CHEMICAL COMPOSITION AND QUORUM SENSING INHIBITION ACTIVITY OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS
Authors
Stanisavljević, NemanjaMalešević, Milka
Ćurčić, Jovana
Matijašević, Danka
Kostić, Aleksandar
Milinčić, Danijel
Gašić, Uroš
Pešić, Mirjana
Contributors
Dimkić, IvicaKekić, Dušan
Conference object (Published version)
Metadata
Show full item recordAbstract
During the past decades several quorum sensing
inhibitors (QSI) of plant origin have been isolated
and chemically characterized. QSI agents
of plant origin represent potential alternative
or complementary approach to antibiotic treatment
of multidrug-resistant bacteria and infections
caused by bacterial biofilms. The aim of
the current study was to screen QSI activities
of horseradish root extracts obtained using
different organic solvents and different root
processing methods (drying at 40°C, 60°C or extraction
of fresh material). Common opportunistic
pathogen Pseudomonas aeruginosa MMA83
was used for QSI screen. RT-qPCR was used to
analyze the effect of the extract on the relative
mRNA levels of the genes QS (lasR, lasI, rhlR, rhlI,
mvfR, pqsH) and the genes involved in P. aeruginosa
MMA83 virulence (lasB, phzM, rhlC, algK,
pvdS). Chemical composition of extracts was
determined by UHPLC Q-ToF MS analysis. The
most active extract obtained using fresh roots
and hex...ane/ethyl acetate (1:1) solvent mixture
was able to significantly reduce content all examined
mRNA. Qualitative chemical analysis
reviled presence of 15 phenolic acids and their
derivatives, 9 flavonoids and 10 glucosinolates
in majority of examined extracts. It is significant
to emphasize that the most active QSI extract
did not contain a single one, out of ten dominant
glucosinolates, which have undergone to
hydrolysis yielding isothiocyanates and other
sulphur-containing compounds responsible for
QSI effects. Our results strongly indicate that
even mild thermal treatment (40°C) of horseradish
roots prior to extraction could lead to severe
reduction or loss of QSI activity.
Keywords:
horseradish / Pseudomonas aeruginosa / quorum sensing inhibition / UHPLC Q-ToF MSSource:
XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health, 2024, 147-147Publisher:
- Serbian Society for Microbiology
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
Note:
- Book of abstract: From biotechnology to human and planetary health XIII congress of microbiologists of Serbia with international participation Mikromed regio 5, ums series 24: 4th – 6th april 2024, Mona Plaza hotel, Belgrade, Serbia
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Stanisavljević, Nemanja AU - Malešević, Milka AU - Ćurčić, Jovana AU - Matijašević, Danka AU - Kostić, Aleksandar AU - Milinčić, Danijel AU - Gašić, Uroš AU - Pešić, Mirjana PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2382 AB - During the past decades several quorum sensing inhibitors (QSI) of plant origin have been isolated and chemically characterized. QSI agents of plant origin represent potential alternative or complementary approach to antibiotic treatment of multidrug-resistant bacteria and infections caused by bacterial biofilms. The aim of the current study was to screen QSI activities of horseradish root extracts obtained using different organic solvents and different root processing methods (drying at 40°C, 60°C or extraction of fresh material). Common opportunistic pathogen Pseudomonas aeruginosa MMA83 was used for QSI screen. RT-qPCR was used to analyze the effect of the extract on the relative mRNA levels of the genes QS (lasR, lasI, rhlR, rhlI, mvfR, pqsH) and the genes involved in P. aeruginosa MMA83 virulence (lasB, phzM, rhlC, algK, pvdS). Chemical composition of extracts was determined by UHPLC Q-ToF MS analysis. The most active extract obtained using fresh roots and hexane/ethyl acetate (1:1) solvent mixture was able to significantly reduce content all examined mRNA. Qualitative chemical analysis reviled presence