Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10
2018
Authors
Schneider, OlhaIlić-Tomić, Tatjana
Rueckert, Christian
Kalinowski, Joern
Gencić, Marija S.
Zivković, Milena Z.
Stanković, Nada
Radulović, Niko S.
Vasiljević, Branka
Nikodinović-Runić, Jasmina
Zotchev, Sergey B.
Article (Published version)
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Show full item recordAbstract
Streptomyces sp. NP10 was previously shown to synthesize large amounts of free fatty acids (FFAs). In this work, we report the first insights into the biosynthesis of these fatty acids (FAs) gained after genome sequencing and identification of the genes involved. Analysis of the Streptomyces sp. NP10 draft genome revealed that it is closely related to several strains of Streptomyces griseus. Comparative analyses of secondary metabolite biosynthetic gene clusters, as well as those presumably involved in FA biosynthesis, allowed identification of an unusual cluster C12-2, which could be identified in only one other S. griseus-related streptomycete. To prove the involvement of identified cluster in FFA biosynthesis, one of its three ketosynthase genes was insertionally inactivated to generate mutant strain mNP10. Accumulation of FFAs in mNP10 was almost completely abolished, reaching less than 0.01% compared to the wild-type strain. Cloning and transfer of the C12-2 cluster to the mNP10 m...utant partially restored FFA production, albeit to a low level. The discovery of this rare FFA biosynthesis cluster opens possibilities for detailed characterization of the roles of individual genes and their products in the biosynthesis of FFAs in NP10.
Keywords:
Streptomyces / insertional inactivation / genome sequence / fatty acid biosynthesis / biosynthetic gene clustersSource:
Frontiers in Microbiology, 2018, 9Publisher:
- Frontiers Media Sa, Lausanne
Funding / projects:
- Norwegian University of Science and Technology
- University of Vienna
- Combinatorial libraries of heterogeneous catalysts, natural products, and their derivatives and analogues: the way to biologically active compounds (RS-MESTD-Basic Research (BR or ON)-172061)
- Microbial diversity study and characterization of beneficial environmental microorganisms (RS-MESTD-Basic Research (BR or ON)-173048)
DOI: 10.3389/fmicb.2018.01302
ISSN: 1664-302X
WoS: 000435685800001
Scopus: 2-s2.0-85048678465
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Schneider, Olha AU - Ilić-Tomić, Tatjana AU - Rueckert, Christian AU - Kalinowski, Joern AU - Gencić, Marija S. AU - Zivković, Milena Z. AU - Stanković, Nada AU - Radulović, Niko S. AU - Vasiljević, Branka AU - Nikodinović-Runić, Jasmina AU - Zotchev, Sergey B. PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1107 AB - Streptomyces sp. NP10 was previously shown to synthesize large amounts of free fatty acids (FFAs). In this work, we report the first insights into the biosynthesis of these fatty acids (FAs) gained after genome sequencing and identification of the genes involved. Analysis of the Streptomyces sp. NP10 draft genome revealed that it is closely related to several strains of Streptomyces griseus. Comparative analyses of secondary metabolite biosynthetic gene clusters, as well as those presumably involved in FA biosynthesis, allowed identification of an unusual cluster C12-2, which could be identified in only one other S. griseus-related streptomycete. To prove the involvement of identified cluster in FFA biosynthesis, one of its three ketosynthase genes was insertionally inactivated to generate mutant strain mNP10. Accumulation of FFAs in mNP10 was almost completely abolished, reaching less than 0.01% compared to the wild-type strain. Cloning and transfer of the C12-2 cluster to the mNP10 mutant partially restored FFA production, albeit to a low level. The discovery of this rare FFA biosynthesis cluster opens possibilities for detailed characterization of the roles of individual genes and their products in the biosynthesis of FFAs in NP10. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Microbiology T1 - Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10 VL - 9 DO - 10.3389/fmicb.2018.01302 ER -
@article{ author = "Schneider, Olha and Ilić-Tomić, Tatjana and Rueckert, Christian and Kalinowski, Joern and Gencić, Marija S. and Zivković, Milena Z. and Stanković, Nada and Radulović, Niko S. and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Zotchev, Sergey B.", year = "2018", abstract = "Streptomyces sp. NP10 was previously shown to synthesize large amounts of free fatty acids (FFAs). In this work, we report the first insights into the biosynthesis of these fatty acids (FAs) gained after genome sequencing and identification of the genes involved. Analysis of the Streptomyces sp. NP10 draft genome revealed that it is closely related to several strains of Streptomyces griseus. Comparative analyses of secondary metabolite biosynthetic gene clusters, as well as those presumably involved in FA biosynthesis, allowed identification of an unusual cluster C12-2, which could be identified in only one other S. griseus-related streptomycete. To prove the involvement of identified cluster in FFA biosynthesis, one of its three ketosynthase genes was insertionally inactivated to generate mutant strain mNP10. Accumulation of FFAs in mNP10 was almost completely abolished, reaching less than 0.01% compared to the wild-type strain. Cloning and transfer of the C12-2 cluster to the mNP10 mutant partially restored FFA production, albeit to a low level. The discovery of this rare FFA biosynthesis cluster opens possibilities for detailed characterization of the roles of individual genes and their products in the biosynthesis of FFAs in NP10.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Microbiology", title = "Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10", volume = "9", doi = "10.3389/fmicb.2018.01302" }
Schneider, O., Ilić-Tomić, T., Rueckert, C., Kalinowski, J., Gencić, M. S., Zivković, M. Z., Stanković, N., Radulović, N. S., Vasiljević, B., Nikodinović-Runić, J.,& Zotchev, S. B.. (2018). Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10. in Frontiers in Microbiology Frontiers Media Sa, Lausanne., 9. https://doi.org/10.3389/fmicb.2018.01302
Schneider O, Ilić-Tomić T, Rueckert C, Kalinowski J, Gencić MS, Zivković MZ, Stanković N, Radulović NS, Vasiljević B, Nikodinović-Runić J, Zotchev SB. Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10. in Frontiers in Microbiology. 2018;9. doi:10.3389/fmicb.2018.01302 .
Schneider, Olha, Ilić-Tomić, Tatjana, Rueckert, Christian, Kalinowski, Joern, Gencić, Marija S., Zivković, Milena Z., Stanković, Nada, Radulović, Niko S., Vasiljević, Branka, Nikodinović-Runić, Jasmina, Zotchev, Sergey B., "Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp NP10" in Frontiers in Microbiology, 9 (2018), https://doi.org/10.3389/fmicb.2018.01302 . .