Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness

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Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (en)
Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (sr)
Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (sr_RS)
Authors

Publications

Chokeberry polyphenols preservation using spray drying: effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion

Cujić-Nikolić, Nada; Stanisavljević, Nemanja; Savikin, Katarina; Kaluević, Ana; Nedović, Viktor; Samardžić, Jelena; Janković, Teodora

(Taylor & Francis Ltd, Abingdon, 2019)

TY  - JOUR
AU  - Cujić-Nikolić, Nada
AU  - Stanisavljević, Nemanja
AU  - Savikin, Katarina
AU  - Kaluević, Ana
AU  - Nedović, Viktor
AU  - Samardžić, Jelena
AU  - Janković, Teodora
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1215
AB  - Aim: Microencapsulation of chokeberry extracts was performed in order to improve functionality, stability, and bioavailability of extracted polyphenols. Methods: Chokeberry fruits and juice by-product (waste) extracts were spray-dried by using two carriers, maltodextrin and skimmed milk. Morphological and physicochemical characteristics of the obtained powders were analysed. In vitro simulated digestion model was used as an indicator of polyphenolics bioavailability. Results: The moisture content varied between 3.39 and 4.61%, zeta potential had negative values (35-39 mV), maltodetrin powders were smaller (4.27-5.12 mu m) compared to skimmed ones (8.50-11.01 mu m). All microparticles exhibited high encapsulation efficiency of total polyphenols and anthocyanins (73-97% and 63-96%, respectively). For both extract types, maltodextrin powders released higher phenolics content compared to skimmed milk. During in vitro digestion, maltodextrin exhibited a higher protective effect on both active compounds. Conclusion: Taking into account the obtained results, chokeberry polyphenols stability might be improved using spray drying technique, and maltodextrin showed better properties.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Microencapsulation
T1  - Chokeberry polyphenols preservation using spray drying: effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion
EP  - 703
IS  - 8
SP  - 693
VL  - 36
DO  - 10.1080/02652048.2019.1667448
ER  - 
@article{
author = "Cujić-Nikolić, Nada and Stanisavljević, Nemanja and Savikin, Katarina and Kaluević, Ana and Nedović, Viktor and Samardžić, Jelena and Janković, Teodora",
year = "2019",
abstract = "Aim: Microencapsulation of chokeberry extracts was performed in order to improve functionality, stability, and bioavailability of extracted polyphenols. Methods: Chokeberry fruits and juice by-product (waste) extracts were spray-dried by using two carriers, maltodextrin and skimmed milk. Morphological and physicochemical characteristics of the obtained powders were analysed. In vitro simulated digestion model was used as an indicator of polyphenolics bioavailability. Results: The moisture content varied between 3.39 and 4.61%, zeta potential had negative values (35-39 mV), maltodetrin powders were smaller (4.27-5.12 mu m) compared to skimmed ones (8.50-11.01 mu m). All microparticles exhibited high encapsulation efficiency of total polyphenols and anthocyanins (73-97% and 63-96%, respectively). For both extract types, maltodextrin powders released higher phenolics content compared to skimmed milk. During in vitro digestion, maltodextrin exhibited a higher protective effect on both active compounds. Conclusion: Taking into account the obtained results, chokeberry polyphenols stability might be improved using spray drying technique, and maltodextrin showed better properties.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Microencapsulation",
title = "Chokeberry polyphenols preservation using spray drying: effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion",
pages = "703-693",
number = "8",
volume = "36",
doi = "10.1080/02652048.2019.1667448"
}
Cujić-Nikolić, N., Stanisavljević, N., Savikin, K., Kaluević, A., Nedović, V., Samardžić, J.,& Janković, T.. (2019). Chokeberry polyphenols preservation using spray drying: effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion. in Journal of Microencapsulation
Taylor & Francis Ltd, Abingdon., 36(8), 693-703.
https://doi.org/10.1080/02652048.2019.1667448
Cujić-Nikolić N, Stanisavljević N, Savikin K, Kaluević A, Nedović V, Samardžić J, Janković T. Chokeberry polyphenols preservation using spray drying: effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion. in Journal of Microencapsulation. 2019;36(8):693-703.
doi:10.1080/02652048.2019.1667448 .
Cujić-Nikolić, Nada, Stanisavljević, Nemanja, Savikin, Katarina, Kaluević, Ana, Nedović, Viktor, Samardžić, Jelena, Janković, Teodora, "Chokeberry polyphenols preservation using spray drying: effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion" in Journal of Microencapsulation, 36, no. 8 (2019):693-703,
https://doi.org/10.1080/02652048.2019.1667448 . .
43
8
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The Characterization of the Selected Trees Damaged During Severe Weather Episode on the Mountain Avala (Serbia) Using IR Thermography, ICP-OES, and Microbiological Analysis

Alagić, Sladana; Stević, Zoran Miroslava; Jovanić, Predrag Branko; Morić, Ivana; Jeremić, Sanja; Popara, Ljubomir Blagoje

(Springer International Publishing Ag, Cham, 2018)