of 15 phenolic acids and their derivatives, 9 flavonoids and 10 glucosinolates in majority of examined extracts. It is significant to emphasize that the most active QSI extract did not contain a single one, out of ten dominant glucosinolates, which have undergone to hydrolysis yielding isothiocyanates and other sulphur-containing compounds responsible for QSI effects. Our results strongly indicate that even mild thermal treatment (40°C) of horseradish roots prior to extraction could lead to severe reduction or loss of QSI activity. PB - Serbian Society for Microbiology C3 - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health T1 - CHEMICAL COMPOSITION AND QUORUM SENSING INHIBITION ACTIVITY OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS EP - 147 SP - 147 UR - https://hdl.handle.net/21.15107/rcub_imagine_2382 ER -
@conference{ author = "Stanisavljević, Nemanja and Malešević, Milka and Ćurčić, Jovana and Matijašević, Danka and Kostić, Aleksandar and Milinčić, Danijel and Gašić, Uroš and Pešić, Mirjana", year = "2024", abstract = "During the past decades several quorum sensing inhibitors (QSI) of plant origin have been isolated and chemically characterized. QSI agents of plant origin represent potential alternative or complementary approach to antibiotic treatment of multidrug-resistant bacteria and infections caused by bacterial biofilms. The aim of the current study was to screen QSI activities of horseradish root extracts obtained using different organic solvents and different root processing methods (drying at 40°C, 60°C or extraction of fresh material). Common opportunistic pathogen Pseudomonas aeruginosa MMA83 was used for QSI screen. RT-qPCR was used to analyze the effect of the extract on the relative mRNA levels of the genes QS (lasR, lasI, rhlR, rhlI, mvfR, pqsH) and the genes involved in P. aeruginosa MMA83 virulence (lasB, phzM, rhlC, algK, pvdS). Chemical composition of extracts was determined by UHPLC Q-ToF MS analysis. The most active extract obtained using fresh roots and hexane/ethyl acetate (1:1) solvent mixture was able to significantly reduce content all examined mRNA. Qualitative chemical analysis reviled presence of 15 phenolic acids and their derivatives, 9 flavonoids and 10 glucosinolates in majority of examined extracts. It is significant to emphasize that the most active QSI extract did not contain a single one, out of ten dominant glucosinolates, which have undergone to hydrolysis yielding isothiocyanates and other sulphur-containing compounds responsible for QSI effects. Our results strongly indicate that even mild thermal treatment (40°C) of horseradish roots prior to extraction could lead to severe reduction or loss of QSI activity.", publisher = "Serbian Society for Microbiology", journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health", title = "CHEMICAL COMPOSITION AND QUORUM SENSING INHIBITION ACTIVITY OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS", pages = "147-147", url = "https://hdl.handle.net/21.15107/rcub_imagine_2382" }
Stanisavljević, N., Malešević, M., Ćurčić, J., Matijašević, D., Kostić, A., Milinčić, D., Gašić, U.,& Pešić, M.. (2024). CHEMICAL COMPOSITION AND QUORUM SENSING INHIBITION ACTIVITY OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health Serbian Society for Microbiology., 147-147. https://hdl.handle.net/21.15107/rcub_imagine_2382
Stanisavljević N, Malešević M, Ćurčić J, Matijašević D, Kostić A, Milinčić D, Gašić U, Pešić M. CHEMICAL COMPOSITION AND QUORUM SENSING INHIBITION ACTIVITY OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:147-147. https://hdl.handle.net/21.15107/rcub_imagine_2382 .
Stanisavljević, Nemanja, Malešević, Milka, Ćurčić, Jovana, Matijašević, Danka, Kostić, Aleksandar, Milinčić, Danijel, Gašić, Uroš, Pešić, Mirjana, "CHEMICAL COMPOSITION AND QUORUM SENSING INHIBITION ACTIVITY OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):147-147, https://hdl.handle.net/21.15107/rcub_imagine_2382 .