Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma
Нема приказа
Аутори
Petrović, JovanaGlamoclija, Jasmina
Ilić-Tomić, Tatjana
Soković, Marina
Robajac, Dragana
Nedić, Olgica
Pavić, Aleksandar
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In spite of extensive usage of Laetiporus sulphureus (sulphur polypore) in traditional European and Asian ethnomedicine for centuries, its anticancer therapeutic potential and toxicity profile remained explored in animal models. Herein, using zebrafish (Danio rerio), as a preclinical animal model, we demonstrated that L sulphureus lectin (LSL) and ethanol extract (LSE) are non-toxic at high doses up to 400-500 mu g/mL, while they effectively inhibited angiogenesis and cancer development at much lower doses. Lectin showed 74-fold higher antiangiogenic potency than the extract, and even 378-fold higher therapeutic potential than sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of clinical relevance. Using wound healing and MTT assays, we proved LSL's strong anti-migratory effect and selective endothelial cytotoxidty in relation to lung fibro-blasts. In addition, employing the zebrafish xenograft models, we demonstrated that LSL almost completely reduced growth, neov...ascularization and metastasis of human colorectal carcinoma and mouse melanoma. Even more, LSL exerted 8-fold higher potency towards colorectal carcinoma than melanoma, showing markedly higher activity than cisplatin, while LSE failed to express any anticancer activity. Accompanied with non-toxic response, including neutropenia and inflammation, the results of this study strongly imply that LSL could be used as safe adjuvant in chemotherapy against colorectal carcinoma and melanoma.
Кључне речи:
Zebrafish / Lectin / Laetiporus sulphureus / in vivo toxicity / in vivo anticancer activity / in vivo angiogenesisИзвор:
International Journal of Biological Macromolecules, 2020, 148, 129-139Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
- Структурне карактеристике везујућих протеина и рецептора за инсулину сличне факторе раста (IGF), њихове интеракције са другим физиолошким молекулима и промене код поремећаја метаболизма (RS-MESTD-Basic Research (BR or ON)-173042)
- Карактеризација и примена метаболита гљива и утврђивање потенцијала нових биофунгицида (RS-MESTD-Basic Research (BR or ON)-173032)
DOI: 10.1016/j.ijbiomac.2020.01.033
ISSN: 0141-8130
PubMed: 31935408
WoS: 000522094600014
Scopus: 2-s2.0-85077914300
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Petrović, Jovana AU - Glamoclija, Jasmina AU - Ilić-Tomić, Tatjana AU - Soković, Marina AU - Robajac, Dragana AU - Nedić, Olgica AU - Pavić, Aleksandar PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1383 AB - In spite of extensive usage of Laetiporus sulphureus (sulphur polypore) in traditional European and Asian ethnomedicine for centuries, its anticancer therapeutic potential and toxicity profile remained explored in animal models. Herein, using zebrafish (Danio rerio), as a preclinical animal model, we demonstrated that L sulphureus lectin (LSL) and ethanol extract (LSE) are non-toxic at high doses up to 400-500 mu g/mL, while they effectively inhibited angiogenesis and cancer development at much lower doses. Lectin showed 74-fold higher antiangiogenic potency than the extract, and even 378-fold higher therapeutic potential than sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of clinical relevance. Using wound healing and MTT assays, we proved LSL's strong anti-migratory effect and selective endothelial cytotoxidty in relation to lung fibro-blasts. In addition, employing the zebrafish xenograft models, we demonstrated that LSL almost completely reduced growth, neovascularization and metastasis of human colorectal carcinoma and mouse melanoma. Even more, LSL exerted 8-fold higher potency towards colorectal carcinoma than melanoma, showing markedly higher activity than cisplatin, while LSE failed to express any anticancer activity. Accompanied with non-toxic response, including neutropenia and inflammation, the results of this study strongly imply that LSL could be used as safe adjuvant in chemotherapy against colorectal carcinoma and melanoma. PB - Elsevier, Amsterdam T2 - International Journal of Biological Macromolecules T1 - Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma EP - 139 SP - 129 VL - 148 DO - 10.1016/j.ijbiomac.2020.01.033 ER -
@article{ author = "Petrović, Jovana and Glamoclija, Jasmina and Ilić-Tomić, Tatjana and Soković, Marina and Robajac, Dragana and Nedić, Olgica and Pavić, Aleksandar", year = "2020", abstract = "In spite of extensive usage of Laetiporus sulphureus (sulphur polypore) in traditional European and Asian ethnomedicine for centuries, its anticancer therapeutic potential and toxicity profile remained explored in animal models. Herein, using zebrafish (Danio rerio), as a preclinical animal model, we demonstrated that L sulphureus lectin (LSL) and ethanol extract (LSE) are non-toxic at high doses up to 400-500 mu g/mL, while they effectively inhibited angiogenesis and cancer development at much lower doses. Lectin showed 74-fold higher antiangiogenic potency than the extract, and even 378-fold higher therapeutic potential than sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of clinical relevance. Using wound healing and MTT assays, we proved LSL's strong anti-migratory effect and selective endothelial cytotoxidty in relation to lung fibro-blasts. In addition, employing the zebrafish xenograft models, we demonstrated that LSL almost completely reduced growth, neovascularization and metastasis of human colorectal carcinoma and mouse melanoma. Even more, LSL exerted 8-fold higher potency towards colorectal carcinoma than melanoma, showing markedly higher activity than cisplatin, while LSE failed to express any anticancer activity. Accompanied with non-toxic response, including neutropenia and inflammation, the results of this study strongly imply that LSL could be used as safe adjuvant in chemotherapy against colorectal carcinoma and melanoma.", publisher = "Elsevier, Amsterdam", journal = "International Journal of Biological Macromolecules", title = "Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma", pages = "139-129", volume = "148", doi = "10.1016/j.ijbiomac.2020.01.033" }
Petrović, J., Glamoclija, J., Ilić-Tomić, T., Soković, M., Robajac, D., Nedić, O.,& Pavić, A.. (2020). Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma. in International Journal of Biological Macromolecules Elsevier, Amsterdam., 148, 129-139. https://doi.org/10.1016/j.ijbiomac.2020.01.033
Petrović J, Glamoclija J, Ilić-Tomić T, Soković M, Robajac D, Nedić O, Pavić A. Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma. in International Journal of Biological Macromolecules. 2020;148:129-139. doi:10.1016/j.ijbiomac.2020.01.033 .
Petrović, Jovana, Glamoclija, Jasmina, Ilić-Tomić, Tatjana, Soković, Marina, Robajac, Dragana, Nedić, Olgica, Pavić, Aleksandar, "Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma" in International Journal of Biological Macromolecules, 148 (2020):129-139, https://doi.org/10.1016/j.ijbiomac.2020.01.033 . .