New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates
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2018
Autori
Popović, Nikola![](/themes/Mirageimagine/images/orcid.png)
Dinić, Miroslav
![](/themes/Mirageimagine/images/orcid.png)
Tolinački, Maja
![](/themes/Mirageimagine/images/orcid.png)
Mihajlović, Sanja
Terzić-Vidojević, Amarela
![](/themes/Mirageimagine/images/orcid.png)
Bojić, Svetlana
Đokić, Jelena
![](/themes/Mirageimagine/images/orcid.png)
Golić, Nataša
![](/themes/Mirageimagine/images/orcid.png)
Veljović, Katarina
![](/themes/Mirageimagine/images/orcid.png)
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Enterococci have controversial status due to their emerging role in nosocomial infections and transmission of antibiotic resistance genes, while some enterococci strains are used as probiotics for humans and animals and starter cultures in dairy industry. In order to improve our understanding of factors involved in the safe use of enterococci as potential probiotics, the antibiotic susceptibility, virulence and probiotic traits of 75 dairy enterococci isolates belonging to Enterococcus durans (50), En. faeciurn (15), En. faecalis (6), En. italious (3), and En, hirae (1) were evaluated. The results revealed that ciprofloxacin resistance and biofilm formation are correlated with isolates originated from Golija mountain (Serbia), while gelatinase activity was more common in isolates from Prigorje region (Croatia), pointing to uncontrolled use of antibiotics and anthropogenic impact on dairy products" microbiota in these regions. The virulence genes were sporadically present in 13 selected... dairy enterococci isolates. Interestingly, biofilm formation was correlated with higher ability of strains to reduce the adhesion of E. coil and Salmonella Enteritidis to HT29-MTX cells. To our knowledge this is the first study reporting the presence of the esp gene (previously correlated with pathogenesis) in dairy enterococci isolates, mostly associated with the genes involved in adhesion property. Hence, the results of this study revealed that the virulence genes are sporadically present in dairy isolates and more correlated to adhesion properties and biofilm formation, implicating their role in gut colonization rather than to the virulence traits.
Ključne reči:
virulence / pathogen exclusion / Enterococcus sp. / dairy isolates / biofilm formation / antibiotic resistance / adhesionIzvor:
Frontiers in Microbiology, 2018, 9Izdavač:
- Frontiers Media Sa, Lausanne
Finansiranje / projekti:
- Izučavanje gena i molekularnih mehanizama u osnovi probiotičke aktivnosti bakterija mlečne kiseline izolovanih sa područja zapadnog Balkana (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.3389/fmicb.2018.00078
ISSN: 1664-302X
WoS: 000423544100003
Scopus: 2-s2.0-85041631915
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Popović, Nikola AU - Dinić, Miroslav AU - Tolinački, Maja AU - Mihajlović, Sanja AU - Terzić-Vidojević, Amarela AU - Bojić, Svetlana AU - Đokić, Jelena AU - Golić, Nataša AU - Veljović, Katarina PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1157 AB - Enterococci have controversial status due to their emerging role in nosocomial infections and transmission of antibiotic resistance genes, while some enterococci strains are used as probiotics for humans and animals and starter cultures in dairy industry. In order to improve our understanding of factors involved in the safe use of enterococci as potential probiotics, the antibiotic susceptibility, virulence and probiotic traits of 75 dairy enterococci isolates belonging to Enterococcus durans (50), En. faeciurn (15), En. faecalis (6), En. italious (3), and En, hirae (1) were evaluated. The results revealed that ciprofloxacin resistance and biofilm formation are correlated with isolates originated from Golija mountain (Serbia), while gelatinase activity was more common in isolates from Prigorje region (Croatia), pointing to uncontrolled use of antibiotics and anthropogenic impact on dairy products" microbiota in these regions. The virulence genes were sporadically present in 13 selected dairy enterococci isolates. Interestingly, biofilm formation was correlated with higher ability of strains to reduce the adhesion of E. coil and Salmonella Enteritidis to HT29-MTX cells. To our knowledge this is the first study reporting the presence of the esp gene (previously correlated with pathogenesis) in dairy enterococci isolates, mostly associated with the genes involved in adhesion property. Hence, the results of this study revealed that the virulence genes are sporadically present in dairy isolates and more correlated to adhesion properties and biofilm formation, implicating their role in gut colonization rather than to the virulence traits. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Microbiology T1 - New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates VL - 9 DO - 10.3389/fmicb.2018.00078 ER -
@article{ author = "Popović, Nikola and Dinić, Miroslav and Tolinački, Maja and Mihajlović, Sanja and Terzić-Vidojević, Amarela and Bojić, Svetlana and Đokić, Jelena and Golić, Nataša and Veljović, Katarina", year = "2018", abstract = "Enterococci have controversial status due to their emerging role in nosocomial infections and transmission of antibiotic resistance genes, while some enterococci strains are used as probiotics for humans and animals and starter cultures in dairy industry. In order to improve our understanding of factors involved in the safe use of enterococci as potential probiotics, the antibiotic susceptibility, virulence and probiotic traits of 75 dairy enterococci isolates belonging to Enterococcus durans (50), En. faeciurn (15), En. faecalis (6), En. italious (3), and En, hirae (1) were evaluated. The results revealed that ciprofloxacin resistance and biofilm formation are correlated with isolates originated from Golija mountain (Serbia), while gelatinase activity was more common in isolates from Prigorje region (Croatia), pointing to uncontrolled use of antibiotics and anthropogenic impact on dairy products" microbiota in these regions. The virulence genes were sporadically present in 13 selected dairy enterococci isolates. Interestingly, biofilm formation was correlated with higher ability of strains to reduce the adhesion of E. coil and Salmonella Enteritidis to HT29-MTX cells. To our knowledge this is the first study reporting the presence of the esp gene (previously correlated with pathogenesis) in dairy enterococci isolates, mostly associated with the genes involved in adhesion property. Hence, the results of this study revealed that the virulence genes are sporadically present in dairy isolates and more correlated to adhesion properties and biofilm formation, implicating their role in gut colonization rather than to the virulence traits.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Microbiology", title = "New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates", volume = "9", doi = "10.3389/fmicb.2018.00078" }
Popović, N., Dinić, M., Tolinački, M., Mihajlović, S., Terzić-Vidojević, A., Bojić, S., Đokić, J., Golić, N.,& Veljović, K.. (2018). New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates. in Frontiers in Microbiology Frontiers Media Sa, Lausanne., 9. https://doi.org/10.3389/fmicb.2018.00078
Popović N, Dinić M, Tolinački M, Mihajlović S, Terzić-Vidojević A, Bojić S, Đokić J, Golić N, Veljović K. New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates. in Frontiers in Microbiology. 2018;9. doi:10.3389/fmicb.2018.00078 .
Popović, Nikola, Dinić, Miroslav, Tolinački, Maja, Mihajlović, Sanja, Terzić-Vidojević, Amarela, Bojić, Svetlana, Đokić, Jelena, Golić, Nataša, Veljović, Katarina, "New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates" in Frontiers in Microbiology, 9 (2018), https://doi.org/10.3389/fmicb.2018.00078 . .