Enzyme stabilization for biodegradation of organophosphate plastic additives and pesticides (orgOPhix)
Apstrakt
Organophosphates (OPs) enjoy wide application in numerous industrial sectors, including plastic
production, agriculture, and the arms industry. These chemicals serve as plastic additives to improve
product properties, as pesticides to protect crops from pests and diseases, and as chemical weapons in
form of nerve agents. The widespread use of OPs has led to the near-ubiquitous accumulation of OPs in
soils and waters across the globe, with profound health and environmental repercussions. Acute exposure
to anthropogenic OPs causes toxicity in insects, plants, animals, and humans, while chronic exposure has
been linked to neurotoxic effects, developmental abnormalities, and increased risk of certain cancers. The
widespread pollution and high toxicity of OPs require development of efficient and eco-friendly
decontamination methods. Currently, only enzymatic degradation of OPs meets these criteria. However,
just a few enzymes involved in OPs degradation have been discovered. Among ...them are two novel
enzymes that we identified, which independently and efficiently degrade a wide range of OP plastic
additives, pesticides, and nerve agents1. Nonetheless, their soluble expression and storage stability was
highly problematic, limiting cost-effective production and broad OP decontamination potential.
Therefore, we plan to improve their soluble expression and environmental stability using protein
engineering tools such as ancestral sequence reconstruction and stability optimization algorithms.
Stabilized enzymes will have а wide range of potential applications, from water and soil remediation to
prophylactic protection against OP poisoning. Finally, we will develop bacteria carrying novel
phosphotriesterases as part of the OPs metabolic pathway. The bacteria will be used for OPs
bioremediation converting these toxic compounds into phosphate, a molecule essential for life.
Izvor:
Science Fund of the Republic of Serbia, Program Proof of Concept, 2024Finansiranje / projekti:
- orgOPhix - Enzyme stabilization for biodegradation of organophosphate plastic additives and pesticides (RS-ScienceFundRS-DokazKoncepta-14551)
Napomena:
- Principal Investigator: Dr Dragana Despotović, IMGGE
- Duration period: 2024-2025
Kolekcije
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - GEN PY - 2024 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/2400 AB - Organophosphates (OPs) enjoy wide application in numerous industrial sectors, including plastic production, agriculture, and the arms industry. These chemicals serve as plastic additives to improve product properties, as pesticides to protect crops from pests and diseases, and as chemical weapons in form of nerve agents. The widespread use of OPs has led to the near-ubiquitous accumulation of OPs in soils and waters across the globe, with profound health and environmental repercussions. Acute exposure to anthropogenic OPs causes toxicity in insects, plants, animals, and humans, while chronic exposure has been linked to neurotoxic effects, developmental abnormalities, and increased risk of certain cancers. The widespread pollution and high toxicity of OPs require development of efficient and eco-friendly decontamination methods. Currently, only enzymatic degradation of OPs meets these criteria. However, just a few enzymes involved in OPs degradation have been discovered. Among them are two novel enzymes that we identified, which independently and efficiently degrade a wide range of OP plastic additives, pesticides, and nerve agents1. Nonetheless, their soluble expression and storage stability was highly problematic, limiting cost-effective production and broad OP decontamination potential. Therefore, we plan to improve their soluble expression and environmental stability using protein engineering tools such as ancestral sequence reconstruction and stability optimization algorithms. Stabilized enzymes will have а wide range of potential applications, from water and soil remediation to prophylactic protection against OP poisoning. Finally, we will develop bacteria carrying novel phosphotriesterases as part of the OPs metabolic pathway. The bacteria will be used for OPs bioremediation converting these toxic compounds into phosphate, a molecule essential for life. T2 - Science Fund of the Republic of Serbia, Program Proof of Concept T1 - Enzyme stabilization for biodegradation of organophosphate plastic additives and pesticides (orgOPhix) UR - https://hdl.handle.net/21.15107/rcub_imagine_2400 ER -
@misc{ year = "2024", abstract = "Organophosphates (OPs) enjoy wide application in numerous industrial sectors, including plastic production, agriculture, and the arms industry. These chemicals serve as plastic additives to improve product properties, as pesticides to protect crops from pests and diseases, and as chemical weapons in form of nerve agents. The widespread use of OPs has led to the near-ubiquitous accumulation of OPs in soils and waters across the globe, with profound health and environmental repercussions. Acute exposure to anthropogenic OPs causes toxicity in insects, plants, animals, and humans, while chronic exposure has been linked to neurotoxic effects, developmental abnormalities, and increased risk of certain cancers. The widespread pollution and high toxicity of OPs require development of efficient and eco-friendly decontamination methods. Currently, only enzymatic degradation of OPs meets these criteria. However, just a few enzymes involved in OPs degradation have been discovered. Among them are two novel enzymes that we identified, which independently and efficiently degrade a wide range of OP plastic additives, pesticides, and nerve agents1. Nonetheless, their soluble expression and storage stability was highly problematic, limiting cost-effective production and broad OP decontamination potential. Therefore, we plan to improve their soluble expression and environmental stability using protein engineering tools such as ancestral sequence reconstruction and stability optimization algorithms. Stabilized enzymes will have а wide range of potential applications, from water and soil remediation to prophylactic protection against OP poisoning. Finally, we will develop bacteria carrying novel phosphotriesterases as part of the OPs metabolic pathway. The bacteria will be used for OPs bioremediation converting these toxic compounds into phosphate, a molecule essential for life.", journal = "Science Fund of the Republic of Serbia, Program Proof of Concept", title = "Enzyme stabilization for biodegradation of organophosphate plastic additives and pesticides (orgOPhix)", url = "https://hdl.handle.net/21.15107/rcub_imagine_2400" }
(2024). Enzyme stabilization for biodegradation of organophosphate plastic additives and pesticides (orgOPhix). in Science Fund of the Republic of Serbia, Program Proof of Concept. https://hdl.handle.net/21.15107/rcub_imagine_2400
Enzyme stabilization for biodegradation of organophosphate plastic additives and pesticides (orgOPhix). in Science Fund of the Republic of Serbia, Program Proof of Concept. 2024;. https://hdl.handle.net/21.15107/rcub_imagine_2400 .
"Enzyme stabilization for biodegradation of organophosphate plastic additives and pesticides (orgOPhix)" in Science Fund of the Republic of Serbia, Program Proof of Concept (2024), https://hdl.handle.net/21.15107/rcub_imagine_2400 .