspired by the emergence of resistance to currently available antifungal therapy and by the great potential of metal complexes for the treatment of various diseases, we synthesized three new silver(I) complexes containing clinically used antifungal azoles as ligands, [Ag(ecz)2]SbF6 (1, ecz is econazole), {[Ag(vcz)2]SbF6}n (2, vcz is voriconazole), and [Ag(ctz)2]SbF6 (3, ctz is clotrimazole), and investigated their antimicrobial properties. The synthesized complexes were characterized by mass spectrometry, IR, UV-vis and 1H NMR spectroscopy, cyclic voltammetry, and single-crystal X-ray diffraction analysis. In the mononuclear complexes 1 and 3 with ecz and ctz, respectively, the silver(I) ion has the expected linear geometry, in which the azoles are monodentately coordinated to this metal center through the N3 imidazole nitrogen atom. In contrast, the vcz-containing complex 2 has a polymeric structure in the solid state in which the silver(I) ions are coordinated by four nitrogen atoms in a distorted tetrahedral geometry. DFT calculations were done to predict the most favorable structures of the studied complexes in DMSO solution. All the studied silver(I) complexes have shown excellent antifungal and good to moderate antibacterial activities with minimal inhibitory concentration (MIC) values in the ranges of 0.01–27.1 and 2.61–47.9 μM on the selected panel of fungi and bacteria, respectively. Importantly, the complexes 1–3 have exhibited a significantly improved antifungal activity compared to the free azoles, with the most pronounced effect observed in the case of complex 2 compared to the parent vcz against Candida glabrata with an increase of activity by five orders of magnitude. Moreover, the silver(I)-azole complexes 2 and 3 significantly inhibited the formation of C. albicans hyphae and biofilms at the subinhibitory concentration of 50% MIC. To investigate the impact of the complex 3 more thoroughly on Candida pathogenesis, its effect on the adherence of C. albicans to A549 cells (human adenocarcinoma alveolar basal epithelial cells), as an initial step of the invasion of host cells, was studied.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "Dalton Transactions",
title = "Silver(I) complexes containing antifungal azoles: significant improvement of the anti-Candida potential of the azole drug after its coordination to the silver(I) ion",
doi = "10.1039/D3DT03010E"
}

Stanković, M., Kljun, J., Stevanović, N. Lj., Lazić, J., Škaro Bogojević, S., Vojnović, S., Zlatar, M., Nikodinović-Runić, J., Turel, I., Đuran, M.,& Glišić, B.. (2024). Silver(I) complexes containing antifungal azoles: significant improvement of the anti-Candida potential of the azole drug after its coordination to the silver(I) ion. in Dalton Transactions
Royal Society of Chemistry (RSC)..
https://doi.org/10.1039/D3DT03010E

Stanković M, Kljun J, Stevanović NL, Lazić J, Škaro Bogojević S, Vojnović S, Zlatar M, Nikodinović-Runić J, Turel I, Đuran M, Glišić B. Silver(I) complexes containing antifungal azoles: significant improvement of the anti-Candida potential of the azole drug after its coordination to the silver(I) ion. in Dalton Transactions. 2024;.
doi:10.1039/D3DT03010E .

Stanković, Mia, Kljun, Jakob, Stevanović, Nevena Lj., Lazić, Jelena, Škaro Bogojević, Sanja, Vojnović, Sandra, Zlatar, Matija, Nikodinović-Runić, Jasmina, Turel, Iztok, Đuran, Miloš, Glišić, Biljana, "Silver(I) complexes containing antifungal azoles: significant improvement of the anti-Candida potential of the azole drug after its coordination to the silver(I) ion" in Dalton Transactions (2024),
https://doi.org/10.1039/D3DT03010E . .

TY  - JOUR
AU  - Vojnović, Sandra
AU  - Aleksić, Ivana
AU  - Ilić-Tomić, Tatjana
AU  - Stevanović, Milena
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://doi.org/10.1007/s00253-023-12900-x
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2314
AB  - The application of enzymes is expanding across diverse industries due to their nontoxic and biodegradable characteristics. Another advantage is their cost-effectiveness, reflected in reduced processing time, water, and energy consumption. Although Gram-positive bacteria, Bacillus, and Streptomyces spp. are successfully used for production of industrially relevant enzymes, they still lag far behind Escherichia coli as hosts for recombinant protein production. Generally, proteins secreted by Bacillus and Streptomyces hosts are released into the culture medium; their native conformation is preserved and easier recovery process enabled. Given the resilience of both hosts in harsh environmental conditions and their spore-forming capability, a deeper understanding and broader use of Bacillus and Streptomyces as expression hosts could significantly enhance the robustness of industrial bioprocesses. This mini-review aims to compare two expression hosts, emphasizing their specific advantages in industrial surroundings such are chemical, detergent, textile, food, animal feed, leather, and paper industries. The homologous sources, heterologous hosts, and molecular tools used for the production of recombinant proteins in these hosts are discussed. The potential to use both hosts as biocatalysts is also evaluated. Undoubtedly, Bacillus and Streptomyces spp. as production hosts possess the potential to take on a more substantial role, providing superior (bio-based) process robustness and flexibility.
PB  - Springer Nature
T2  - Applied Microbiology and Biotechnology
T1  - Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes
IS  - 1
SP  - 185
VL  - 108
DO  - 10.1007/s00253-023-12900-x
ER  - 

@article{
author = "Vojnović, Sandra and Aleksić, Ivana and Ilić-Tomić, Tatjana and Stevanović, Milena and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "The application of enzymes is expanding across diverse industries due to their nontoxic and biodegradable characteristics. Another advantage is their cost-effectiveness, reflected in reduced processing time, water, and energy consumption. Although Gram-positive bacteria, Bacillus, and Streptomyces spp. are successfully used for production of industrially relevant enzymes, they still lag far behind Escherichia coli as hosts for recombinant protein production. Generally, proteins secreted by Bacillus and Streptomyces hosts are released into the culture medium; their native conformation is preserved and easier recovery process enabled. Given the resilience of both hosts in harsh environmental conditions and their spore-forming capability, a deeper understanding and broader use of Bacillus and Streptomyces as expression hosts could significantly enhance the robustness of industrial bioprocesses. This mini-review aims to compare two expression hosts, emphasizing their specific advantages in industrial surroundings such are chemical, detergent, textile, food, animal feed, leather, and paper industries. The homologous sources, heterologous hosts, and molecular tools used for the production of recombinant proteins in these hosts are discussed. The potential to use both hosts as biocatalysts is also evaluated. Undoubtedly, Bacillus and Streptomyces spp. as production hosts possess the potential to take on a more substantial role, providing superior (bio-based) process robustness and flexibility.",
publisher = "Springer Nature",
journal = "Applied Microbiology and Biotechnology",
title = "Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes",
number = "1",
pages = "185",
volume = "108",
doi = "10.1007/s00253-023-12900-x"
}

Vojnović, S., Aleksić, I., Ilić-Tomić, T., Stevanović, M.,& Nikodinović-Runić, J.. (2024). Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes. in Applied Microbiology and Biotechnology
Springer Nature., 108(1), 185.
https://doi.org/10.1007/s00253-023-12900-x

Vojnović S, Aleksić I, Ilić-Tomić T, Stevanović M, Nikodinović-Runić J. Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes. in Applied Microbiology and Biotechnology. 2024;108(1):185.
doi:10.1007/s00253-023-12900-x .

Vojnović, Sandra, Aleksić, Ivana, Ilić-Tomić, Tatjana, Stevanović, Milena, Nikodinović-Runić, Jasmina, "Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes" in Applied Microbiology and Biotechnology, 108, no. 1 (2024):185,
https://doi.org/10.1007/s00253-023-12900-x . .

TY  - JOUR
AU  - Andrejević, Tina
AU  - Aleksić, Ivana
AU  - Kljun, Jakob
AU  - Počkaj, Marta
AU  - Zlatar, Matija
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
AU  - Turel, Iztok
AU  - Djuran, Miloš
AU  - Glišić, Biljana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1789
AB  - Dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz) was used as a ligand for the synthesis of new copper(II) and silver(I) complexes, [CuCl2(py-2pz)]2 (1), [Cu(CF3SO3)(H2O)(py-2pz)2]CF3SO3·2H2O (2), [Ag(py-2pz)2]PF6 (3) and {[Ag(NO3)(py-2pz)]·0.5H2O}n (4). The complexes were characterized by spectroscopic and electrochemical methods, while their structures were determined by single crystal X-ray diffraction analysis. The X-ray analysis revealed the bidentate coordination mode of py-2pz to the corresponding metal ion via its pyridine and pyrazine nitrogen atoms in all complexes, while in polynuclear complex 4, the heterocyclic pyrazine ring of one py-2pz additionally behaves as a bridging ligand between two Ag(I) ions. DFT calculations were performed to elucidate the structures of the investigated complexes in solution. The antimicrobial potential of the complexes 1–4 was evaluated against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two Candida (C. albicans and C. parapsilosis) species. Silver(I) complexes 3 and 4 have shown good antibacterial and antifungal properties with minimal inhibitory concentration (MIC) values ranging from 4.9 to 39.0 μM (3.9–31.2 μg mL−1). All complexes inhibited the filamentation of C. albicans and hyphae formation, while silver(I) complexes 3 and 4 had also the ability to inhibit the biofilm formation process of this fungus. The binding affinity of the complexes 1–4 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy to clarify the mode of their antimicrobial activity. Catechol oxidase biomimetic catalytic activity of copper(II) complexes 1 and 2 was additionally investigated by using 3,5-di-tert-butylcatechol (3,5-DTBC) and o-aminophenol (OAP) as substrates.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T2  - RSC Advances
T1  - Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex
EP  - 4393
IS  - 7
SP  - 4376
VL  - 13
DO  - 10.1039/D2RA07401J
ER  - 

@article{
author = "Andrejević, Tina and Aleksić, Ivana and Kljun, Jakob and Počkaj, Marta and Zlatar, Matija and Vojnović, Sandra and Nikodinović-Runić, Jasmina and Turel, Iztok and Djuran, Miloš and Glišić, Biljana",
year = "2023",
abstract = "Dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz) was used as a ligand for the synthesis of new copper(II) and silver(I) complexes, [CuCl2(py-2pz)]2 (1), [Cu(CF3SO3)(H2O)(py-2pz)2]CF3SO3·2H2O (2), [Ag(py-2pz)2]PF6 (3) and {[Ag(NO3)(py-2pz)]·0.5H2O}n (4). The complexes were characterized by spectroscopic and electrochemical methods, while their structures were determined by single crystal X-ray diffraction analysis. The X-ray analysis revealed the bidentate coordination mode of py-2pz to the corresponding metal ion via its pyridine and pyrazine nitrogen atoms in all complexes, while in polynuclear complex 4, the heterocyclic pyrazine ring of one py-2pz additionally behaves as a bridging ligand between two Ag(I) ions. DFT calculations were performed to elucidate the structures of the investigated complexes in solution. The antimicrobial potential of the complexes 1–4 was evaluated against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two Candida (C. albicans and C. parapsilosis) species. Silver(I) complexes 3 and 4 have shown good antibacterial and antifungal properties with minimal inhibitory concentration (MIC) values ranging from 4.9 to 39.0 μM (3.9–31.2 μg mL−1). All complexes inhibited the filamentation of C. albicans and hyphae formation, while silver(I) complexes 3 and 4 had also the ability to inhibit the biofilm formation process of this fungus. The binding affinity of the complexes 1–4 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy to clarify the mode of their antimicrobial activity. Catechol oxidase biomimetic catalytic activity of copper(II) complexes 1 and 2 was additionally investigated by using 3,5-di-tert-butylcatechol (3,5-DTBC) and o-aminophenol (OAP) as substrates.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances, RSC Advances",
title = "Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex",
pages = "4393-4376",
number = "7",
volume = "13",
doi = "10.1039/D2RA07401J"
}

Andrejević, T., Aleksić, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnović, S., Nikodinović-Runić, J., Turel, I., Djuran, M.,& Glišić, B.. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex. in RSC Advances
Royal Society of Chemistry., 13(7), 4376-4393.
https://doi.org/10.1039/D2RA07401J

Andrejević T, Aleksić I, Kljun J, Počkaj M, Zlatar M, Vojnović S, Nikodinović-Runić J, Turel I, Djuran M, Glišić B. Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex. in RSC Advances. 2023;13(7):4376-4393.
doi:10.1039/D2RA07401J .

Andrejević, Tina, Aleksić, Ivana, Kljun, Jakob, Počkaj, Marta, Zlatar, Matija, Vojnović, Sandra, Nikodinović-Runić, Jasmina, Turel, Iztok, Djuran, Miloš, Glišić, Biljana, "Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex" in RSC Advances, 13, no. 7 (2023):4376-4393,
https://doi.org/10.1039/D2RA07401J . .

