Disruptions of the Ustilago maydis REC2 gene identify a protein domain important in directing recombinational repair of DNA
Apstrakt
The REC2 gene of Ustilago maydis encodes a homologue of the Escherichia coli RecA protein and was first identified in a screen for UV-sensitive mutants. The original isolate, rec2-1, was found to be deficient in repair of DNA damage, genetic recombination and meiosis. We report here that the rec2-197 allele, which was constructed by gene disruption, retains some biological activity and is partially dominant with respect to REC2. The basis for the residual activity is probably as a result of expression of a diffusible product from the rec2-197 allele that augments or interferes with REC2 functions. This product appears to be a polypeptide expressed from a remnant of the 5' end of the open reading frame that was not removed in creating the gene disruption. The mutator activity and disturbed meiosis of rec2-197 suggest that the Rec2 protein functions in a process that avoids spontaneous mutation and insures faithful meiotic chromosome segregation. A prediction based on the phenotype of re...c2-197 is that Rec2 protein interacts with one or more other proteins in directing these functions. To identify interacting proteins we performed a yeast two-hybrid screen and found Rad51 as a candidate. Rec2-197 and Rad51 appear to interact to a similar degree.
Izvor:
Molecular Microbiology, 2001, 40, 6, 1415-1426Izdavač:
- Blackwell Science Ltd, Oxford
Finansiranje / projekti:
- NIGMS NIH HHS [GM42482] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM042482] Funding Source: NIH RePORTER
DOI: 10.1046/j.1365-2958.2001.02490.x
ISSN: 0950-382X
PubMed: 11442839
WoS: 000169580400016
Scopus: 2-s2.0-0034933593
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Milorad AU - Thompson, CW AU - Holloman, WK PY - 2001 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/159 AB - The REC2 gene of Ustilago maydis encodes a homologue of the Escherichia coli RecA protein and was first identified in a screen for UV-sensitive mutants. The original isolate, rec2-1, was found to be deficient in repair of DNA damage, genetic recombination and meiosis. We report here that the rec2-197 allele, which was constructed by gene disruption, retains some biological activity and is partially dominant with respect to REC2. The basis for the residual activity is probably as a result of expression of a diffusible product from the rec2-197 allele that augments or interferes with REC2 functions. This product appears to be a polypeptide expressed from a remnant of the 5' end of the open reading frame that was not removed in creating the gene disruption. The mutator activity and disturbed meiosis of rec2-197 suggest that the Rec2 protein functions in a process that avoids spontaneous mutation and insures faithful meiotic chromosome segregation. A prediction based on the phenotype of rec2-197 is that Rec2 protein interacts with one or more other proteins in directing these functions. To identify interacting proteins we performed a yeast two-hybrid screen and found Rad51 as a candidate. Rec2-197 and Rad51 appear to interact to a similar degree. PB - Blackwell Science Ltd, Oxford T2 - Molecular Microbiology T1 - Disruptions of the Ustilago maydis REC2 gene identify a protein domain important in directing recombinational repair of DNA EP - 1426 IS - 6 SP - 1415 VL - 40 DO - 10.1046/j.1365-2958.2001.02490.x ER -
@article{ author = "Kojić, Milorad and Thompson, CW and Holloman, WK", year = "2001", abstract = "The REC2 gene of Ustilago maydis encodes a homologue of the Escherichia coli RecA protein and was first identified in a screen for UV-sensitive mutants. The original isolate, rec2-1, was found to be deficient in repair of DNA damage, genetic recombination and meiosis. We report here that the rec2-197 allele, which was constructed by gene disruption, retains some biological activity and is partially dominant with respect to REC2. The basis for the residual activity is probably as a result of expression of a diffusible product from the rec2-197 allele that augments or interferes with REC2 functions. This product appears to be a polypeptide expressed from a remnant of the 5' end of the open reading frame that was not removed in creating the gene disruption. The mutator activity and disturbed meiosis of rec2-197 suggest that the Rec2 protein functions in a process that avoids spontaneous mutation and insures faithful meiotic chromosome segregation. A prediction based on the phenotype of rec2-197 is that Rec2 protein interacts with one or more other proteins in directing these functions. To identify interacting proteins we performed a yeast two-hybrid screen and found Rad51 as a candidate. Rec2-197 and Rad51 appear to interact to a similar degree.", publisher = "Blackwell Science Ltd, Oxford", journal = "Molecular Microbiology", title = "Disruptions of the Ustilago maydis REC2 gene identify a protein domain important in directing recombinational repair of DNA", pages = "1426-1415", number = "6", volume = "40", doi = "10.1046/j.1365-2958.2001.02490.x" }
Kojić, M., Thompson, C.,& Holloman, W.. (2001). Disruptions of the Ustilago maydis REC2 gene identify a protein domain important in directing recombinational repair of DNA. in Molecular Microbiology Blackwell Science Ltd, Oxford., 40(6), 1415-1426. https://doi.org/10.1046/j.1365-2958.2001.02490.x
Kojić M, Thompson C, Holloman W. Disruptions of the Ustilago maydis REC2 gene identify a protein domain important in directing recombinational repair of DNA. in Molecular Microbiology. 2001;40(6):1415-1426. doi:10.1046/j.1365-2958.2001.02490.x .
Kojić, Milorad, Thompson, CW, Holloman, WK, "Disruptions of the Ustilago maydis REC2 gene identify a protein domain important in directing recombinational repair of DNA" in Molecular Microbiology, 40, no. 6 (2001):1415-1426, https://doi.org/10.1046/j.1365-2958.2001.02490.x . .