THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS
Autori
Golić, NatašaTerzić Vidojević, Amarela
Tolinački, Maja
Dinić, Miroslav
Đokić, Jelena
Todorović Vukotić, Nevena
Lukić, Jovanka
Živković, Milica
Nastasijević, Branislav
Soković, Svetlana
Brdarić, Emilija
Radojević, Dušan
Ostala autorstva
Dimkić, IvicaKekić, Dušan
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
There has been an epidemic of various non-communicable
degenerative and autoimmune diseases,
strongly associated with the modern
lifestyle. Among them, neurodegenerative and
psychiatric disorders represent a huge burden on
society. Recently, all these diseases have been associated
with the gut microbiota dysbiosis. Gut
microbiota-host interaction research has been
greatly improved due to development of molecular
high-throughput techniques based on
various ‘omics’ techniques coupled with bioinformatics
and data science developments. However,
the mechanisms of the host–microbiota crosstalk
are still poorly understood. The NextGenBiotics
project proposes an innovative integrative
multi-omics research strategy for deciphering
the mechanism behind the cross-talk among
microbiota and gut-brain-axis. The 118 novel
NGPs candidates belonging to Dorea sp., Blautia
sp., Bacteroides sp., Roseburia sp., Sellimonas
sp., Faecalicatena sp., Phascolarctobacterium faecium,
and Faeca...limonas sp. were cultivated. The
25 NGPs with confirmed safe status and potential
probiotic potential were screened in C. elegans
model for their effects on behavioural and neuronal
activity. The most prominent candidates
with ability to upregulate expression of genes
involved in neurotransmiting are further tested
in EAE (an animal model for MS) and CUMS depression
model. The specific microbiota-derived
metabolites have been identified as potential
neuro- and psycho-biotics. The NextGenBiotics is
highly ambitious project, dedicated to pioneering
work in the field of multi-omics studies related
to the cultivation of novel anaerobic NGPs
and the studying of their effect on MGBA. This
concept enabled studying bidirectional communication
between gut microbiota and brain
on the functional level that will significantly
contribute to the growing body data related to
MGBA. The results obtained during NextGenBiotics
determined the genes/metabolites and the
associated mechanisms involved in health-promoting
effects of NGPs in MGBA beyond stateof-
the-art, broadening the scientific knowledge
and opening up the possible novel therapeutic
approaches in prevention and therapy of neurodegenerative
and psychiatric diseases.
Ključne reči:
next generation probiotics / microbiota-gut-brain axis / neurobiotics / psyhobioticsIzvor:
XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health, 2024, 106-106Izdavač:
- Serbian Society for Microbiology
Finansiranje / projekti:
- NextGenBiotics - The use of integrative multi-omics approach in cultivation and characterization of gut bacteria related to microbiota-gut-brain axis as a source for Next Generation Probiotics (RS-ScienceFundRS-Ideje-7744507)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
- VINS
Napomena:
- Book of abstract: From biotechnology to human and planetary health XIII congress of microbiologists of Serbia with international participation Mikromed regio 5, ums series 24: 4th – 6th april 2024, Mona Plaza hotel, Belgrade, Serbia
Kolekcije
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Golić, Nataša AU - Terzić Vidojević, Amarela AU - Tolinački, Maja AU - Dinić, Miroslav AU - Đokić, Jelena AU - Todorović Vukotić, Nevena AU - Lukić, Jovanka AU - Živković, Milica AU - Nastasijević, Branislav AU - Soković, Svetlana AU - Brdarić, Emilija AU - Radojević, Dušan PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2372 AB - There has been an epidemic of various non-communicable degenerative and autoimmune diseases, strongly associated with the modern lifestyle. Among them, neurodegenerative and psychiatric disorders represent a huge burden on society. Recently, all these diseases have been associated with the gut microbiota dysbiosis. Gut microbiota-host interaction research has been greatly improved due to development of molecular high-throughput techniques based on various ‘omics’ techniques coupled with bioinformatics and data science developments. However, the mechanisms of the host–microbiota crosstalk are still poorly understood. The NextGenBiotics project proposes an innovative integrative multi-omics research strategy for deciphering the mechanism behind the cross-talk among microbiota and gut-brain-axis. The 118 novel NGPs candidates belonging to Dorea sp., Blautia sp., Bacteroides sp., Roseburia sp., Sellimonas sp., Faecalicatena sp., Phascolarctobacterium faecium, and Faecalimonas sp. were cultivated. The 25 NGPs with confirmed safe status and potential probiotic potential were screened in C. elegans model for their effects on behavioural and neuronal activity. The most prominent candidates with ability