Improvement of the anti-Candida activity of itraconazole in the zebrafish infection model by its coordination to silver(I)
Nema prikaza
Autori
Stevanović, Nevena Lj.Glišić, Biljana
Vojnović, Sandra
Wadepohl, Hubert
Andrejević, Tina P.
Durić, Sonja Z.
Savić, Nada D.
Nikodinović-Runić, Jasmina
Djuran, Milos
Pavić, Aleksandar
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In order to develop a novel antifungal agent, we synthesized and completely structurally characterized the silver(I) complex with the known antimycotic itraconazole (itraco), [Ag(itraco-N)(2)]NO3 center dot H2O (Agitraco). The spectroscopic and crystallographic results revealed that, in this complex, two itraco ligands are monodentately coordinated to the Ag(I) ion via the triazole nitrogen atom forming a cationic [Ag(itraco-N)(2)]+ part, which is neutralized by the nitrate anion. The antifungal effect of silver(I) complex and itraconazole was evaluated against four different Candida species (C. albicans, C. glabrata, C. parapsilosis and C. krusei) by means of minimal inhibitory concentrations (MICs). Agitraco complex shows enhanced antifungal activity than itraco, being 2.3- and 4.5-fold more active against C. albicans and C. glabrata, respectively. The complex was also more efficient in inhibiting yeast to hyphae transition process in C. albicans, which is an important step in its pa...thogenesis. Part of the improved activity of Agitraco could be attributed to the greater induction of reactive oxygen species in Candida spp. with respect to itraco. The toxicity evaluation in the zebrafish model (Danio rerio) suggests that the Agitraco complex has better therapeutic profile and improved antifungal efficacy with respect to the parent drug, which were also proven in vivo using the zebrafish model of lethal disseminated candidiasis. Interaction of Agitraco with bovine serum albumin (BSA) was investigated with the aim to assess its binding affinity toward this biomolecule.
Ključne reči:
Silver(I) complexes / Itraconazole / Infection model / Danio rerio / Candida albicansIzvor:
Journal of Molecular Structure, 2021, 1232Izdavač:
- Elsevier, Amsterdam
Finansiranje / projekti:
- Serbian Academy of Sciences and Arts [01-2019-F65, F128]
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200122 (Univerzitet u Kragujevcu, Prirodno-matematički fakultet) (RS-MESTD-inst-2020-200122)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200378 (Institut za informacione tehnologije, Kragujevac) (RS-MESTD-inst-2020-200378)
DOI: 10.1016/j.molstruc.2021.130006
ISSN: 0022-2860
WoS: 000632855300012
Scopus: 2-s2.0-85100422416
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stevanović, Nevena Lj. AU - Glišić, Biljana AU - Vojnović, Sandra AU - Wadepohl, Hubert AU - Andrejević, Tina P. AU - Durić, Sonja Z. AU - Savić, Nada D. AU - Nikodinović-Runić, Jasmina AU - Djuran, Milos AU - Pavić, Aleksandar PY - 2021 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1424 AB - In order to develop a novel antifungal agent, we synthesized and completely structurally characterized the silver(I) complex with the known antimycotic itraconazole (itraco), [Ag(itraco-N)(2)]NO3 center dot H2O (Agitraco). The spectroscopic and crystallographic results revealed that, in this complex, two itraco ligands are monodentately coordinated to the Ag(I) ion via the triazole nitrogen atom forming a cationic [Ag(itraco-N)(2)]+ part, which is neutralized by the nitrate anion. The antifungal effect of silver(I) complex and itraconazole was evaluated against four different Candida species (C. albicans, C. glabrata, C. parapsilosis and C. krusei) by means of minimal inhibitory concentrations (MICs). Agitraco complex shows enhanced antifungal activity than itraco, being 2.3- and 4.5-fold more active against C. albicans and C. glabrata, respectively. The complex was also more efficient in inhibiting yeast to hyphae transition process in C. albicans, which is an important step in its pathogenesis. Part of the improved activity of Agitraco could be