BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE
Authors
Vukotić, GoranObradović, Mina
Plačkić, Nikola
Kljajević, Nemanja
Pavić, Aleksandar
Kekić, Dušan
Gajić, Ina
Kojić, Milan
Stanisavljević, Nemanja
Contributors
Dimkić, IvicaKekić, Dušan
Conference object (Published version)
Metadata
Show full item recordAbstract
Antimicrobial resistance (AMR) arises when
bacteria and other microbes stop responding
to medications. AMR is now recognized as one
of serious global health threats, repeatedly
appearing in the World Health Organization’s
(WHO) lists of urgent global health challenges,
including the 2024 list. It is taking a fatal toll
– nearly 5 million deaths globally per year are
associated with AMR, encompassing 1.27 million
directly attributed to AMR. The COVID-19
pandemic paved the way for aggravation of
bacterial AMR – primarily due to enhancement
in unspecific and unjustified prescription and
use of broad-spectrum antibiotics, resulting in
what is now recognized as „silent pandemic of
AMR“. Bacteriophages (phages) are natural and
specific predators of bacteria - viruses that can
infect, replicate inside and lyse arguably any
bacteria. Their therapeutic potential is being
hastily evaluated through different approaches:
in silico, in vitro, ex vivo and in vivo – in laboratory
...
animals as well as in human case and clinical
studies. Although the results are promising,bacteria rapidly develop resistance against
phages, which why the isolation and research
of new phages is needed. Our work is concentrated
on three bacterial species for which critical
priority by WHO has been declared – carbapenem-
resistant Acinetobacter baumannii,
Pseudomonas aeruginosa and Klebsiella pneumoniae.
Twenty distinct pathogenic strains of
A. baumannii, 6 K. pneumoniae and 6 P. aeruginosa
were used as targets for bacteriophage
isolation, and total of 14, 22 and 8 potentially
distinct phages were collected, respectively. All
strains were nosocomial isolates obtained from
various tissues, including from terminally ill patients.
Six phages were characterized in detail.
In particular, phage vB_AbaM_ISTD was applied
against A. baumannii in zebrafish embryo
model of systemic infection, and demonstrated
powerful therapeutic potential, eradicating the
infection. Interestingly, its DNA was characterized
with highly modified thymidine (amassing
1228 Da), making it the largest non-canonical
deoxynucleoside reported so far.
Keywords:
antimicrobial resistance (AMR) / nosocomial infections / bacteriophages / zebrafish modelSource:
XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health, 2024, 121-121Publisher:
- Serbian Society for Microbiology
Note:
- Book of abstract: From biotechnology to human and planetary health XIII congress of microbiologists of Serbia with international participation Mikromed regio 5, ums series 24: 4th – 6th april 2024, Mona Plaza hotel, Belgrade, Serbia
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Vukotić, Goran AU - Obradović, Mina AU - Plačkić, Nikola AU - Kljajević, Nemanja AU - Pavić, Aleksandar AU - Kekić, Dušan AU - Gajić, Ina AU - Kojić, Milan AU - Stanisavljević, Nemanja PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2375 AB - Antimicrobial resistance (AMR) arises when bacteria and other microbes stop responding to medications. AMR is now recognized as one of serious global health threats, repeatedly appearing in the World Health Organization’s (WHO) lists of urgent global health challenges, including the 2024 list. It is taking a fatal toll – nearly 5 million deaths globally per year are associated with AMR, encompassing 1.27 million directly attributed to AMR. The COVID-19 pandemic paved the way for aggravation of bacterial AMR – primarily due to enhancement in unspecific and unjustified prescription and use of broad-spectrum antibiotics, resulting in what is now recognized as „silent pandemic of AMR“. Bacteriophages (phages) are natural and specific predators of bacteria - viruses that can infect, replicate inside and lyse arguably any bacteria. Their therapeutic potential is being hastily evaluated through different approaches: in silico, in vitro, ex vivo and in vivo – in laboratory animals as well as in human case and clinical studies. Although the results are promising,bacteria rapidly develop resistance against phages, which why the isolation and research of new phages is needed. Our work is concentrated on three bacterial species for