TY  - JOUR
AU  - Hertrampf, Gesa
AU  - Vojnović, Sandra
AU  - Müller, Jonas I.
AU  - Merten, Christian
AU  - Nikodinović-Runić, Jasmina
AU  - Gulder, Tobias A. M.
PY  - 2023
UR  - https://doi.org/10.1021/acs.joc.3c01285
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2153
AB  - The myxobacterial natural product myxocoumarin A from Stigmatella aurantiaca MYX-030 has remarkable antifungal activity against agriculturally relevant pathogens. To broaden the initial evaluation of its biological potential, we herein completed the first total synthesis of myxocoumarin A. This synthetic access facilitated stereochemical investigations on the natural product structure, revealing its (R)-configuration. Biological activity profiling showed a lack of activity against Candida spp. and Gram-negative bacteria but revealed strong antibiotic activities against Bacillus subtilis and Staphylococcus aureus, including MRSA.
T2  - The Journal of Organic Chemistry
T1  - Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A
EP  - 14188
IS  - 19
SP  - 14184
VL  - 88
DO  - 10.1021/acs.joc.3c01285
ER  - 

@article{
author = "Hertrampf, Gesa and Vojnović, Sandra and Müller, Jonas I. and Merten, Christian and Nikodinović-Runić, Jasmina and Gulder, Tobias A. M.",
year = "2023",
abstract = "The myxobacterial natural product myxocoumarin A from Stigmatella aurantiaca MYX-030 has remarkable antifungal activity against agriculturally relevant pathogens. To broaden the initial evaluation of its biological potential, we herein completed the first total synthesis of myxocoumarin A. This synthetic access facilitated stereochemical investigations on the natural product structure, revealing its (R)-configuration. Biological activity profiling showed a lack of activity against Candida spp. and Gram-negative bacteria but revealed strong antibiotic activities against Bacillus subtilis and Staphylococcus aureus, including MRSA.",
journal = "The Journal of Organic Chemistry",
title = "Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A",
pages = "14188-14184",
number = "19",
volume = "88",
doi = "10.1021/acs.joc.3c01285"
}

Hertrampf, G., Vojnović, S., Müller, J. I., Merten, C., Nikodinović-Runić, J.,& Gulder, T. A. M.. (2023). Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A. in The Journal of Organic Chemistry, 88(19), 14184-14188.
https://doi.org/10.1021/acs.joc.3c01285

Hertrampf G, Vojnović S, Müller JI, Merten C, Nikodinović-Runić J, Gulder TAM. Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A. in The Journal of Organic Chemistry. 2023;88(19):14184-14188.
doi:10.1021/acs.joc.3c01285 .

Hertrampf, Gesa, Vojnović, Sandra, Müller, Jonas I., Merten, Christian, Nikodinović-Runić, Jasmina, Gulder, Tobias A. M., "Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A" in The Journal of Organic Chemistry, 88, no. 19 (2023):14184-14188,
https://doi.org/10.1021/acs.joc.3c01285 . .

TY  - CONF
AU  - Stevanović, Milena
AU  - Pantelić, Brana
AU  - Janković, Vukašin
AU  - Nikodinović-Runić, Jasmina
AU  - Vojnović, Sandra
PY  - 2023
UR  - https://www.fems2023.org/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2022
AB  - Plastic waste has become a serious global challenge that calls for sustainable solutions and requires
rapid actions. Biocatalysis could present an adequate answer to this problem by providing different
enzymes capable of degrading plastic polymers. Streptomyces strains as predominant soil
inhabitants have also adapted to the presence of variety of plastic waste in natural environments,
so they have been examined for the plastic degrading capabilities.
The aim of this work was to improve the biocatalytic properties of Streptomyces strains for their
use in biodegradation of plastic polymers and develop a system for heterologous expression of
polyethylene therephtalate (PET) degrading enzymes in Streptomyces spp.
Well studied Streptomyces lividans TK24 and S. albus NRRL B-1335, as well as two newly
isolated Streptomycetes were used for expression of benchmark PETases and cutinases. Enzymes
were cloned into pGM1202 Escherichia coli–Streptomyces shuttle vector and subsequently
introduced into Streptomyces hosts either by polyethylene glycol-mediated protoplasts
transformation or by electroporation. Cell-free extracts and supernatants of transformed cells were
tested on different plastics using bis(2-hydroxyethyl) terephthalate (BHET), polycaprolactone
(PCL) and Impranil as substrates in plate assays.
Expression of leaf-branch compost cutinase in S. albus and S. lividans resulted in an 8.5- and 2.5-
times increase in esterase activities, respectively. Introduction of the enzyme into newly isolated
strains that already showed some plastic degrading activity resulted in synergistic activity of the
recombinant strains.
C3  - FEMS2023 Congress of European Microbiologists
T1  - Expression of PET-hydrolyzing enzymes in Streptomyces spp.
EP  - 836
SP  - 836
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2022
ER  - 

@conference{
author = "Stevanović, Milena and Pantelić, Brana and Janković, Vukašin and Nikodinović-Runić, Jasmina and Vojnović, Sandra",
year = "2023",
abstract = "Plastic waste has become a serious global challenge that calls for sustainable solutions and requires
rapid actions. Biocatalysis could present an adequate answer to this problem by providing different
enzymes capable of degrading plastic polymers. Streptomyces strains as predominant soil
inhabitants have also adapted to the presence of variety of plastic waste in natural environments,
so they have been examined for the plastic degrading capabilities.
The aim of this work was to improve the biocatalytic properties of Streptomyces strains for their
use in biodegradation of plastic polymers and develop a system for heterologous expression of
polyethylene therephtalate (PET) degrading enzymes in Streptomyces spp.
Well studied Streptomyces lividans TK24 and S. albus NRRL B-1335, as well as two newly
isolated Streptomycetes were used for expression of benchmark PETases and cutinases. Enzymes
were cloned into pGM1202 Escherichia coli–Streptomyces shuttle vector and subsequently
introduced into Streptomyces hosts either by polyethylene glycol-mediated protoplasts
transformation or by electroporation. Cell-free extracts and supernatants of transformed cells were
tested on different plastics using bis(2-hydroxyethyl) terephthalate (BHET), polycaprolactone
(PCL) and Impranil as substrates in plate assays.
Expression of leaf-branch compost cutinase in S. albus and S. lividans resulted in an 8.5- and 2.5-
times increase in esterase activities, respectively. Introduction of the enzyme into newly isolated
strains that already showed some plastic degrading activity resulted in synergistic activity of the
recombinant strains.",
journal = "FEMS2023 Congress of European Microbiologists",
title = "Expression of PET-hydrolyzing enzymes in Streptomyces spp.",
pages = "836-836",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2022"
}

Stevanović, M., Pantelić, B., Janković, V., Nikodinović-Runić, J.,& Vojnović, S.. (2023). Expression of PET-hydrolyzing enzymes in Streptomyces spp.. in FEMS2023 Congress of European Microbiologists, 10, 836-836.
https://hdl.handle.net/21.15107/rcub_imagine_2022

Stevanović M, Pantelić B, Janković V, Nikodinović-Runić J, Vojnović S. Expression of PET-hydrolyzing enzymes in Streptomyces spp.. in FEMS2023 Congress of European Microbiologists. 2023;10:836-836.
https://hdl.handle.net/21.15107/rcub_imagine_2022 .

Stevanović, Milena, Pantelić, Brana, Janković, Vukašin, Nikodinović-Runić, Jasmina, Vojnović, Sandra, "Expression of PET-hydrolyzing enzymes in Streptomyces spp." in FEMS2023 Congress of European Microbiologists, 10 (2023):836-836,
https://hdl.handle.net/21.15107/rcub_imagine_2022 .

TY  - CONF
AU  - Ašanin P., Darko
AU  - Vojnović, Sandra
AU  - Andrejević P., Tina
AU  - Marković R., Violeta
AU  - Perdih, Franc
AU  - Turel, Iztok
AU  - Djuran I., Miloš
AU  - Nikodinović-Runić, Jasmina
AU  - Glišić Đ., Biljana
PY  - 2023
UR  - https://isabc2023.com/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1925
AB  - Cisplatin is one of the most used anticancer agents, and along with carboplatin and
oxaliplatin, is a part of more than 50% of clinically applied anticancer regimens [1]. However,
the side effects of cisplatin are severe and include dose-limiting toxicity, such as neurotoxicity,
nephrotoxicity and ototoxicity. Platinum(II) complexes with different structure from cisplatin
provide many opportunities for design of novel antitumor drugs with improved
pharmacological properties. Considering this, in the present study, new platinum(II) complexes
with phenothiazine (phtz) and N-methylphenothiazine (N-Mephtz), [PtCl2(phtz)(CH3CN)] (1)
and [PtCl2(N-Mephtz)(CH3CN)] (2), were synthesized. These complexes were characterized by
elemental microanalysis, NMR (1H and 13C) and IR spectroscopic measurements, while the
structure of complex 1 was determined by single-crystal X-ray diffraction analysis. The
antitumor activity of the platinum(II) complexes was tested in vitro against a panel of human
cancer cell lines, including A549 (lung cancer), A375 (melanoma, skin cancer), MDA-MB-231
(breast cancer), and HCT116 (colon cancer). To check the selectivity of the synthesized
complexes 1 and 2, a healthy MRC-5 cell line (lung fibroblasts) was also included in this study.
PB  - Greece : University of Ioannina
C3  - 16th International Symposium on Applied Bioinorganic Chemistry
T1  - Structural characterization and antitumor activity of platinum(II) complexes with phenothiazine and N-methylphenothiazine
SP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1925
ER  - 

@conference{
author = "Ašanin P., Darko and Vojnović, Sandra and Andrejević P., Tina and Marković R., Violeta and Perdih, Franc and Turel, Iztok and Djuran I., Miloš and Nikodinović-Runić, Jasmina and Glišić Đ., Biljana",
year = "2023",
abstract = "Cisplatin is one of the most used anticancer agents, and along with carboplatin and
oxaliplatin, is a part of more than 50% of clinically applied anticancer regimens [1]. However,
the side effects of cisplatin are severe and include dose-limiting toxicity, such as neurotoxicity,
nephrotoxicity and ototoxicity. Platinum(II) complexes with different structure from cisplatin
provide many opportunities for design of novel antitumor drugs with improved
pharmacological properties. Considering this, in the present study, new platinum(II) complexes
with phenothiazine (phtz) and N-methylphenothiazine (N-Mephtz), [PtCl2(phtz)(CH3CN)] (1)
and [PtCl2(N-Mephtz)(CH3CN)] (2), were synthesized. These complexes were characterized by
elemental microanalysis, NMR (1H and 13C) and IR spectroscopic measurements, while the
structure of complex 1 was determined by single-crystal X-ray diffraction analysis. The
antitumor activity of the platinum(II) complexes was tested in vitro against a panel of human
cancer cell lines, including A549 (lung cancer), A375 (melanoma, skin cancer), MDA-MB-231
(breast cancer), and HCT116 (colon cancer). To check the selectivity of the synthesized
complexes 1 and 2, a healthy MRC-5 cell line (lung fibroblasts) was also included in this study.",
publisher = "Greece : University of Ioannina",
journal = "16th International Symposium on Applied Bioinorganic Chemistry",
title = "Structural characterization and antitumor activity of platinum(II) complexes with phenothiazine and N-methylphenothiazine",
pages = "195",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1925"
}

Ašanin P., D., Vojnović, S., Andrejević P., T., Marković R., V., Perdih, F., Turel, I., Djuran I., M., Nikodinović-Runić, J.,& Glišić Đ., B.. (2023). Structural characterization and antitumor activity of platinum(II) complexes with phenothiazine and N-methylphenothiazine. in 16th International Symposium on Applied Bioinorganic Chemistry
Greece : University of Ioannina., 195.
https://hdl.handle.net/21.15107/rcub_imagine_1925

Ašanin P. D, Vojnović S, Andrejević P. T, Marković R. V, Perdih F, Turel I, Djuran I. M, Nikodinović-Runić J, Glišić Đ. B. Structural characterization and antitumor activity of platinum(II) complexes with phenothiazine and N-methylphenothiazine. in 16th International Symposium on Applied Bioinorganic Chemistry. 2023;:195.
https://hdl.handle.net/21.15107/rcub_imagine_1925 .

Ašanin P., Darko, Vojnović, Sandra, Andrejević P., Tina, Marković R., Violeta, Perdih, Franc, Turel, Iztok, Djuran I., Miloš, Nikodinović-Runić, Jasmina, Glišić Đ., Biljana, "Structural characterization and antitumor activity of platinum(II) complexes with phenothiazine and N-methylphenothiazine" in 16th International Symposium on Applied Bioinorganic Chemistry (2023):195,
https://hdl.handle.net/21.15107/rcub_imagine_1925 .

