Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull.
Establishing a model system for transformation of I. hawkerii Bull. plants
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Milošević, SnežanaCingel, Aleksandar
Ninković, Slavica
Nikolić, Dragana
Jevremović, Slađana
Tadić, Vojin
Subotić, Angelina
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Jedna od vodećih oblasti savremene biotehnologije je dobijanje biljaka otpornih na patogene pomoću metoda genetičkog inženjeringa. U ovom radu je proučavana genetička transformacija Impatiens hawkerii Bull. pomoću Agrobacterium tumefaciens C58C1pac1, a cilj je dobijanje biljaka koje nose pac1 gen za rezistentnost na virus bronzavosti paradajza i druge biljne RNK viruse. Transformanati I. hawkerii Bull. na selektivnoj podlozi su brzo propadali, a ni jedan klon nije preživeo peti pasaž. Ovo propadanje je posledica velike osetljivosti ove vrste na kanamicin ili neuspešnosti transfomacije.
One of the leading areas of modern biotechnology is to develop plants resistant to pathogens using genetic engineering methods. The genetic transformation of I. hawkerii Bull. with Agrobacterium tumefaciens C58C1pac1 was observe in this study, and the aim was to derive plants carrying the gene for pac1 resistance to tomato spotted wilt virus and other plant RNA viruses. I. hawkerii Bull. transformants on a selective medium rapidly failed, and not a single clone survived the fifth passage. This decline was due to great susceptibility of this type to kanamycin or failure of the transformation.
Keywords:
kanamicin / I. hawkerii / A. tumefaciens C58C1pac1Source:
Journal of Scientific Agricultural Research, 2011, 72, 1, 23-27Publisher:
- Savez poljoprivrednih inženjera i tehničara, Beograd
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Milošević, Snežana AU - Cingel, Aleksandar AU - Ninković, Slavica AU - Nikolić, Dragana AU - Jevremović, Slađana AU - Tadić, Vojin AU - Subotić, Angelina PY - 2011 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/506 AB - Jedna od vodećih oblasti savremene biotehnologije je dobijanje biljaka otpornih na patogene pomoću metoda genetičkog inženjeringa. U ovom radu je proučavana genetička transformacija Impatiens hawkerii Bull. pomoću Agrobacterium tumefaciens C58C1pac1, a cilj je dobijanje biljaka koje nose pac1 gen za rezistentnost na virus bronzavosti paradajza i druge biljne RNK viruse. Transformanati I. hawkerii Bull. na selektivnoj podlozi su brzo propadali, a ni jedan klon nije preživeo peti pasaž. Ovo propadanje je posledica velike osetljivosti ove vrste na kanamicin ili neuspešnosti transfomacije. AB - One of the leading areas of modern biotechnology is to develop plants resistant to pathogens using genetic engineering methods. The genetic transformation of I. hawkerii Bull. with Agrobacterium tumefaciens C58C1pac1 was observe in this study, and the aim was to derive plants carrying the gene for pac1 resistance to tomato spotted wilt virus and other plant RNA viruses. I. hawkerii Bull. transformants on a selective medium rapidly failed, and not a single clone survived the fifth passage. This decline was due to great susceptibility of this type to kanamycin or failure of the transformation. PB - Savez poljoprivrednih inženjera i tehničara, Beograd T2 - Journal of Scientific Agricultural Research T1 - Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull. T1 - Establishing a model system for transformation of I. hawkerii Bull. plants EP - 27 IS - 1 SP - 23 VL - 72 UR - https://hdl.handle.net/21.15107/rcub_ibiss_366 ER -
@article{ author = "Milošević, Snežana and Cingel, Aleksandar and Ninković, Slavica and Nikolić, Dragana and Jevremović, Slađana and Tadić, Vojin and Subotić, Angelina", year = "2011", abstract = "Jedna od vodećih oblasti savremene biotehnologije je dobijanje biljaka otpornih na patogene pomoću metoda genetičkog inženjeringa. U ovom radu je proučavana genetička transformacija Impatiens hawkerii Bull. pomoću Agrobacterium tumefaciens C58C1pac1, a cilj je dobijanje biljaka koje nose pac1 gen za rezistentnost na virus bronzavosti paradajza i druge biljne RNK viruse. Transformanati I. hawkerii Bull. na selektivnoj podlozi su brzo propadali, a ni jedan klon nije preživeo peti pasaž. Ovo propadanje je posledica velike osetljivosti ove vrste na kanamicin ili neuspešnosti transfomacije., One of the leading areas of modern biotechnology is to develop plants resistant to pathogens using genetic engineering methods. The genetic transformation of I. hawkerii Bull. with Agrobacterium tumefaciens C58C1pac1 was observe in this study, and the aim was to derive plants carrying the gene for pac1 resistance to tomato spotted wilt virus and other plant RNA viruses. I. hawkerii Bull. transformants on a selective medium rapidly failed, and not a single clone survived the fifth passage. This decline was due to great susceptibility of this type to kanamycin or failure of the transformation.", publisher = "Savez poljoprivrednih inženjera i tehničara, Beograd", journal = "Journal of Scientific Agricultural Research", title = "Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull., Establishing a model system for transformation of I. hawkerii Bull. plants", pages = "27-23", number = "1", volume = "72", url = "https://hdl.handle.net/21.15107/rcub_ibiss_366" }
Milošević, S., Cingel, A., Ninković, S., Nikolić, D., Jevremović, S., Tadić, V.,& Subotić, A.. (2011). Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull.. in Journal of Scientific Agricultural Research Savez poljoprivrednih inženjera i tehničara, Beograd., 72(1), 23-27. https://hdl.handle.net/21.15107/rcub_ibiss_366
Milošević S, Cingel A, Ninković S, Nikolić D, Jevremović S, Tadić V, Subotić A. Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull.. in Journal of Scientific Agricultural Research. 2011;72(1):23-27. https://hdl.handle.net/21.15107/rcub_ibiss_366 .
Milošević, Snežana, Cingel, Aleksandar, Ninković, Slavica, Nikolić, Dragana, Jevremović, Slađana, Tadić, Vojin, Subotić, Angelina, "Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull." in Journal of Scientific Agricultural Research, 72, no. 1 (2011):23-27, https://hdl.handle.net/21.15107/rcub_ibiss_366 .