TY  - JOUR
AU  - Alagić, Sladana
AU  - Stević, Zoran Miroslava
AU  - Jovanić, Predrag Branko
AU  - Morić, Ivana
AU  - Jeremić, Sanja
AU  - Popara, Ljubomir Blagoje
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1183
AB  - Selected plants of white fir and lime, damaged during severe weather episode on the mountain Avala (Serbia) in summer 2014, were analyzed and characterized (including their spatial soil samples) by inductively coupled plasma optical emission spectroscopy (ICP-OES), infrared (IR) thermography, and microbiological method such as enumeration of cultivable microorganisms. The results obtained from chemical and microbiological analyses provided valuable information on possible biotic and abiotic stressors such as soil fungi and heavy metals, which could affect the health status of trees, while IR thermography visualized this status in a very specific and effective way. The results of ICP-OES analysis clearly showed that the investigated heavy metals (Cu, Zn, Pb, As, Cd, and Ni) were less likely crucial factors responsible for ruined health status of damaged trees. The role of soil fungi was not clear, since the results of microbiological analysis only provided evidence that their amounts in all investigated soil samples were within normal ranges as well as that their amounts in the corresponding samples of the uprooted trees were much greater than in the case of snapped trees. Therefore, further molecular characterization of microorganisms should be performed to identify if pathogenic species are present and clarify their role. Nevertheless, all used methods, especially IR thermal imaging as a totally non-invasive, fast and very comfortable technique, can be recommended as very useful in preventive screening of the trees' health status and for early detection of tissue decay that usually hamper trees survival or resistance to extreme weather events.
PB  - Springer International Publishing Ag, Cham
T2  - International Journal of Environmental Research
T1  - The Characterization of the Selected Trees Damaged During Severe Weather Episode on the Mountain Avala (Serbia) Using IR Thermography, ICP-OES, and Microbiological Analysis
EP  - 146
IS  - 2
SP  - 135
VL  - 12
DO  - 10.1007/s41742-018-0078-y
ER  - 
@article{
author = "Alagić, Sladana and Stević, Zoran Miroslava and Jovanić, Predrag Branko and Morić, Ivana and Jeremić, Sanja and Popara, Ljubomir Blagoje",
year = "2018",
abstract = "Selected plants of white fir and lime, damaged during severe weather episode on the mountain Avala (Serbia) in summer 2014, were analyzed and characterized (including their spatial soil samples) by inductively coupled plasma optical emission spectroscopy (ICP-OES), infrared (IR) thermography, and microbiological method such as enumeration of cultivable microorganisms. The results obtained from chemical and microbiological analyses provided valuable information on possible biotic and abiotic stressors such as soil fungi and heavy metals, which could affect the health status of trees, while IR thermography visualized this status in a very specific and effective way. The results of ICP-OES analysis clearly showed that the investigated heavy metals (Cu, Zn, Pb, As, Cd, and Ni) were less likely crucial factors responsible for ruined health status of damaged trees. The role of soil fungi was not clear, since the results of microbiological analysis only provided evidence that their amounts in all investigated soil samples were within normal ranges as well as that their amounts in the corresponding samples of the uprooted trees were much greater than in the case of snapped trees. Therefore, further molecular characterization of microorganisms should be performed to identify if pathogenic species are present and clarify their role. Nevertheless, all used methods, especially IR thermal imaging as a totally non-invasive, fast and very comfortable technique, can be recommended as very useful in preventive screening of the trees' health status and for early detection of tissue decay that usually hamper trees survival or resistance to extreme weather events.",
publisher = "Springer International Publishing Ag, Cham",
journal = "International Journal of Environmental Research",
title = "The Characterization of the Selected Trees Damaged During Severe Weather Episode on the Mountain Avala (Serbia) Using IR Thermography, ICP-OES, and Microbiological Analysis",
pages = "146-135",
number = "2",
volume = "12",
doi = "10.1007/s41742-018-0078-y"
}
Alagić, S., Stević, Z. M., Jovanić, P. B., Morić, I., Jeremić, S.,& Popara, L. B.. (2018). The Characterization of the Selected Trees Damaged During Severe Weather Episode on the Mountain Avala (Serbia) Using IR Thermography, ICP-OES, and Microbiological Analysis. in International Journal of Environmental Research
Springer International Publishing Ag, Cham., 12(2), 135-146.
https://doi.org/10.1007/s41742-018-0078-y
Alagić S, Stević ZM, Jovanić PB, Morić I, Jeremić S, Popara LB. The Characterization of the Selected Trees Damaged During Severe Weather Episode on the Mountain Avala (Serbia) Using IR Thermography, ICP-OES, and Microbiological Analysis. in International Journal of Environmental Research. 2018;12(2):135-146.
doi:10.1007/s41742-018-0078-y .
Alagić, Sladana, Stević, Zoran Miroslava, Jovanić, Predrag Branko, Morić, Ivana, Jeremić, Sanja, Popara, Ljubomir Blagoje, "The Characterization of the Selected Trees Damaged During Severe Weather Episode on the Mountain Avala (Serbia) Using IR Thermography, ICP-OES, and Microbiological Analysis" in International Journal of Environmental Research, 12, no. 2 (2018):135-146,
https://doi.org/10.1007/s41742-018-0078-y . .

Antibacterial potential of electrochemically exfoliated graphene sheets

Marković, Zoran M.; Matijašević, Danka; Pavlović, Vladimir B.; Jovanović, Svetlana P.; Holclajtner-Antunović, Ivanka D.; Spitalsky, Zdenko; Micusik, Matej; Dramicanin, Miroslav D.; Milivojević, Dusan D.; Nikšić, Miomir; Marković, Biljana M. Todorovic

(Academic Press Inc Elsevier Science, San Diego, 2017)