TY  - JOUR
AU  - Ašanin, Darko
AU  - Andrejević, Tina
AU  - Nenadović, Marija
AU  - Rodić, Marko
AU  - Vojnović, Sandra
AU  - Djuran, Miloš
AU  - Glišić, Biljana
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0277538723003078
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2067
AB  - In the present study, synthesis of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine (1,6-naph), {[Ag(1,6-naph)(H2O)](BF4)}n (1) and [AuCl3(1,6-naph)] (2), was reported. The methods used for the structural characterization of a new compound 1 included IR, NMR (1H and 13C) and UV-Vis spectroscopy, cyclic voltammetry and single-crystal X-ray diffraction analysis. The crystallographic results showed that compound 1 represents silver(I) coordination polymer, in which 1,6-naph ligand acts as a bidentate bridging ligand connecting two Ag(I) ions via its N1 and N6 nitrogen atoms, while the third coordination site of the metal ion is occupied by the water oxygen atom, resulted in a T-shape geometry. Compounds 1 and 2 were evaluated in vitro for antimicrobial activity against five bacterial and two Candida species, while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5). Compound 1 has manifested a remarkable antifungal activity on both tested Candida strains (C. albicans and C. parapsilosis) with minimal inhibitory concentrations (MICs) of 1.43 and 11.38 µM (0.49 and 3.9 µg/mL), respectively, while no significant antimicrobial activity was observed for 2. Moreover, silver(I) coordination polymer 1 inhibits the hyphae formation of C. albicans at subinhibitory concentration. The binding affinity of both compounds 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, indicating their ability to interact with these biomolecules, with compound 2 being more reactive.
T2  - Polyhedron
T1  - Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine
IS  - 1
SP  - 116585
VL  - 244
DO  - 10.1016/j.poly.2023.116585
ER  - 

@article{
author = "Ašanin, Darko and Andrejević, Tina and Nenadović, Marija and Rodić, Marko and Vojnović, Sandra and Djuran, Miloš and Glišić, Biljana",
year = "2023",
abstract = "In the present study, synthesis of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine (1,6-naph), {[Ag(1,6-naph)(H2O)](BF4)}n (1) and [AuCl3(1,6-naph)] (2), was reported. The methods used for the structural characterization of a new compound 1 included IR, NMR (1H and 13C) and UV-Vis spectroscopy, cyclic voltammetry and single-crystal X-ray diffraction analysis. The crystallographic results showed that compound 1 represents silver(I) coordination polymer, in which 1,6-naph ligand acts as a bidentate bridging ligand connecting two Ag(I) ions via its N1 and N6 nitrogen atoms, while the third coordination site of the metal ion is occupied by the water oxygen atom, resulted in a T-shape geometry. Compounds 1 and 2 were evaluated in vitro for antimicrobial activity against five bacterial and two Candida species, while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5). Compound 1 has manifested a remarkable antifungal activity on both tested Candida strains (C. albicans and C. parapsilosis) with minimal inhibitory concentrations (MICs) of 1.43 and 11.38 µM (0.49 and 3.9 µg/mL), respectively, while no significant antimicrobial activity was observed for 2. Moreover, silver(I) coordination polymer 1 inhibits the hyphae formation of C. albicans at subinhibitory concentration. The binding affinity of both compounds 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, indicating their ability to interact with these biomolecules, with compound 2 being more reactive.",
journal = "Polyhedron",
title = "Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine",
number = "1",
pages = "116585",
volume = "244",
doi = "10.1016/j.poly.2023.116585"
}

Ašanin, D., Andrejević, T., Nenadović, M., Rodić, M., Vojnović, S., Djuran, M.,& Glišić, B.. (2023). Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine. in Polyhedron, 244(1), 116585.
https://doi.org/10.1016/j.poly.2023.116585

Ašanin D, Andrejević T, Nenadović M, Rodić M, Vojnović S, Djuran M, Glišić B. Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine. in Polyhedron. 2023;244(1):116585.
doi:10.1016/j.poly.2023.116585 .

Ašanin, Darko, Andrejević, Tina, Nenadović, Marija, Rodić, Marko, Vojnović, Sandra, Djuran, Miloš, Glišić, Biljana, "Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine" in Polyhedron, 244, no. 1 (2023):116585,
https://doi.org/10.1016/j.poly.2023.116585 . .

TY  - CONF
AU  - Pantelić, Lena
AU  - Lazić, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Vojnović, Sandra
PY  - 2023
UR  - https://www.accelevents.com/e/circular-bioeconomy-2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1923
AB  - Potato industry is one of the major food industries that generates considerable amounts of
potato peels and wastewater (1). These wastes can then be utilized as components of
microbial media in biotechnological production of pyocyanin (PYO) and 1-hydroxyphenazine
(1-HP) using Pseudomonas aeruginosa (2). PYO and 1-HP possess important biological
activities, thus could be applied in the field of medicine, and can be used as biocontrolling
agents (2). However, their application is hindered due to high costs associated with their large
scale production.
In this work, we established a fermentation process which utilizes either potato peels or
potato wastewater as the sole nutrient source to obtain PYO and 1-HP. P. aeruginosa BK25H
strain was selected from our in-house collection. This approach afforded 10 mg/l PYO and
9 mg/l 1-HP using potato wastewater and 15 mg/l PYO and 11 g/l 1-HP using potato peels
after 24 h incubation. This work is the step towards zero-pollution and conversion of waste
to valuable microbial products.
C3  - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming
T1  - A biotechnological process for the production of pyocyanin and 1-hydroxyphenazine using waste streams from the potato chips industry
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1923
ER  - 

@conference{
author = "Pantelić, Lena and Lazić, Jelena and Nikodinović-Runić, Jasmina and Vojnović, Sandra",
year = "2023",
abstract = "Potato industry is one of the major food industries that generates considerable amounts of
potato peels and wastewater (1). These wastes can then be utilized as components of
microbial media in biotechnological production of pyocyanin (PYO) and 1-hydroxyphenazine
(1-HP) using Pseudomonas aeruginosa (2). PYO and 1-HP possess important biological
activities, thus could be applied in the field of medicine, and can be used as biocontrolling
agents (2). However, their application is hindered due to high costs associated with their large
scale production.
In this work, we established a fermentation process which utilizes either potato peels or
potato wastewater as the sole nutrient source to obtain PYO and 1-HP. P. aeruginosa BK25H
strain was selected from our in-house collection. This approach afforded 10 mg/l PYO and
9 mg/l 1-HP using potato wastewater and 15 mg/l PYO and 11 g/l 1-HP using potato peels
after 24 h incubation. This work is the step towards zero-pollution and conversion of waste
to valuable microbial products.",
journal = "Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming",
title = "A biotechnological process for the production of pyocyanin and 1-hydroxyphenazine using waste streams from the potato chips industry",
pages = "53",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1923"
}

Pantelić, L., Lazić, J., Nikodinović-Runić, J.,& Vojnović, S.. (2023). A biotechnological process for the production of pyocyanin and 1-hydroxyphenazine using waste streams from the potato chips industry. in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 53.
https://hdl.handle.net/21.15107/rcub_imagine_1923

Pantelić L, Lazić J, Nikodinović-Runić J, Vojnović S. A biotechnological process for the production of pyocyanin and 1-hydroxyphenazine using waste streams from the potato chips industry. in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming. 2023;:53.
https://hdl.handle.net/21.15107/rcub_imagine_1923 .

Pantelić, Lena, Lazić, Jelena, Nikodinović-Runić, Jasmina, Vojnović, Sandra, "A biotechnological process for the production of pyocyanin and 1-hydroxyphenazine using waste streams from the potato chips industry" in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming (2023):53,
https://hdl.handle.net/21.15107/rcub_imagine_1923 .

TY  - JOUR
AU  - Ašanin, Darko
AU  - Andrejević, Tina
AU  - Nenadović, Marija
AU  - Rodić, Marko
AU  - Vojnović, Sandra
AU  - Djuran, Miloš
AU  - Glišić, Biljana
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0277538723003078
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2076
AB  - In the present study, synthesis of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine (1,6-naph), {[Ag(1,6-naph)(H2O)](BF4)}n (1) and [AuCl3(1,6-naph)] (2), was reported. The methods used for the structural characterization of a new compound 1 included IR, NMR (1H and 13C) and UV-Vis spectroscopy, cyclic voltammetry and single-crystal X-ray diffraction analysis. The crystallographic results showed that compound 1 represents silver(I) coordination polymer, in which 1,6-naph ligand acts as a bidentate bridging ligand connecting two Ag(I) ions via its N1 and N6 nitrogen atoms, while the third coordination site of the metal ion is occupied by the water oxygen atom, resulted in a T-shape geometry. Compounds 1 and 2 were evaluated in vitro for antimicrobial activity against five bacterial and two Candida species, while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5). Compound 1 has manifested a remarkable antifungal activity on both tested Candida strains (C. albicans and C. parapsilosis) with minimal inhibitory concentrations (MICs) of 1.43 and 11.38 µM (0.49 and 3.9 µg/mL), respectively, while no significant antimicrobial activity was observed for 2. Moreover, silver(I) coordination polymer 1 inhibits the hyphae formation of C. albicans at subinhibitory concentration. The binding affinity of both compounds 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, indicating their ability to interact with these biomolecules, with compound 2 being more reactive.
T2  - Polyhedron
T1  - Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine
IS  - 1
SP  - 116585
VL  - 244
DO  - 10.1016/j.poly.2023.116585
ER  - 

@article{
author = "Ašanin, Darko and Andrejević, Tina and Nenadović, Marija and Rodić, Marko and Vojnović, Sandra and Djuran, Miloš and Glišić, Biljana",
year = "2023",
abstract = "In the present study, synthesis of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine (1,6-naph), {[Ag(1,6-naph)(H2O)](BF4)}n (1) and [AuCl3(1,6-naph)] (2), was reported. The methods used for the structural characterization of a new compound 1 included IR, NMR (1H and 13C) and UV-Vis spectroscopy, cyclic voltammetry and single-crystal X-ray diffraction analysis. The crystallographic results showed that compound 1 represents silver(I) coordination polymer, in which 1,6-naph ligand acts as a bidentate bridging ligand connecting two Ag(I) ions via its N1 and N6 nitrogen atoms, while the third coordination site of the metal ion is occupied by the water oxygen atom, resulted in a T-shape geometry. Compounds 1 and 2 were evaluated in vitro for antimicrobial activity against five bacterial and two Candida species, while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5). Compound 1 has manifested a remarkable antifungal activity on both tested Candida strains (C. albicans and C. parapsilosis) with minimal inhibitory concentrations (MICs) of 1.43 and 11.38 µM (0.49 and 3.9 µg/mL), respectively, while no significant antimicrobial activity was observed for 2. Moreover, silver(I) coordination polymer 1 inhibits the hyphae formation of C. albicans at subinhibitory concentration. The binding affinity of both compounds 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, indicating their ability to interact with these biomolecules, with compound 2 being more reactive.",
journal = "Polyhedron",
title = "Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine",
number = "1",
pages = "116585",
volume = "244",
doi = "10.1016/j.poly.2023.116585"
}

Ašanin, D., Andrejević, T., Nenadović, M., Rodić, M., Vojnović, S., Djuran, M.,& Glišić, B.. (2023). Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine. in Polyhedron, 244(1), 116585.
https://doi.org/10.1016/j.poly.2023.116585

Ašanin D, Andrejević T, Nenadović M, Rodić M, Vojnović S, Djuran M, Glišić B. Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine. in Polyhedron. 2023;244(1):116585.
doi:10.1016/j.poly.2023.116585 .

Ašanin, Darko, Andrejević, Tina, Nenadović, Marija, Rodić, Marko, Vojnović, Sandra, Djuran, Miloš, Glišić, Biljana, "Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine" in Polyhedron, 244, no. 1 (2023):116585,
https://doi.org/10.1016/j.poly.2023.116585 . .

TY  - DATA
AU  - Andrejević, Tina
AU  - Aleksić, Ivana
AU  - Kljun, Jakob
AU  - Počkaj, Marta
AU  - Zlatar, Matija
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
AU  - Turel, Iztok
AU  - Djuran, Miloš
AU  - Glišić, Biljana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1791
AB  - Dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz) was used as a ligand for the synthesis of new copper(II) and silver(I) complexes, [CuCl2(py-2pz)]2 (1), [Cu(CF3SO3)(H2O)(py-2pz)2]CF3SO3·2H2O (2), [Ag(py-2pz)2]PF6 (3) and {[Ag(NO3)(py-2pz)]·0.5H2O}n (4). The complexes were characterized by spectroscopic and electrochemical methods, while their structures were determined by single crystal X-ray diffraction analysis. The X-ray analysis revealed the bidentate coordination mode of py-2pz to the corresponding metal ion via its pyridine and pyrazine nitrogen atoms in all complexes, while in polynuclear complex 4, the heterocyclic pyrazine ring of one py-2pz additionally behaves as a bridging ligand between two Ag(I) ions. DFT calculations were performed to elucidate the structures of the investigated complexes in solution. The antimicrobial potential of the complexes 1–4 was evaluated against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two Candida (C. albicans and C. parapsilosis) species. Silver(I) complexes 3 and 4 have shown good antibacterial and antifungal properties with minimal inhibitory concentration (MIC) values ranging from 4.9 to 39.0 μM (3.9–31.2 μg mL−1). All complexes inhibited the filamentation of C. albicans and hyphae formation, while silver(I) complexes 3 and 4 had also the ability to inhibit the biofilm formation process of this fungus. The binding affinity of the complexes 1–4 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy to clarify the mode of their antimicrobial activity. Catechol oxidase biomimetic catalytic activity of copper(II) complexes 1 and 2 was additionally investigated by using 3,5-di-tert-butylcatechol (3,5-DTBC) and o-aminophenol (OAP) as substrates.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T2  - RSC Advances
T1  - Supplementary data for article:Andrejević, T. P., Aleksic, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnovic, S., Nikodinovic-Runic, J., Turel, I., Djuran, M. I., & Glišić, B. Đ. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): The influence of the metal ion on the antimicrobial potential of the complex. RSC Advances, 13(7), 4376–4393. https://doi.org/10.1039/D2RA07401J
EP  - 4393
IS  - 7
SP  - 4376
VL  - 13
DO  - 10.1039/D2RA07401J
ER  - 

@misc{
author = "Andrejević, Tina and Aleksić, Ivana and Kljun, Jakob and Počkaj, Marta and Zlatar, Matija and Vojnović, Sandra and Nikodinović-Runić, Jasmina and Turel, Iztok and Djuran, Miloš and Glišić, Biljana",
year = "2023",
abstract = "Dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz) was used as a ligand for the synthesis of new copper(II) and silver(I) complexes, [CuCl2(py-2pz)]2 (1), [Cu(CF3SO3)(H2O)(py-2pz)2]CF3SO3·2H2O (2), [Ag(py-2pz)2]PF6 (3) and {[Ag(NO3)(py-2pz)]·0.5H2O}n (4). The complexes were characterized by spectroscopic and electrochemical methods, while their structures were determined by single crystal X-ray diffraction analysis. The X-ray analysis revealed the bidentate coordination mode of py-2pz to the corresponding metal ion via its pyridine and pyrazine nitrogen atoms in all complexes, while in polynuclear complex 4, the heterocyclic pyrazine ring of one py-2pz additionally behaves as a bridging ligand between two Ag(I) ions. DFT calculations were performed to elucidate the structures of the investigated complexes in solution. The antimicrobial potential of the complexes 1–4 was evaluated against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two Candida (C. albicans and C. parapsilosis) species. Silver(I) complexes 3 and 4 have shown good antibacterial and antifungal properties with minimal inhibitory concentration (MIC) values ranging from 4.9 to 39.0 μM (3.9–31.2 μg mL−1). All complexes inhibited the filamentation of C. albicans and hyphae formation, while silver(I) complexes 3 and 4 had also the ability to inhibit the biofilm formation process of this fungus. The binding affinity of the complexes 1–4 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy to clarify the mode of their antimicrobial activity. Catechol oxidase biomimetic catalytic activity of copper(II) complexes 1 and 2 was additionally investigated by using 3,5-di-tert-butylcatechol (3,5-DTBC) and o-aminophenol (OAP) as substrates.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances, RSC Advances",
title = "Supplementary data for article:Andrejević, T. P., Aleksic, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnovic, S., Nikodinovic-Runic, J., Turel, I., Djuran, M. I., & Glišić, B. Đ. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): The influence of the metal ion on the antimicrobial potential of the complex. RSC Advances, 13(7), 4376–4393. https://doi.org/10.1039/D2RA07401J",
pages = "4393-4376",
number = "7",
volume = "13",
doi = "10.1039/D2RA07401J"
}

