A novel role for cardiac ankyrin repeat protein Ankrd1/CARP as a co-activator of the p53 tumor suppressor protein
Само за регистроване кориснике
2010
Аутори
Kojić, SnežanaNestorović, Aleksandra
Rakićević, Ljiljana
Belgrano, Anna
Stanković, Marija
Divac Rankov, Aleksandra
Faulkner, Georgine
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The muscle ankyrin repeat protein (MARP) family member Ankrd1/CARP is a part of the titin-mechanosensory signaling complex in the sarcomere and in response to stretch it translocates to the nucleus where it participates in the regulation of cardiac genes as a transcriptional co-repressor. Several studies have focused on its structural role in muscle, but its regulatory role is still poorly understood To gain more insight into the regulatory function of Ankrd1/CARP we searched for transcription factors that could interact and modulate its activity. Using protein array methodology we identified the tumor suppressor protein p53 as an Ankrd1/CARP interacting partner and confirmed their interaction both in vivo and in vitro We demonstrate a novel role for Ankrd1/CARP as a transcriptional co-activator, moderately up regulating p53 activity Furthermore, we show that p53 operates as an upstream effector of Ankrd1/CARP, by up regulating the proximal ANKRD1 promoter Our findings suggest that, be...sides acting as a transcriptional co-repressor, Ankrd1/CARP could have a stimulatory effect on gene expression in cultured skeletal muscle cells It is probable that Ankrd1 /CARP has a role in the propagation of signals initiated by myogenic regulatory factors (MRFs) during myogenesis
Кључне речи:
Transcription factor / Skeletal muscle / p53 / Myogenesis / Ankrd1/CARPИзвор:
Archives of Biochemistry and Biophysics, 2010, 502, 1, 60-67Издавач:
- Elsevier Science Inc, New York
Финансирање / пројекти:
- ICGEB [CRP/YUG-05-01]
- Структурални елементи генома у модулацији фенотипа (RS-MESTD-MPN2006-2010-143051)
- Telethon-Italy to G Faulkner [GGP04088]
DOI: 10.1016/j.abb.2010.06.029
ISSN: 0003-9861
PubMed: 20599664
WoS: 000281718000009
Scopus: 2-s2.0-77956182195
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Snežana AU - Nestorović, Aleksandra AU - Rakićević, Ljiljana AU - Belgrano, Anna AU - Stanković, Marija AU - Divac Rankov, Aleksandra AU - Faulkner, Georgine PY - 2010 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/445 AB - The muscle ankyrin repeat protein (MARP) family member Ankrd1/CARP is a part of the titin-mechanosensory signaling complex in the sarcomere and in response to stretch it translocates to the nucleus where it participates in the regulation of cardiac genes as a transcriptional co-repressor. Several studies have focused on its structural role in muscle, but its regulatory role is still poorly understood To gain more insight into the regulatory function of Ankrd1/CARP we searched for transcription factors that could interact and modulate its activity. Using protein array methodology we identified the tumor suppressor protein p53 as an Ankrd1/CARP interacting partner and confirmed their interaction both in vivo and in vitro We demonstrate a novel role for Ankrd1/CARP as a transcriptional co-activator, moderately up regulating p53 activity Furthermore, we show that p53 operates as an upstream effector of Ankrd1/CARP, by up regulating the proximal ANKRD1 promoter Our findings suggest that, besides acting as a transcriptional co-repressor, Ankrd1/CARP could have a stimulatory effect on gene expression in cultured skeletal muscle cells It is probable that Ankrd1 /CARP has a role in the propagation of signals initiated by myogenic regulatory factors (MRFs) during myogenesis PB - Elsevier Science Inc, New York T2 - Archives of Biochemistry and Biophysics T1 - A novel role for cardiac ankyrin repeat protein Ankrd1/CARP as a co-activator of the p53 tumor suppressor protein EP - 67 IS - 1 SP - 60 VL - 502 DO - 10.1016/j.abb.2010.06.029 ER -
@article{ author = "Kojić, Snežana and Nestorović, Aleksandra and Rakićević, Ljiljana and Belgrano, Anna and Stanković, Marija and Divac Rankov, Aleksandra and Faulkner, Georgine", year = "2010", abstract = "The muscle ankyrin repeat protein (MARP) family member Ankrd1/CARP is a part of the titin-mechanosensory signaling complex in the sarcomere and in response to stretch it translocates to the nucleus where it participates in the regulation of cardiac genes as a transcriptional co-repressor. Several studies have focused on its structural role in muscle, but its regulatory role is still poorly understood To gain more insight into the regulatory function of Ankrd1/CARP we searched for transcription factors that could interact and modulate its activity. Using protein array methodology we identified the tumor suppressor protein p53 as an Ankrd1/CARP interacting partner and confirmed their interaction both in vivo and in vitro We demonstrate a novel role for Ankrd1/CARP as a transcriptional co-activator, moderately up regulating p53 activity Furthermore, we show that p53 operates as an upstream effector of Ankrd1/CARP, by up regulating the proximal ANKRD1 promoter Our findings suggest that, besides acting as a transcriptional co-repressor, Ankrd1/CARP could have a stimulatory effect on gene expression in cultured skeletal muscle cells It is probable that Ankrd1 /CARP has a role in the propagation of signals initiated by myogenic regulatory factors (MRFs) during myogenesis", publisher = "Elsevier Science Inc, New York", journal = "Archives of Biochemistry and Biophysics", title = "A novel role for cardiac ankyrin repeat protein Ankrd1/CARP as a co-activator of the p53 tumor suppressor protein", pages = "67-60", number = "1", volume = "502", doi = "10.1016/j.abb.2010.06.029" }
Kojić, S., Nestorović, A., Rakićević, L., Belgrano, A., Stanković, M., Divac Rankov, A.,& Faulkner, G.. (2010). A novel role for cardiac ankyrin repeat protein Ankrd1/CARP as a co-activator of the p53 tumor suppressor protein. in Archives of Biochemistry and Biophysics Elsevier Science Inc, New York., 502(1), 60-67. https://doi.org/10.1016/j.abb.2010.06.029
Kojić S, Nestorović A, Rakićević L, Belgrano A, Stanković M, Divac Rankov A, Faulkner G. A novel role for cardiac ankyrin repeat protein Ankrd1/CARP as a co-activator of the p53 tumor suppressor protein. in Archives of Biochemistry and Biophysics. 2010;502(1):60-67. doi:10.1016/j.abb.2010.06.029 .
Kojić, Snežana, Nestorović, Aleksandra, Rakićević, Ljiljana, Belgrano, Anna, Stanković, Marija, Divac Rankov, Aleksandra, Faulkner, Georgine, "A novel role for cardiac ankyrin repeat protein Ankrd1/CARP as a co-activator of the p53 tumor suppressor protein" in Archives of Biochemistry and Biophysics, 502, no. 1 (2010):60-67, https://doi.org/10.1016/j.abb.2010.06.029 . .