which critical priority by WHO has been declared – carbapenem- resistant Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae. Twenty distinct pathogenic strains of A. baumannii, 6 K. pneumoniae and 6 P. aeruginosa were used as targets for bacteriophage isolation, and total of 14, 22 and 8 potentially distinct phages were collected, respectively. All strains were nosocomial isolates obtained from various tissues, including from terminally ill patients. Six phages were characterized in detail. In particular, phage vB_AbaM_ISTD was applied against A. baumannii in zebrafish embryo model of systemic infection, and demonstrated powerful therapeutic potential, eradicating the infection. Interestingly, its DNA was characterized with highly modified thymidine (amassing 1228 Da), making it the largest non-canonical deoxynucleoside reported so far. PB - Serbian Society for Microbiology C3 - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health T1 - BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE EP - 121 SP - 121 UR - https://hdl.handle.net/21.15107/rcub_imagine_2375 ER -
@conference{ author = "Vukotić, Goran and Obradović, Mina and Plačkić, Nikola and Kljajević, Nemanja and Pavić, Aleksandar and Kekić, Dušan and Gajić, Ina and Kojić, Milan and Stanisavljević, Nemanja", year = "2024", abstract = "Antimicrobial resistance (AMR) arises when bacteria and other microbes stop responding to medications. AMR is now recognized as one of serious global health threats, repeatedly appearing in the World Health Organization’s (WHO) lists of urgent global health challenges, including the 2024 list. It is taking a fatal toll – nearly 5 million deaths globally per year are associated with AMR, encompassing 1.27 million directly attributed to AMR. The COVID-19 pandemic paved the way for aggravation of bacterial AMR – primarily due to enhancement in unspecific and unjustified prescription and use of broad-spectrum antibiotics, resulting in what is now recognized as „silent pandemic of AMR“. Bacteriophages (phages) are natural and specific predators of bacteria - viruses that can infect, replicate inside and lyse arguably any bacteria. Their therapeutic potential is being hastily evaluated through different approaches: in silico, in vitro, ex vivo and in vivo – in laboratory animals as well as in human case and clinical studies. Although the results are promising,bacteria rapidly develop resistance against phages, which why the isolation and research of new phages is needed. Our work is concentrated on three bacterial species for which critical priority by WHO has been declared – carbapenem- resistant Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae. Twenty distinct pathogenic strains of A. baumannii, 6 K. pneumoniae and 6 P. aeruginosa were used as targets for bacteriophage isolation, and total of 14, 22 and 8 potentially distinct phages were collected, respectively. All strains were nosocomial isolates obtained from various tissues, including from terminally ill patients. Six phages were characterized in detail. In particular, phage vB_AbaM_ISTD was applied against A. baumannii in zebrafish embryo model of systemic infection, and demonstrated powerful therapeutic potential, eradicating the infection. Interestingly, its DNA was characterized with highly modified thymidine (amassing 1228 Da), making it the largest non-canonical deoxynucleoside reported so far.", publisher = "Serbian Society for Microbiology", journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health", title = "BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE", pages = "121-121", url = "https://hdl.handle.net/21.15107/rcub_imagine_2375" }
Vukotić, G., Obradović, M., Plačkić, N., Kljajević, N., Pavić, A., Kekić, D., Gajić, I., Kojić, M.,& Stanisavljević, N.. (2024). BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health Serbian Society for Microbiology., 121-121. https://hdl.handle.net/21.15107/rcub_imagine_2375
Vukotić G, Obradović M, Plačkić N, Kljajević N, Pavić A, Kekić D, Gajić I, Kojić M, Stanisavljević N. BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:121-121. https://hdl.handle.net/21.15107/rcub_imagine_2375 .
Vukotić, Goran, Obradović, Mina, Plačkić, Nikola, Kljajević, Nemanja, Pavić, Aleksandar, Kekić, Dušan, Gajić, Ina, Kojić, Milan, Stanisavljević, Nemanja, "BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):121-121, https://hdl.handle.net/21.15107/rcub_imagine_2375 .