Andrejević, T., Aleksić, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnović, S., Nikodinović-Runić, J., Turel, I., Djuran, M.,& Glišić, B.. (2023). Supplementary data for article:Andrejević, T. P., Aleksic, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnovic, S., Nikodinovic-Runic, J., Turel, I., Djuran, M. I., & Glišić, B. Đ. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): The influence of the metal ion on the antimicrobial potential of the complex. RSC Advances, 13(7), 4376–4393. https://doi.org/10.1039/D2RA07401J. in RSC Advances
Royal Society of Chemistry., 13(7), 4376-4393.
https://doi.org/10.1039/D2RA07401J

Andrejević T, Aleksić I, Kljun J, Počkaj M, Zlatar M, Vojnović S, Nikodinović-Runić J, Turel I, Djuran M, Glišić B. Supplementary data for article:Andrejević, T. P., Aleksic, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnovic, S., Nikodinovic-Runic, J., Turel, I., Djuran, M. I., & Glišić, B. Đ. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): The influence of the metal ion on the antimicrobial potential of the complex. RSC Advances, 13(7), 4376–4393. https://doi.org/10.1039/D2RA07401J. in RSC Advances. 2023;13(7):4376-4393.
doi:10.1039/D2RA07401J .

Andrejević, Tina, Aleksić, Ivana, Kljun, Jakob, Počkaj, Marta, Zlatar, Matija, Vojnović, Sandra, Nikodinović-Runić, Jasmina, Turel, Iztok, Djuran, Miloš, Glišić, Biljana, "Supplementary data for article:Andrejević, T. P., Aleksic, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnovic, S., Nikodinovic-Runic, J., Turel, I., Djuran, M. I., & Glišić, B. Đ. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): The influence of the metal ion on the antimicrobial potential of the complex. RSC Advances, 13(7), 4376–4393. https://doi.org/10.1039/D2RA07401J" in RSC Advances, 13, no. 7 (2023):4376-4393,
https://doi.org/10.1039/D2RA07401J . .

TY  - JOUR
AU  - Başpınar, Yasemin
AU  - Gürbüz, Perihan
AU  - Dilem Doğan, Şengül
AU  - Gözde Gündüz, Miyase
AU  - Aleksić, Ivana
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
AU  - Yavuz Paksoy, Mehmet
PY  - 2023
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/cbdv.202300134
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1798
AB  - This is the first report on the separation and biological assessment of all metabolites derived from Pulicaria armena (Asteraceae) which is an endemic species narrowly distributed in the eastern part of Turkey. The phytochemical analysis of P. armena resulted in the identification of one simple phenolic glucoside together with eight flavon and flavonol derivatives whose chemical structures were elucidated by NMR experiments and by the comparison of the spectral data with the relevant literature. The screening of all molecules for their antimicrobial, anti-quorum sensing, and cytotoxic activities revealed the biological potential of some of the isolated compounds. Additionally, quorum sensing inhibitory activity of quercetagetin 5,7,3’ trimethyl ether was supported by molecular docking studies in the active site of LasR which is the primary regulator of this cell-to-cell communication system in bacteria. Lastly, the critical molecular properties indicating drug-likeness of the compounds isolated from P. armena were predicted. As microbial infections can be a serious problem for cancer patients with compromised immune systems, this comprehensive phytochemical research on P. armena with its anti-quorum sensing and cytotoxic compounds can provide a new approach to the treatment.
T2  - Chemistry & Biodiversity
T1  - Investigation of Antimicrobial, Anti-Quorum Sensing, and Cytotoxic Activities of Flavonoids Isolated from Pulicaria armena Boiss. & Kotschy ex Boiss. (Asteraceae)
SP  - e202300134
VL  - 20
DO  - 10.1002/cbdv.202300134
ER  - 

@article{
author = "Başpınar, Yasemin and Gürbüz, Perihan and Dilem Doğan, Şengül and Gözde Gündüz, Miyase and Aleksić, Ivana and Vojnović, Sandra and Nikodinović-Runić, Jasmina and Yavuz Paksoy, Mehmet",
year = "2023",
abstract = "This is the first report on the separation and biological assessment of all metabolites derived from Pulicaria armena (Asteraceae) which is an endemic species narrowly distributed in the eastern part of Turkey. The phytochemical analysis of P. armena resulted in the identification of one simple phenolic glucoside together with eight flavon and flavonol derivatives whose chemical structures were elucidated by NMR experiments and by the comparison of the spectral data with the relevant literature. The screening of all molecules for their antimicrobial, anti-quorum sensing, and cytotoxic activities revealed the biological potential of some of the isolated compounds. Additionally, quorum sensing inhibitory activity of quercetagetin 5,7,3’ trimethyl ether was supported by molecular docking studies in the active site of LasR which is the primary regulator of this cell-to-cell communication system in bacteria. Lastly, the critical molecular properties indicating drug-likeness of the compounds isolated from P. armena were predicted. As microbial infections can be a serious problem for cancer patients with compromised immune systems, this comprehensive phytochemical research on P. armena with its anti-quorum sensing and cytotoxic compounds can provide a new approach to the treatment.",
journal = "Chemistry & Biodiversity",
title = "Investigation of Antimicrobial, Anti-Quorum Sensing, and Cytotoxic Activities of Flavonoids Isolated from Pulicaria armena Boiss. & Kotschy ex Boiss. (Asteraceae)",
pages = "e202300134",
volume = "20",
doi = "10.1002/cbdv.202300134"
}

Başpınar, Y., Gürbüz, P., Dilem Doğan, Ş., Gözde Gündüz, M., Aleksić, I., Vojnović, S., Nikodinović-Runić, J.,& Yavuz Paksoy, M.. (2023). Investigation of Antimicrobial, Anti-Quorum Sensing, and Cytotoxic Activities of Flavonoids Isolated from Pulicaria armena Boiss. & Kotschy ex Boiss. (Asteraceae). in Chemistry & Biodiversity, 20, e202300134.
https://doi.org/10.1002/cbdv.202300134

Başpınar Y, Gürbüz P, Dilem Doğan Ş, Gözde Gündüz M, Aleksić I, Vojnović S, Nikodinović-Runić J, Yavuz Paksoy M. Investigation of Antimicrobial, Anti-Quorum Sensing, and Cytotoxic Activities of Flavonoids Isolated from Pulicaria armena Boiss. & Kotschy ex Boiss. (Asteraceae). in Chemistry & Biodiversity. 2023;20:e202300134.
doi:10.1002/cbdv.202300134 .

Başpınar, Yasemin, Gürbüz, Perihan, Dilem Doğan, Şengül, Gözde Gündüz, Miyase, Aleksić, Ivana, Vojnović, Sandra, Nikodinović-Runić, Jasmina, Yavuz Paksoy, Mehmet, "Investigation of Antimicrobial, Anti-Quorum Sensing, and Cytotoxic Activities of Flavonoids Isolated from Pulicaria armena Boiss. & Kotschy ex Boiss. (Asteraceae)" in Chemistry & Biodiversity, 20 (2023):e202300134,
https://doi.org/10.1002/cbdv.202300134 . .

TY  - JOUR
AU  - Pantelić, Lena
AU  - Bogojević Škaro, Sanja
AU  - Vojnović, Sandra
AU  - Oliveira, Rui
AU  - Lazić, Jelena
AU  - Ilić-Tomić, Tatjana
AU  - Milivojević, Dušan
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0141022923001308
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2078
AB  - Phenazines, including pyocyanin (PYO) and 1-hydroxyphenazine (1-HP) are extracellular secondary metabolites and multifunctional pigments of Pseudomonas aeruginosa responsible for its blue-green color. These versatile molecules are electrochemically active, involved in significant biological activities giving fitness to the host, but also recognized as antimicrobial and anticancer agents. Their wider application is still limited partly due to the cost of carbon substrate for production, which can be solved by the utilization of carbon from food waste within the biorefinery concept. In this study, a variety of food waste streams (banana peel, potato peel, potato washing, stale bread, yoghurt, processed meat, boiled eggs and mixed canteen waste) was used as sole nutrient source in submerged cultures of P. aeruginosa BK25H. Stale bread was identified as the most suitable substrate to support phenazine biopigments production and bacterial growth. This was further increased in 5-liter fermenter when on average 5.2mgL-1 of PYO and 4.4mgL-1 of 1-HP were purified after 24h batch cultivations from the fermentation medium consisting of homogenized stale bread in tap water. Purified biopigments showed moderate antimicrobial activity, and showed different toxicity profiles, with PYO not being toxic against Caenorhabditis elegans, a free-living soil nematode up to 300µgmL-1 and 1-HP showing lethal effects at 75µgmL-1. Therefore, stale bread waste stream with minimal pretreatment should be considered as suitable biorefinery feedstock, as it can support the production of valuable biopigments such as phenazines.
T2  - Enzyme and Microbial Technology
T1  - Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine
SP  - 110322
VL  - 171
DO  - 10.1016/j.enzmictec.2023.110322
ER  - 

@article{
author = "Pantelić, Lena and Bogojević Škaro, Sanja and Vojnović, Sandra and Oliveira, Rui and Lazić, Jelena and Ilić-Tomić, Tatjana and Milivojević, Dušan and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Phenazines, including pyocyanin (PYO) and 1-hydroxyphenazine (1-HP) are extracellular secondary metabolites and multifunctional pigments of Pseudomonas aeruginosa responsible for its blue-green color. These versatile molecules are electrochemically active, involved in significant biological activities giving fitness to the host, but also recognized as antimicrobial and anticancer agents. Their wider application is still limited partly due to the cost of carbon substrate for production, which can be solved by the utilization of carbon from food waste within the biorefinery concept. In this study, a variety of food waste streams (banana peel, potato peel, potato washing, stale bread, yoghurt, processed meat, boiled eggs and mixed canteen waste) was used as sole nutrient source in submerged cultures of P. aeruginosa BK25H. Stale bread was identified as the most suitable substrate to support phenazine biopigments production and bacterial growth. This was further increased in 5-liter fermenter when on average 5.2mgL-1 of PYO and 4.4mgL-1 of 1-HP were purified after 24h batch cultivations from the fermentation medium consisting of homogenized stale bread in tap water. Purified biopigments showed moderate antimicrobial activity, and showed different toxicity profiles, with PYO not being toxic against Caenorhabditis elegans, a free-living soil nematode up to 300µgmL-1 and 1-HP showing lethal effects at 75µgmL-1. Therefore, stale bread waste stream with minimal pretreatment should be considered as suitable biorefinery feedstock, as it can support the production of valuable biopigments such as phenazines.",
journal = "Enzyme and Microbial Technology",
title = "Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine",
pages = "110322",
volume = "171",
doi = "10.1016/j.enzmictec.2023.110322"
}

Pantelić, L., Bogojević Škaro, S., Vojnović, S., Oliveira, R., Lazić, J., Ilić-Tomić, T., Milivojević, D.,& Nikodinović-Runić, J.. (2023). Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine. in Enzyme and Microbial Technology, 171, 110322.
https://doi.org/10.1016/j.enzmictec.2023.110322

Pantelić L, Bogojević Škaro S, Vojnović S, Oliveira R, Lazić J, Ilić-Tomić T, Milivojević D, Nikodinović-Runić J. Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine. in Enzyme and Microbial Technology. 2023;171:110322.
doi:10.1016/j.enzmictec.2023.110322 .

Pantelić, Lena, Bogojević Škaro, Sanja, Vojnović, Sandra, Oliveira, Rui, Lazić, Jelena, Ilić-Tomić, Tatjana, Milivojević, Dušan, Nikodinović-Runić, Jasmina, "Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine" in Enzyme and Microbial Technology, 171 (2023):110322,
https://doi.org/10.1016/j.enzmictec.2023.110322 . .

