TY  - JOUR
AU  - Jovanović, Marina
AU  - Morić, Ivana
AU  - Nikolić, Biljana
AU  - Pavić, Aleksandar
AU  - Svircev, Emilija
AU  - Šenerović, Lidija
AU  - Mitić-Culafić, Dragana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1320
AB  - Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections.
PB  - MDPI, Basel
T2  - Molecules
T1  - Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts
IS  - 8
VL  - 25
DO  - 10.3390/molecules25081811
ER  - 

@article{
author = "Jovanović, Marina and Morić, Ivana and Nikolić, Biljana and Pavić, Aleksandar and Svircev, Emilija and Šenerović, Lidija and Mitić-Culafić, Dragana",
year = "2020",
abstract = "Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts",
number = "8",
volume = "25",
doi = "10.3390/molecules25081811"
}

Jovanović, M., Morić, I., Nikolić, B., Pavić, A., Svircev, E., Šenerović, L.,& Mitić-Culafić, D.. (2020). Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts. in Molecules
MDPI, Basel., 25(8).
https://doi.org/10.3390/molecules25081811

Jovanović M, Morić I, Nikolić B, Pavić A, Svircev E, Šenerović L, Mitić-Culafić D. Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts. in Molecules. 2020;25(8).
doi:10.3390/molecules25081811 .

Jovanović, Marina, Morić, Ivana, Nikolić, Biljana, Pavić, Aleksandar, Svircev, Emilija, Šenerović, Lidija, Mitić-Culafić, Dragana, "Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts" in Molecules, 25, no. 8 (2020),
https://doi.org/10.3390/molecules25081811 . .

TY  - JOUR
AU  - Pavić, Aleksandar
AU  - Mitić-Culafić, Dragana
AU  - Nebojša, Jasnic
AU  - Nikolić, Biljana
AU  - Simin, Nataša
AU  - Vasiljević, Branka
AU  - Knežević-Vukčević, Jelena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1240
AB  - The objective of this study was to evaluate potential of two chemically characterized edible wild onion species, Allium flavum and Allium carinatum, to reduce side effects of cytostatic doxorubicin (Dox). Since Dox application is mainly limited due to its high cardiotoxicity, while there are no approved cardioprotective agents for the prevention of Dox adverse effects, new co-treatments are urgently needed. Here, we showed that methanol extracts expressed high antioxidant activity and synergistically increased Dox anticancer activity against human hepatoma (HepG2) and lung carcinoma (A549) cells, while protected normal human fibroblasts (MRC-5) from Dox cytotoxicity. Analysis of the antioxidative enzymes level (catalase and superoxide dismutases) showed that the catalase level was differently altered in cancer cells compared to normal cells upon applied treatments. In vivo toxicity evaluation in the zebrafish model revealed significantly lower toxicity of extracts compared to Dox, and no teratogenic effects at applied doses. We found that extracts successfully rescued the Dox-treated embryos of life-threating cardiomyopathy, while at the same time reduced developmental toxicity and neutropenia. Further analysis demonstrated that extracts had higher anti-angiogenic activity than sunitinib or auranofin, clinically used anti-angiogenic drugs. In addition, angiogenesis was markedly more suppressed in Dox-extract cotreatments than upon single treatments.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Biomedicine & Pharmacotherapy
T1  - Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin
EP  - 2491
SP  - 2482
VL  - 109
DO  - 10.1016/j.biopha.2018.11.106
ER  - 

@article{
author = "Pavić, Aleksandar and Mitić-Culafić, Dragana and Nebojša, Jasnic and Nikolić, Biljana and Simin, Nataša and Vasiljević, Branka and Knežević-Vukčević, Jelena",
year = "2019",
abstract = "The objective of this study was to evaluate potential of two chemically characterized edible wild onion species, Allium flavum and Allium carinatum, to reduce side effects of cytostatic doxorubicin (Dox). Since Dox application is mainly limited due to its high cardiotoxicity, while there are no approved cardioprotective agents for the prevention of Dox adverse effects, new co-treatments are urgently needed. Here, we showed that methanol extracts expressed high antioxidant activity and synergistically increased Dox anticancer activity against human hepatoma (HepG2) and lung carcinoma (A549) cells, while protected normal human fibroblasts (MRC-5) from Dox cytotoxicity. Analysis of the antioxidative enzymes level (catalase and superoxide dismutases) showed that the catalase level was differently altered in cancer cells compared to normal cells upon applied treatments. In vivo toxicity evaluation in the zebrafish model revealed significantly lower toxicity of extracts compared to Dox, and no teratogenic effects at applied doses. We found that extracts successfully rescued the Dox-treated embryos of life-threating cardiomyopathy, while at the same time reduced developmental toxicity and neutropenia. Further analysis demonstrated that extracts had higher anti-angiogenic activity than sunitinib or auranofin, clinically used anti-angiogenic drugs. In addition, angiogenesis was markedly more suppressed in Dox-extract cotreatments than upon single treatments.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Biomedicine & Pharmacotherapy",
title = "Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin",
pages = "2491-2482",
volume = "109",
doi = "10.1016/j.biopha.2018.11.106"
}

Pavić, A., Mitić-Culafić, D., Nebojša, J., Nikolić, B., Simin, N., Vasiljević, B.,& Knežević-Vukčević, J.. (2019). Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin. in Biomedicine & Pharmacotherapy
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 109, 2482-2491.
https://doi.org/10.1016/j.biopha.2018.11.106

Pavić A, Mitić-Culafić D, Nebojša J, Nikolić B, Simin N, Vasiljević B, Knežević-Vukčević J. Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin. in Biomedicine & Pharmacotherapy. 2019;109:2482-2491.
doi:10.1016/j.biopha.2018.11.106 .

Pavić, Aleksandar, Mitić-Culafić, Dragana, Nebojša, Jasnic, Nikolić, Biljana, Simin, Nataša, Vasiljević, Branka, Knežević-Vukčević, Jelena, "Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin" in Biomedicine & Pharmacotherapy, 109 (2019):2482-2491,
https://doi.org/10.1016/j.biopha.2018.11.106 . .