NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS
2024
Preuzimanje 🢃
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The rapid emergence and spread of multidrug-
resistant pathogens present a global
healthcare challenge. One common cause of
resistance and/or tolerance to antibiotics is
biofilms, a complex communities of bacteria
embedded in a self-produced matrix. Biofilm
formation and maturation are regulated by
quorum sensing, a cell density-dependent communication
system that relies on the synthesis,
diffusion, and detection of small signaling molecules
- autoinducers (AIs). Quorum quenching
(QQ) enzymes that cut Ais emerged as a promising
strategy for persistent bacterial infections.
However, a significant drawback for the use of
QQ enzymes as therapeutics is their poor stability
and efficacy in vivo. Since one of the major
health issues linked to biofilm development is
persistent wound infections, our goal was to
improve enzyme properties by immobilizing it
on a natural biopolymer to make it suitable for
use as a wound dressing. The best candidate for immobilization was YtnP ...lactonase from Bacillus
paralicheniformis ZP1, as in concentrations
higher than 25 μg/mL it improved the survival of
Pseudomonas aeruginosa PAO1-infected zebrafish,
rescuing 80% of embryos. When combined
with tobramycin or gentamicin, the survival
rate of zebrafish embryos increased to 100%.
Purified YtnP lactonase at a concentration of 1
mg was immobilized on 10 mg of polymer disks
by crosslinking with glutaraldehyde. Specific
modifications of the polymer were also made to
eliminate the use of glutaraldehyde, which is a
skin irritant. In in vivo experiments on a murine
chronic wound model, immobilized enzyme
inhibited biofilm development, cleared already
formed biofilms, and overall improved wound
healing. These results provide a foundation for
the development of advanced wound dressings
that will prevent infection development in
wounds and enable proper therapy for infected
chronic wounds.
Ključne reči:
biofilm / chronic wounds / Pseudomonas aeruginosa PAO1 / YtnP lactonase / immobilizationIzvor:
XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health, 2024, 126-126Izdavač:
- Serbian Society for Microbiology
Napomena:
- Book of abstract: From biotechnology to human and planetary health XIII congress of microbiologists of Serbia with international participation Mikromed regio 5, ums series 24: 4th – 6th april 2024, Mona Plaza hotel, Belgrade, Serbia
Kolekcije
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Đokić, Lidija AU - Rokić, Miloš AU - Šenerović, Lidija PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2376 AB - The rapid emergence and spread of multidrug- resistant pathogens present a global healthcare challenge. One common cause of resistance and/or tolerance to antibiotics is biofilms, a complex communities of bacteria embedded in a self-produced matrix. Biofilm formation and maturation are regulated by quorum sensing, a cell density-dependent communication system that relies on the synthesis, diffusion, and detection of small signaling molecules - autoinducers (AIs). Quorum quenching (QQ) enzymes that cut Ais emerged as a promising strategy for persistent bacterial infections. However, a significant drawback for the use of QQ enzymes as therapeutics is their poor stability and efficacy in vivo. Since one of the major health issues linked to biofilm development is persistent wound infections, our goal was to improve enzyme properties by immobilizing it on a natural biopolymer to make it suitable for use as a wound dressing. The best candidate for immobilization was YtnP lactonase from Bacillus paralicheniformis ZP1, as in concentrations higher than 25 μg/mL it improved the survival of Pseudomonas aeruginosa PAO1-infected zebrafish, rescuing 80% of embryos. When combined with tobramycin or gentamicin, the survival rate of zebrafish embryos increased to 100%. Purified YtnP lactonase at a concentration of 1 mg was immobilized on 10 mg of polymer disks by crosslinking with glutaraldehyde. Specific modifications of the polymer were also made to eliminate the use of glutaraldehyde, which is a skin irritant. In in vivo experiments on a murine chronic wound model, immobilized enzyme inhibited biofilm development, cleared already formed biofilms, and overall improved wound healing. These results provide a foundation for the development of advanced wound dressings that will prevent infection development in wounds and enable proper therapy for infected chronic wounds. PB - Serbian Society for Microbiology C3 - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health T1 - NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS EP - 126 SP - 126 UR - https://hdl.handle.net/21.15107/rcub_imagine_2376 ER -
@conference{ author = "Đokić, Lidija and Rokić, Miloš and Šenerović, Lidija", year = "2024", abstract = "The rapid emergence and spread of multidrug- resistant pathogens present a global healthcare challenge. One common cause of resistance and/or tolerance to antibiotics is biofilms, a complex communities of bacteria embedded in a self-produced matrix. Biofilm formation and maturation are regulated by quorum sensing, a cell density-dependent communication system that relies on the synthesis, diffusion, and detection of small signaling molecules - autoinducers (AIs). Quorum quenching (QQ) enzymes that cut Ais emerged as a promising strategy for persistent bacterial infections. However, a significant drawback for the use of QQ enzymes as therapeutics is their poor stability and efficacy in vivo. Since one of the major health issues linked to biofilm development is persistent wound infections, our goal was to improve enzyme properties by immobilizing it on a natural biopolymer to make it suitable for use as a wound dressing. The best candidate for immobilization was YtnP lactonase from Bacillus paralicheniformis ZP1, as in concentrations higher than 25 μg/mL it improved the survival of Pseudomonas aeruginosa PAO1-infected zebrafish, rescuing 80% of embryos. When combined with tobramycin or gentamicin, the survival rate of zebrafish embryos increased to 100%. Purified YtnP lactonase at a concentration of 1 mg was immobilized on 10 mg of polymer disks by crosslinking with glutaraldehyde. Specific modifications of the polymer were also made to eliminate the use of glutaraldehyde, which is a skin irritant. In in vivo experiments on a murine chronic wound model, immobilized enzyme inhibited biofilm development, cleared already formed biofilms, and overall improved wound healing. These results provide a foundation for the development of advanced wound dressings that will prevent infection development in wounds and enable proper therapy for infected chronic wounds.", publisher = "Serbian Society for Microbiology", journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health", title = "NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS", pages = "126-126", url = "https://hdl.handle.net/21.15107/rcub_imagine_2376" }
Đokić, L., Rokić, M.,& Šenerović, L.. (2024). NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health Serbian Society for Microbiology., 126-126. https://hdl.handle.net/21.15107/rcub_imagine_2376
Đokić L, Rokić M, Šenerović L. NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:126-126. https://hdl.handle.net/21.15107/rcub_imagine_2376 .
Đokić, Lidija, Rokić, Miloš, Šenerović, Lidija, "NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):126-126, https://hdl.handle.net/21.15107/rcub_imagine_2376 .