TY  - JOUR
AU  - Milzarek, Tobias M.
AU  - Stevanović, Milena
AU  - Milivojević, Dušan
AU  - Vojnović, Sandra
AU  - Iliasov, Denis
AU  - Wolf, Diana
AU  - Mascher, Thorsten
AU  - Nikodinović-Runić, Jasmina
AU  - Gulder, Tobias A. M.
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2077
AB  - The ambigols are cyanobacterial natural products characterized by three polychlorinated aromatic building blocks connected by biaryl and biaryl ether bridges. All ambigols known to date possess promising biological activities. Most significantly, ambigol A was reported to have antibacterial activity against Gram-positive bacteria, such as Bacillus megaterium and B. subtilis. We established a diverse compound library for in-depth biological evaluation building on our previous bio- and total synthetic research on this natural product family. To explore the antimicrobial potential in detail and to determine initial structure–activity relationships of this product class, a large set of dimeric and trimeric compounds were screened against selected bacterial and Candida target strains. Our results reveal exceptional antibiotic activity of the ambigols, especially against challenging clinical isolates.
T2  - ACS Infectious Diseases
T2  - ACS Infectious DiseasesACS Infect. Dis.
T1  - Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs
DO  - 10.1021/acsinfecdis.3c00232
ER  - 

@article{
author = "Milzarek, Tobias M. and Stevanović, Milena and Milivojević, Dušan and Vojnović, Sandra and Iliasov, Denis and Wolf, Diana and Mascher, Thorsten and Nikodinović-Runić, Jasmina and Gulder, Tobias A. M.",
year = "2023",
abstract = "The ambigols are cyanobacterial natural products characterized by three polychlorinated aromatic building blocks connected by biaryl and biaryl ether bridges. All ambigols known to date possess promising biological activities. Most significantly, ambigol A was reported to have antibacterial activity against Gram-positive bacteria, such as Bacillus megaterium and B. subtilis. We established a diverse compound library for in-depth biological evaluation building on our previous bio- and total synthetic research on this natural product family. To explore the antimicrobial potential in detail and to determine initial structure–activity relationships of this product class, a large set of dimeric and trimeric compounds were screened against selected bacterial and Candida target strains. Our results reveal exceptional antibiotic activity of the ambigols, especially against challenging clinical isolates.",
journal = "ACS Infectious Diseases, ACS Infectious DiseasesACS Infect. Dis.",
title = "Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs",
doi = "10.1021/acsinfecdis.3c00232"
}

Milzarek, T. M., Stevanović, M., Milivojević, D., Vojnović, S., Iliasov, D., Wolf, D., Mascher, T., Nikodinović-Runić, J.,& Gulder, T. A. M.. (2023). Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs. in ACS Infectious Diseases.
https://doi.org/10.1021/acsinfecdis.3c00232

Milzarek TM, Stevanović M, Milivojević D, Vojnović S, Iliasov D, Wolf D, Mascher T, Nikodinović-Runić J, Gulder TAM. Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs. in ACS Infectious Diseases. 2023;.
doi:10.1021/acsinfecdis.3c00232 .

Milzarek, Tobias M., Stevanović, Milena, Milivojević, Dušan, Vojnović, Sandra, Iliasov, Denis, Wolf, Diana, Mascher, Thorsten, Nikodinović-Runić, Jasmina, Gulder, Tobias A. M., "Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs" in ACS Infectious Diseases (2023),
https://doi.org/10.1021/acsinfecdis.3c00232 . .

TY  - CONF
AU  - Stevanović, Milena
AU  - Janković, Vukašin
AU  - Filipović, Vuk
AU  - Ilić-Tomić, Tatjana
AU  - Vojnović, Sandra
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1915
AB  - The genus Streptomyces has been studied for the vast secondary metabolite
production, biological activity of crude extracts, as well as colouring properties on textile
materials [1, 2]. Streptomyces sp. BV365 is a potent producer of yellow pigments, used to
efficiently colour different natural textiles [3]. Utilization of food waste for bacterial growth
and production of pigments and antifungals [4] could contribute to conversion of waste to
valuable molecules, providing more economically acceptable and more eco-friendly
generated biotherapeutics, enzymes and pigments.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365
EP  - 168
SP  - 167
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1915
ER  - 

@conference{
author = "Stevanović, Milena and Janković, Vukašin and Filipović, Vuk and Ilić-Tomić, Tatjana and Vojnović, Sandra",
year = "2023",
abstract = "The genus Streptomyces has been studied for the vast secondary metabolite
production, biological activity of crude extracts, as well as colouring properties on textile
materials [1, 2]. Streptomyces sp. BV365 is a potent producer of yellow pigments, used to
efficiently colour different natural textiles [3]. Utilization of food waste for bacterial growth
and production of pigments and antifungals [4] could contribute to conversion of waste to
valuable molecules, providing more economically acceptable and more eco-friendly
generated biotherapeutics, enzymes and pigments.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365",
pages = "168-167",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1915"
}

Stevanović, M., Janković, V., Filipović, V., Ilić-Tomić, T.,& Vojnović, S.. (2023). Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 9, 167-168.
https://hdl.handle.net/21.15107/rcub_imagine_1915

Stevanović M, Janković V, Filipović V, Ilić-Tomić T, Vojnović S. Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:167-168.
https://hdl.handle.net/21.15107/rcub_imagine_1915 .

Stevanović, Milena, Janković, Vukašin, Filipović, Vuk, Ilić-Tomić, Tatjana, Vojnović, Sandra, "Food waste as a nutrient source for the production of biopigment in Streptomyces sp. BV365" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):167-168,
https://hdl.handle.net/21.15107/rcub_imagine_1915 .

TY  - JOUR
AU  - Vuković, Jovana S.
AU  - Radić, Marija M. Babić
AU  - Trifunovi, Saša B.
AU  - Koch, Thomas
AU  - Perić-Grujić, Aleksandra A.
AU  - Vojnović, Sandra
AU  - Tomić, Simonida Lj.
PY  - 2023
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/macp.202300310
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2165
AB  - Antibacterial hydrogels, as an advanced approach, can create optimal conditions for wound healing, even in the fight against stubborn and difficult-to-treat wound infections. Interestingly, pH is an often neglected clinical parameter, although it has a significant impact on the wound healing process. At different stages of wound healing, the pH in the wound bed changes from slightly alkaline to neutral to acidic. To develop novel pH-sensitive antibacterial hydrogel dressings, Zn2+-loaded poly(2-hydroxyethyl acrylate/itaconic acid) hydrogels were synthesized. The hydrogels exhibit pH-sensitive swelling in the physiologically relevant pH range, with a pronounced swelling ability at neutral pH. The controlled release of Zn2+ occurs in a buffer of pH 7.40 at 37°C. The liquid transport mechanism and release kinetics are evaluated using the specific kinetic models of Ritger-Peppas and Peppas-Sahlin. The effect of Zn2+ on structural, thermal, swelling, cytocompatibility, and antibacterial properties is evaluated by Fourier transform infrared spectroscopy, differential scanning calorimetry, swelling studies, MTT, and antibacterial tests. The hydrogels show excellent antibacterial activity against Escherichia coli. The research opens new perspectives for efficient wound healing management, and the extension of the study will be orchestrated by optimising the hydrogel composition to achieve improved performance. This article is protected by copyright. All rights reserved
T2  - Macromolecular Chemistry and Physics
T2  - Macromolecular Chemistry and Physics
T1  - Zn2+/Poly(2-Hydroxyethyl Acrylate/Itaconic Acid) Hydrogels as Potential Antibacterial Wound Dressings
IS  - n/a
SP  - 2300310
VL  - n/a
DO  - 10.1002/macp.202300310
ER  - 

@article{
author = "Vuković, Jovana S. and Radić, Marija M. Babić and Trifunovi, Saša B. and Koch, Thomas and Perić-Grujić, Aleksandra A. and Vojnović, Sandra and Tomić, Simonida Lj.",
year = "2023",
abstract = "Antibacterial hydrogels, as an advanced approach, can create optimal conditions for wound healing, even in the fight against stubborn and difficult-to-treat wound infections. Interestingly, pH is an often neglected clinical parameter, although it has a significant impact on the wound healing process. At different stages of wound healing, the pH in the wound bed changes from slightly alkaline to neutral to acidic. To develop novel pH-sensitive antibacterial hydrogel dressings, Zn2+-loaded poly(2-hydroxyethyl acrylate/itaconic acid) hydrogels were synthesized. The hydrogels exhibit pH-sensitive swelling in the physiologically relevant pH range, with a pronounced swelling ability at neutral pH. The controlled release of Zn2+ occurs in a buffer of pH 7.40 at 37°C. The liquid transport mechanism and release kinetics are evaluated using the specific kinetic models of Ritger-Peppas and Peppas-Sahlin. The effect of Zn2+ on structural, thermal, swelling, cytocompatibility, and antibacterial properties is evaluated by Fourier transform infrared spectroscopy, differential scanning calorimetry, swelling studies, MTT, and antibacterial tests. The hydrogels show excellent antibacterial activity against Escherichia coli. The research opens new perspectives for efficient wound healing management, and the extension of the study will be orchestrated by optimising the hydrogel composition to achieve improved performance. This article is protected by copyright. All rights reserved",
journal = "Macromolecular Chemistry and Physics, Macromolecular Chemistry and Physics",
title = "Zn2+/Poly(2-Hydroxyethyl Acrylate/Itaconic Acid) Hydrogels as Potential Antibacterial Wound Dressings",
number = "n/a",
pages = "2300310",
volume = "n/a",
doi = "10.1002/macp.202300310"
}

Vuković, J. S., Radić, M. M. B., Trifunovi, S. B., Koch, T., Perić-Grujić, A. A., Vojnović, S.,& Tomić, S. Lj.. (2023). Zn2+/Poly(2-Hydroxyethyl Acrylate/Itaconic Acid) Hydrogels as Potential Antibacterial Wound Dressings. in Macromolecular Chemistry and Physics, n/a(n/a), 2300310.
https://doi.org/10.1002/macp.202300310

Vuković JS, Radić MMB, Trifunovi SB, Koch T, Perić-Grujić AA, Vojnović S, Tomić SL. Zn2+/Poly(2-Hydroxyethyl Acrylate/Itaconic Acid) Hydrogels as Potential Antibacterial Wound Dressings. in Macromolecular Chemistry and Physics. 2023;n/a(n/a):2300310.
doi:10.1002/macp.202300310 .

Vuković, Jovana S., Radić, Marija M. Babić, Trifunovi, Saša B., Koch, Thomas, Perić-Grujić, Aleksandra A., Vojnović, Sandra, Tomić, Simonida Lj., "Zn2+/Poly(2-Hydroxyethyl Acrylate/Itaconic Acid) Hydrogels as Potential Antibacterial Wound Dressings" in Macromolecular Chemistry and Physics, n/a, no. n/a (2023):2300310,
https://doi.org/10.1002/macp.202300310 . .

TY  - JOUR
AU  - Garcia, Eduardo Lanzagorta
AU  - Mojićević, Marija
AU  - Milivojević, Dušan
AU  - Aleksic, Ivana
AU  - Vojnović, Sandra
AU  - Stevanović, Milena
AU  - Murray, James
AU  - Attallah, Olivia Adly
AU  - Devine, Declan
AU  - Fournet, Margaret Brennan
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1658
AB  - Curcumin and triangular silver nanoplates (TSNP)-incorporated bacterial cellulose (BC) films present an ideal antimicrobial material for biomedical applications as they afford a complete set of requirements, including a broad range of long-lasting potency and superior efficacy antimicrobial activity, combined with low toxicity. Here, BC was produced by Komagataeibacter medellinensis ID13488 strain in the presence of curcumin in the production medium (2 and 10%). TSNP were incorporated in the produced BC/curcumin films using ex situ method (21.34 ppm) and the antimicrobial activity was evaluated against Escherichia coli ATCC95922 and Staphylococcus aureus ATCC25923 bacterial strains. Biological activity of these natural products was assessed in cytotoxicity assay against lung fibroblasts and in vivo using Caenorhabditis elegans and Danio rerio as model organisms. Derived films have shown excellent antimicrobial performance with growth inhibition up to 67% for E. coli and 95% for S. aureus. In a highly positive synergistic interaction, BC films with 10% curcumin and incorporated TSNP have shown reduced toxicity with 80% MRC5 cells survival rate. It was shown that only 100% concentrations of film extracts induce low toxicity effect on model organisms’ development. The combined and synergistic advanced anti-infective functionalities of the curcumin and TSNP incorporated in BC have a high potential for development for application within the clinical setting.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates
IS  - 20
SP  - 12198
VL  - 23
DO  - 10.3390/ijms232012198
ER  - 

@article{
author = "Garcia, Eduardo Lanzagorta and Mojićević, Marija and Milivojević, Dušan and Aleksic, Ivana and Vojnović, Sandra and Stevanović, Milena and Murray, James and Attallah, Olivia Adly and Devine, Declan and Fournet, Margaret Brennan",
year = "2022",
abstract = "Curcumin and triangular silver nanoplates (TSNP)-incorporated bacterial cellulose (BC) films present an ideal antimicrobial material for biomedical applications as they afford a complete set of requirements, including a broad range of long-lasting potency and superior efficacy antimicrobial activity, combined with low toxicity. Here, BC was produced by Komagataeibacter medellinensis ID13488 strain in the presence of curcumin in the production medium (2 and 10%). TSNP were incorporated in the produced BC/curcumin films using ex situ method (21.34 ppm) and the antimicrobial activity was evaluated against Escherichia coli ATCC95922 and Staphylococcus aureus ATCC25923 bacterial strains. Biological activity of these natural products was assessed in cytotoxicity assay against lung fibroblasts and in vivo using Caenorhabditis elegans and Danio rerio as model organisms. Derived films have shown excellent antimicrobial performance with growth inhibition up to 67% for E. coli and 95% for S. aureus. In a highly positive synergistic interaction, BC films with 10% curcumin and incorporated TSNP have shown reduced toxicity with 80% MRC5 cells survival rate. It was shown that only 100% concentrations of film extracts induce low toxicity effect on model organisms’ development. The combined and synergistic advanced anti-infective functionalities of the curcumin and TSNP incorporated in BC have a high potential for development for application within the clinical setting.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates",
number = "20",
pages = "12198",
volume = "23",
doi = "10.3390/ijms232012198"
}

Garcia, E. L., Mojićević, M., Milivojević, D., Aleksic, I., Vojnović, S., Stevanović, M., Murray, J., Attallah, O. A., Devine, D.,& Fournet, M. B.. (2022). Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates. in International Journal of Molecular Sciences, 23(20), 12198.
https://doi.org/10.3390/ijms232012198

Garcia EL, Mojićević M, Milivojević D, Aleksic I, Vojnović S, Stevanović M, Murray J, Attallah OA, Devine D, Fournet MB. Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates. in International Journal of Molecular Sciences. 2022;23(20):12198.
doi:10.3390/ijms232012198 .

