Antimicrobial and cytotoxic activity of pigmented Streptomyces spp. culture extracts
Аутори
Janković, VukašinNikodinović-Runić, Jasmina
Milivojević, Dušan
Stevanović, Milena
Ilić-Tomić, Tatjana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
BACKGROUND
Pigments from microbial origin have promising applications in food, cosmetics, textiles and therapeutics.
Compared to other natural sources pigments from bacteria are more stable, safer, and could be cheaper to
produce and extract. Bacterial genus Streptomyces has been known as a source of biologicaly active pigments
that exhibit several effects such as antimicrobial and cytotoxic.
OBJECTIVES
The aim of this study was the isolation of pigment-producing Streptomyces strain from soil, optimization of
growth parametres for pigment production and evaluation of antimicrobial and cytotoxic activity of extracted
bacterial pigments.
METHODS
Isolation and characterization of pigment-producing Streptomyces strain from soil was done following the
standard microbiological protocol, using four different growth media. The pigments were extracted by solvent
extraction using ethyl acetate. Antimicrobial activity was analyzed using the disc diffusion test. Cytotoxic activity
was... tested on the HaCaT cell line following the standard protocol.
RESULTS
Extraction of pigments by solvent extraction resulted in crude pigment extracts with antimicrobial activities.
In total pigments from 14 bacterial strains have been extracted. Antimicrobial activity was evident on Gram +
bacteria Staphylococcus aureus (6 mm of inhibition zone at concentration 8.4 μg/ml and 4 mm of inhibition
zone at concentration 1.1 μg/ml) as well as on the fungus Candida albicans (4 mm of inhibition zone at concentration
13 μg/ml). Pigments showed dose-dependent inhibiton of proliferation of HaCaT cells, with the
lowest concentration at 25 μg/ml.
Кључне речи:
bacterial pigments / antimicrobial / cytotoxic / StreptomycesИзвор:
FEMS Conference on Microbiology, 2022, 621-622Финансирање / пројекти:
- Bio Innovation of a Circular Economy for Plastics (BioICEP) Project H2020-870292, 2020-2023
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Напомена:
- ELECTRONIC ABSTRACT BOOK: FEMS Conference on Microbiology in association with Serbian Society of Microbiology 30 June - 2 July 2022; Serbia
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Janković, Vukašin AU - Nikodinović-Runić, Jasmina AU - Milivojević, Dušan AU - Stevanović, Milena AU - Ilić-Tomić, Tatjana PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1843 AB - BACKGROUND Pigments from microbial origin have promising applications in food, cosmetics, textiles and therapeutics. Compared to other natural sources pigments from bacteria are more stable, safer, and could be cheaper to produce and extract. Bacterial genus Streptomyces has been known as a source of biologicaly active pigments that exhibit several effects such as antimicrobial and cytotoxic. OBJECTIVES The aim of this study was the isolation of pigment-producing Streptomyces strain from soil, optimization of growth parametres for pigment production and evaluation of antimicrobial and cytotoxic activity of extracted bacterial pigments. METHODS Isolation and characterization of pigment-producing Streptomyces strain from soil was done following the standard microbiological protocol, using four different growth media. The pigments were extracted by solvent extraction using ethyl acetate. Antimicrobial activity was analyzed using the disc diffusion test. Cytotoxic activity was tested on the HaCaT cell line following the standard protocol. RESULTS Extraction of pigments by solvent extraction resulted in crude pigment extracts with antimicrobial activities. In total pigments from 14 bacterial strains have been extracted. Antimicrobial activity was evident on Gram + bacteria Staphylococcus aureus (6 mm of inhibition zone at concentration 8.4 μg/ml and 4 mm of inhibition zone at concentration 1.1 μg/ml) as well as on the fungus Candida albicans (4 mm of inhibition zone at concentration 13 μg/ml). Pigments showed dose-dependent inhibiton of proliferation of HaCaT cells, with the lowest concentration at 25 μg/ml. C3 - FEMS Conference on Microbiology T1 - Antimicrobial and cytotoxic activity of pigmented Streptomyces spp. culture extracts EP - 622 SP - 621 UR - https://hdl.handle.net/21.15107/rcub_imagine_1843 ER -
@conference{ author = "Janković, Vukašin and Nikodinović-Runić, Jasmina and Milivojević, Dušan and Stevanović, Milena and Ilić-Tomić, Tatjana", year = "2022", abstract = "BACKGROUND Pigments from microbial origin have promising applications in food, cosmetics, textiles and therapeutics. Compared to other natural sources pigments from bacteria are more stable, safer, and could be cheaper to produce and extract. Bacterial genus Streptomyces has been known as a source of biologicaly active pigments that exhibit several effects such as antimicrobial and cytotoxic. OBJECTIVES The aim of this study was the isolation of pigment-producing Streptomyces strain from soil, optimization of growth parametres for pigment production and evaluation of antimicrobial and cytotoxic activity of extracted bacterial pigments. METHODS Isolation and characterization of pigment-producing Streptomyces strain from soil was done following the standard microbiological protocol, using four different growth media. The pigments were extracted by solvent extraction using ethyl acetate. Antimicrobial activity was analyzed using the disc diffusion test. Cytotoxic activity was tested on the HaCaT cell line following the standard protocol. RESULTS Extraction of pigments by solvent extraction resulted in crude pigment extracts with antimicrobial activities. In total pigments from 14 bacterial strains have been extracted. Antimicrobial activity was evident on Gram + bacteria Staphylococcus aureus (6 mm of inhibition zone at concentration 8.4 μg/ml and 4 mm of inhibition zone at concentration 1.1 μg/ml) as well as on the fungus Candida albicans (4 mm of inhibition zone at concentration 13 μg/ml). Pigments showed dose-dependent inhibiton of proliferation of HaCaT cells, with the lowest concentration at 25 μg/ml.", journal = "FEMS Conference on Microbiology", title = "Antimicrobial and cytotoxic activity of pigmented Streptomyces spp. culture extracts", pages = "622-621", url = "https://hdl.handle.net/21.15107/rcub_imagine_1843" }
Janković, V., Nikodinović-Runić, J., Milivojević, D., Stevanović, M.,& Ilić-Tomić, T.. (2022). Antimicrobial and cytotoxic activity of pigmented Streptomyces spp. culture extracts. in FEMS Conference on Microbiology, 621-622. https://hdl.handle.net/21.15107/rcub_imagine_1843
Janković V, Nikodinović-Runić J, Milivojević D, Stevanović M, Ilić-Tomić T. Antimicrobial and cytotoxic activity of pigmented Streptomyces spp. culture extracts. in FEMS Conference on Microbiology. 2022;:621-622. https://hdl.handle.net/21.15107/rcub_imagine_1843 .
Janković, Vukašin, Nikodinović-Runić, Jasmina, Milivojević, Dušan, Stevanović, Milena, Ilić-Tomić, Tatjana, "Antimicrobial and cytotoxic activity of pigmented Streptomyces spp. culture extracts" in FEMS Conference on Microbiology (2022):621-622, https://hdl.handle.net/21.15107/rcub_imagine_1843 .