Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line
Authors
Stojković, DejanDrakulić, Danijela
Dias, Maria Ines
Zengin, Gokhan
Barros, Lillian
Ivanov, Marija
Gašić, Uroš
Rajcević, Nemanja
Stevanović, Milena
Ferreira, Isabel C. F. R.
Soković, Marina
Article (Published version)
Metadata
Show full item recordAbstract
Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa ex-tract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic ef-fect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 mu g/mL. The extract did not induce changes i...n proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.
Keywords:
prooxidant / Phlomis fruticosa / phenolic compounds / glioblastoma / antioxidant / antineurodegenerativeSource:
EXCLI Journal, 2022, 21, 387-399Publisher:
- EXCLI Journal Managing Office, Dortmund
Funding / projects:
- Foundation for Science and Technology (FCT, Portugal) [UIDB/00690/2020]
- FEDER under Program PT2020 [UIDB/00690/2020]
- FCT, P.I.
- FEDER-Interreg Espana-Portugal program [0377_Iberphenol_6_E, TRANSCoLAB 0612_TRANS_CO_LAB_2_P]
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
DOI: 10.17179/excli2021-4487
ISSN: 1611-2156
WoS: 000755071100001
Scopus: 2-s2.0-85125086745
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stojković, Dejan AU - Drakulić, Danijela AU - Dias, Maria Ines AU - Zengin, Gokhan AU - Barros, Lillian AU - Ivanov, Marija AU - Gašić, Uroš AU - Rajcević, Nemanja AU - Stevanović, Milena AU - Ferreira, Isabel C. F. R. AU - Soković, Marina PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1574 AB - Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa ex-tract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic ef-fect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 mu g/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option. PB - EXCLI Journal Managing Office, Dortmund T2 - EXCLI Journal T1 - Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line EP - 399 SP - 387 VL - 21 DO - 10.17179/excli2021-4487 ER -
@article{ author = "Stojković, Dejan and Drakulić, Danijela and Dias, Maria Ines and Zengin, Gokhan and Barros, Lillian and Ivanov, Marija and Gašić, Uroš and Rajcević, Nemanja and Stevanović, Milena and Ferreira, Isabel C. F. R. and Soković, Marina", year = "2022", abstract = "Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa ex-tract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic ef-fect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 mu g/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.", publisher = "EXCLI Journal Managing Office, Dortmund", journal = "EXCLI Journal", title = "Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line", pages = "399-387", volume = "21", doi = "10.17179/excli2021-4487" }
Stojković, D., Drakulić, D., Dias, M. I., Zengin, G., Barros, L., Ivanov, M., Gašić, U., Rajcević, N., Stevanović, M., Ferreira, I. C. F. R.,& Soković, M.. (2022). Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line. in EXCLI Journal EXCLI Journal Managing Office, Dortmund., 21, 387-399. https://doi.org/10.17179/excli2021-4487
Stojković D, Drakulić D, Dias MI, Zengin G, Barros L, Ivanov M, Gašić U, Rajcević N, Stevanović M, Ferreira ICFR, Soković M. Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line. in EXCLI Journal. 2022;21:387-399. doi:10.17179/excli2021-4487 .
Stojković, Dejan, Drakulić, Danijela, Dias, Maria Ines, Zengin, Gokhan, Barros, Lillian, Ivanov, Marija, Gašić, Uroš, Rajcević, Nemanja, Stevanović, Milena, Ferreira, Isabel C. F. R., Soković, Marina, "Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line" in EXCLI Journal, 21 (2022):387-399, https://doi.org/10.17179/excli2021-4487 . .