Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines
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2017
Authors
Ljujić, MilaMijatović, Sanja
Bulatović, Mirna Z.
Mojić, Marija
Maksimović-Ivanić, Danijela
Radojković, Dragica
Topić, Aleksandra
Article (Published version)
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Show full item recordAbstract
Increased circulating alpha-1-antitrypsin (AAT) correlates with cancer stage/aggressiveness, but its role in cancer biology is unclear. We revealed antagonistic effect of AAT to cisplatin-induced cytotoxicity in prostate (PC3) and melanoma (A375) cancer cell lines. Moreover, AAT abrogated cytotoxicity of MEK inhibitor U0126 in PC3 cell line. Weaker antagonistic effect of AAT on cytotoxicity of PI3/Akt and NF-kB inhibitors was also observed. In addition, cisplatin increased AAT gene expression in transfected PC3 cells. However, AAT derived from transfected PC3 cells did not antagonize cisplatin-induced cytotoxicity. In conclusion, these results suggest possible association between high circulating AAT and cisplatin resistance.
Keywords:
Prostate cancer / Melanoma cancer / Cisplatin / Alpha-1-antitrypsinSource:
Pathology & Oncology Research, 2017, 23, 2, 335-343Publisher:
- Springer, Dordrecht
Funding / projects:
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
- Complex diseases as a model system for phenotype modulation- structural and functional analysis of molecular biomarkers (RS-MESTD-Basic Research (BR or ON)-173008)
DOI: 10.1007/s12253-016-0104-3
ISSN: 1219-4956
PubMed: 27617337
WoS: 000399000200014
Scopus: 2-s2.0-85016626907
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Ljujić, Mila AU - Mijatović, Sanja AU - Bulatović, Mirna Z. AU - Mojić, Marija AU - Maksimović-Ivanić, Danijela AU - Radojković, Dragica AU - Topić, Aleksandra PY - 2017 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1044 AB - Increased circulating alpha-1-antitrypsin (AAT) correlates with cancer stage/aggressiveness, but its role in cancer biology is unclear. We revealed antagonistic effect of AAT to cisplatin-induced cytotoxicity in prostate (PC3) and melanoma (A375) cancer cell lines. Moreover, AAT abrogated cytotoxicity of MEK inhibitor U0126 in PC3 cell line. Weaker antagonistic effect of AAT on cytotoxicity of PI3/Akt and NF-kB inhibitors was also observed. In addition, cisplatin increased AAT gene expression in transfected PC3 cells. However, AAT derived from transfected PC3 cells did not antagonize cisplatin-induced cytotoxicity. In conclusion, these results suggest possible association between high circulating AAT and cisplatin resistance. PB - Springer, Dordrecht T2 - Pathology & Oncology Research T1 - Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines EP - 343 IS - 2 SP - 335 VL - 23 DO - 10.1007/s12253-016-0104-3 ER -
@article{ author = "Ljujić, Mila and Mijatović, Sanja and Bulatović, Mirna Z. and Mojić, Marija and Maksimović-Ivanić, Danijela and Radojković, Dragica and Topić, Aleksandra", year = "2017", abstract = "Increased circulating alpha-1-antitrypsin (AAT) correlates with cancer stage/aggressiveness, but its role in cancer biology is unclear. We revealed antagonistic effect of AAT to cisplatin-induced cytotoxicity in prostate (PC3) and melanoma (A375) cancer cell lines. Moreover, AAT abrogated cytotoxicity of MEK inhibitor U0126 in PC3 cell line. Weaker antagonistic effect of AAT on cytotoxicity of PI3/Akt and NF-kB inhibitors was also observed. In addition, cisplatin increased AAT gene expression in transfected PC3 cells. However, AAT derived from transfected PC3 cells did not antagonize cisplatin-induced cytotoxicity. In conclusion, these results suggest possible association between high circulating AAT and cisplatin resistance.", publisher = "Springer, Dordrecht", journal = "Pathology & Oncology Research", title = "Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines", pages = "343-335", number = "2", volume = "23", doi = "10.1007/s12253-016-0104-3" }
Ljujić, M., Mijatović, S., Bulatović, M. Z., Mojić, M., Maksimović-Ivanić, D., Radojković, D.,& Topić, A.. (2017). Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines. in Pathology & Oncology Research Springer, Dordrecht., 23(2), 335-343. https://doi.org/10.1007/s12253-016-0104-3
Ljujić M, Mijatović S, Bulatović MZ, Mojić M, Maksimović-Ivanić D, Radojković D, Topić A. Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines. in Pathology & Oncology Research. 2017;23(2):335-343. doi:10.1007/s12253-016-0104-3 .
Ljujić, Mila, Mijatović, Sanja, Bulatović, Mirna Z., Mojić, Marija, Maksimović-Ivanić, Danijela, Radojković, Dragica, Topić, Aleksandra, "Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines" in Pathology & Oncology Research, 23, no. 2 (2017):335-343, https://doi.org/10.1007/s12253-016-0104-3 . .