Garcia, Eduardo Lanzagorta, Mojićević, Marija, Milivojević, Dušan, Aleksic, Ivana, Vojnović, Sandra, Stevanović, Milena, Murray, James, Attallah, Olivia Adly, Devine, Declan, Fournet, Margaret Brennan, "Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates" in International Journal of Molecular Sciences, 23, no. 20 (2022):12198,
https://doi.org/10.3390/ijms232012198 . .

TY  - CONF
AU  - Vojnović, Sandra
AU  - Stevanović, Milena
AU  - Lazić, Jelena
AU  - Pantelić, Lena
AU  - Milojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Nikodinović-Runić, Jasmina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1751
AB  - Iako su bioterapeutici doveli do revolucionarnih promena u lečenju raka,
dokazali svoju efikasnost u saniranju mikrobnih infekcija i lečenju ljudi sa
retkim bolestima, zbog visoke cene nisu svima dostupni. Inovativni pristup gde
otpad ulazi u sastav hranjivih podloga za gajenje bakterija bi mogao dovesti do
smanjenja cene bioterapeutika. Fermentacijom na skali od 5 L je dobijeno
nekoliko bioaktivnih prirodnih proizvoda, pri čemu je različit otpad bio izvor
hranjivih materija za gajenje mikroorganizama proizvođača. Prodigiozin,
sekundarni metabolit bakterije Serratia marcescens, dobijen je gajenjem S.
marcescens u tečnoj podlozi sa homogenizovanim mesnim nareskom, a prinos
prodigiozina je poboljšan čak 10 puta u poređenju sa standardnom podlogom.1
Kada je Pseudomonas sp. BK25H gajen u podlozi sa komponentama kuhinjskog otpada
dobijen je odličan prinos piocijanina od 12,5 mg L-1. Slično, aktinomicin D i
staurosporin, sekundarni metaboliti Streptomyces sp. BV365 i 410, su dobijeni
gajenjem proizvođača u hranjivim podlogama sa komponentama poljoprivrednog
otpada ili otpada dobijenog preradom lignoceluloze. S obzirom da neka od
navedenih jedinjenja dostižu komercijalnu vrednost od 2.500,00 evra za 1 mg
(https://www.sigmaaldrich.com/RS/en/product/sigma/s5921), dobijeni rezultati idu u
prilog ideji da bi se otpadne materije mogle koristiti kao jeftine sirovine za
proizvodnju vrednih hemikalija, uz istovremeno smanjenje njihove cene, pri čemu
bi se i količina otpadnih tokova redukovala.
AB  - Иако су биотерапеутици довели до револуционарних промена у лечењу рака,
доказали своју ефикасност у санирању микробних инфекција и лечењу људи са
ретким болестима, због високе цене нису свима доступни. Иновативни приступ где
отпад улази у састав храњивих подлога за гајење бактерија би могао довести до
смањења цене биотерапеутика. Ферментацијом на скали од 5 L је добијено
неколико биоактивних природних производа, при чему је различит отпад био извор
храњивих материја за гајење микроорганизама произвођача. Продигиозин,
секундарни метаболит бактерије Serratia marcescens, добијен је гајењем S.
marcescens у течној подлози са хомогенизованим месним нареском, а принос
продигиозина је побољшан чак 10 пута у поређењу са стандардном подлогом.1
Када је Pseudomonas sp. BK25H гајен у подлози са компонентама кухињског отпада
добијен је одличан принос пиоцијанина од 12,5 mg L-1. Слично, актиномицин Д и
стауроспорин, секундарни метаболити Streptomyces sp. BV365 и 410, су добијени
гајењем произвођача у храњивим подлогама са компонентама пољопривредног
отпада или отпада добијеног прерадом лигноцелулозе. С обзиром да нека од
наведених једињења достижу комерцијалну вредност од 2.500,00 евра за 1 mg
(https://www.sigmaaldrich.com/RS/en/product/sigma/s5921), добијени резултати иду у
прилог идеји да би се отпадне материје могле користити као јефтине сировине за
производњу вредних хемикалија, уз истовремено смањење њихове цене, при чему
би се и количина отпадних токова редуковала.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije
T1  - Od otpada do bioterapeutika
T1  - Од отпада до биотерапеутика
SP  - 253
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1751
ER  - 

@conference{
author = "Vojnović, Sandra and Stevanović, Milena and Lazić, Jelena and Pantelić, Lena and Milojević, Dušan and Ilić-Tomić, Tatjana and Nikodinović-Runić, Jasmina",
year = "2022",
abstract = "Iako su bioterapeutici doveli do revolucionarnih promena u lečenju raka,
dokazali svoju efikasnost u saniranju mikrobnih infekcija i lečenju ljudi sa
retkim bolestima, zbog visoke cene nisu svima dostupni. Inovativni pristup gde
otpad ulazi u sastav hranjivih podloga za gajenje bakterija bi mogao dovesti do
smanjenja cene bioterapeutika. Fermentacijom na skali od 5 L je dobijeno
nekoliko bioaktivnih prirodnih proizvoda, pri čemu je različit otpad bio izvor
hranjivih materija za gajenje mikroorganizama proizvođača. Prodigiozin,
sekundarni metabolit bakterije Serratia marcescens, dobijen je gajenjem S.
marcescens u tečnoj podlozi sa homogenizovanim mesnim nareskom, a prinos
prodigiozina je poboljšan čak 10 puta u poređenju sa standardnom podlogom.1
Kada je Pseudomonas sp. BK25H gajen u podlozi sa komponentama kuhinjskog otpada
dobijen je odličan prinos piocijanina od 12,5 mg L-1. Slično, aktinomicin D i
staurosporin, sekundarni metaboliti Streptomyces sp. BV365 i 410, su dobijeni
gajenjem proizvođača u hranjivim podlogama sa komponentama poljoprivrednog
otpada ili otpada dobijenog preradom lignoceluloze. S obzirom da neka od
navedenih jedinjenja dostižu komercijalnu vrednost od 2.500,00 evra za 1 mg
(https://www.sigmaaldrich.com/RS/en/product/sigma/s5921), dobijeni rezultati idu u
prilog ideji da bi se otpadne materije mogle koristiti kao jeftine sirovine za
proizvodnju vrednih hemikalija, uz istovremeno smanjenje njihove cene, pri čemu
bi se i količina otpadnih tokova redukovala., Иако су биотерапеутици довели до револуционарних промена у лечењу рака,
доказали своју ефикасност у санирању микробних инфекција и лечењу људи са
ретким болестима, због високе цене нису свима доступни. Иновативни приступ где
отпад улази у састав храњивих подлога за гајење бактерија би могао довести до
смањења цене биотерапеутика. Ферментацијом на скали од 5 L је добијено
неколико биоактивних природних производа, при чему је различит отпад био извор
храњивих материја за гајење микроорганизама произвођача. Продигиозин,
секундарни метаболит бактерије Serratia marcescens, добијен је гајењем S.
marcescens у течној подлози са хомогенизованим месним нареском, а принос
продигиозина је побољшан чак 10 пута у поређењу са стандардном подлогом.1
Када је Pseudomonas sp. BK25H гајен у подлози са компонентама кухињског отпада
добијен је одличан принос пиоцијанина од 12,5 mg L-1. Слично, актиномицин Д и
стауроспорин, секундарни метаболити Streptomyces sp. BV365 и 410, су добијени
гајењем произвођача у храњивим подлогама са компонентама пољопривредног
отпада или отпада добијеног прерадом лигноцелулозе. С обзиром да нека од
наведених једињења достижу комерцијалну вредност од 2.500,00 евра за 1 mg
(https://www.sigmaaldrich.com/RS/en/product/sigma/s5921), добијени резултати иду у
прилог идеји да би се отпадне материје могле користити као јефтине сировине за
производњу вредних хемикалија, уз истовремено смањење њихове цене, при чему
би се и количина отпадних токова редуковала.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije",
title = "Od otpada do bioterapeutika, Од отпада до биотерапеутика",
pages = "253",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1751"
}

Vojnović, S., Stevanović, M., Lazić, J., Pantelić, L., Milojević, D., Ilić-Tomić, T.,& Nikodinović-Runić, J.. (2022). Od otpada do bioterapeutika. in Treći kongres biologa Srbije
Beograd : Srpsko biološko društvo., 253.
https://hdl.handle.net/21.15107/rcub_imagine_1751

Vojnović S, Stevanović M, Lazić J, Pantelić L, Milojević D, Ilić-Tomić T, Nikodinović-Runić J. Od otpada do bioterapeutika. in Treći kongres biologa Srbije. 2022;:253.
https://hdl.handle.net/21.15107/rcub_imagine_1751 .

Vojnović, Sandra, Stevanović, Milena, Lazić, Jelena, Pantelić, Lena, Milojević, Dušan, Ilić-Tomić, Tatjana, Nikodinović-Runić, Jasmina, "Od otpada do bioterapeutika" in Treći kongres biologa Srbije (2022):253,
https://hdl.handle.net/21.15107/rcub_imagine_1751 .

TY  - JOUR
AU  - Hertrampf, Gesa
AU  - Kusserow, Kalina
AU  - Vojnović, Sandra
AU  - Pavić, Aleksandar
AU  - Mueller, Jonas, I
AU  - Nikodinović-Runić, Jasmina
AU  - Gulder, Tobias A. M.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1553
AB  - The increasing emergence of resistances against established antibiotics is a substantial threat to human health. The discovery of new compounds with potent antibiotic activity is thus of utmost importance. Within this work, we identify strong antibiotic activity of the natural product myxocoumarin B from Stigmatella aurantiaca MYX-030 against a range of clinically relevant bacterial pathogens, including clinical isolates of MRSA. A focused library of structural analogs was synthesized to explore initial structure-activity relationships and to identify equipotent myxocoumarin derivatives devoid of the natural nitro substituent to significantly streamline synthetic access. The cytotoxicity of the myxocoumarins as well as their potential to cure bacterial infections in vivo was established using a zebrafish model system. Our results reveal the exceptional antibiotic activity of the myxocoumarin scaffold and hence its potential for the development of novel antibiotics.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry-A European Journal
T1  - Strong Antibiotic Activity of the Myxocoumarin Scaffold in vitro and in vivo
IS  - 32
VL  - 28
DO  - 10.1002/chem.202200394
ER  - 

@article{
author = "Hertrampf, Gesa and Kusserow, Kalina and Vojnović, Sandra and Pavić, Aleksandar and Mueller, Jonas, I and Nikodinović-Runić, Jasmina and Gulder, Tobias A. M.",
year = "2022",
abstract = "The increasing emergence of resistances against established antibiotics is a substantial threat to human health. The discovery of new compounds with potent antibiotic activity is thus of utmost importance. Within this work, we identify strong antibiotic activity of the natural product myxocoumarin B from Stigmatella aurantiaca MYX-030 against a range of clinically relevant bacterial pathogens, including clinical isolates of MRSA. A focused library of structural analogs was synthesized to explore initial structure-activity relationships and to identify equipotent myxocoumarin derivatives devoid of the natural nitro substituent to significantly streamline synthetic access. The cytotoxicity of the myxocoumarins as well as their potential to cure bacterial infections in vivo was established using a zebrafish model system. Our results reveal the exceptional antibiotic activity of the myxocoumarin scaffold and hence its potential for the development of novel antibiotics.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry-A European Journal",
title = "Strong Antibiotic Activity of the Myxocoumarin Scaffold in vitro and in vivo",
number = "32",
volume = "28",
doi = "10.1002/chem.202200394"
}

Hertrampf, G., Kusserow, K., Vojnović, S., Pavić, A., Mueller, J. I., Nikodinović-Runić, J.,& Gulder, T. A. M.. (2022). Strong Antibiotic Activity of the Myxocoumarin Scaffold in vitro and in vivo. in Chemistry-A European Journal
Wiley-V C H Verlag Gmbh, Weinheim., 28(32).
https://doi.org/10.1002/chem.202200394

Hertrampf G, Kusserow K, Vojnović S, Pavić A, Mueller JI, Nikodinović-Runić J, Gulder TAM. Strong Antibiotic Activity of the Myxocoumarin Scaffold in vitro and in vivo. in Chemistry-A European Journal. 2022;28(32).
doi:10.1002/chem.202200394 .

Hertrampf, Gesa, Kusserow, Kalina, Vojnović, Sandra, Pavić, Aleksandar, Mueller, Jonas, I, Nikodinović-Runić, Jasmina, Gulder, Tobias A. M., "Strong Antibiotic Activity of the Myxocoumarin Scaffold in vitro and in vivo" in Chemistry-A European Journal, 28, no. 32 (2022),
https://doi.org/10.1002/chem.202200394 . .

