Extracellular vesicles from blood plasma as mediators of anti-inflammatory effects, oxidative stress and angiogenesis in HUVEC
Аутори
Zala, JanRomolo, Romolo
Matevž, Arko
Spasovski, Vesna
Iglič, Aleš
Drobne, Damjana
Kralj-Iglič, Veronika
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: Blood plasma is used in regenerative medicine for more than 30 years as it was found to have
impact on angiogenesis and proliferation of endothelial cells. This effect was initially ascribed to growth factors
and cytokines, however, particularly in the last decade researchers are focusing on platelet-derived
extracellular vesicles (PEVs) that are also present in the platelet rich plasma.
Methods: We prepared platelet and extracellular vesicles-rich plasma (PVRP) by centrifugation of the
human blood, and differential (ultra) centrifugation of PVRP to isolate PEVs. We exposed Human Umbilical Vein
Endothelial Cells (HUVEC) to 5% PEVs for 24 hours and assessed inflammation markers (Interleukin(IL)-1beta,
IL-6 and Tumor necrosis factor (TNF)-alpha using ELISA tests), oxidative stress markers (Cholinesterase
(ChE) and glutathione S-transferase (GST) activity by spectrophotometry, as well reactive oxygen species
(ROS) and lipid droplets (LD) by flow cytometry). We obser...ved morphological changes in HUVEC indicating
angiogenesis by using optical microscopy.
Results: We found that after 24 hours caused a decrease of the concentration of IL-6, IL-1beta and TNF-alpha.
Also, ChE and GST activity and ROS count were decreased. LD production, which is triggered in need to protect
the cells from free radicals and oxidative stress damage, was however higher. Treatment of the cells had
impact on cell morphology with progressed formation of the tubes and cell connecting, which is regarded as the
beginning of HUVEC angiogenesis process.
Discussion: Beneficial effect of PVRP in healing and regeneration may include suppression of inflammation
and oxidative stress by PEVs.
Извор:
Small New World 2.0, 2023, 118-118Издавач:
- Slovenian Network for Extracellular Vesicles (SiN-EV)
- Austrian Society for Extracellular Vesicles (ASEV)
- Hungarian Society for Extracellular Vesicles (HSEV)
- Slovenian Network for Extracellular Vesicles (SiN-EV)
Напомена:
- Small New World 2.0 4-5 September 2023., Graz, Austria
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Zala, Jan AU - Romolo, Romolo AU - Matevž, Arko AU - Spasovski, Vesna AU - Iglič, Aleš AU - Drobne, Damjana AU - Kralj-Iglič, Veronika PY - 2023 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2267 AB - Introduction: Blood plasma is used in regenerative medicine for more than 30 years as it was found to have impact on angiogenesis and proliferation of endothelial cells. This effect was initially ascribed to growth factors and cytokines, however, particularly in the last decade researchers are focusing on platelet-derived extracellular vesicles (PEVs) that are also present in the platelet rich plasma. Methods: We prepared platelet and extracellular vesicles-rich plasma (PVRP) by centrifugation of the human blood, and differential (ultra) centrifugation of PVRP to isolate PEVs. We exposed Human Umbilical Vein Endothelial Cells (HUVEC) to 5% PEVs for 24 hours and assessed inflammation markers (Interleukin(IL)-1beta, IL-6 and Tumor necrosis factor (TNF)-alpha using ELISA tests), oxidative stress markers (Cholinesterase (ChE) and glutathione S-transferase (GST) activity by spectrophotometry, as well reactive oxygen species (ROS) and lipid droplets (LD) by flow cytometry). We observed morphological changes in HUVEC indicating angiogenesis by using optical microscopy. Results: We found that after 24 hours caused a decrease of the concentration of IL-6, IL-1beta and TNF-alpha. Also, ChE and GST activity and ROS count were decreased. LD production, which is triggered in need to protect the cells from free radicals and oxidative stress damage, was however higher. Treatment of the cells had impact on cell morphology with