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Matijašević, Danka
AU  - Pavlović, Vladimir B.
AU  - Jovanović, Svetlana P.
AU  - Holclajtner-Antunović, Ivanka D.
AU  - Spitalsky, Zdenko
AU  - Micusik, Matej
AU  - Dramicanin, Miroslav D.
AU  - Milivojević, Dusan D.
AU  - Nikšić, Miomir
AU  - Marković, Biljana M. Todorovic
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1090
AB  - Electrochemically exfoliated graphene is functionalized graphene with potential application in biomedicine. Two most relevant biological features of this material are its electrical conductivity and excellent water dispersibility. In this study we have tried to establish the correlation between graphene structure and its antibacterial properties. The exfoliation process was performed in a two electrode-highly oriented pyrolytic graphite electrochemical cell. Solution of ammonium persulfate was used as an electrolyte. Exfoliated graphene sheets were dispersed in aqueous media and characterized by atomic force microscopy, scanning electron microscopy, Raman spectroscopy, Fourier transform infrared spectroscopy, X photoelectron spectroscopy, X-ray diffraction, electron paramagnetic resonance, zeta potential, contact angle measurements and surface energy. Antibacterial assays have shown lack of the significant antibacterial activity. Major effect on bacteria was slight change of bacteria morphology. Membrane remained intact despite significant change of chemical content of membrane components.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Colloid and Interface Science
T1  - Antibacterial potential of electrochemically exfoliated graphene sheets
EP  - 43
SP  - 30
VL  - 500
DO  - 10.1016/j.jcis.2017.03.110
ER  - 
@article{
author = "Marković, Zoran M. and Matijašević, Danka and Pavlović, Vladimir B. and Jovanović, Svetlana P. and Holclajtner-Antunović, Ivanka D. and Spitalsky, Zdenko and Micusik, Matej and Dramicanin, Miroslav D. and Milivojević, Dusan D. and Nikšić, Miomir and Marković, Biljana M. Todorovic",
year = "2017",
abstract = "Electrochemically exfoliated graphene is functionalized graphene with potential application in biomedicine. Two most relevant biological features of this material are its electrical conductivity and excellent water dispersibility. In this study we have tried to establish the correlation between graphene structure and its antibacterial properties. The exfoliation process was performed in a two electrode-highly oriented pyrolytic graphite electrochemical cell. Solution of ammonium persulfate was used as an electrolyte. Exfoliated graphene sheets were dispersed in aqueous media and characterized by atomic force microscopy, scanning electron microscopy, Raman spectroscopy, Fourier transform infrared spectroscopy, X photoelectron spectroscopy, X-ray diffraction, electron paramagnetic resonance, zeta potential, contact angle measurements and surface energy. Antibacterial assays have shown lack of the significant antibacterial activity. Major effect on bacteria was slight change of bacteria morphology. Membrane remained intact despite significant change of chemical content of membrane components.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Colloid and Interface Science",
title = "Antibacterial potential of electrochemically exfoliated graphene sheets",
pages = "43-30",
volume = "500",
doi = "10.1016/j.jcis.2017.03.110"
}
Marković, Z. M., Matijašević, D., Pavlović, V. B., Jovanović, S. P., Holclajtner-Antunović, I. D., Spitalsky, Z., Micusik, M., Dramicanin, M. D., Milivojević, D. D., Nikšić, M.,& Marković, B. M. T.. (2017). Antibacterial potential of electrochemically exfoliated graphene sheets. in Journal of Colloid and Interface Science
Academic Press Inc Elsevier Science, San Diego., 500, 30-43.
https://doi.org/10.1016/j.jcis.2017.03.110
Marković ZM, Matijašević D, Pavlović VB, Jovanović SP, Holclajtner-Antunović ID, Spitalsky Z, Micusik M, Dramicanin MD, Milivojević DD, Nikšić M, Marković BMT. Antibacterial potential of electrochemically exfoliated graphene sheets. in Journal of Colloid and Interface Science. 2017;500:30-43.
doi:10.1016/j.jcis.2017.03.110 .
Marković, Zoran M., Matijašević, Danka, Pavlović, Vladimir B., Jovanović, Svetlana P., Holclajtner-Antunović, Ivanka D., Spitalsky, Zdenko, Micusik, Matej, Dramicanin, Miroslav D., Milivojević, Dusan D., Nikšić, Miomir, Marković, Biljana M. Todorovic, "Antibacterial potential of electrochemically exfoliated graphene sheets" in Journal of Colloid and Interface Science, 500 (2017):30-43,
https://doi.org/10.1016/j.jcis.2017.03.110 . .
9
33
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Uticaj Se(IV)- i Se(VI)- modifikovanog zeolita na antioksidativno i antimikrobno dejstvo gljiva Pleurotus ostreatus i Coriolus versicolor

Matijašević, Danka

(Univerzitet u Beogradu, Poljoprivredni fakultet, 2017)