to upregulate expression of genes involved in neurotransmiting are further tested in EAE (an animal model for MS) and CUMS depression model. The specific microbiota-derived metabolites have been identified as potential neuro- and psycho-biotics. The NextGenBiotics is highly ambitious project, dedicated to pioneering work in the field of multi-omics studies related to the cultivation of novel anaerobic NGPs and the studying of their effect on MGBA. This concept enabled studying bidirectional communication between gut microbiota and brain on the functional level that will significantly contribute to the growing body data related to MGBA. The results obtained during NextGenBiotics determined the genes/metabolites and the associated mechanisms involved in health-promoting effects of NGPs in MGBA beyond stateof- the-art, broadening the scientific knowledge and opening up the possible novel therapeutic approaches in prevention and therapy of neurodegenerative and psychiatric diseases. PB - Serbian Society for Microbiology C3 - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health T1 - THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS EP - 106 SP - 106 UR - https://hdl.handle.net/21.15107/rcub_imagine_2372 ER -
@conference{ author = "Golić, Nataša and Terzić Vidojević, Amarela and Tolinački, Maja and Dinić, Miroslav and Đokić, Jelena and Todorović Vukotić, Nevena and Lukić, Jovanka and Živković, Milica and Nastasijević, Branislav and Soković, Svetlana and Brdarić, Emilija and Radojević, Dušan", year = "2024", abstract = "There has been an epidemic of various non-communicable degenerative and autoimmune diseases, strongly associated with the modern lifestyle. Among them, neurodegenerative and psychiatric disorders represent a huge burden on society. Recently, all these diseases have been associated with the gut microbiota dysbiosis. Gut microbiota-host interaction research has been greatly improved due to development of molecular high-throughput techniques based on various ‘omics’ techniques coupled with bioinformatics and data science developments. However, the mechanisms of the host–microbiota crosstalk are still poorly understood. The NextGenBiotics project proposes an innovative integrative multi-omics research strategy for deciphering the mechanism behind the cross-talk among microbiota and gut-brain-axis. The 118 novel NGPs candidates belonging to Dorea sp., Blautia sp., Bacteroides sp., Roseburia sp., Sellimonas sp., Faecalicatena sp., Phascolarctobacterium faecium, and Faecalimonas sp. were cultivated. The 25 NGPs with confirmed safe status and potential probiotic potential were screened in C. elegans model for their effects on behavioural and neuronal activity. The most prominent candidates with ability to upregulate expression of genes involved in neurotransmiting are further tested in EAE (an animal model for MS) and CUMS depression model. The specific microbiota-derived metabolites have been identified as potential neuro- and psycho-biotics. The NextGenBiotics is highly ambitious project, dedicated to pioneering work in the field of multi-omics studies related to the cultivation of novel anaerobic NGPs and the studying of their effect on MGBA. This concept enabled studying bidirectional communication between gut microbiota and brain on the functional level that will significantly contribute to the growing body data related to MGBA. The results obtained during NextGenBiotics determined the genes/metabolites and the associated mechanisms involved in health-promoting effects of NGPs in MGBA beyond stateof- the-art, broadening the scientific knowledge and opening up the possible novel therapeutic approaches in prevention and therapy of neurodegenerative and psychiatric diseases.", publisher = "Serbian Society for Microbiology", journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health", title = "THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS", pages = "106-106", url = "https://hdl.handle.net/21.15107/rcub_imagine_2372" }
Golić, N., Terzić Vidojević, A., Tolinački, M., Dinić, M., Đokić, J., Todorović Vukotić, N., Lukić, J., Živković, M., Nastasijević, B., Soković, S., Brdarić, E.,& Radojević, D.. (2024). THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health Serbian Society for Microbiology., 106-106. https://hdl.handle.net/21.15107/rcub_imagine_2372
Golić N, Terzić Vidojević A, Tolinački M, Dinić M, Đokić J, Todorović Vukotić N, Lukić J, Živković M, Nastasijević B, Soković S, Brdarić E, Radojević D. THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:106-106. https://hdl.handle.net/21.15107/rcub_imagine_2372 .
Golić, Nataša, Terzić Vidojević, Amarela, Tolinački, Maja, Dinić, Miroslav, Đokić, Jelena, Todorović Vukotić, Nevena, Lukić, Jovanka, Živković, Milica, Nastasijević, Branislav, Soković, Svetlana, Brdarić, Emilija, Radojević, Dušan, "THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):106-106, https://hdl.handle.net/21.15107/rcub_imagine_2372 .