Case Report: Successful Therapy of Spontaneously Occurring Canine Degenerative Lumbosacral Stenosis Using Autologous Adipose Tissue-Derived Mesenchymal Stem Cells
2021
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Authors
Mrkovacki, JankoSrzentić Dražilov, Sanja
Spasovski, Vesna
Fazlagić, Amira
Pavlović, Sonja
Nikčević, Gordana
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The management of degenerative lumbosacral stenosis (DLSS) in dogs usually requires aggressive, costly surgical treatments that may themselves present complications, while do not fully resolve the symptoms of the disease. In this study, the dog diagnosed with severe DLSS, with hind limb paresis, was treated using a new and least invasive treatment. Cultured autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) were injected bilaterally at the level of L7-S1, in the vicinity of the external aperture of the intervertebral foramen of DLSS patient. In the previously described treatments of spontaneous intervertebral disc degeneration in dogs, intradiscal injections of MSCs did not lead to positive effects. Here, we report a marked improvement in clinical outcome measures related to the ability of a dog to walk and trot, which were expressed by a numeric rating scale based on a veterinary assessment questionnaire. The improved status persisted throughout the observed time cours...e of 4.5 years after the AT-MSC transplantation. To the best of our knowledge, this is the first case of successful therapy, with long-term positive effect, of spontaneously occurring canine DLSS using presented treatment that, we believe, represents a contribution to current knowledge in this field and may shape both animal and human DLSS treatment options.
Keywords:
regenerative medicine / minimally invasive treatment / degenerative lumbosacral stenosis / case report / canine (dog) / autologous AT-MSCsSource:
Frontiers in Veterinary Science, 2021, 8Publisher:
- Frontiers Media Sa, Lausanne
Funding / projects:
- Rare Diseases:Molecular Pathophysiology, Diagnostic and Therapeutic Modalities and Social, Ethical and Legal Aspects (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41004)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
DOI: 10.3389/fvets.2021.732073
ISSN: 2297-1769
WoS: 000705910800001
Scopus: 2-s2.0-85116621391
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mrkovacki, Janko AU - Srzentić Dražilov, Sanja AU - Spasovski, Vesna AU - Fazlagić, Amira AU - Pavlović, Sonja AU - Nikčević, Gordana PY - 2021 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1476 AB - The management of degenerative lumbosacral stenosis (DLSS) in dogs usually requires aggressive, costly surgical treatments that may themselves present complications, while do not fully resolve the symptoms of the disease. In this study, the dog diagnosed with severe DLSS, with hind limb paresis, was treated using a new and least invasive treatment. Cultured autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) were injected bilaterally at the level of L7-S1, in the vicinity of the external aperture of the intervertebral foramen of DLSS patient. In the previously described treatments of spontaneous intervertebral disc degeneration in dogs, intradiscal injections of MSCs did not lead to positive effects. Here, we report a marked improvement in clinical outcome measures related to the ability of a dog to walk and trot, which were expressed by a numeric rating scale based on a veterinary assessment questionnaire. The improved status persisted throughout the observed time course of 4.5 years after the AT-MSC transplantation. To the best of our knowledge, this is the first case of successful therapy, with long-term positive effect, of spontaneously occurring canine DLSS using presented treatment that, we believe, represents a contribution to current knowledge in this field and may shape both animal and human DLSS treatment options. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Veterinary Science T1 - Case Report: Successful Therapy of Spontaneously Occurring Canine Degenerative Lumbosacral Stenosis Using Autologous Adipose Tissue-Derived Mesenchymal Stem Cells VL - 8 DO - 10.3389/fvets.2021.732073 ER -
@article{ author = "Mrkovacki, Janko and Srzentić Dražilov, Sanja and Spasovski, Vesna and Fazlagić, Amira and Pavlović, Sonja and Nikčević, Gordana", year = "2021", abstract = "The management of degenerative lumbosacral stenosis (DLSS) in dogs usually requires aggressive, costly surgical treatments that may themselves present complications, while do not fully resolve the symptoms of the disease. In this study, the dog diagnosed with severe DLSS, with hind limb paresis, was treated using a new and least invasive treatment. Cultured autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) were injected bilaterally at the level of L7-S1, in the vicinity of the external aperture of the intervertebral foramen of DLSS patient. In the previously described treatments of spontaneous intervertebral disc degeneration in dogs, intradiscal injections of MSCs did not lead to positive effects. Here, we report a marked improvement in clinical outcome measures related to the ability of a dog to walk and trot, which were expressed by a numeric rating scale based on a veterinary assessment questionnaire. The improved status persisted throughout the observed time course of 4.5 years after the AT-MSC transplantation. To the best of our knowledge, this is the first case of successful therapy, with long-term positive effect, of spontaneously occurring canine DLSS using presented treatment that, we believe, represents a contribution to current knowledge in this field and may shape both animal and human DLSS treatment options.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Veterinary Science", title = "Case Report: Successful Therapy of Spontaneously Occurring Canine Degenerative Lumbosacral Stenosis Using Autologous Adipose Tissue-Derived Mesenchymal Stem Cells", volume = "8", doi = "10.3389/fvets.2021.732073" }
Mrkovacki, J., Srzentić Dražilov, S., Spasovski, V., Fazlagić, A., Pavlović, S.,& Nikčević, G.. (2021). Case Report: Successful Therapy of Spontaneously Occurring Canine Degenerative Lumbosacral Stenosis Using Autologous Adipose Tissue-Derived Mesenchymal Stem Cells. in Frontiers in Veterinary Science Frontiers Media Sa, Lausanne., 8. https://doi.org/10.3389/fvets.2021.732073
Mrkovacki J, Srzentić Dražilov S, Spasovski V, Fazlagić A, Pavlović S, Nikčević G. Case Report: Successful Therapy of Spontaneously Occurring Canine Degenerative Lumbosacral Stenosis Using Autologous Adipose Tissue-Derived Mesenchymal Stem Cells. in Frontiers in Veterinary Science. 2021;8. doi:10.3389/fvets.2021.732073 .
Mrkovacki, Janko, Srzentić Dražilov, Sanja, Spasovski, Vesna, Fazlagić, Amira, Pavlović, Sonja, Nikčević, Gordana, "Case Report: Successful Therapy of Spontaneously Occurring Canine Degenerative Lumbosacral Stenosis Using Autologous Adipose Tissue-Derived Mesenchymal Stem Cells" in Frontiers in Veterinary Science, 8 (2021), https://doi.org/10.3389/fvets.2021.732073 . .