SOX3 can promote the malignant behavior of glioblastoma cells
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Autori
Vicentić, Jelena MarjanovicDrakulić, Danijela
Garcia, Idoia
Vuković, Vladanka
Aldaz, Paula
Puskas, Nela
Nikolić, Igor
Tasić, Goran
Raicević, Savo
Garros-Regulez, Laura
Sampron, Nicolas
Atkinson, Michael J.
Anastasov, Nataša
Matheu, Ander
Stevanović, Milena
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
PurposeGlioblastoma is the most common and lethal adult brain tumor. Despite current therapeutic strategies, including surgery, radiation and chemotherapy, the median survival of glioblastoma patients is 15months. The development of this tumor depends on a sub-population of glioblastoma stem cells governing tumor propagation and therapy resistance. SOX3 plays a role in both normal neural development and carcinogenesis. However, little is known about its role in glioblastoma. Thus, the aim of this work was to elucidate the role of SOX3 in glioblastoma.MethodsSOX3 expression was assessed using real-time quantitative PCR (RT-qPCR), Western blotting and immunohistochemistry. MTT, immunocytochemistry and Transwell assays were used to evaluate the effects of exogenous SOX3 overexpression on the viability, proliferation, migration and invasion of glioblastoma cells, respectively. The expression of Hedgehog signaling pathway components and autophagy markers was assessed using RT-qPCR and Weste...rn blot analyses, respectively.ResultsHigher levels of SOX3 expression were detected in a subset of primary glioblastoma samples compared to those in non-tumoral brain tissues. Exogenous overexpression of this gene was found to increase the proliferation, viability, migration and invasion of glioblastoma cells. We also found that SOX3 up-regulation was accompanied by an enhanced activity of the Hedgehog signaling pathway and by suppression of autophagy in glioblastoma cells. Additionally, we found that SOX3 expression was elevated in patient-derived glioblastoma stem cells, as well as in oncospheres derived from glioblastoma cell lines, compared to their differentiated counterparts, implying that SOX3 expression is associated with the undifferentiated state of glioblastoma cells.ConclusionFrom our data we conclude that SOX3 can promote the malignant behavior of glioblastoma cells.
Ključne reči:
SOX3 / Migration / Hedgehog signaling / Glioblastoma / Glioblastoma stem cells / AutophagyIzvor:
Cellular Oncology, 2019, 42, 1, 41-54Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- Proučavanje signalnih puteva i epigenetičkih mehanizama uključenih u kontrolu ekspresije humanih SOX gena: dalje rasvetljavanje njihove uloge u određivanju sudbine i diferencijaciji ćelija (RS-MESTD-Basic Research (BR or ON)-173051)
- Serbian Academy of Sciences and Arts [F 24]
- IBRO-InEurope Short Stay Grants Program
DOI: 10.1007/s13402-018-0405-5
ISSN: 2211-3428
PubMed: 30209685
WoS: 000458495300004
Scopus: 2-s2.0-85053518133
Kolekcije
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Vicentić, Jelena Marjanovic AU - Drakulić, Danijela AU - Garcia, Idoia AU - Vuković, Vladanka AU - Aldaz, Paula AU - Puskas, Nela AU - Nikolić, Igor AU - Tasić, Goran AU - Raicević, Savo AU - Garros-Regulez, Laura AU - Sampron, Nicolas AU - Atkinson, Michael J. AU - Anastasov, Nataša AU - Matheu, Ander AU - Stevanović, Milena PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1285 AB - PurposeGlioblastoma is the most common and lethal adult brain tumor. Despite current therapeutic strategies, including surgery, radiation and chemotherapy, the median survival of glioblastoma patients is 15months. The development of this tumor depends on a sub-population of glioblastoma stem cells governing tumor propagation and therapy resistance. SOX3 plays a role in both normal neural development and carcinogenesis. However, little is known about its role in glioblastoma. Thus, the aim of this work was to elucidate the role of SOX3 in glioblastoma.MethodsSOX3 expression was assessed using real-time quantitative PCR (RT-qPCR), Western blotting and immunohistochemistry. MTT, immunocytochemistry and Transwell assays were used to evaluate the effects of exogenous SOX3 overexpression on the viability, proliferation, migration and invasion of glioblastoma cells, respectively. The expression of Hedgehog signaling pathway components and autophagy markers was assessed using RT-qPCR and Western blot analyses, respectively.ResultsHigher levels of SOX3 expression were detected in a subset of primary glioblastoma samples