TY  - JOUR
AU  - Andrejević, Tina P.
AU  - Aleksić, Ivana
AU  - Kljun, Jakob
AU  - Pantović, Bojana, V
AU  - Milivojević, Dušan
AU  - Vojnović, Sandra
AU  - Turel, Iztok
AU  - Djuran, Milos
AU  - Glišić, Biljana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1589
AB  - Two zinc(II) complexes with dimethyl 2,2 '-bipyridine-4,5-dicarboxylate (py-2py) of the general formula [Zn(py-2py)X-2], X = Cl- (1) and Br- (2) were synthesized and characterized by NMR, IR and UV-Vis spectroscopy and single-crystal X-ray diffraction analysis. Complexes 1 and 2 are isostructural and adopt a slightly distorted tetrahedral geometry with values of tetrahedral indices tau(4) and tau'(4) in the range of 0.80-0.85. The complexes were evaluated for their in vitro antimicrobial activity against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two fungal strains (Candida albicans and Candida parapsilosis), while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5) and the model organism Caenorhabditis elegans. Complex 1 showed moderate activity against both Candida strains. However, this complex was twofold more cytotoxic compared to complex 2. The complexes tested had no effect on the survival rate of C. elegans. Complex 2 showed the ability to inhibit filamentation of C. albicans, while complex 1 was more effective than complex 2 in inhibiting biofilm formation. The interactions of complexes 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) were studied to evaluate their binding affinity toward these biomolecules.
PB  - MDPI, Basel
T2  - Inorganics
T1  - Zinc(II) Complexes with Dimethyl 2,2 '-Bipyridine-4,5-dicarboxylate: Structure, Antimicrobial Activity and DNA/BSA Binding Study
IS  - 6
VL  - 10
DO  - 10.3390/inorganics10060071
ER  - 

@article{
author = "Andrejević, Tina P. and Aleksić, Ivana and Kljun, Jakob and Pantović, Bojana, V and Milivojević, Dušan and Vojnović, Sandra and Turel, Iztok and Djuran, Milos and Glišić, Biljana",
year = "2022",
abstract = "Two zinc(II) complexes with dimethyl 2,2 '-bipyridine-4,5-dicarboxylate (py-2py) of the general formula [Zn(py-2py)X-2], X = Cl- (1) and Br- (2) were synthesized and characterized by NMR, IR and UV-Vis spectroscopy and single-crystal X-ray diffraction analysis. Complexes 1 and 2 are isostructural and adopt a slightly distorted tetrahedral geometry with values of tetrahedral indices tau(4) and tau'(4) in the range of 0.80-0.85. The complexes were evaluated for their in vitro antimicrobial activity against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two fungal strains (Candida albicans and Candida parapsilosis), while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5) and the model organism Caenorhabditis elegans. Complex 1 showed moderate activity against both Candida strains. However, this complex was twofold more cytotoxic compared to complex 2. The complexes tested had no effect on the survival rate of C. elegans. Complex 2 showed the ability to inhibit filamentation of C. albicans, while complex 1 was more effective than complex 2 in inhibiting biofilm formation. The interactions of complexes 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) were studied to evaluate their binding affinity toward these biomolecules.",
publisher = "MDPI, Basel",
journal = "Inorganics",
title = "Zinc(II) Complexes with Dimethyl 2,2 '-Bipyridine-4,5-dicarboxylate: Structure, Antimicrobial Activity and DNA/BSA Binding Study",
number = "6",
volume = "10",
doi = "10.3390/inorganics10060071"
}

Andrejević, T. P., Aleksić, I., Kljun, J., Pantović, B. V., Milivojević, D., Vojnović, S., Turel, I., Djuran, M.,& Glišić, B.. (2022). Zinc(II) Complexes with Dimethyl 2,2 '-Bipyridine-4,5-dicarboxylate: Structure, Antimicrobial Activity and DNA/BSA Binding Study. in Inorganics
MDPI, Basel., 10(6).
https://doi.org/10.3390/inorganics10060071

Andrejević TP, Aleksić I, Kljun J, Pantović BV, Milivojević D, Vojnović S, Turel I, Djuran M, Glišić B. Zinc(II) Complexes with Dimethyl 2,2 '-Bipyridine-4,5-dicarboxylate: Structure, Antimicrobial Activity and DNA/BSA Binding Study. in Inorganics. 2022;10(6).
doi:10.3390/inorganics10060071 .

Andrejević, Tina P., Aleksić, Ivana, Kljun, Jakob, Pantović, Bojana, V, Milivojević, Dušan, Vojnović, Sandra, Turel, Iztok, Djuran, Milos, Glišić, Biljana, "Zinc(II) Complexes with Dimethyl 2,2 '-Bipyridine-4,5-dicarboxylate: Structure, Antimicrobial Activity and DNA/BSA Binding Study" in Inorganics, 10, no. 6 (2022),
https://doi.org/10.3390/inorganics10060071 . .

TY  - JOUR
AU  - Lazić, Jelena
AU  - Škaro Bogojević, Sanja
AU  - Vojnović, Sandra
AU  - Aleksić, Ivana
AU  - Milivojević, Dušan
AU  - Kretzschmar, Martin
AU  - Gulder, Tanja
AU  - Petković, Milos
AU  - Nikodinović-Runić, Jasmina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1591
AB  - Prodigiosins (prodiginines) are a class of bacterial secondary metabolites with remarkable biological activities and color. In this study, optimized production, purification, and characterization of prodigiosin (PG) from easily accessible Serratia marcescens ATCC 27117 strain has been achieved to levels of 14 mg/L of culture within 24 h. Furthermore, environmentally friendly bromination of produced PG was used to afford both novel mono- and dibrominated derivatives of PG. PG and its Br derivatives showed anticancer potential with IC50 values range 0.62-17.00 mu g/mL for all tested cancer cell lines and induction of apoptosis but low selectivity against healthy cell lines. All compounds did not affect Caenorhabditis elegans at concentrations up to 50 mu g/mL. However, an improved toxicity profile of Br derivatives in comparison to parent PG was observed in vivo using zebrafish (Danio rerio) model system, when 10 mu g/mL applied at 6 h post fertilization caused death rate of 100%, 30% and 0% by PG, PG-Br, and PG-Br-2,Br- respectively, which is a significant finding for further structural optimizations of bacterial prodigiosins. The drug-likeness of PG and its Br derivatives was examined, and the novel Br derivatives obey the Lipinski's "rule of five", with an exemption of being more lipophilic than PG, which still makes them good targets for further structural optimization.
PB  - MDPI, Basel
T2  - Molecules
T1  - Synthesis, Anticancer Potential and Comprehensive Toxicity Studies of Novel Brominated Derivatives of Bacterial Biopigment Prodigiosin from Serratia marcescens ATCC 27117
IS  - 12
VL  - 27
DO  - 10.3390/molecules27123729
ER  - 

@article{
author = "Lazić, Jelena and Škaro Bogojević, Sanja and Vojnović, Sandra and Aleksić, Ivana and Milivojević, Dušan and Kretzschmar, Martin and Gulder, Tanja and Petković, Milos and Nikodinović-Runić, Jasmina",
year = "2022",
abstract = "Prodigiosins (prodiginines) are a class of bacterial secondary metabolites with remarkable biological activities and color. In this study, optimized production, purification, and characterization of prodigiosin (PG) from easily accessible Serratia marcescens ATCC 27117 strain has been achieved to levels of 14 mg/L of culture within 24 h. Furthermore, environmentally friendly bromination of produced PG was used to afford both novel mono- and dibrominated derivatives of PG. PG and its Br derivatives showed anticancer potential with IC50 values range 0.62-17.00 mu g/mL for all tested cancer cell lines and induction of apoptosis but low selectivity against healthy cell lines. All compounds did not affect Caenorhabditis elegans at concentrations up to 50 mu g/mL. However, an improved toxicity profile of Br derivatives in comparison to parent PG was observed in vivo using zebrafish (Danio rerio) model system, when 10 mu g/mL applied at 6 h post fertilization caused death rate of 100%, 30% and 0% by PG, PG-Br, and PG-Br-2,Br- respectively, which is a significant finding for further structural optimizations of bacterial prodigiosins. The drug-likeness of PG and its Br derivatives was examined, and the novel Br derivatives obey the Lipinski's "rule of five", with an exemption of being more lipophilic than PG, which still makes them good targets for further structural optimization.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Synthesis, Anticancer Potential and Comprehensive Toxicity Studies of Novel Brominated Derivatives of Bacterial Biopigment Prodigiosin from Serratia marcescens ATCC 27117",
number = "12",
volume = "27",
doi = "10.3390/molecules27123729"
}

Lazić, J., Škaro Bogojević, S., Vojnović, S., Aleksić, I., Milivojević, D., Kretzschmar, M., Gulder, T., Petković, M.,& Nikodinović-Runić, J.. (2022). Synthesis, Anticancer Potential and Comprehensive Toxicity Studies of Novel Brominated Derivatives of Bacterial Biopigment Prodigiosin from Serratia marcescens ATCC 27117. in Molecules
MDPI, Basel., 27(12).
https://doi.org/10.3390/molecules27123729

Lazić J, Škaro Bogojević S, Vojnović S, Aleksić I, Milivojević D, Kretzschmar M, Gulder T, Petković M, Nikodinović-Runić J. Synthesis, Anticancer Potential and Comprehensive Toxicity Studies of Novel Brominated Derivatives of Bacterial Biopigment Prodigiosin from Serratia marcescens ATCC 27117. in Molecules. 2022;27(12).
doi:10.3390/molecules27123729 .

Lazić, Jelena, Škaro Bogojević, Sanja, Vojnović, Sandra, Aleksić, Ivana, Milivojević, Dušan, Kretzschmar, Martin, Gulder, Tanja, Petković, Milos, Nikodinović-Runić, Jasmina, "Synthesis, Anticancer Potential and Comprehensive Toxicity Studies of Novel Brominated Derivatives of Bacterial Biopigment Prodigiosin from Serratia marcescens ATCC 27117" in Molecules, 27, no. 12 (2022),
https://doi.org/10.3390/molecules27123729 . .

TY  - JOUR
AU  - Keskin, Selbi
AU  - Dogan, Sengul Dilem
AU  - Gunduz, Miyase Gozde
AU  - Aleksić, Ivana
AU  - Vojnović, Sandra
AU  - Lazić, Jelena
AU  - Nikodinović-Runić, Jasmina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1514
AB  - In the present study, we report the synthesis, cytotoxicity determination and molecular modeling studies of twelve novel indole-based hydrazone derivatives ( IH1 - IH12 ). To obtain the target molecules, initially, 1 H -indole-2-carboxylic acid and ethanol were heated in the presence of an acid catalyst to yield ethyl 1 H -indole-2-carboxylate. Following the hydrazinolyzation of the ester moiety, the resulting compound, 1 H -indole-2-carbohydrazide reacted with appropriate benzaldehyde derivatives to obtain IH1 - IH12 . The proposed chemical structures of all compounds were confirmed by their 1 H NMR, 13 C NMR, IR, and HRMS data. Additionally, the configuration of C = N bond in IH8 was determined as ( E ) by applying 2D NMR technique, NOESY. Subsequently, the compounds were tested against both colon cancer (HCT116) and lung cancer (A549), as well as healthy lung fibroblast (MRC-5) cell lines to determine their potential as anticancer agents and their selectivity indexes (SI). Based on the obtained data from the antiproliferative MTT assay, HCT116 cell line was more sensitive to our molecules compared to A549. Furthermore, lipophilic halogens were preferable substituents on the phenyl ring for the selective toxicity against cancer cell lines. Drug-likeness analysis carried out by calculating important physicochemical properties of IH1 - IH12 confirmed that they all obey Lipinski's rule of five. Finally, hypoxia inducible factor (HIF)-1 alpha was suggested as the potential biological target of the compounds through molecular docking studies.
PB  - Elsevier, Amsterdam
T2  - Journal of Molecular Structure
T1  - Indole-based hydrazone derivatives: Synthesis, cytotoxicity assessment, and molecular modeling studies
VL  - 1270
DO  - 10.1016/j.molstruc.2022.133936
ER  - 

@article{
author = "Keskin, Selbi and Dogan, Sengul Dilem and Gunduz, Miyase Gozde and Aleksić, Ivana and Vojnović, Sandra and Lazić, Jelena and Nikodinović-Runić, Jasmina",
year = "2022",
abstract = "In the present study, we report the synthesis, cytotoxicity determination and molecular modeling studies of twelve novel indole-based hydrazone derivatives ( IH1 - IH12 ). To obtain the target molecules, initially, 1 H -indole-2-carboxylic acid and ethanol were heated in the presence of an acid catalyst to yield ethyl 1 H -indole-2-carboxylate. Following the hydrazinolyzation of the ester moiety, the resulting compound, 1 H -indole-2-carbohydrazide reacted with appropriate benzaldehyde derivatives to obtain IH1 - IH12 . The proposed chemical structures of all compounds were confirmed by their 1 H NMR, 13 C NMR, IR, and HRMS data. Additionally, the configuration of C = N bond in IH8 was determined as ( E ) by applying 2D NMR technique, NOESY. Subsequently, the compounds were tested against both colon cancer (HCT116) and lung cancer (A549), as well as healthy lung fibroblast (MRC-5) cell lines to determine their potential as anticancer agents and their selectivity indexes (SI). Based on the obtained data from the antiproliferative MTT assay, HCT116 cell line was more sensitive to our molecules compared to A549. Furthermore, lipophilic halogens were preferable substituents on the phenyl ring for the selective toxicity against cancer cell lines. Drug-likeness analysis carried out by calculating important physicochemical properties of IH1 - IH12 confirmed that they all obey Lipinski's rule of five. Finally, hypoxia inducible factor (HIF)-1 alpha was suggested as the potential biological target of the compounds through molecular docking studies.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Molecular Structure",
title = "Indole-based hydrazone derivatives: Synthesis, cytotoxicity assessment, and molecular modeling studies",
volume = "1270",
doi = "10.1016/j.molstruc.2022.133936"
}

Keskin, S., Dogan, S. D., Gunduz, M. G., Aleksić, I., Vojnović, S., Lazić, J.,& Nikodinović-Runić, J.. (2022). Indole-based hydrazone derivatives: Synthesis, cytotoxicity assessment, and molecular modeling studies. in Journal of Molecular Structure
Elsevier, Amsterdam., 1270.
https://doi.org/10.1016/j.molstruc.2022.133936

Keskin S, Dogan SD, Gunduz MG, Aleksić I, Vojnović S, Lazić J, Nikodinović-Runić J. Indole-based hydrazone derivatives: Synthesis, cytotoxicity assessment, and molecular modeling studies. in Journal of Molecular Structure. 2022;1270.
doi:10.1016/j.molstruc.2022.133936 .