attributed to the greater induction of reactive oxygen species in Candida spp. with respect to itraco. The toxicity evaluation in the zebrafish model (Danio rerio) suggests that the Agitraco complex has better therapeutic profile and improved antifungal efficacy with respect to the parent drug, which were also proven in vivo using the zebrafish model of lethal disseminated candidiasis. Interaction of Agitraco with bovine serum albumin (BSA) was investigated with the aim to assess its binding affinity toward this biomolecule. PB - Elsevier, Amsterdam T2 - Journal of Molecular Structure T1 - Improvement of the anti-Candida activity of itraconazole in the zebrafish infection model by its coordination to silver(I) VL - 1232 DO - 10.1016/j.molstruc.2021.130006 ER -
@article{ author = "Stevanović, Nevena Lj. and Glišić, Biljana and Vojnović, Sandra and Wadepohl, Hubert and Andrejević, Tina P. and Durić, Sonja Z. and Savić, Nada D. and Nikodinović-Runić, Jasmina and Djuran, Milos and Pavić, Aleksandar", year = "2021", abstract = "In order to develop a novel antifungal agent, we synthesized and completely structurally characterized the silver(I) complex with the known antimycotic itraconazole (itraco), [Ag(itraco-N)(2)]NO3 center dot H2O (Agitraco). The spectroscopic and crystallographic results revealed that, in this complex, two itraco ligands are monodentately coordinated to the Ag(I) ion via the triazole nitrogen atom forming a cationic [Ag(itraco-N)(2)]+ part, which is neutralized by the nitrate anion. The antifungal effect of silver(I) complex and itraconazole was evaluated against four different Candida species (C. albicans, C. glabrata, C. parapsilosis and C. krusei) by means of minimal inhibitory concentrations (MICs). Agitraco complex shows enhanced antifungal activity than itraco, being 2.3- and 4.5-fold more active against C. albicans and C. glabrata, respectively. The complex was also more efficient in inhibiting yeast to hyphae transition process in C. albicans, which is an important step in its pathogenesis. Part of the improved activity of Agitraco could be attributed to the greater induction of reactive oxygen species in Candida spp. with respect to itraco. The toxicity evaluation in the zebrafish model (Danio rerio) suggests that the Agitraco complex has better therapeutic profile and improved antifungal efficacy with respect to the parent drug, which were also proven in vivo using the zebrafish model of lethal disseminated candidiasis. Interaction of Agitraco with bovine serum albumin (BSA) was investigated with the aim to assess its binding affinity toward this biomolecule.", publisher = "Elsevier, Amsterdam", journal = "Journal of Molecular Structure", title = "Improvement of the anti-Candida activity of itraconazole in the zebrafish infection model by its coordination to silver(I)", volume = "1232", doi = "10.1016/j.molstruc.2021.130006" }
Stevanović, N. Lj., Glišić, B., Vojnović, S., Wadepohl, H., Andrejević, T. P., Durić, S. Z., Savić, N. D., Nikodinović-Runić, J., Djuran, M.,& Pavić, A.. (2021). Improvement of the anti-Candida activity of itraconazole in the zebrafish infection model by its coordination to silver(I). in Journal of Molecular Structure Elsevier, Amsterdam., 1232. https://doi.org/10.1016/j.molstruc.2021.130006
Stevanović NL, Glišić B, Vojnović S, Wadepohl H, Andrejević TP, Durić SZ, Savić ND, Nikodinović-Runić J, Djuran M, Pavić A. Improvement of the anti-Candida activity of itraconazole in the zebrafish infection model by its coordination to silver(I). in Journal of Molecular Structure. 2021;1232. doi:10.1016/j.molstruc.2021.130006 .
Stevanović, Nevena Lj., Glišić, Biljana, Vojnović, Sandra, Wadepohl, Hubert, Andrejević, Tina P., Durić, Sonja Z., Savić, Nada D., Nikodinović-Runić, Jasmina, Djuran, Milos , Pavić, Aleksandar, "Improvement of the anti-Candida activity of itraconazole in the zebrafish infection model by its coordination to silver(I)" in Journal of Molecular Structure, 1232 (2021), https://doi.org/10.1016/j.molstruc.2021.130006 . .