FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA
Autori
Mitrović, HristinaBrdarić, Emilija
Bisenić, Aleksandar
Jakovljević, Stefan
Dinić, Miroslav
Đokić, Jelena
Terzić-Vidojević, Amarela
Tolinački, Maja
Radojević, Dušan
Golić, Nataša
Soković Bajić, Svetlana
Ostala autorstva
Dimkić, IvicaKekić, Dušan
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Psychobiotics are live bacterial strains impacting the central nervous system, producing
neuroactive substances like GABA. GABA from
microbiota influences neural signals, affecting
neurological parameters, sleep, appetite, mood,
and cognition, traversing the intestinal barrier to bind to receptors on enteric neurons and
the vagus nerve. Lactobacillus and Bifidobacterium species can synthesize GABA from dietary
glutamate, with Lactobacillus rhamnosus shown
to reduce anxiety and depressive behavior, elevating hippocampal GABA. Limited knowledge
exists about anaerobic GABA producers, warranting further research for a comprehensive
understanding. Material for isolation comprised
fecal samples from healthy donors, with isolation conducted in an anaerobic chamber within
a maximum of 1 hour after sampling. Isolated
bacteria were identified through sequencing
the 16S rRNA gene. For bacterial cultivation, different types of media were used. PYG medium
contains hematine and vitamin... K, essential supplements for the cultivation of anaerobic bacteria. All media included 0.1% L-cysteine, playing a
role in oxygen reduction, and 0.5% glutamate, a
precursor for GABA production. After identification, the presence of GABA in 8 tested bacterial
species was determined using the TLC method.
Quantification of GABA was performed using the
HPLC method. Furthermore, the positive effects
observed in Caco2 cells with induced inflammation, after treatment with certain anaerobic postbiotics producing GABA, indicate the potential
anti-inflammatory effects of these postbiotics.
The study implies anti-inflammatory effects of
anaerobic GABA producers, offering insights into the complex interplay among gut microbiota,
immune function, and mental health. Recognizing inflammation’s role in depressive symptoms,
targeting anaerobic bacteria involved in GABA
synthesis could modulate neurotransmitters and
inflammatory responses, presenting a comprehensive approach to mental well-being. Advancing research in this area contributes to a holistic
understanding of anaerobic bacteria, GABA production, gut microbiota, and mental health. This
offers avenues for novel therapeutic approaches
and enhances overall quality of life.
Ključne reči:
GUT bacteria / GABA / psychobiotics / anti-inflammatory potentialIzvor:
XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health, 2024, 111-111Izdavač:
- Serbian Society for Microbiology
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200042 (Univerzitet u Beogradu, Institut za molekularnu genetiku i genetičko inženjerstvo) (RS-MESTD-inst-2020-200042)
- NextGenBiotics - The use of integrative multi-omics approach in cultivation and characterization of gut bacteria related to microbiota-gut-brain axis as a source for Next Generation Probiotics (RS-ScienceFundRS-Ideje-7744507)
Napomena:
- Book of abstract: From biotechnology to human and planetary health XIII congress of microbiologists of Serbia with international participation Mikromed regio 5, ums series 24: 4th – 6th april 2024, Mona Plaza hotel, Belgrade, Serbia
Kolekcije
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - CONF AU - Mitrović, Hristina AU - Brdarić, Emilija AU - Bisenić, Aleksandar AU - Jakovljević, Stefan AU - Dinić, Miroslav AU - Đokić, Jelena AU - Terzić-Vidojević, Amarela AU - Tolinački, Maja AU - Radojević, Dušan AU - Golić, Nataša AU - Soković Bajić, Svetlana PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2389 AB - Psychobiotics are live bacterial strains impacting the central nervous system, producing neuroactive substances like GABA. GABA from microbiota influences neural signals, affecting neurological parameters, sleep, appetite, mood, and cognition, traversing the intestinal barrier to bind to receptors on enteric neurons and the vagus nerve. Lactobacillus and Bifidobacterium species can synthesize GABA from dietary glutamate, with Lactobacillus rhamnosus shown to reduce anxiety and depressive behavior, elevating hippocampal GABA. Limited knowledge exists about anaerobic GABA producers, warranting further research for a comprehensive understanding. Material for isolation comprised fecal samples from healthy donors, with isolation conducted in an anaerobic chamber within a maximum of 1 hour after sampling. Isolated bacteria were identified through sequencing the 16S rRNA gene. For bacterial cultivation, different types of media were used. PYG medium contains hematine and vitamin K, essential supplements for the cultivation of anaerobic bacteria. All media included 0.1% L-cysteine, playing a role in oxygen reduction, and 0.5% glutamate, a precursor