Expression of PET-hydrolyzing enzymes in Streptomyces spp.
Authors
Stevanović, Milena![](/themes/Mirageimagine/images/orcid.png)
Pantelić, Brana
![](/themes/Mirageimagine/images/orcid.png)
Janković, Vukašin
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Nikodinović-Runić, Jasmina
![](/themes/Mirageimagine/images/orcid.png)
Vojnović, Sandra
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Show full item recordAbstract
Plastic waste has become a serious global challenge that calls for sustainable solutions and requires
rapid actions. Biocatalysis could present an adequate answer to this problem by providing different
enzymes capable of degrading plastic polymers. Streptomyces strains as predominant soil
inhabitants have also adapted to the presence of variety of plastic waste in natural environments,
so they have been examined for the plastic degrading capabilities.
The aim of this work was to improve the biocatalytic properties of Streptomyces strains for their
use in biodegradation of plastic polymers and develop a system for heterologous expression of
polyethylene therephtalate (PET) degrading enzymes in Streptomyces spp.
Well studied Streptomyces lividans TK24 and S. albus NRRL B-1335, as well as two newly
isolated Streptomycetes were used for expression of benchmark PETases and cutinases. Enzymes
were cloned into pGM1202 Escherichia coli–Streptomyces shuttle vector and subsequently
in...troduced into Streptomyces hosts either by polyethylene glycol-mediated protoplasts
transformation or by electroporation. Cell-free extracts and supernatants of transformed cells were
tested on different plastics using bis(2-hydroxyethyl) terephthalate (BHET), polycaprolactone
(PCL) and Impranil as substrates in plate assays.
Expression of leaf-branch compost cutinase in S. albus and S. lividans resulted in an 8.5- and 2.5-
times increase in esterase activities, respectively. Introduction of the enzyme into newly isolated
strains that already showed some plastic degrading activity resulted in synergistic activity of the
recombinant strains.
Keywords:
streptomyces / enzyme / PET / heterologous expressionSource:
FEMS2023 Congress of European Microbiologists, 2023, 10, 836-836Note:
- 10th FEMS Congress of European Microbiologists, Hamburg, Germany from July 9 -13th, 2023.
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Stevanović, Milena AU - Pantelić, Brana AU - Janković, Vukašin AU - Nikodinović-Runić, Jasmina AU - Vojnović, Sandra PY - 2023 UR - https://www.fems2023.org/ UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2022 AB - Plastic waste has become a serious global challenge that calls for sustainable solutions and requires rapid actions. Biocatalysis could present an adequate answer to this problem by providing different enzymes capable of degrading plastic polymers. Streptomyces strains as predominant soil inhabitants have also adapted to the presence of variety of plastic waste in natural environments, so they have been examined for the plastic degrading capabilities. The aim of this work was to improve the biocatalytic properties of Streptomyces strains for their use in biodegradation of plastic polymers and develop a system for heterologous expression of polyethylene therephtalate (PET) degrading enzymes in Streptomyces spp. Well studied Streptomyces lividans TK24 and S. albus NRRL B-1335, as well as two newly isolated Streptomycetes were used for expression of benchmark PETases and cutinases. Enzymes were cloned into pGM1202 Escherichia coli–Streptomyces shuttle vector and subsequently introduced into Streptomyces hosts either by polyethylene glycol-mediated protoplasts transformation or by electroporation. Cell-free extracts and supernatants of transformed cells were tested on different plastics using bis(2-hydroxyethyl) terephthalate (BHET), polycaprolactone (PCL) and Impranil as substrates in plate assays. Expression of leaf-branch compost cutinase in S. albus and S. lividans resulted in an 8.5- and 2.5- times increase in esterase activities, respectively. Introduction of the enzyme into newly isolated strains that already showed some plastic degrading activity resulted in synergistic activity of the recombinant strains. C3 - FEMS2023 Congress of European Microbiologists T1 - Expression of PET-hydrolyzing enzymes in Streptomyces spp. EP - 836 SP - 836 VL - 10 UR - https://hdl.handle.net/21.15107/rcub_imagine_2022 ER -
@conference{ author = "Stevanović, Milena and Pantelić, Brana and Janković, Vukašin and Nikodinović-Runić, Jasmina and Vojnović, Sandra", year = "2023", abstract = "Plastic waste has become a serious global challenge that calls for sustainable solutions and requires rapid actions. Biocatalysis could present an adequate answer to this problem by providing different enzymes capable of degrading plastic polymers. Streptomyces strains as predominant soil inhabitants have also adapted to the presence of variety of plastic waste in natural environments, so they have been examined for the plastic degrading capabilities. The aim of this work was to improve the biocatalytic properties of Streptomyces strains for their use in biodegradation of plastic polymers and develop a system for heterologous expression of polyethylene therephtalate (PET) degrading enzymes in Streptomyces spp. Well studied Streptomyces lividans TK24 and S. albus NRRL B-1335, as well as two newly isolated Streptomycetes were used for expression of benchmark PETases and cutinases. Enzymes were cloned into pGM1202 Escherichia coli–Streptomyces shuttle vector and subsequently introduced into Streptomyces hosts either by polyethylene glycol-mediated protoplasts transformation or by electroporation. Cell-free extracts and supernatants of transformed cells were tested on different plastics using bis(2-hydroxyethyl) terephthalate (BHET), polycaprolactone (PCL) and Impranil as substrates in plate assays. Expression of leaf-branch compost cutinase in S. albus and S. lividans resulted in an 8.5- and 2.5- times increase in esterase activities, respectively. Introduction of the enzyme into newly isolated strains that already showed some plastic degrading activity resulted in synergistic activity of the recombinant strains.", journal = "FEMS2023 Congress of European Microbiologists", title = "Expression of PET-hydrolyzing enzymes in Streptomyces spp.", pages = "836-836", volume = "10", url = "https://hdl.handle.net/21.15107/rcub_imagine_2022" }
Stevanović, M., Pantelić, B., Janković, V., Nikodinović-Runić, J.,& Vojnović, S.. (2023). Expression of PET-hydrolyzing enzymes in Streptomyces spp.. in FEMS2023 Congress of European Microbiologists, 10, 836-836. https://hdl.handle.net/21.15107/rcub_imagine_2022
Stevanović M, Pantelić B, Janković V, Nikodinović-Runić J, Vojnović S. Expression of PET-hydrolyzing enzymes in Streptomyces spp.. in FEMS2023 Congress of European Microbiologists. 2023;10:836-836. https://hdl.handle.net/21.15107/rcub_imagine_2022 .
Stevanović, Milena, Pantelić, Brana, Janković, Vukašin, Nikodinović-Runić, Jasmina, Vojnović, Sandra, "Expression of PET-hydrolyzing enzymes in Streptomyces spp." in FEMS2023 Congress of European Microbiologists, 10 (2023):836-836, https://hdl.handle.net/21.15107/rcub_imagine_2022 .