Protoplast patch-clamping using an upright microscope with a movable stage
Authors
Nikolić, LjiljanaVidović, Marija
Todorović, Nataša
Petković, Branka
Stojadinović, Gordana
Martać, Ljiljana
Bogdanović Pristov, Jelena
Contributors
Čupić, ŽeljkoAnić, Slobodan
Conference object (Published version)
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© 2022 by the Society of Physical Chemists of Serbia
Metadata
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Investigating membrane properties of plants is a challenging task, considering that success ofexperiments is highly dependent on the possibility to isolate metabolically active protoplasts thatcan withstand membrane current recordings. The aim of the present work is to obtain viableprotoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patchclamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplastswith preserved membrane integrity suitable for electrophysiological experiments. We applied acustom approach for patch-clamping protoplasts using a microscope with a movable microscopestage. We recorded prominent inward and prominent outward types of membrane current profiles ofprotoplasts. Obtained data indicate that optimized isolation protocol and custom system for patchclamping, can be applied to study membrane properties of root protoplasts.
Keywords:
plant protoplast / patch-clamp / microscopeSource:
Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia, 2022, 271-274Publisher:
- Belgrade : Society of Physical Chemists of Serbia
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-MESTD-inst-2020-200053)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
Note:
- Čupić Ž, Anić S, editors. Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia. Belgrade: Society of Physical Chemists of Serbia; 2022. p. 271-4.
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Nikolić, Ljiljana AU - Vidović, Marija AU - Todorović, Nataša AU - Petković, Branka AU - Stojadinović, Gordana AU - Martać, Ljiljana AU - Bogdanović Pristov, Jelena PY - 2022 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2168 AB - Investigating membrane properties of plants is a challenging task, considering that success ofexperiments is highly dependent on the possibility to isolate metabolically active protoplasts thatcan withstand membrane current recordings. The aim of the present work is to obtain viableprotoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patchclamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplastswith preserved membrane integrity suitable for electrophysiological experiments. We applied acustom approach for patch-clamping protoplasts using a microscope with a movable microscopestage. We recorded prominent inward and prominent outward types of membrane current profiles ofprotoplasts. Obtained data indicate that optimized isolation protocol and custom system for patchclamping, can be applied to study membrane properties of root protoplasts. PB - Belgrade : Society of Physical Chemists of Serbia C3 - Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia T1 - Protoplast patch-clamping using an upright microscope with a movable stage EP - 274 SP - 271 UR - https://hdl.handle.net/21.15107/rcub_ibiss_5508 ER -
@conference{ editor = "Čupić, Željko, Anić, Slobodan", author = "Nikolić, Ljiljana and Vidović, Marija and Todorović, Nataša and Petković, Branka and Stojadinović, Gordana and Martać, Ljiljana and Bogdanović Pristov, Jelena", year = "2022", abstract = "Investigating membrane properties of plants is a challenging task, considering that success ofexperiments is highly dependent on the possibility to isolate metabolically active protoplasts thatcan withstand membrane current recordings. The aim of the present work is to obtain viableprotoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patchclamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplastswith preserved membrane integrity suitable for electrophysiological experiments. We applied acustom approach for patch-clamping protoplasts using a microscope with a movable microscopestage. We recorded prominent inward and prominent outward types of membrane current profiles ofprotoplasts. Obtained data indicate that optimized isolation protocol and custom system for patchclamping, can be applied to study membrane properties of root protoplasts.", publisher = "Belgrade : Society of Physical Chemists of Serbia", journal = "Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia", title = "Protoplast patch-clamping using an upright microscope with a movable stage", pages = "274-271", url = "https://hdl.handle.net/21.15107/rcub_ibiss_5508" }
Čupić, Ž., Anić, S., Nikolić, L., Vidović, M., Todorović, N., Petković, B., Stojadinović, G., Martać, L.,& Bogdanović Pristov, J.. (2022). Protoplast patch-clamping using an upright microscope with a movable stage. in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia Belgrade : Society of Physical Chemists of Serbia., 271-274. https://hdl.handle.net/21.15107/rcub_ibiss_5508
Čupić Ž, Anić S, Nikolić L, Vidović M, Todorović N, Petković B, Stojadinović G, Martać L, Bogdanović Pristov J. Protoplast patch-clamping using an upright microscope with a movable stage. in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia. 2022;:271-274. https://hdl.handle.net/21.15107/rcub_ibiss_5508 .
Čupić, Željko, Anić, Slobodan, Nikolić, Ljiljana, Vidović, Marija, Todorović, Nataša, Petković, Branka, Stojadinović, Gordana, Martać, Ljiljana, Bogdanović Pristov, Jelena, "Protoplast patch-clamping using an upright microscope with a movable stage" in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia (2022):271-274, https://hdl.handle.net/21.15107/rcub_ibiss_5508 .