Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk
2013
Authors
Begović, JelenaJovčić, Branko
Papić-Obradović, Milena
Veljović, Katarina
Lukić, Jovanka
Kojić, Milan
Topisirović, Ljubiša
Article (Published version)
Metadata
Show full item recordAbstract
Staphylococcus epidermidis strains were isolated from the expressed human breast milk (EHM) of 14 healthy donor mothers. Genetic diversity was evaluated using RAPD-PCR REP-PCR and pulse-field gel electrophoresis (PFGE). PFGE allowed the best discrimination of the isolates, since it provided for the greatest diversity of the analyzed genomes. Among the S. epidermidis strains, resistance to gentamicin, tetracycline, erythromycin, clindamycin or vancomycin was detected, whilst four isolates were multiresistant. The results from our study demonstrate that staphylococci from EHM could be reservoirs of resistance genes, since we showed that tetK could be transferred from EHM staphylococci to Gram-negative Escherichia coli. Most of the staphylococcal strains displayed excellent proteolytic and lipolytic activities. Additionally, the presence of ica genes, which was related to their ability to form a biofilm on tissue culture plates, and the presence of virulence factors including autolysin/ad...hesin AtLE, point to their pathogenic potential.
Keywords:
Virulence / S. epidermidis / Human breast milk / Antibiotic susceptibilitySource:
Microbiological Research, 2013, 168, 2, 77-83Publisher:
- Elsevier Gmbh, Munich
Funding / projects:
- Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.1016/j.micres.2012.09.004
ISSN: 0944-5013
PubMed: 23098640
WoS: 000315179400003
Scopus: 2-s2.0-84872598109
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Begović, Jelena AU - Jovčić, Branko AU - Papić-Obradović, Milena AU - Veljović, Katarina AU - Lukić, Jovanka AU - Kojić, Milan AU - Topisirović, Ljubiša PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/622 AB - Staphylococcus epidermidis strains were isolated from the expressed human breast milk (EHM) of 14 healthy donor mothers. Genetic diversity was evaluated using RAPD-PCR REP-PCR and pulse-field gel electrophoresis (PFGE). PFGE allowed the best discrimination of the isolates, since it provided for the greatest diversity of the analyzed genomes. Among the S. epidermidis strains, resistance to gentamicin, tetracycline, erythromycin, clindamycin or vancomycin was detected, whilst four isolates were multiresistant. The results from our study demonstrate that staphylococci from EHM could be reservoirs of resistance genes, since we showed that tetK could be transferred from EHM staphylococci to Gram-negative Escherichia coli. Most of the staphylococcal strains displayed excellent proteolytic and lipolytic activities. Additionally, the presence of ica genes, which was related to their ability to form a biofilm on tissue culture plates, and the presence of virulence factors including autolysin/adhesin AtLE, point to their pathogenic potential. PB - Elsevier Gmbh, Munich T2 - Microbiological Research T1 - Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk EP - 83 IS - 2 SP - 77 VL - 168 DO - 10.1016/j.micres.2012.09.004 ER -
@article{ author = "Begović, Jelena and Jovčić, Branko and Papić-Obradović, Milena and Veljović, Katarina and Lukić, Jovanka and Kojić, Milan and Topisirović, Ljubiša", year = "2013", abstract = "Staphylococcus epidermidis strains were isolated from the expressed human breast milk (EHM) of 14 healthy donor mothers. Genetic diversity was evaluated using RAPD-PCR REP-PCR and pulse-field gel electrophoresis (PFGE). PFGE allowed the best discrimination of the isolates, since it provided for the greatest diversity of the analyzed genomes. Among the S. epidermidis strains, resistance to gentamicin, tetracycline, erythromycin, clindamycin or vancomycin was detected, whilst four isolates were multiresistant. The results from our study demonstrate that staphylococci from EHM could be reservoirs of resistance genes, since we showed that tetK could be transferred from EHM staphylococci to Gram-negative Escherichia coli. Most of the staphylococcal strains displayed excellent proteolytic and lipolytic activities. Additionally, the presence of ica genes, which was related to their ability to form a biofilm on tissue culture plates, and the presence of virulence factors including autolysin/adhesin AtLE, point to their pathogenic potential.", publisher = "Elsevier Gmbh, Munich", journal = "Microbiological Research", title = "Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk", pages = "83-77", number = "2", volume = "168", doi = "10.1016/j.micres.2012.09.004" }
Begović, J., Jovčić, B., Papić-Obradović, M., Veljović, K., Lukić, J., Kojić, M.,& Topisirović, L.. (2013). Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk. in Microbiological Research Elsevier Gmbh, Munich., 168(2), 77-83. https://doi.org/10.1016/j.micres.2012.09.004
Begović J, Jovčić B, Papić-Obradović M, Veljović K, Lukić J, Kojić M, Topisirović L. Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk. in Microbiological Research. 2013;168(2):77-83. doi:10.1016/j.micres.2012.09.004 .
Begović, Jelena, Jovčić, Branko, Papić-Obradović, Milena, Veljović, Katarina, Lukić, Jovanka, Kojić, Milan, Topisirović, Ljubiša, "Genotypic diversity and virulent factors of Staphylococcus epidermidis isolated from human breast milk" in Microbiological Research, 168, no. 2 (2013):77-83, https://doi.org/10.1016/j.micres.2012.09.004 . .