TY  - THES
AU  - Matijašević, Danka
PY  - 2017
UR  - https://nardus.mpn.gov.rs/handle/123456789/8619
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5291
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:16341/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49365519
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/45
AB  - Poslednjih nekoliko decenija sve veća pažnja se posvećuje nutritivnim i medicinskim svojstvima gljiva. Gljive mogu transformisati biljne lignocelulozne ostatke niske hranljive vrednosti u visoko vredne delikatesne namirnice. Dokazano je i da mogu usvojiti selen, iz supstrata na kojem rastu, i da ta sposobnost zavisi od vrste gljive, kao i od oblika i koncentracije dodatog selena. Selen je esencijalni mikronutrijent, neophodan za normalno funkcionisanje organizma. Istraživanja o zastupljenosti ovog elementa ukazuju da zemljište Srbije, kao i životne namirnice spadaju među selenom najsiromašnije u svetu. Sa druge strane, Srbija raspolaže značajnim količinama zeolita i procenjuje se da se najveće zalihe ovog minerala u Evropi nalaze u blizini Vranjske Banje. Zeolitski tuf može biti modifikovan i potom upotrebljen kao adsorbent selenit- i selenat-jona. Uticaj Se(IV)- i Se(VI)-modifikovanog zeolita (SeIV-CLI i SeVI-CLI) na aktivnost enzima antioksidativnog sistema zaštite praćen je u svežim plodonosnim telima gljiva Pleurotus ostreatus i Coriolus versicolor. Jača aktivnost enzima superoksid-dismutaze i glutation-peroksidaze je utvrđena u svim uzorcima gljive P. ostreatus odgajenim na supstratu sa dodatkom selena u odnosu na kontrolu. Kod većine uzoraka gljive C. versicolor, obogaćene selenom, ustanovljena je jača aktivnost enzima superoksid-dismutaze i katalaze i slabija aktivnost glutation-peroksidaze u poređenju sa kontrolom. Primenom SDS-PAGE gel elektroforeze utvrđeno je da selen prisutan u uzorcima nije uticao na promenu molekulskih masa ispitivanih gljiva. U ovoj disertaciji izolovani su metanolni ekstrakti gljiva P. ostreatus i C. versicolor, odgajenih na supstratu bez i sa dodatkom različitih koncentracija selena u vidu Se(IV)- i Se(VI)-modifikovanog zeolita. Sadržaj selena u ekstraktima pripremljenim iz gljive P. ostreatus, obogaćene ovim elementom, je bio od 35–125 puta veći u poređenju sa kontrolnim ekstraktom, dok je kod gljive C. versicolor određen od 180–250 puta veći sadržaj selena...
AB  - In recent decades, more attention is paid to the nutritional and medicinal properties of fungi. Fungi can transform lignocellulosic residues of plants with low nutritional value into high-value delicious food. It has been shown that fungi can absorb selenium from the growing substrate and that this ability depends on the type of fungus and concentration of the added selenium. Selenium is an essential micronutient, necessary for the proper functioning of the organism. Research on the distribution of this element indicates that land in Serbia, and foodstuffs are among the poorest in the world. On the other hand, Serbia has significant quantities of zeolite and it is estimated that the largest reserves of this mineral in Europe are located near Vranjska Banja. The zeolitic tuff can be modified and then used as an adsorbent for selenite- and selenate- ions. The influence of Se(IV)- and Se(VI)- modified zeolite (SeVI-CLI and SeVI-CLI) on the enzyme activity of the antioxidant protection system was monitored in fresh fruiting bodies of the fungi P. ostreatus and C. versicolor. Increased activity of the superoxide dismutase and glutathione peroxidase enzymes was measured in all samples of P. ostreatus grown on the substrate with the addition of selenium compared to the control samples. Most samples of C. versicolor enriched with selenium showed increased activity of superoxide dismutase and catalase enzymes and weaker activity of glutathione peroxidase compared to the control. By applying SDS-PAGE gel electrophoresis it was found that selenium present in the samples did not affect the molecular weight change of the tested fungi. In this dissertation methanol extracts of fungi P. ostreatus and C. versicolor grown on a substrate with and without addition of various concentrations of selenium in the form of Se(IV)- and Se(VI)-modified zeolite were isolated...
PB  - Univerzitet u Beogradu, Poljoprivredni fakultet
T1  - Uticaj Se(IV)- i Se(VI)- modifikovanog zeolita na antioksidativno i antimikrobno dejstvo gljiva Pleurotus ostreatus i Coriolus versicolor
T1  - The influence OF Se(IV)- and Se(VI)- modified zeolite on antioxidant and antimicrobial activity of fungi Pleurotus ostreatus and Coriolus versicolor
UR  - https://hdl.handle.net/21.15107/rcub_nardus_8619
ER  - 
@phdthesis{
author = "Matijašević, Danka",
year = "2017",
abstract = "Poslednjih nekoliko decenija sve veća pažnja se posvećuje nutritivnim i medicinskim svojstvima gljiva. Gljive mogu transformisati biljne lignocelulozne ostatke niske hranljive vrednosti u visoko vredne delikatesne namirnice. Dokazano je i da mogu usvojiti selen, iz supstrata na kojem rastu, i da ta sposobnost zavisi od vrste gljive, kao i od oblika i koncentracije dodatog selena. Selen je esencijalni mikronutrijent, neophodan za normalno funkcionisanje organizma. Istraživanja o zastupljenosti ovog elementa ukazuju da zemljište Srbije, kao i životne namirnice spadaju među selenom najsiromašnije u svetu. Sa druge strane, Srbija raspolaže značajnim količinama zeolita i procenjuje se da se najveće zalihe ovog minerala u Evropi nalaze u blizini Vranjske Banje. Zeolitski tuf može biti modifikovan i potom upotrebljen kao adsorbent selenit- i selenat-jona. Uticaj Se(IV)- i Se(VI)-modifikovanog zeolita (SeIV-CLI i SeVI-CLI) na aktivnost enzima antioksidativnog sistema zaštite praćen je u svežim plodonosnim telima gljiva Pleurotus ostreatus i Coriolus versicolor. Jača aktivnost enzima superoksid-dismutaze i glutation-peroksidaze je utvrđena u svim uzorcima gljive P. ostreatus odgajenim na supstratu sa dodatkom selena u odnosu na kontrolu. Kod većine uzoraka gljive C. versicolor, obogaćene selenom, ustanovljena je jača aktivnost enzima superoksid-dismutaze i katalaze i slabija aktivnost glutation-peroksidaze u poređenju sa kontrolom. Primenom SDS-PAGE gel elektroforeze utvrđeno je da selen prisutan u uzorcima nije uticao na promenu molekulskih masa ispitivanih gljiva. U ovoj disertaciji izolovani su metanolni ekstrakti gljiva P. ostreatus i C. versicolor, odgajenih na supstratu bez i sa dodatkom različitih koncentracija selena u vidu Se(IV)- i Se(VI)-modifikovanog zeolita. Sadržaj selena u ekstraktima pripremljenim iz gljive P. ostreatus, obogaćene ovim elementom, je bio od 35–125 puta veći u poređenju sa kontrolnim ekstraktom, dok je kod gljive C. versicolor određen od 180–250 puta veći sadržaj selena..., In recent decades, more attention is paid to the nutritional and medicinal properties of fungi. Fungi can transform lignocellulosic residues of plants with low nutritional value into high-value delicious food. It has been shown that fungi can absorb selenium from the growing substrate and that this ability depends on the type of fungus and concentration of the added selenium. Selenium is an essential micronutient, necessary for the proper functioning of the organism. Research on the distribution of this element indicates that land in Serbia, and foodstuffs are among the poorest in the world. On the other hand, Serbia has significant quantities of zeolite and it is estimated that the largest reserves of this mineral in Europe are located near Vranjska Banja. The zeolitic tuff can be modified and then used as an adsorbent for selenite- and selenate- ions. The influence of Se(IV)- and Se(VI)- modified zeolite (SeVI-CLI and SeVI-CLI) on the enzyme activity of the antioxidant protection system was monitored in fresh fruiting bodies of the fungi P. ostreatus and C. versicolor. Increased activity of the superoxide dismutase and glutathione peroxidase enzymes was measured in all samples of P. ostreatus grown on the substrate with the addition of selenium compared to the control samples. Most samples of C. versicolor enriched with selenium showed increased activity of superoxide dismutase and catalase enzymes and weaker activity of glutathione peroxidase compared to the control. By applying SDS-PAGE gel electrophoresis it was found that selenium present in the samples did not affect the molecular weight change of the tested fungi. In this dissertation methanol extracts of fungi P. ostreatus and C. versicolor grown on a substrate with and without addition of various concentrations of selenium in the form of Se(IV)- and Se(VI)-modified zeolite were isolated...",
publisher = "Univerzitet u Beogradu, Poljoprivredni fakultet",
title = "Uticaj Se(IV)- i Se(VI)- modifikovanog zeolita na antioksidativno i antimikrobno dejstvo gljiva Pleurotus ostreatus i Coriolus versicolor, The influence OF Se(IV)- and Se(VI)- modified zeolite on antioxidant and antimicrobial activity of fungi Pleurotus ostreatus and Coriolus versicolor",
url = "https://hdl.handle.net/21.15107/rcub_nardus_8619"
}
Matijašević, D.. (2017). Uticaj Se(IV)- i Se(VI)- modifikovanog zeolita na antioksidativno i antimikrobno dejstvo gljiva Pleurotus ostreatus i Coriolus versicolor. 
Univerzitet u Beogradu, Poljoprivredni fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_8619
Matijašević D. Uticaj Se(IV)- i Se(VI)- modifikovanog zeolita na antioksidativno i antimikrobno dejstvo gljiva Pleurotus ostreatus i Coriolus versicolor. 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_8619 .
Matijašević, Danka, "Uticaj Se(IV)- i Se(VI)- modifikovanog zeolita na antioksidativno i antimikrobno dejstvo gljiva Pleurotus ostreatus i Coriolus versicolor" (2017),
https://hdl.handle.net/21.15107/rcub_nardus_8619 .

Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes

Lukić, Jovanka; Jancić, I.; Mirković, N.; Bufan, B.; Đokić, Jelena; Milenković, M.; Begović, Jelena; Strahinić, Ivana; Lozo, Jelena

(Wageningen Academic Publishers, Wageningen, 2017)

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Jancić, I.
AU  - Mirković, N.
AU  - Bufan, B.
AU  - Đokić, Jelena
AU  - Milenković, M.
AU  - Begović, Jelena
AU  - Strahinić, Ivana
AU  - Lozo, Jelena
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1003
AB  - In the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. Accordingly, the aim of this study was to test whether two lactic acid bacteria (LAB) strains, Lactococcus lactis BGBU1-4 and Lactobacillus salivarius BGHO1, could be applied as safe supplements for Listeria infection. Two major research objectives were set: to compare the effects of BGBU1-4 and BGHO1 on early immune response in gut tissue of Wistar rats co-administered with Listeria monocytogenes ATCC19111 and next, to test how this applies to their usage as therapeutics in acute ATCC19111 infection. Intestinal villi (IV), Peyer's patches (PP) and mesenteric lymph nodes (MLN) were used for the analysis. The results showed that BGHO1 increased the mRNA expression of innate immune markers CD14, interleukin (IL)-1 beta and tumour necrosis factor (TNF)-alpha in PP and IV, and, in parallel, caused a decrease of listeriolysin O (LLO) mRNA expression in same tissues. In MLN of BGHO1 treated rats, LLO expression was increased, along with an increase of the expression of OX-62 mRNA and CD69, pointing to the activation of adaptive immunity. On the other hand, in BGBU1-4 treated rats, there was no reduction of LLO mRNA expression and no induction of innate immunity markers in intestinal tissue. Additionally, CD14 and IL-1 beta, as well as LLO, but not OX-62 mRNA and CD69 expression, were elevated in MLN of BGBU1-4 treated rats. However, when applied therapeutically, both, BGBU1-4 and BGHO1, lowered Listeria count in spleens of infected rats. Our results not only reveal the potential of LAB to ameliorate Listeria infections, but suggest different immunological effects of two different LAB strains, both of which could be effective in Listeria elimination.
PB  - Wageningen Academic Publishers, Wageningen
T2  - Beneficial Microbes
T1  - Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes
EP  - 822
IS  - 5
SP  - 809
VL  - 8
DO  - 10.3920/BM2017.0007
ER  - 
@article{
author = "Lukić, Jovanka and Jancić, I. and Mirković, N. and Bufan, B. and Đokić, Jelena and Milenković, M. and Begović, Jelena and Strahinić, Ivana and Lozo, Jelena",
year = "2017",
abstract = "In the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. Accordingly, the aim of this study was to test whether two lactic acid bacteria (LAB) strains, Lactococcus lactis BGBU1-4 and Lactobacillus salivarius BGHO1, could be applied as safe supplements for Listeria infection. Two major research objectives were set: to compare the effects of BGBU1-4 and BGHO1 on early immune response in gut tissue of Wistar rats co-administered with Listeria monocytogenes ATCC19111 and next, to test how this applies to their usage as therapeutics in acute ATCC19111 infection. Intestinal villi (IV), Peyer's patches (PP) and mesenteric lymph nodes (MLN) were used for the analysis. The results showed that BGHO1 increased the mRNA expression of innate immune markers CD14, interleukin (IL)-1 beta and tumour necrosis factor (TNF)-alpha in PP and IV, and, in parallel, caused a decrease of listeriolysin O (LLO) mRNA expression in same tissues. In MLN of BGHO1 treated rats, LLO expression was increased, along with an increase of the expression of OX-62 mRNA and CD69, pointing to the activation of adaptive immunity. On the other hand, in BGBU1-4 treated rats, there was no reduction of LLO mRNA expression and no induction of innate immunity markers in intestinal tissue. Additionally, CD14 and IL-1 beta, as well as LLO, but not OX-62 mRNA and CD69 expression, were elevated in MLN of BGBU1-4 treated rats. However, when applied therapeutically, both, BGBU1-4 and BGHO1, lowered Listeria count in spleens of infected rats. Our results not only reveal the potential of LAB to ameliorate Listeria infections, but suggest different immunological effects of two different LAB strains, both of which could be effective in Listeria elimination.",
publisher = "Wageningen Academic Publishers, Wageningen",
journal = "Beneficial Microbes",
title = "Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes",
pages = "822-809",
number = "5",
volume = "8",
doi = "10.3920/BM2017.0007"
}
Lukić, J., Jancić, I., Mirković, N., Bufan, B., Đokić, J., Milenković, M., Begović, J., Strahinić, I.,& Lozo, J.. (2017). Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes. in Beneficial Microbes
Wageningen Academic Publishers, Wageningen., 8(5), 809-822.
https://doi.org/10.3920/BM2017.0007
Lukić J, Jancić I, Mirković N, Bufan B, Đokić J, Milenković M, Begović J, Strahinić I, Lozo J. Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes. in Beneficial Microbes. 2017;8(5):809-822.
doi:10.3920/BM2017.0007 .
Lukić, Jovanka, Jancić, I., Mirković, N., Bufan, B., Đokić, Jelena, Milenković, M., Begović, Jelena, Strahinić, Ivana, Lozo, Jelena, "Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes" in Beneficial Microbes, 8, no. 5 (2017):809-822,
https://doi.org/10.3920/BM2017.0007 . .
13
10
14

Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates

Milenković, Jelena; Hrenović, Jasna; Matijašević, Danka; Nikšić, Miomir; Rajić, Nevenka

(Springer Heidelberg, Heidelberg, 2017)