for GABA production. After identification, the presence of GABA in 8 tested bacterial species was determined using the TLC method. Quantification of GABA was performed using the HPLC method. Furthermore, the positive effects observed in Caco2 cells with induced inflammation, after treatment with certain anaerobic postbiotics producing GABA, indicate the potential anti-inflammatory effects of these postbiotics. The study implies anti-inflammatory effects of anaerobic GABA producers, offering insights into the complex interplay among gut microbiota, immune function, and mental health. Recognizing inflammation’s role in depressive symptoms, targeting anaerobic bacteria involved in GABA synthesis could modulate neurotransmitters and inflammatory responses, presenting a comprehensive approach to mental well-being. Advancing research in this area contributes to a holistic understanding of anaerobic bacteria, GABA production, gut microbiota, and mental health. This offers avenues for novel therapeutic approaches and enhances overall quality of life. PB - Serbian Society for Microbiology C3 - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health T1 - FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA EP - 111 SP - 111 UR - https://hdl.handle.net/21.15107/rcub_imagine_2389 ER -
@conference{ author = "Mitrović, Hristina and Brdarić, Emilija and Bisenić, Aleksandar and Jakovljević, Stefan and Dinić, Miroslav and Đokić, Jelena and Terzić-Vidojević, Amarela and Tolinački, Maja and Radojević, Dušan and Golić, Nataša and Soković Bajić, Svetlana", year = "2024", abstract = "Psychobiotics are live bacterial strains impacting the central nervous system, producing neuroactive substances like GABA. GABA from microbiota influences neural signals, affecting neurological parameters, sleep, appetite, mood, and cognition, traversing the intestinal barrier to bind to receptors on enteric neurons and the vagus nerve. Lactobacillus and Bifidobacterium species can synthesize GABA from dietary glutamate, with Lactobacillus rhamnosus shown to reduce anxiety and depressive behavior, elevating hippocampal GABA. Limited knowledge exists about anaerobic GABA producers, warranting further research for a comprehensive understanding. Material for isolation comprised fecal samples from healthy donors, with isolation conducted in an anaerobic chamber within a maximum of 1 hour after sampling. Isolated bacteria were identified through sequencing the 16S rRNA gene. For bacterial cultivation, different types of media were used. PYG medium contains hematine and vitamin K, essential supplements for the cultivation of anaerobic bacteria. All media included 0.1% L-cysteine, playing a role in oxygen reduction, and 0.5% glutamate, a precursor for GABA production. After identification, the presence of GABA in 8 tested bacterial species was determined using the TLC method. Quantification of GABA was performed using the HPLC method. Furthermore, the positive effects observed in Caco2 cells with induced inflammation, after treatment with certain anaerobic postbiotics producing GABA, indicate the potential anti-inflammatory effects of these postbiotics. The study implies anti-inflammatory effects of anaerobic GABA producers, offering insights into the complex interplay among gut microbiota, immune function, and mental health. Recognizing inflammation’s role in depressive symptoms, targeting anaerobic bacteria involved in GABA synthesis could modulate neurotransmitters and inflammatory responses, presenting a comprehensive approach to mental well-being. Advancing research in this area contributes to a holistic understanding of anaerobic bacteria, GABA production, gut microbiota, and mental health. This offers avenues for novel therapeutic approaches and enhances overall quality of life.", publisher = "Serbian Society for Microbiology", journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health", title = "FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA", pages = "111-111", url = "https://hdl.handle.net/21.15107/rcub_imagine_2389" }
Mitrović, H., Brdarić, E., Bisenić, A., Jakovljević, S., Dinić, M., Đokić, J., Terzić-Vidojević, A., Tolinački, M., Radojević, D., Golić, N.,& Soković Bajić, S.. (2024). FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health Serbian Society for Microbiology., 111-111. https://hdl.handle.net/21.15107/rcub_imagine_2389
Mitrović H, Brdarić E, Bisenić A, Jakovljević S, Dinić M, Đokić J, Terzić-Vidojević A, Tolinački M, Radojević D, Golić N, Soković Bajić S. FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:111-111. https://hdl.handle.net/21.15107/rcub_imagine_2389 .
Mitrović, Hristina, Brdarić, Emilija, Bisenić, Aleksandar, Jakovljević, Stefan, Dinić, Miroslav, Đokić, Jelena, Terzić-Vidojević, Amarela, Tolinački, Maja, Radojević, Dušan, Golić, Nataša, Soković Bajić, Svetlana, "FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):111-111, https://hdl.handle.net/21.15107/rcub_imagine_2389 .