Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy
Аутори
Dinić, MiroslavHerholz, Marija
Kacarević, Uros
Radojević, Dušan
Novović, Katarina
Đokić, Jelena
Trifunović, Aleksandra
Golić, Nataša
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Gut homeostasis is maintained by the close interaction between commensal intestinal microbiota and the host, affecting the most complex physiological processes, such as aging. Some commensal bacteria with the potential to promote healthy aging arise as attractive candidates for the development of pro-longevity probiotics. Here, we showed that heat-inactivated human commensal Lactobacillus fermentum BGHV110 (BGHV110) extends the lifespan of Caenorhabditis elegans and improves age-related physiological features, including locomotor function and lipid metabolism. Mechanistically, we found that BGHV110 promotes HLH30/TFEB-dependent autophagy to delay aging, as longevity assurance was completely abolished in the mutant lacking HLH-30, a major autophagy regulator in C. elegans. Moreover, we observed that BGHV110 partially decreased the content of lipid droplets in an HLH-30-dependent manner and, at the same time, slightly increased mitochondrial activity. In summary, this study demonstrates ...that specific factors from commensal bacteria can be used to exploit HLH-30/TFEB-mediated autophagy in order to promote longevity and fitness of the host.
Кључне речи:
Lactobacillus fermentum / HLH-30 / Caenorhabditis elegans / autophagy / agingИзвор:
Aging, 2021, 13, 6, 8040-8054Издавач:
- Impact Journals LLC
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- A. Trifunovic's grant of the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [SFB 1218, 269925409]
- Federation of European Microbiological Societies (FEMS) [FEMS-GO-2017-016]
DOI: 10.18632/aging.202885
ISSN: 1945-4589
PubMed: 33770762
WoS: 000635963500023
Scopus: 2-s2.0-85103992901
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Dinić, Miroslav AU - Herholz, Marija AU - Kacarević, Uros AU - Radojević, Dušan AU - Novović, Katarina AU - Đokić, Jelena AU - Trifunović, Aleksandra AU - Golić, Nataša PY - 2021 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1431 AB - Gut homeostasis is maintained by the close interaction between commensal intestinal microbiota and the host, affecting the most complex physiological processes, such as aging. Some commensal bacteria with the potential to promote healthy aging arise as attractive candidates for the development of pro-longevity probiotics. Here, we showed that heat-inactivated human commensal Lactobacillus fermentum BGHV110 (BGHV110) extends the lifespan of Caenorhabditis elegans and improves age-related physiological features, including locomotor function and lipid metabolism. Mechanistically, we found that BGHV110 promotes HLH30/TFEB-dependent autophagy to delay aging, as longevity assurance was completely abolished in the mutant lacking HLH-30, a major autophagy regulator in C. elegans. Moreover, we observed that BGHV110 partially decreased the content of lipid droplets in an HLH-30-dependent manner and, at the same time, slightly increased mitochondrial activity. In summary, this study demonstrates that specific factors from commensal bacteria can be used to exploit HLH-30/TFEB-mediated autophagy in order to promote longevity and fitness of the host. PB - Impact Journals LLC T2 - Aging T1 - Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy EP - 8054 IS - 6 SP - 8040 VL - 13 DO - 10.18632/aging.202885 ER -
@article{ author = "Dinić, Miroslav and Herholz, Marija and Kacarević, Uros and Radojević, Dušan and Novović, Katarina and Đokić, Jelena and Trifunović, Aleksandra and Golić, Nataša", year = "2021", abstract = "Gut homeostasis is maintained by the close interaction between commensal intestinal microbiota and the host, affecting the most complex physiological processes, such as aging. Some commensal bacteria with the potential to promote healthy aging arise as attractive candidates for the development of pro-longevity probiotics. Here, we showed that heat-inactivated human commensal Lactobacillus fermentum BGHV110 (BGHV110) extends the lifespan of Caenorhabditis elegans and improves age-related physiological features, including locomotor function and lipid metabolism. Mechanistically, we found that BGHV110 promotes HLH30/TFEB-dependent autophagy to delay aging, as longevity assurance was completely abolished in the mutant lacking HLH-30, a major autophagy regulator in C. elegans. Moreover, we observed that BGHV110 partially decreased the content of lipid droplets in an HLH-30-dependent manner and, at the same time, slightly increased mitochondrial activity. In summary, this study demonstrates that specific factors from commensal bacteria can be used to exploit HLH-30/TFEB-mediated autophagy in order to promote longevity and fitness of the host.", publisher = "Impact Journals LLC", journal = "Aging", title = "Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy", pages = "8054-8040", number = "6", volume = "13", doi = "10.18632/aging.202885" }
Dinić, M., Herholz, M., Kacarević, U., Radojević, D., Novović, K., Đokić, J., Trifunović, A.,& Golić, N.. (2021). Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy. in Aging Impact Journals LLC., 13(6), 8040-8054. https://doi.org/10.18632/aging.202885
Dinić M, Herholz M, Kacarević U, Radojević D, Novović K, Đokić J, Trifunović A, Golić N. Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy. in Aging. 2021;13(6):8040-8054. doi:10.18632/aging.202885 .
Dinić, Miroslav, Herholz, Marija, Kacarević, Uros, Radojević, Dušan, Novović, Katarina, Đokić, Jelena, Trifunović, Aleksandra, Golić, Nataša, "Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy" in Aging, 13, no. 6 (2021):8040-8054, https://doi.org/10.18632/aging.202885 . .