progressed formation of the tubes and cell connecting, which is regarded as the beginning of HUVEC angiogenesis process. Discussion: Beneficial effect of PVRP in healing and regeneration may include suppression of inflammation and oxidative stress by PEVs. PB - Slovenian Network for Extracellular Vesicles (SiN-EV) PB - Austrian Society for Extracellular Vesicles (ASEV) PB - Hungarian Society for Extracellular Vesicles (HSEV) PB - Slovenian Network for Extracellular Vesicles (SiN-EV) C3 - Small New World 2.0 T1 - Extracellular vesicles from blood plasma as mediators of anti-inflammatory effects, oxidative stress and angiogenesis in HUVEC EP - 118 SP - 118 UR - https://hdl.handle.net/21.15107/rcub_imagine_2267 ER -
@conference{ author = "Zala, Jan and Romolo, Romolo and Matevž, Arko and Spasovski, Vesna and Iglič, Aleš and Drobne, Damjana and Kralj-Iglič, Veronika", year = "2023", abstract = "Introduction: Blood plasma is used in regenerative medicine for more than 30 years as it was found to have impact on angiogenesis and proliferation of endothelial cells. This effect was initially ascribed to growth factors and cytokines, however, particularly in the last decade researchers are focusing on platelet-derived extracellular vesicles (PEVs) that are also present in the platelet rich plasma. Methods: We prepared platelet and extracellular vesicles-rich plasma (PVRP) by centrifugation of the human blood, and differential (ultra) centrifugation of PVRP to isolate PEVs. We exposed Human Umbilical Vein Endothelial Cells (HUVEC) to 5% PEVs for 24 hours and assessed inflammation markers (Interleukin(IL)-1beta, IL-6 and Tumor necrosis factor (TNF)-alpha using ELISA tests), oxidative stress markers (Cholinesterase (ChE) and glutathione S-transferase (GST) activity by spectrophotometry, as well reactive oxygen species (ROS) and lipid droplets (LD) by flow cytometry). We observed morphological changes in HUVEC indicating angiogenesis by using optical microscopy. Results: We found that after 24 hours caused a decrease of the concentration of IL-6, IL-1beta and TNF-alpha. Also, ChE and GST activity and ROS count were decreased. LD production, which is triggered in need to protect the cells from free radicals and oxidative stress damage, was however higher. Treatment of the cells had impact on cell morphology with progressed formation of the tubes and cell connecting, which is regarded as the beginning of HUVEC angiogenesis process. Discussion: Beneficial effect of PVRP in healing and regeneration may include suppression of inflammation and oxidative stress by PEVs.", publisher = "Slovenian Network for Extracellular Vesicles (SiN-EV), Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), Slovenian Network for Extracellular Vesicles (SiN-EV)", journal = "Small New World 2.0", title = "Extracellular vesicles from blood plasma as mediators of anti-inflammatory effects, oxidative stress and angiogenesis in HUVEC", pages = "118-118", url = "https://hdl.handle.net/21.15107/rcub_imagine_2267" }
Zala, J., Romolo, R., Matevž, A., Spasovski, V., Iglič, A., Drobne, D.,& Kralj-Iglič, V.. (2023). Extracellular vesicles from blood plasma as mediators of anti-inflammatory effects, oxidative stress and angiogenesis in HUVEC. in Small New World 2.0 Slovenian Network for Extracellular Vesicles (SiN-EV)., 118-118. https://hdl.handle.net/21.15107/rcub_imagine_2267
Zala J, Romolo R, Matevž A, Spasovski V, Iglič A, Drobne D, Kralj-Iglič V. Extracellular vesicles from blood plasma as mediators of anti-inflammatory effects, oxidative stress and angiogenesis in HUVEC. in Small New World 2.0. 2023;:118-118. https://hdl.handle.net/21.15107/rcub_imagine_2267 .
Zala, Jan, Romolo, Romolo, Matevž, Arko, Spasovski, Vesna, Iglič, Aleš, Drobne, Damjana, Kralj-Iglič, Veronika, "Extracellular vesicles from blood plasma as mediators of anti-inflammatory effects, oxidative stress and angiogenesis in HUVEC" in Small New World 2.0 (2023):118-118, https://hdl.handle.net/21.15107/rcub_imagine_2267 .