TY  - JOUR
AU  - Milenković, Jelena
AU  - Hrenović, Jasna
AU  - Matijašević, Danka
AU  - Nikšić, Miomir
AU  - Rajić, Nevenka
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1002
AB  - Two types of zeolites-natural clinoptilolite (NZ) and synthetic zeolite A (A)-were enriched with approx. 0.25 mmol of Cu(II), Zn(II), or Ag(I) ions, and the obtained materials (M-Z) were tested against three different isolates of Escherichia coli. Two isolates were environmental isolates from waters in Serbia whereas the third one was DSM 498. Antibacterial activity was studied in different water media-nutrient-rich media (peptone water), water from Sava Lake, and commercially available spring water. The Ag-containing zeolites showed bactericidal activity in the nutrient-rich peptone water after 1 h of contact. Cu- and Zn-containing zeolites showed bactericidal activity in real water samples. Antibacterial activity of the M-Z decreases in all three examined water media in the following order: Ag-NZ ae Ag-A  gt  Cu-NZ ae Cu-A  gt  Zn-NZ  gt  gt  gt  Zn-A, suggesting that mainly the metal type and not the zeolite type have a role in the antibacterial activity. Leaching experiments showed small amounts of the leached Cu(II) and Zn(II) ions, indicating that the antibacterial activity is not due to the metal ions but should be attributed to the M-Z itself. However, leached amounts of Ag(I) from Ag-NZ and Ag-A in peptone water indicate that the released Ag(I) could be mainly responsible for the bactericidal effect of the Ag(I)-containing zeolites. Since no loss of cellular material was found, the antibacterial activity is not attributed to cytoplasmic membrane damage.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates
EP  - 20281
IS  - 25
SP  - 20273
VL  - 24
DO  - 10.1007/s11356-017-9643-8
ER  - 
@article{
author = "Milenković, Jelena and Hrenović, Jasna and Matijašević, Danka and Nikšić, Miomir and Rajić, Nevenka",
year = "2017",
abstract = "Two types of zeolites-natural clinoptilolite (NZ) and synthetic zeolite A (A)-were enriched with approx. 0.25 mmol of Cu(II), Zn(II), or Ag(I) ions, and the obtained materials (M-Z) were tested against three different isolates of Escherichia coli. Two isolates were environmental isolates from waters in Serbia whereas the third one was DSM 498. Antibacterial activity was studied in different water media-nutrient-rich media (peptone water), water from Sava Lake, and commercially available spring water. The Ag-containing zeolites showed bactericidal activity in the nutrient-rich peptone water after 1 h of contact. Cu- and Zn-containing zeolites showed bactericidal activity in real water samples. Antibacterial activity of the M-Z decreases in all three examined water media in the following order: Ag-NZ ae Ag-A  gt  Cu-NZ ae Cu-A  gt  Zn-NZ  gt  gt  gt  Zn-A, suggesting that mainly the metal type and not the zeolite type have a role in the antibacterial activity. Leaching experiments showed small amounts of the leached Cu(II) and Zn(II) ions, indicating that the antibacterial activity is not due to the metal ions but should be attributed to the M-Z itself. However, leached amounts of Ag(I) from Ag-NZ and Ag-A in peptone water indicate that the released Ag(I) could be mainly responsible for the bactericidal effect of the Ag(I)-containing zeolites. Since no loss of cellular material was found, the antibacterial activity is not attributed to cytoplasmic membrane damage.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates",
pages = "20281-20273",
number = "25",
volume = "24",
doi = "10.1007/s11356-017-9643-8"
}
Milenković, J., Hrenović, J., Matijašević, D., Nikšić, M.,& Rajić, N.. (2017). Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 24(25), 20273-20281.
https://doi.org/10.1007/s11356-017-9643-8
Milenković J, Hrenović J, Matijašević D, Nikšić M, Rajić N. Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates. in Environmental Science and Pollution Research. 2017;24(25):20273-20281.
doi:10.1007/s11356-017-9643-8 .
Milenković, Jelena, Hrenović, Jasna, Matijašević, Danka, Nikšić, Miomir, Rajić, Nevenka, "Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates" in Environmental Science and Pollution Research, 24, no. 25 (2017):20273-20281,
https://doi.org/10.1007/s11356-017-9643-8 . .
60
30
60

The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis

Matijašević, Danka; Pantić, Milena; Rasković, Bozidar; Pavlović, Vladimir; Duvnjak, Dunja; Sknepnek, Aleksandra; Nikšić, Miomir

(Frontiers Media Sa, Lausanne, 2016)

TY  - JOUR
AU  - Matijašević, Danka
AU  - Pantić, Milena
AU  - Rasković, Bozidar
AU  - Pavlović, Vladimir
AU  - Duvnjak, Dunja
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/916
AB  - The antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete Coriolus versicolor was examined. The Minimum Inhibitory Concentration (MIC) values against various bacteria ranged from 0.625 to 20 mg mL(-1). versicolor expressed bactericidal activity against both Gram-positive and Gram-negative bacteria. The growth curves of Staphylococcus aureus and Salmonella enterica serovar Enteritidis, measured at 630 nm, and confirmed with macrodilution method showed that the obtained extract could inhibit the growth of tested bacteria. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the loss of 260-nm-absorbing material were used to examine the ultrastructural changes in bacteria induced by the extract. When S. aureus was exposed to the MIC of C. versicolor, elongated and malformed cells were observed by SEM, while S. Enteritidis treated cells appeared shorter and aggregated with ruptured cell walls. TEM revealed the formation of non-membrane-enclosed bodies and depleted inner content of S. aureus. Larger and irregular periplasmic space and deformed and scattered components of the cell envelope were observed in treated S. Enteritidis. The loss of 260-nm-absorbing material indicated that the disruptive action of the extract on cytoplasmic membrane was more pronounced in S. aureus than in S. Enteritidis treated cells. The UV and FTIR spectrophotometric analyses revealed diverse composition of C. versicolor extract and high content of total phenolics. Altogether, mushroom extracts could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis
VL  - 7
DO  - 10.3389/fmicb.2016.01226
ER  - 
@article{
author = "Matijašević, Danka and Pantić, Milena and Rasković, Bozidar and Pavlović, Vladimir and Duvnjak, Dunja and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2016",
abstract = "The antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete Coriolus versicolor was examined. The Minimum Inhibitory Concentration (MIC) values against various bacteria ranged from 0.625 to 20 mg mL(-1). versicolor expressed bactericidal activity against both Gram-positive and Gram-negative bacteria. The growth curves of Staphylococcus aureus and Salmonella enterica serovar Enteritidis, measured at 630 nm, and confirmed with macrodilution method showed that the obtained extract could inhibit the growth of tested bacteria. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the loss of 260-nm-absorbing material were used to examine the ultrastructural changes in bacteria induced by the extract. When S. aureus was exposed to the MIC of C. versicolor, elongated and malformed cells were observed by SEM, while S. Enteritidis treated cells appeared shorter and aggregated with ruptured cell walls. TEM revealed the formation of non-membrane-enclosed bodies and depleted inner content of S. aureus. Larger and irregular periplasmic space and deformed and scattered components of the cell envelope were observed in treated S. Enteritidis. The loss of 260-nm-absorbing material indicated that the disruptive action of the extract on cytoplasmic membrane was more pronounced in S. aureus than in S. Enteritidis treated cells. The UV and FTIR spectrophotometric analyses revealed diverse composition of C. versicolor extract and high content of total phenolics. Altogether, mushroom extracts could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis",
volume = "7",
doi = "10.3389/fmicb.2016.01226"
}
Matijašević, D., Pantić, M., Rasković, B., Pavlović, V., Duvnjak, D., Sknepnek, A.,& Nikšić, M.. (2016). The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmicb.2016.01226
Matijašević D, Pantić M, Rasković B, Pavlović V, Duvnjak D, Sknepnek A, Nikšić M. The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis. in Frontiers in Microbiology. 2016;7.
doi:10.3389/fmicb.2016.01226 .
Matijašević, Danka, Pantić, Milena, Rasković, Bozidar, Pavlović, Vladimir, Duvnjak, Dunja, Sknepnek, Aleksandra, Nikšić, Miomir, "The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis" in Frontiers in Microbiology, 7 (2016),
https://doi.org/10.3389/fmicb.2016.01226 . .
1
76
39
76

Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds

Duvnjak, Dunja; Pantić, Milena; Pavlović, Vladimir; Nedović, Viktor; Lević, Steva; Matijašević, Danka; Sknepnek, Aleksandra; Nikšić, Miomir

(Elsevier Sci Ltd, Oxford, 2016)

TY  - JOUR
AU  - Duvnjak, Dunja
AU  - Pantić, Milena
AU  - Pavlović, Vladimir
AU  - Nedović, Viktor
AU  - Lević, Steva
AU  - Matijašević, Danka
AU  - Sknepnek, Aleksandra
AU  - Nikšić, Miomir
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/969
AB  - Bioactive compounds obtained from Coriolus versicolor (Trametes versicolor (L: Fr) Lloyd, 1920.) mushrooms cultivated in a stirred-tank bioreactor were tested to determine their antimicrobial potential. Extracellular polysaccharides were isolated from the fermentation broth by ethanol precipitation. A methanol extract was prepared from mycelium. The cultivation conditions applied during the fermentation process provided for significant biomass 6.63 +/- 0.31 g dry weight L-1 and yield of extracellular polysaccharides (EPS) (0.74 +/- 0.12 g L-1). Microscopic analyses revealed that the mycelium grew predominately in the form of fluffy pellets. The methanol extract demonstrated very good activity against all the tested Gram-positive bacteria. Bacillus spizizeni and Staphylococcus epidermidis were the most sensitive strains (minimum inhibitory concentration (MIC)  lt 0.3125 mg mL(-1)). Among the Gram-negative bacteria, Yersinia enterocolitica had the lowest MIC value, 5 mg mL(-1). Microbicidal activity of mycelia methanol extract was established in seven out of ten tested Gram-negative bacteria strains with minimum bactericidal concentration (MBC) values ranged from 20 to 40 mg mL(-1). Enterococcus faecalis and Staphylococcus aureus showed higher sensitivity to the extracellular polysaccharides (MIC values 2.5 mg mL(-1)). FTIR analysis revealed a more complex chemical composition of the methanol extract compared to EPS, which might explain the better antibacterial activity of the methanol extract. Our results suggested that the submerged cultivation of Coriolus versicolor followed by ethanol precipitation of EPS and the methanol extraction of mycelia can be a promising process to obtain biological active compounds with significant antimicrobial activity. Industrial Relevance: Mushrooms contain a large number of chemicals with potential use as antimicrobial compounds. One of the biggest challenges for providing biologically active compounds from mushrooms is short-term process standardization with a low risk for contamination. Submerged culture cultivation is the best choice for providing antimicrobial compounds from mushrooms. The submerged culture method represents an effective and energy-efficient means to produce novel antibacterial compound from mushrooms. Antibacterial activity testing revealed that methanol extract and isolated exopolysaccharides exhibited strong antibacterial activity, especially against Gram-positive bacteria.
PB  - Elsevier Sci Ltd, Oxford
T2  - Innovative Food Science & Emerging Technologies
T1  - Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds
EP  - 8
SP  - 1
VL  - 34
DO  - 10.1016/j.ifset.2015.12.028
ER  - 
@article{
author = "Duvnjak, Dunja and Pantić, Milena and Pavlović, Vladimir and Nedović, Viktor and Lević, Steva and Matijašević, Danka and Sknepnek, Aleksandra and Nikšić, Miomir",
year = "2016",
abstract = "Bioactive compounds obtained from Coriolus versicolor (Trametes versicolor (L: Fr) Lloyd, 1920.) mushrooms cultivated in a stirred-tank bioreactor were tested to determine their antimicrobial potential. Extracellular polysaccharides were isolated from the fermentation broth by ethanol precipitation. A methanol extract was prepared from mycelium. The cultivation conditions applied during the fermentation process provided for significant biomass 6.63 +/- 0.31 g dry weight L-1 and yield of extracellular polysaccharides (EPS) (0.74 +/- 0.12 g L-1). Microscopic analyses revealed that the mycelium grew predominately in the form of fluffy pellets. The methanol extract demonstrated very good activity against all the tested Gram-positive bacteria. Bacillus spizizeni and Staphylococcus epidermidis were the most sensitive strains (minimum inhibitory concentration (MIC)  lt 0.3125 mg mL(-1)). Among the Gram-negative bacteria, Yersinia enterocolitica had the lowest MIC value, 5 mg mL(-1). Microbicidal activity of mycelia methanol extract was established in seven out of ten tested Gram-negative bacteria strains with minimum bactericidal concentration (MBC) values ranged from 20 to 40 mg mL(-1). Enterococcus faecalis and Staphylococcus aureus showed higher sensitivity to the extracellular polysaccharides (MIC values 2.5 mg mL(-1)). FTIR analysis revealed a more complex chemical composition of the methanol extract compared to EPS, which might explain the better antibacterial activity of the methanol extract. Our results suggested that the submerged cultivation of Coriolus versicolor followed by ethanol precipitation of EPS and the methanol extraction of mycelia can be a promising process to obtain biological active compounds with significant antimicrobial activity. Industrial Relevance: Mushrooms contain a large number of chemicals with potential use as antimicrobial compounds. One of the biggest challenges for providing biologically active compounds from mushrooms is short-term process standardization with a low risk for contamination. Submerged culture cultivation is the best choice for providing antimicrobial compounds from mushrooms. The submerged culture method represents an effective and energy-efficient means to produce novel antibacterial compound from mushrooms. Antibacterial activity testing revealed that methanol extract and isolated exopolysaccharides exhibited strong antibacterial activity, especially against Gram-positive bacteria.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Innovative Food Science & Emerging Technologies",
title = "Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds",
pages = "8-1",
volume = "34",
doi = "10.1016/j.ifset.2015.12.028"
}
Duvnjak, D., Pantić, M., Pavlović, V., Nedović, V., Lević, S., Matijašević, D., Sknepnek, A.,& Nikšić, M.. (2016). Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds. in Innovative Food Science & Emerging Technologies
Elsevier Sci Ltd, Oxford., 34, 1-8.
https://doi.org/10.1016/j.ifset.2015.12.028
Duvnjak D, Pantić M, Pavlović V, Nedović V, Lević S, Matijašević D, Sknepnek A, Nikšić M. Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds. in Innovative Food Science & Emerging Technologies. 2016;34:1-8.
doi:10.1016/j.ifset.2015.12.028 .
Duvnjak, Dunja, Pantić, Milena, Pavlović, Vladimir, Nedović, Viktor, Lević, Steva, Matijašević, Danka, Sknepnek, Aleksandra, Nikšić, Miomir, "Advances in in batch culture fermented Coriolus versicolor medicinal mushroom for the production of antibacterial compounds" in Innovative Food Science & Emerging Technologies, 34 (2016):1-8,
https://doi.org/10.1016/j.ifset.2015.12.028 . .
31
24
36

Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain

Mirković, Nemanja; Radulović, Zorica; Uzelac, Gordana; Lozo, Jelena; Obradović, Dragojlo; Topisirović, Ljubiša; Kojić, Milan

(University of Zagreb, 2015)

TY  - JOUR
AU  - Mirković, Nemanja
AU  - Radulović, Zorica
AU  - Uzelac, Gordana
AU  - Lozo, Jelena
AU  - Obradović, Dragojlo
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/870
AB  - Lactococcus locus ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Zanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of different sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specific for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain
EP  - 242
IS  - 2
SP  - 237
VL  - 53
DO  - 10.17113/ftb.53.02.15.3846
ER  - 
@article{
author = "Mirković, Nemanja and Radulović, Zorica and Uzelac, Gordana and Lozo, Jelena and Obradović, Dragojlo and Topisirović, Ljubiša and Kojić, Milan",
year = "2015",
abstract = "Lactococcus locus ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Zanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of different sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specific for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain",
pages = "242-237",
number = "2",
volume = "53",
doi = "10.17113/ftb.53.02.15.3846"
}
Mirković, N., Radulović, Z., Uzelac, G., Lozo, J., Obradović, D., Topisirović, L.,& Kojić, M.. (2015). Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain. in Food Technology and Biotechnology
University of Zagreb., 53(2), 237-242.
https://doi.org/10.17113/ftb.53.02.15.3846
Mirković N, Radulović Z, Uzelac G, Lozo J, Obradović D, Topisirović L, Kojić M. Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain. in Food Technology and Biotechnology. 2015;53(2):237-242.
doi:10.17113/ftb.53.02.15.3846 .
Mirković, Nemanja, Radulović, Zorica, Uzelac, Gordana, Lozo, Jelena, Obradović, Dragojlo, Topisirović, Ljubiša, Kojić, Milan, "Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain" in Food Technology and Biotechnology, 53, no. 2 (2015):237-242,
https://doi.org/10.17113/ftb.53.02.15.3846 . .
7
5
7

The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms agaricus bisporus and pleurotus spp.

Savić, Milena; Anđelković, I.; Duvnjak, Dunja; Matijašević, Danka; Avramović, Aleksandra; Nikšić, Miomir

(Srpsko biološko društvo, Beograd, i dr., 2012)

TY  - JOUR
AU  - Savić, Milena
AU  - Anđelković, I.
AU  - Duvnjak, Dunja
AU  - Matijašević, Danka
AU  - Avramović, Aleksandra
AU  - Nikšić, Miomir
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/607
AB  - The activity of organic selenium against pathogenic molds and its use as a potential selenium source in the production of enriched mushrooms were examined. The effect of commercial selenized yeast on mycelia growth was examined using a method with mycelia disks and a well diffusion method. For mushroom enrichment, different concentrations of selenium were added to a growth substrate. The results presented in this paper suggest that the most suitable concentration of selenized yeast that inhibits the growth of the mycopathogenic molds is 70-100 mg/kg of selenium. With the addition of this concentration to the substrate, mushroom fruit bodies will uptake a high level of selenium, about 100 mu g/g for Pleurotus spp., and 200 mu/g for Agaricus bisporus in dry weight of the mushroom. Thereby a double effect in the cultivation of mushrooms is achieved.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms agaricus bisporus and pleurotus spp.
EP  - 1463
IS  - 4
SP  - 1455
VL  - 64
DO  - 10.2298/ABS1204455S
ER  - 
@article{
author = "Savić, Milena and Anđelković, I. and Duvnjak, Dunja and Matijašević, Danka and Avramović, Aleksandra and Nikšić, Miomir",
year = "2012",
abstract = "The activity of organic selenium against pathogenic molds and its use as a potential selenium source in the production of enriched mushrooms were examined. The effect of commercial selenized yeast on mycelia growth was examined using a method with mycelia disks and a well diffusion method. For mushroom enrichment, different concentrations of selenium were added to a growth substrate. The results presented in this paper suggest that the most suitable concentration of selenized yeast that inhibits the growth of the mycopathogenic molds is 70-100 mg/kg of selenium. With the addition of this concentration to the substrate, mushroom fruit bodies will uptake a high level of selenium, about 100 mu g/g for Pleurotus spp., and 200 mu/g for Agaricus bisporus in dry weight of the mushroom. Thereby a double effect in the cultivation of mushrooms is achieved.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms agaricus bisporus and pleurotus spp.",
pages = "1463-1455",
number = "4",
volume = "64",
doi = "10.2298/ABS1204455S"
}
Savić, M., Anđelković, I., Duvnjak, D., Matijašević, D., Avramović, A.,& Nikšić, M.. (2012). The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms agaricus bisporus and pleurotus spp.. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1455-1463.
https://doi.org/10.2298/ABS1204455S
Savić M, Anđelković I, Duvnjak D, Matijašević D, Avramović A, Nikšić M. The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms agaricus bisporus and pleurotus spp.. in Archives of Biological Sciences. 2012;64(4):1455-1463.
doi:10.2298/ABS1204455S .
Savić, Milena, Anđelković, I., Duvnjak, Dunja, Matijašević, Danka, Avramović, Aleksandra, Nikšić, Miomir, "The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms agaricus bisporus and pleurotus spp." in Archives of Biological Sciences, 64, no. 4 (2012):1455-1463,
https://doi.org/10.2298/ABS1204455S . .
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