Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics
Abstract
Crown imperial (CI) has been used in traditional medicine. Today it is known that such beneficial effects are due to its richness in steroidal alkaloids (SA). Using de novo transcriptomics, orthologues/paralogues finder, phylogenetic analysis and tissue- and developmental stage-specific expression analysis, we identified ten genes and several TFs involved in the biosynthesis of SA in CI. The comparative analysis of ten genes expression profiles revealed the possibility of their co-regulation, which may imply the possibility of their organization in metabolic gene clusters. Having in mind convergent evolution of steroidal biosynthetic pathways in flowering plants and records of convergent evolution of specific proteins, observed expression patterns open a reasonable interest to investigate the possibility of the existence of genes cluster organization in SA pathway in the family Liliaceae or at least in some species of genus Fritillaria. Obtained results support transcriptomics as usefu...l approach in elucidating genes underlying complex biochemical pathways.
Keywords:
Steroidal alkaloids / Fritillaria imperialis / Expression profiles / de novo transcriptomicsSource:
Genomics, 2019, 111, 6, 1360-1372Publisher:
- Academic Press Inc Elsevier Science, San Diego
Funding / projects:
- Shahrekord University
- Biotechnology Research Institute at the University of Shahrekord
DOI: 10.1016/j.ygeno.2018.09.008
ISSN: 0888-7543
PubMed: 30248489
WoS: 000495876600022
Scopus: 2-s2.0-85053928856
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Eshaghi, Mahsa AU - Shiran, Behrouz AU - Fallahi, Hossein AU - Ravash, Rudabeh AU - Banović Đeri, Bojana PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1218 AB - Crown imperial (CI) has been used in traditional medicine. Today it is known that such beneficial effects are due to its richness in steroidal alkaloids (SA). Using de novo transcriptomics, orthologues/paralogues finder, phylogenetic analysis and tissue- and developmental stage-specific expression analysis, we identified ten genes and several TFs involved in the biosynthesis of SA in CI. The comparative analysis of ten genes expression profiles revealed the possibility of their co-regulation, which may imply the possibility of their organization in metabolic gene clusters. Having in mind convergent evolution of steroidal biosynthetic pathways in flowering plants and records of convergent evolution of specific proteins, observed expression patterns open a reasonable interest to investigate the possibility of the existence of genes cluster organization in SA pathway in the family Liliaceae or at least in some species of genus Fritillaria. Obtained results support transcriptomics as useful approach in elucidating genes underlying complex biochemical pathways. PB - Academic Press Inc Elsevier Science, San Diego T2 - Genomics T1 - Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics EP - 1372 IS - 6 SP - 1360 VL - 111 DO - 10.1016/j.ygeno.2018.09.008 ER -
@article{ author = "Eshaghi, Mahsa and Shiran, Behrouz and Fallahi, Hossein and Ravash, Rudabeh and Banović Đeri, Bojana", year = "2019", abstract = "Crown imperial (CI) has been used in traditional medicine. Today it is known that such beneficial effects are due to its richness in steroidal alkaloids (SA). Using de novo transcriptomics, orthologues/paralogues finder, phylogenetic analysis and tissue- and developmental stage-specific expression analysis, we identified ten genes and several TFs involved in the biosynthesis of SA in CI. The comparative analysis of ten genes expression profiles revealed the possibility of their co-regulation, which may imply the possibility of their organization in metabolic gene clusters. Having in mind convergent evolution of steroidal biosynthetic pathways in flowering plants and records of convergent evolution of specific proteins, observed expression patterns open a reasonable interest to investigate the possibility of the existence of genes cluster organization in SA pathway in the family Liliaceae or at least in some species of genus Fritillaria. Obtained results support transcriptomics as useful approach in elucidating genes underlying complex biochemical pathways.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Genomics", title = "Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics", pages = "1372-1360", number = "6", volume = "111", doi = "10.1016/j.ygeno.2018.09.008" }
Eshaghi, M., Shiran, B., Fallahi, H., Ravash, R.,& Banović Đeri, B.. (2019). Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics. in Genomics Academic Press Inc Elsevier Science, San Diego., 111(6), 1360-1372. https://doi.org/10.1016/j.ygeno.2018.09.008
Eshaghi M, Shiran B, Fallahi H, Ravash R, Banović Đeri B. Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics. in Genomics. 2019;111(6):1360-1372. doi:10.1016/j.ygeno.2018.09.008 .
Eshaghi, Mahsa, Shiran, Behrouz, Fallahi, Hossein, Ravash, Rudabeh, Banović Đeri, Bojana, "Identification of genes involved in steroid alkaloid biosynthesis in Fritillaria imperialis via de novo transcriptomics" in Genomics, 111, no. 6 (2019):1360-1372, https://doi.org/10.1016/j.ygeno.2018.09.008 . .