Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases
Autori
Makryniotis, KonstantinosNikolaivits, Efstratios
Taxeidis, George
Nikodinović-Runić, Jasmina
Topakas, Evangelos
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation abi...lity of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.
Ključne reči:
1JFR / bacterial hydrolases / IsPETase / plastic pollution / polyester degradationIzvor:
Biotechnology Journal, 2024, n/a, n/a, 2400053-Finansiranje / projekti:
- European Union’s Horizon 2020 research and innovation programme under grant agreement No 870292 (BioICEP Project)
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.202400053https://imagine.imgge.bg.ac.rs/handle/123456789/2341
Kolekcije
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Makryniotis, Konstantinos AU - Nikolaivits, Efstratios AU - Taxeidis, George AU - Nikodinović-Runić, Jasmina AU - Topakas, Evangelos PY - 2024 UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.202400053 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2341 AB - The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation. T2 - Biotechnology Journal T2 - Biotechnology Journal T1 - Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases IS - n/a SP - 2400053 VL - n/a DO - 10.1002/biot.202400053 ER -
@article{ author = "Makryniotis, Konstantinos and Nikolaivits, Efstratios and Taxeidis, George and Nikodinović-Runić, Jasmina and Topakas, Evangelos", year = "2024", abstract = "The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.", journal = "Biotechnology Journal, Biotechnology Journal", title = "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases", number = "n/a", pages = "2400053", volume = "n/a", doi = "10.1002/biot.202400053" }
Makryniotis, K., Nikolaivits, E., Taxeidis, G., Nikodinović-Runić, J.,& Topakas, E.. (2024). Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal, n/a(n/a), 2400053. https://doi.org/10.1002/biot.202400053
Makryniotis K, Nikolaivits E, Taxeidis G, Nikodinović-Runić J, Topakas E. Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal. 2024;n/a(n/a):2400053. doi:10.1002/biot.202400053 .
Makryniotis, Konstantinos, Nikolaivits, Efstratios, Taxeidis, George, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases" in Biotechnology Journal, n/a, no. n/a (2024):2400053, https://doi.org/10.1002/biot.202400053 . .