Keskin, Selbi, Dogan, Sengul Dilem, Gunduz, Miyase Gozde, Aleksić, Ivana, Vojnović, Sandra, Lazić, Jelena, Nikodinović-Runić, Jasmina, "Indole-based hydrazone derivatives: Synthesis, cytotoxicity assessment, and molecular modeling studies" in Journal of Molecular Structure, 1270 (2022),
https://doi.org/10.1016/j.molstruc.2022.133936 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - D'Agostino, Paul M.
AU  - Mojicević, Marija
AU  - Gulder, Tobias A. M.
AU  - Nikodinović-Runić, Jasmina
AU  - Vojnović, Sandra
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1527
AB  - Aims Sequencing and genome analysis of two co-isolated streptomycetes, named BV410-1 and BV410-10, and the effect of their co-cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410-1 was 9.5 Mb, whilst that of BV410-10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410-1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410-10. The addition of cell-free supernatant from BV410-10 monoculture to BV410-1 fermentations improved the staurosporine yield from 8.35 mg L-1 up to 15.85 mg L-1, whilst BV410-10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410-1 and BV410-10 spore ratios. Conclusions The growth of BV410-10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410-10 showed potential to stimulate staurosporine production in BV410-1, but overall co-cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate. Significance and Impact of Study This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production
EP  - 2568
IS  - 4
SP  - 2560
VL  - 133
DO  - 10.1111/jam.15726
ER  - 

@article{
author = "Stevanović, Milena and D'Agostino, Paul M. and Mojicević, Marija and Gulder, Tobias A. M. and Nikodinović-Runić, Jasmina and Vojnović, Sandra",
year = "2022",
abstract = "Aims Sequencing and genome analysis of two co-isolated streptomycetes, named BV410-1 and BV410-10, and the effect of their co-cultivation on the staurosporine production. Methods and Results Identification of two strains through genome sequencing and their separation using different growth media was conducted. Sequence analysis revealed that the genome of BV410-1 was 9.5 Mb, whilst that of BV410-10 was 7.1 Mb. AntiSMASH analysis identified 28 biosynthetic gene clusters (BGCs) from BV410-1, including that responsible for staurosporine biosynthesis, whilst 20 BGCs were identified from BV410-10. The addition of cell-free supernatant from BV410-10 monoculture to BV410-1 fermentations improved the staurosporine yield from 8.35 mg L-1 up to 15.85 mg L-1, whilst BV410-10 monoculture ethyl acetate extract did not have the same effect. Also, there was no improvement in staurosporine production when artificial mixed cultures were created using three different BV410-1 and BV410-10 spore ratios. Conclusions The growth of BV410-10 was inhibited when the two strains were grown together on agar plates. Culture supernatants of BV410-10 showed potential to stimulate staurosporine production in BV410-1, but overall co-cultivation attempts did not restore the previously reported yield of staurosporine produced by the original mixed isolate. Significance and Impact of Study This work confirmed complex relations between streptomycetes in soil that are difficult to recreate under the laboratory conditions. Also, mining of streptomycetes genomes that mainly produce known bioactive compounds could still be the fruitful approach in search for novel bioactive molecules.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production",
pages = "2568-2560",
number = "4",
volume = "133",
doi = "10.1111/jam.15726"
}

Stevanović, M., D'Agostino, P. M., Mojicević, M., Gulder, T. A. M., Nikodinović-Runić, J.,& Vojnović, S.. (2022). Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production. in Journal of Applied Microbiology
Wiley, Hoboken., 133(4), 2560-2568.
https://doi.org/10.1111/jam.15726

Stevanović M, D'Agostino PM, Mojicević M, Gulder TAM, Nikodinović-Runić J, Vojnović S. Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production. in Journal of Applied Microbiology. 2022;133(4):2560-2568.
doi:10.1111/jam.15726 .

Stevanović, Milena, D'Agostino, Paul M., Mojicević, Marija, Gulder, Tobias A. M., Nikodinović-Runić, Jasmina, Vojnović, Sandra, "Streptomyces sp. BV410: Interspecies cross-talk for staurosporine production" in Journal of Applied Microbiology, 133, no. 4 (2022):2560-2568,
https://doi.org/10.1111/jam.15726 . .

TY  - JOUR
AU  - Simić, Milena R.
AU  - Erić, Slavica
AU  - Borić, Ivan
AU  - Lubelska, Annamaria
AU  - Latacz, Gniewomir
AU  - Kiec-Kononowicz, Katarzyna
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
AU  - Savić, Vladimir M.
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1498
AB  - In the continuation of our study of substituted isocoumarins a series of novel 3-azolyl isocoumarin and their thio derivatives, including some related lactone compounds was prepared and biologically profiled against C. albicans showing moderate activity with MIC values in range of 4-60 mu g mL(-1), in general. The additional characterisation of selected compounds was carried out by exploring their activity on CYP3A4 and CYP2D6 enzymes, while experiments on mutagenicity were performed by AMES test. The representative isocoumarins 3b, 4a and 4b showed lower inhibitory activity on CYP enzymes, when compared to the reference inhibitors, ketoconazole and quinidine. Compound 4a showed a higher mutagenic potential than the other two compounds. Further characterization included cytotoxicity profiling against normal MRC5 cells.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis and biological profiling of novel isocoumarin derivatives and related compounds
EP  - 649
IS  - 7-8
SP  - 639
VL  - 86
DO  - 10.2298/JSC201201025S
ER  - 

@article{
author = "Simić, Milena R. and Erić, Slavica and Borić, Ivan and Lubelska, Annamaria and Latacz, Gniewomir and Kiec-Kononowicz, Katarzyna and Vojnović, Sandra and Nikodinović-Runić, Jasmina and Savić, Vladimir M.",
year = "2021",
abstract = "In the continuation of our study of substituted isocoumarins a series of novel 3-azolyl isocoumarin and their thio derivatives, including some related lactone compounds was prepared and biologically profiled against C. albicans showing moderate activity with MIC values in range of 4-60 mu g mL(-1), in general. The additional characterisation of selected compounds was carried out by exploring their activity on CYP3A4 and CYP2D6 enzymes, while experiments on mutagenicity were performed by AMES test. The representative isocoumarins 3b, 4a and 4b showed lower inhibitory activity on CYP enzymes, when compared to the reference inhibitors, ketoconazole and quinidine. Compound 4a showed a higher mutagenic potential than the other two compounds. Further characterization included cytotoxicity profiling against normal MRC5 cells.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis and biological profiling of novel isocoumarin derivatives and related compounds",
pages = "649-639",
number = "7-8",
volume = "86",
doi = "10.2298/JSC201201025S"
}

Simić, M. R., Erić, S., Borić, I., Lubelska, A., Latacz, G., Kiec-Kononowicz, K., Vojnović, S., Nikodinović-Runić, J.,& Savić, V. M.. (2021). Synthesis and biological profiling of novel isocoumarin derivatives and related compounds. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 86(7-8), 639-649.
https://doi.org/10.2298/JSC201201025S

Simić MR, Erić S, Borić I, Lubelska A, Latacz G, Kiec-Kononowicz K, Vojnović S, Nikodinović-Runić J, Savić VM. Synthesis and biological profiling of novel isocoumarin derivatives and related compounds. in Journal of the Serbian Chemical Society. 2021;86(7-8):639-649.
doi:10.2298/JSC201201025S .

Simić, Milena R., Erić, Slavica, Borić, Ivan, Lubelska, Annamaria, Latacz, Gniewomir, Kiec-Kononowicz, Katarzyna, Vojnović, Sandra, Nikodinović-Runić, Jasmina, Savić, Vladimir M., "Synthesis and biological profiling of novel isocoumarin derivatives and related compounds" in Journal of the Serbian Chemical Society, 86, no. 7-8 (2021):639-649,
https://doi.org/10.2298/JSC201201025S . .

TY  - JOUR
AU  - Ferrandi, Erica E.
AU  - Spasić, Jelena
AU  - Đokić, Lidija
AU  - Vainshtein, Yevhen
AU  - Senthamaraikannan, Ramsankar
AU  - Vojnović, Sandra
AU  - Grumaz, Christian
AU  - Monti, Daniela
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1455
AB  - Three Streptomyces sp. strains with a multitude of target enzymatic activities confirmed by functional screening, namely BV129, BV286 and BV333, were subjected to genome sequencing aiming at the annotation of genes of interest, in-depth bioinformatics characterization and functional expression of the biocatalysts. A whole-genome shotgun sequencing followed by de novo genome assembly and annotation was performed revealing genomes of 6.4, 9.4 and 7.3 Mbp, respectively. Functional annotation of the proteins of interest resulted in between 2047 and 2763 putative targets. Among the various enzymatic activities that the three Streptomyces strains demonstrated to produce by functional screening, we focused our attention on transaminases (TAs) and laccases due to their high biocatalytic potential. Bioinformatics search allowed the identification of a putative TA from Streptomyces sp. BV333 as a potentially novel broad substrate scope TA and a putative laccase from Streptomyces sp. BV286 as potentially novel blue multicopper oxidase. The two sequences were cloned and overexpressed in Escherichia coli and the two novel enzymes, transaminase Sbv333-TA and laccase Sbv286-LAC, were characterized. Interestingly, both enzymes resulted to be exceptionally thermostable, Sbv333-TA showing a melting temperature (T-M = 85 degrees C) only slightly lower compared to the T-M of the most thermostable transaminases described to date (87-88 degrees C) and Sbv286-LAC being even thermoactivated at temperature  gt 60 degrees C. Moreover, Sbv333-TA showed a broad substrate scope and remarkably demonstrated to be active in the transamination of beta-ketoesters, which are rarely accepted by currently known TAs. On the other hand, Sbv286-LAC showed an improved activity in the presence of the cosolvent acetonitrile. Overall, it was shown that a combination of approaches from standard microbiological and biochemical screens to genome sequencing and analysis is required to afford novel and functional biocatalysts.
PB  - MDPI, Basel
T2  - Catalysts
T1  - Novel Transaminase and Laccase from Streptomyces spp. Using Combined Identification Approaches
IS  - 8
VL  - 11
DO  - 10.3390/catal11080919
ER  - 

@article{
author = "Ferrandi, Erica E. and Spasić, Jelena and Đokić, Lidija and Vainshtein, Yevhen and Senthamaraikannan, Ramsankar and Vojnović, Sandra and Grumaz, Christian and Monti, Daniela and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Three Streptomyces sp. strains with a multitude of target enzymatic activities confirmed by functional screening, namely BV129, BV286 and BV333, were subjected to genome sequencing aiming at the annotation of genes of interest, in-depth bioinformatics characterization and functional expression of the biocatalysts. A whole-genome shotgun sequencing followed by de novo genome assembly and annotation was performed revealing genomes of 6.4, 9.4 and 7.3 Mbp, respectively. Functional annotation of the proteins of interest resulted in between 2047 and 2763 putative targets. Among the various enzymatic activities that the three Streptomyces strains demonstrated to produce by functional screening, we focused our attention on transaminases (TAs) and laccases due to their high biocatalytic potential. Bioinformatics search allowed the identification of a putative TA from Streptomyces sp. BV333 as a potentially novel broad substrate scope TA and a putative laccase from Streptomyces sp. BV286 as potentially novel blue multicopper oxidase. The two sequences were cloned and overexpressed in Escherichia coli and the two novel enzymes, transaminase Sbv333-TA and laccase Sbv286-LAC, were characterized. Interestingly, both enzymes resulted to be exceptionally thermostable, Sbv333-TA showing a melting temperature (T-M = 85 degrees C) only slightly lower compared to the T-M of the most thermostable transaminases described to date (87-88 degrees C) and Sbv286-LAC being even thermoactivated at temperature  gt 60 degrees C. Moreover, Sbv333-TA showed a broad substrate scope and remarkably demonstrated to be active in the transamination of beta-ketoesters, which are rarely accepted by currently known TAs. On the other hand, Sbv286-LAC showed an improved activity in the presence of the cosolvent acetonitrile. Overall, it was shown that a combination of approaches from standard microbiological and biochemical screens to genome sequencing and analysis is required to afford novel and functional biocatalysts.",
publisher = "MDPI, Basel",
journal = "Catalysts",
title = "Novel Transaminase and Laccase from Streptomyces spp. Using Combined Identification Approaches",
number = "8",
volume = "11",
doi = "10.3390/catal11080919"
}

Ferrandi, E. E., Spasić, J., Đokić, L., Vainshtein, Y., Senthamaraikannan, R., Vojnović, S., Grumaz, C., Monti, D.,& Nikodinović-Runić, J.. (2021). Novel Transaminase and Laccase from Streptomyces spp. Using Combined Identification Approaches. in Catalysts
MDPI, Basel., 11(8).
https://doi.org/10.3390/catal11080919

Ferrandi EE, Spasić J, Đokić L, Vainshtein Y, Senthamaraikannan R, Vojnović S, Grumaz C, Monti D, Nikodinović-Runić J. Novel Transaminase and Laccase from Streptomyces spp. Using Combined Identification Approaches. in Catalysts. 2021;11(8).
doi:10.3390/catal11080919 .

Ferrandi, Erica E., Spasić, Jelena, Đokić, Lidija, Vainshtein, Yevhen, Senthamaraikannan, Ramsankar, Vojnović, Sandra, Grumaz, Christian, Monti, Daniela, Nikodinović-Runić, Jasmina, "Novel Transaminase and Laccase from Streptomyces spp. Using Combined Identification Approaches" in Catalysts, 11, no. 8 (2021),
https://doi.org/10.3390/catal11080919 . .