compared to those in non-tumoral brain tissues. Exogenous overexpression of this gene was found to increase the proliferation, viability, migration and invasion of glioblastoma cells. We also found that SOX3 up-regulation was accompanied by an enhanced activity of the Hedgehog signaling pathway and by suppression of autophagy in glioblastoma cells. Additionally, we found that SOX3 expression was elevated in patient-derived glioblastoma stem cells, as well as in oncospheres derived from glioblastoma cell lines, compared to their differentiated counterparts, implying that SOX3 expression is associated with the undifferentiated state of glioblastoma cells.ConclusionFrom our data we conclude that SOX3 can promote the malignant behavior of glioblastoma cells. PB - Springer, Dordrecht T2 - Cellular Oncology T1 - SOX3 can promote the malignant behavior of glioblastoma cells EP - 54 IS - 1 SP - 41 VL - 42 DO - 10.1007/s13402-018-0405-5 ER -
@article{ author = "Vicentić, Jelena Marjanovic and Drakulić, Danijela and Garcia, Idoia and Vuković, Vladanka and Aldaz, Paula and Puskas, Nela and Nikolić, Igor and Tasić, Goran and Raicević, Savo and Garros-Regulez, Laura and Sampron, Nicolas and Atkinson, Michael J. and Anastasov, Nataša and Matheu, Ander and Stevanović, Milena", year = "2019", abstract = "PurposeGlioblastoma is the most common and lethal adult brain tumor. Despite current therapeutic strategies, including surgery, radiation and chemotherapy, the median survival of glioblastoma patients is 15months. The development of this tumor depends on a sub-population of glioblastoma stem cells governing tumor propagation and therapy resistance. SOX3 plays a role in both normal neural development and carcinogenesis. However, little is known about its role in glioblastoma. Thus, the aim of this work was to elucidate the role of SOX3 in glioblastoma.MethodsSOX3 expression was assessed using real-time quantitative PCR (RT-qPCR), Western blotting and immunohistochemistry. MTT, immunocytochemistry and Transwell assays were used to evaluate the effects of exogenous SOX3 overexpression on the viability, proliferation, migration and invasion of glioblastoma cells, respectively. The expression of Hedgehog signaling pathway components and autophagy markers was assessed using RT-qPCR and Western blot analyses, respectively.ResultsHigher levels of SOX3 expression were detected in a subset of primary glioblastoma samples compared to those in non-tumoral brain tissues. Exogenous overexpression of this gene was found to increase the proliferation, viability, migration and invasion of glioblastoma cells. We also found that SOX3 up-regulation was accompanied by an enhanced activity of the Hedgehog signaling pathway and by suppression of autophagy in glioblastoma cells. Additionally, we found that SOX3 expression was elevated in patient-derived glioblastoma stem cells, as well as in oncospheres derived from glioblastoma cell lines, compared to their differentiated counterparts, implying that SOX3 expression is associated with the undifferentiated state of glioblastoma cells.ConclusionFrom our data we conclude that SOX3 can promote the malignant behavior of glioblastoma cells.", publisher = "Springer, Dordrecht", journal = "Cellular Oncology", title = "SOX3 can promote the malignant behavior of glioblastoma cells", pages = "54-41", number = "1", volume = "42", doi = "10.1007/s13402-018-0405-5" }
Vicentić, J. M., Drakulić, D., Garcia, I., Vuković, V., Aldaz, P., Puskas, N., Nikolić, I., Tasić, G., Raicević, S., Garros-Regulez, L., Sampron, N., Atkinson, M. J., Anastasov, N., Matheu, A.,& Stevanović, M.. (2019). SOX3 can promote the malignant behavior of glioblastoma cells. in Cellular Oncology Springer, Dordrecht., 42(1), 41-54. https://doi.org/10.1007/s13402-018-0405-5
Vicentić JM, Drakulić D, Garcia I, Vuković V, Aldaz P, Puskas N, Nikolić I, Tasić G, Raicević S, Garros-Regulez L, Sampron N, Atkinson MJ, Anastasov N, Matheu A, Stevanović M. SOX3 can promote the malignant behavior of glioblastoma cells. in Cellular Oncology. 2019;42(1):41-54. doi:10.1007/s13402-018-0405-5 .
Vicentić, Jelena Marjanovic, Drakulić, Danijela, Garcia, Idoia, Vuković, Vladanka, Aldaz, Paula, Puskas, Nela, Nikolić, Igor, Tasić, Goran, Raicević, Savo, Garros-Regulez, Laura, Sampron, Nicolas, Atkinson, Michael J., Anastasov, Nataša, Matheu, Ander, Stevanović, Milena, "SOX3 can promote the malignant behavior of glioblastoma cells" in Cellular Oncology, 42, no. 1 (2019):41-54, https://doi.org/10.1007/s13402-018-0405-5 . .