Monoclonal antibody 26 cross-reactive with beta(2)-glycoprotein I affects human trophoblast invasion in vitro
Abstract
Objective: Monoclonal antibody 26 (MAb 26) raised against tetanus toxoid has documented cross-reactivity with beta(2)-glycoprotein I. Passive introduction of this antibody in mice results in an antiphospholipid syndrome-like condition. We investigated the effects of MAb 26 on first trimester human trophoblast in vitro. Study design: Binding of MAb 26 to placental tissue trophoblast, isolated cytotrophoblast and HTR-8/SVneo cells was analyzed by immunohisto(cyto)chemistry. Possible effects on cell invasion in vitro were assessed by Matrigel assay. Effects on cell viability were assessed by MU test. A possibility that MAb 26 induces change in levels of effector molecules important for cell invasion was investigated. Integrin subunits alpha(1), alpha(5) and beta(1), and galectin-1, were analyzed by qPCR and Western blot. Metalloproteinases -2 and -9 were assessed by gelatin zymography. Results: Immunohisto(cyto)chemistry showed binding of MAb 26 to placental tissue trophoblast, isolated c...ytotrophoblast and HTR-8/SVneo cells. The antibody had a significant inhibitory effect on cell invasion by both isolated cytotrophoblast and HTR-8/SVneo. The antibody induced significant decrease in protein levels of metalloproteinases, integrin subunit alpha(1) and galectin-1. Cell viability was not affected. Conclusion: MAb 26 reduces trophoblast invasion in vitro through decreased levels of metalloproteinases-2 and -9, integrin alpha(1) and galectin-1.
Keywords:
Trophoblast invasion / Anti-tetanus toxoid / Antiphospholipid syndrome / Anti-beta(2)-glycoprotein ISource:
European Journal of Obstetrics & Gynecology and Reproductive Biology, 2013, 171, 1, 23-29Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Trophoblast and extraembryonic fetal cells: plasticity, differentiation factors and in vitro modulation of functional characteristics (RS-MESTD-Basic Research (BR or ON)-173004)
DOI: 10.1016/j.ejogrb.2013.08.005
ISSN: 0301-2115
PubMed: 24012450
WoS: 000327562900006
Scopus: 2-s2.0-84887085296
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kovacević, Tamara M. AU - Radojcić, Ljiljana AU - Tošić, Nataša AU - Pavlović, Sonja AU - Vicovac, Ljiljana M. PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/702 AB - Objective: Monoclonal antibody 26 (MAb 26) raised against tetanus toxoid has documented cross-reactivity with beta(2)-glycoprotein I. Passive introduction of this antibody in mice results in an antiphospholipid syndrome-like condition. We investigated the effects of MAb 26 on first trimester human trophoblast in vitro. Study design: Binding of MAb 26 to placental tissue trophoblast, isolated cytotrophoblast and HTR-8/SVneo cells was analyzed by immunohisto(cyto)chemistry. Possible effects on cell invasion in vitro were assessed by Matrigel assay. Effects on cell viability were assessed by MU test. A possibility that MAb 26 induces change in levels of effector molecules important for cell invasion was investigated. Integrin subunits alpha(1), alpha(5) and beta(1), and galectin-1, were analyzed by qPCR and Western blot. Metalloproteinases -2 and -9 were assessed by gelatin zymography. Results: Immunohisto(cyto)chemistry showed binding of MAb 26 to placental tissue trophoblast, isolated cytotrophoblast and HTR-8/SVneo cells. The antibody had a significant inhibitory effect on cell invasion by both isolated cytotrophoblast and HTR-8/SVneo. The antibody induced significant decrease in protein levels of metalloproteinases, integrin subunit alpha(1) and galectin-1. Cell viability was not affected. Conclusion: MAb 26 reduces trophoblast invasion in vitro through decreased levels of metalloproteinases-2 and -9, integrin alpha(1) and galectin-1. PB - Elsevier, Amsterdam T2 - European Journal of Obstetrics & Gynecology and Reproductive Biology T1 - Monoclonal antibody 26 cross-reactive with beta(2)-glycoprotein I affects human trophoblast invasion in vitro EP - 29 IS - 1 SP - 23 VL - 171 DO - 10.1016/j.ejogrb.2013.08.005 ER -
@article{ author = "Kovacević, Tamara M. and Radojcić, Ljiljana and Tošić, Nataša and Pavlović, Sonja and Vicovac, Ljiljana M.", year = "2013", abstract = "Objective: Monoclonal antibody 26 (MAb 26) raised against tetanus toxoid has documented cross-reactivity with beta(2)-glycoprotein I. Passive introduction of this antibody in mice results in an antiphospholipid syndrome-like condition. We investigated the effects of MAb 26 on first trimester human trophoblast in vitro. Study design: Binding of MAb 26 to placental tissue trophoblast, isolated cytotrophoblast and HTR-8/SVneo cells was analyzed by immunohisto(cyto)chemistry. Possible effects on cell invasion in vitro were assessed by Matrigel assay. Effects on cell viability were assessed by MU test. A possibility that MAb 26 induces change in levels of effector molecules important for cell invasion was investigated. Integrin subunits alpha(1), alpha(5) and beta(1), and galectin-1, were analyzed by qPCR and Western blot. Metalloproteinases -2 and -9 were assessed by gelatin zymography. Results: Immunohisto(cyto)chemistry showed binding of MAb 26 to placental tissue trophoblast, isolated cytotrophoblast and HTR-8/SVneo cells. The antibody had a significant inhibitory effect on cell invasion by both isolated cytotrophoblast and HTR-8/SVneo. The antibody induced significant decrease in protein levels of metalloproteinases, integrin subunit alpha(1) and galectin-1. Cell viability was not affected. Conclusion: MAb 26 reduces trophoblast invasion in vitro through decreased levels of metalloproteinases-2 and -9, integrin alpha(1) and galectin-1.", publisher = "Elsevier, Amsterdam", journal = "European Journal of Obstetrics & Gynecology and Reproductive Biology", title = "Monoclonal antibody 26 cross-reactive with beta(2)-glycoprotein I affects human trophoblast invasion in vitro", pages = "29-23", number = "1", volume = "171", doi = "10.1016/j.ejogrb.2013.08.005" }
Kovacević, T. M., Radojcić, L., Tošić, N., Pavlović, S.,& Vicovac, L. M.. (2013). Monoclonal antibody 26 cross-reactive with beta(2)-glycoprotein I affects human trophoblast invasion in vitro. in European Journal of Obstetrics & Gynecology and Reproductive Biology Elsevier, Amsterdam., 171(1), 23-29. https://doi.org/10.1016/j.ejogrb.2013.08.005
Kovacević TM, Radojcić L, Tošić N, Pavlović S, Vicovac LM. Monoclonal antibody 26 cross-reactive with beta(2)-glycoprotein I affects human trophoblast invasion in vitro. in European Journal of Obstetrics & Gynecology and Reproductive Biology. 2013;171(1):23-29. doi:10.1016/j.ejogrb.2013.08.005 .
Kovacević, Tamara M., Radojcić, Ljiljana, Tošić, Nataša, Pavlović, Sonja, Vicovac, Ljiljana M., "Monoclonal antibody 26 cross-reactive with beta(2)-glycoprotein I affects human trophoblast invasion in vitro" in European Journal of Obstetrics & Gynecology and Reproductive Biology, 171, no. 1 (2013):23-29, https://doi.org/10.1016/j.ejogrb.2013.08.005 . .