The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis
Nema prikaza
Autori
Mijailović, IvaNikolić, Nadja
Đinić, Ana
Carkić, Jelena
Milinković, Iva
Perić, Mina
Janković, Sasha
Milašin, Jelena
Aleksić, Zoran
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Background The exact mechanisms of bone resorption in periodontitis have not been fully elucidated. The aims of this study were to analyze the expression of Notch signaling molecules, bone remodeling mediators, and pro-inflammatory cytokines in periodontitis patients and to determine their potential correlations. Methods The study included 130 individuals: 40 with aggressive periodontitis (AP group), 40 with chronic periodontitis (CP group), and 50 periodontally healthy controls. Total RNA was extracted from gingival crevicular fluid samples and relative gene expression of investigated molecules (Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1, TNF-alpha, IL-17, RANKL, and OPG) was determined by reverse transcriptase - real-time polymerase chain reaction (RT-qPCR). Results In AP group, a significant increase of Notch 2, TNF-alpha, IL-17 and RANKL and a significant decrease of Notch 1 and Jagged 1 expression were observed compared to control group (P = 0.023, P = 0.005, P = 0.030, and P = 0.00...1 P = 0.031 and P = 0.029, respectively). Notch 2 and RANKL were also overexpressed in CP group compared to controls (P = 0.001 and P = 0.011). Significant correlations were observed in AP group between expression levels of the analyzed genes. Conclusion The present findings implicate Notch 2 overexpression in the ethiopathogenesis of bone resorption in aggressive and chronic periodontitis. The down-regulation of Notch 1 and Jagged 1 and loss of their osteoprotective function might cause a more excessive osteoclast formation and contribute to greater osteolysis in aggressive periodontitis.
Ključne reči:
periodontitis / gene expression / bone remodeling / aggressive periodontitisIzvor:
Journal of Periodontology, 2020, 91, 4, 554-561Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Genetička kontrola i molekularni mehanizmi u malignim, inflamatornim i razvojnim patologijama orofacijalne regije (RS-MESTD-Basic Research (BR or ON)-175075)
- Interakcija etiopatogenetskih mehanizama parodontopatije i paeriimplantitisa sa sistemskim bolestima današnjice (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41008)
DOI: 10.1002/JPER.18-0755
ISSN: 0022-3492
PubMed: 31468527
WoS: 000489221100001
Scopus: 2-s2.0-85084167989
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mijailović, Iva AU - Nikolić, Nadja AU - Đinić, Ana AU - Carkić, Jelena AU - Milinković, Iva AU - Perić, Mina AU - Janković, Sasha AU - Milašin, Jelena AU - Aleksić, Zoran PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1397 AB - Background The exact mechanisms of bone resorption in periodontitis have not been fully elucidated. The aims of this study were to analyze the expression of Notch signaling molecules, bone remodeling mediators, and pro-inflammatory cytokines in periodontitis patients and to determine their potential correlations. Methods The study included 130 individuals: 40 with aggressive periodontitis (AP group), 40 with chronic periodontitis (CP group), and 50 periodontally healthy controls. Total RNA was extracted from gingival crevicular fluid samples and relative gene expression of investigated molecules (Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1, TNF-alpha, IL-17, RANKL, and OPG) was determined by reverse transcriptase - real-time polymerase chain reaction (RT-qPCR). Results In AP group, a significant increase of Notch 2, TNF-alpha, IL-17 and RANKL and a significant decrease of Notch 1 and Jagged 1 expression were observed compared to control group (P = 0.023, P = 0.005, P = 0.030, and P = 0.001 P = 0.031 and P = 0.029, respectively). Notch 2 and RANKL were also overexpressed in CP group compared to controls (P = 0.001 and P = 0.011). Significant correlations were observed in AP group between expression levels of the analyzed genes. Conclusion The present findings implicate Notch 2 overexpression in the ethiopathogenesis of bone resorption in aggressive and chronic periodontitis. The down-regulation of Notch 1 and Jagged 1 and loss of their osteoprotective function might cause a more excessive osteoclast formation and contribute to greater osteolysis in aggressive periodontitis. PB - Wiley, Hoboken T2 - Journal of Periodontology T1 - The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis EP - 561 IS - 4 SP - 554 VL - 91 DO - 10.1002/JPER.18-0755 ER -
@article{ author = "Mijailović, Iva and Nikolić, Nadja and Đinić, Ana and Carkić, Jelena and Milinković, Iva and Perić, Mina and Janković, Sasha and Milašin, Jelena and Aleksić, Zoran", year = "2020", abstract = "Background The exact mechanisms of bone resorption in periodontitis have not been fully elucidated. The aims of this study were to analyze the expression of Notch signaling molecules, bone remodeling mediators, and pro-inflammatory cytokines in periodontitis patients and to determine their potential correlations. Methods The study included 130 individuals: 40 with aggressive periodontitis (AP group), 40 with chronic periodontitis (CP group), and 50 periodontally healthy controls. Total RNA was extracted from gingival crevicular fluid samples and relative gene expression of investigated molecules (Notch 1, Notch 2, Jagged 1, Hes 1, Hey 1, TNF-alpha, IL-17, RANKL, and OPG) was determined by reverse transcriptase - real-time polymerase chain reaction (RT-qPCR). Results In AP group, a significant increase of Notch 2, TNF-alpha, IL-17 and RANKL and a significant decrease of Notch 1 and Jagged 1 expression were observed compared to control group (P = 0.023, P = 0.005, P = 0.030, and P = 0.001 P = 0.031 and P = 0.029, respectively). Notch 2 and RANKL were also overexpressed in CP group compared to controls (P = 0.001 and P = 0.011). Significant correlations were observed in AP group between expression levels of the analyzed genes. Conclusion The present findings implicate Notch 2 overexpression in the ethiopathogenesis of bone resorption in aggressive and chronic periodontitis. The down-regulation of Notch 1 and Jagged 1 and loss of their osteoprotective function might cause a more excessive osteoclast formation and contribute to greater osteolysis in aggressive periodontitis.", publisher = "Wiley, Hoboken", journal = "Journal of Periodontology", title = "The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis", pages = "561-554", number = "4", volume = "91", doi = "10.1002/JPER.18-0755" }
Mijailović, I., Nikolić, N., Đinić, A., Carkić, J., Milinković, I., Perić, M., Janković, S., Milašin, J.,& Aleksić, Z.. (2020). The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis. in Journal of Periodontology Wiley, Hoboken., 91(4), 554-561. https://doi.org/10.1002/JPER.18-0755
Mijailović I, Nikolić N, Đinić A, Carkić J, Milinković I, Perić M, Janković S, Milašin J, Aleksić Z. The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis. in Journal of Periodontology. 2020;91(4):554-561. doi:10.1002/JPER.18-0755 .
Mijailović, Iva, Nikolić, Nadja, Đinić, Ana, Carkić, Jelena, Milinković, Iva, Perić, Mina, Janković, Sasha, Milašin, Jelena, Aleksić, Zoran, "The down-regulation of Notch 1 signaling contributes to the severity of bone loss in aggressive periodontitis" in Journal of Periodontology, 91, no. 4 (2020):554-561, https://doi.org/10.1002/JPER.18-0755 . .