HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING
2024
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Аутори
Dinić, MiroslavL. Burgess, Jamie
Lukić, Jovanka
Catanuto, Paola
Radojević, Dušan
Marjanović, Jelena
Verpile, Rebecca
R. Thaller, Seth
Gonzalez, Tammy
Golić, Nataša
Tomić- Canić, Marjana
Strahinić, Ivana
Pastar, Irena
Остала ауторства
Dimkić, IvicaKekić, Dušan
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Skin microbiome emerged as an important
factor which can balance tissue repair process
and wound healing. Recent evidence suggest
that intracellular bacterial localization could be
associated with the aberrant healing observed
in patients with chronic wounds, while therapeutics
targeting intracellular bacteria remain
limited. Probiotic lactobacilli and their bioactive
lysates (postbiotics) are well known for their role
in maintenance of gut epithelial homeostasis.
Hence, in this study we focused to understand
the mechanisms of cutaneous response to fourteen
postbiotics derived from different lactobacilli
to reduce intracellular Staphylococcus aureus
colonization and promote healing. Latilactobacillus
curvatus BGMK2-41 demonstrated the
most efficient capability to reduce intracellular infection by S. aureus in keratinocytes in vitro and
infection of human skin explants. Reduction of
bacterial number was followed by upregulation
of the expression of antimicrobial respo...nse
genes. Furthermore, BGMK2-41 postbiotic treatment
stimulates keratinocyte migration in vitro
and increases expression of anti-inflammatory
cytokine IL-10, promotes wound closure and
strengthens the epidermal barrier via upregulation
of tight junction proteins in a human ex vivo
wound model. Altogether, this study provided
evidence that postbiotics could stimulate fortification
of epithelial barrier to suppress dissemination
of intracellular pathogens which can be
used as a novel approach to treat dermatologic
and wound healing disorders associated with
persistent infections.
Кључне речи:
lactobacilli / postbiotics / Staphylococcus aureus / wound healing / skin barrierИзвор:
XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health, 2024, 133-133Издавач:
- Serbian Society for Microbiology
Финансирање / пројекти:
- This work was supported by NIH 1R01DK136241 grant, Science Fund of the Republic of Serbia, Diaspora Collaboration Program grant (PLASH, 6426409; MD, IP and IS)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Напомена:
- Book of abstract: From biotechnology to human and planetary health XIII congress of microbiologists of Serbia with international participation Mikromed regio 5, ums series 24: 4th – 6th april 2024, Mona Plaza hotel, Belgrade, Serbia
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Dinić, Miroslav AU - L. Burgess, Jamie AU - Lukić, Jovanka AU - Catanuto, Paola AU - Radojević, Dušan AU - Marjanović, Jelena AU - Verpile, Rebecca AU - R. Thaller, Seth AU - Gonzalez, Tammy AU - Golić, Nataša AU - Tomić- Canić, Marjana AU - Strahinić, Ivana AU - Pastar, Irena PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2378 AB - Skin microbiome emerged as an important factor which can balance tissue repair process and wound healing. Recent evidence suggest that intracellular bacterial localization could be associated with the aberrant healing observed in patients with chronic wounds, while therapeutics targeting intracellular bacteria remain limited. Probiotic lactobacilli and their bioactive lysates (postbiotics) are well known for their role in maintenance of gut epithelial homeostasis. Hence, in this study we focused to understand the mechanisms of cutaneous response to fourteen postbiotics derived from different lactobacilli to reduce intracellular Staphylococcus aureus colonization and promote healing. Latilactobacillus curvatus BGMK2-41 demonstrated the most efficient capability to reduce intracellular infection by S. aureus in keratinocytes in vitro and infection of human skin explants. Reduction of bacterial number was followed by upregulation of the expression of antimicrobial response genes. Furthermore, BGMK2-41 postbiotic treatment stimulates keratinocyte migration in vitro and increases expression of anti-inflammatory cytokine IL-10, promotes wound closure and strengthens the epidermal barrier via upregulation of tight junction proteins in a human ex vivo wound model. Altogether, this study provided evidence that postbiotics could stimulate fortification of epithelial barrier to suppress dissemination of intracellular pathogens which can be used as a novel approach to treat dermatologic and wound healing disorders associated with persistent infections. PB - Serbian Society for Microbiology C3 - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health T1 - HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING EP - 133 SP - 133 UR - https://hdl.handle.net/21.15107/rcub_imagine_2378 ER -
@conference{ author = "Dinić, Miroslav and L. Burgess, Jamie and Lukić, Jovanka and Catanuto, Paola and Radojević, Dušan and Marjanović, Jelena and Verpile, Rebecca and R. Thaller, Seth and Gonzalez, Tammy and Golić, Nataša and Tomić- Canić, Marjana and Strahinić, Ivana and Pastar, Irena", year = "2024", abstract = "Skin microbiome emerged as an important factor which can balance tissue repair process and wound healing. Recent evidence suggest that intracellular bacterial localization could be associated with the aberrant healing observed in patients with chronic wounds, while therapeutics targeting intracellular bacteria remain limited. Probiotic lactobacilli and their bioactive lysates (postbiotics) are well known for their role in maintenance of gut epithelial homeostasis. Hence, in this study we focused to understand the mechanisms of cutaneous response to fourteen postbiotics derived from different lactobacilli to reduce intracellular Staphylococcus aureus colonization and promote healing. Latilactobacillus curvatus BGMK2-41 demonstrated the most efficient capability to reduce intracellular infection by S. aureus in keratinocytes in vitro and infection of human skin explants. Reduction of bacterial number was followed by upregulation of the expression of antimicrobial response genes. Furthermore, BGMK2-41 postbiotic treatment stimulates keratinocyte migration in vitro and increases expression of anti-inflammatory cytokine IL-10, promotes wound closure and strengthens the epidermal barrier via upregulation of tight junction proteins in a human ex vivo wound model. Altogether, this study provided evidence that postbiotics could stimulate fortification of epithelial barrier to suppress dissemination of intracellular pathogens which can be used as a novel approach to treat dermatologic and wound healing disorders associated with persistent infections.", publisher = "Serbian Society for Microbiology", journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health", title = "HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING", pages = "133-133", url = "https://hdl.handle.net/21.15107/rcub_imagine_2378" }
Dinić, M., L. Burgess, J., Lukić, J., Catanuto, P., Radojević, D., Marjanović, J., Verpile, R., R. Thaller, S., Gonzalez, T., Golić, N., Tomić- Canić, M., Strahinić, I.,& Pastar, I.. (2024). HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health Serbian Society for Microbiology., 133-133. https://hdl.handle.net/21.15107/rcub_imagine_2378
Dinić M, L. Burgess J, Lukić J, Catanuto P, Radojević D, Marjanović J, Verpile R, R. Thaller S, Gonzalez T, Golić N, Tomić- Canić M, Strahinić I, Pastar I. HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:133-133. https://hdl.handle.net/21.15107/rcub_imagine_2378 .
Dinić, Miroslav, L. Burgess, Jamie, Lukić, Jovanka, Catanuto, Paola, Radojević, Dušan, Marjanović, Jelena, Verpile, Rebecca, R. Thaller, Seth, Gonzalez, Tammy, Golić, Nataša, Tomić- Canić, Marjana, Strahinić, Ivana, Pastar, Irena, "HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):133-133, https://hdl.handle.net/21.15107/rcub_imagine_2378 .