Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia
Abstract
New Delhi metallo-beta-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla (NDM-1) gene. Restriction analysis and hybridization revealed that two copies of the bla (NDM-1) gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla (NDM-1) gene in the genome of P. aeruginosa MMA83.
Keywords:
Pseudomonas aeruginosa / NDM-1 / Molecular genetics / Metallo-beta-lactamaseSource:
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2014, 105, 3, 613-618Publisher:
- Springer, Dordrecht
Funding / projects:
- Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.1007/s10482-013-0094-z
ISSN: 0003-6072
PubMed: 24343100
WoS: 000330953700018
Scopus: 2-s2.0-84894254640
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jovčić, Branko AU - Lepsanović, Zorica AU - Begović, Jelena AU - Filipić, Brankica AU - Kojić, Milan PY - 2014 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/757 AB - New Delhi metallo-beta-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla (NDM-1) gene. Restriction analysis and hybridization revealed that two copies of the bla (NDM-1) gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla (NDM-1) gene in the genome of P. aeruginosa MMA83. PB - Springer, Dordrecht T2 - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology T1 - Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia EP - 618 IS - 3 SP - 613 VL - 105 DO - 10.1007/s10482-013-0094-z ER -
@article{ author = "Jovčić, Branko and Lepsanović, Zorica and Begović, Jelena and Filipić, Brankica and Kojić, Milan", year = "2014", abstract = "New Delhi metallo-beta-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla (NDM-1) gene. Restriction analysis and hybridization revealed that two copies of the bla (NDM-1) gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla (NDM-1) gene in the genome of P. aeruginosa MMA83.", publisher = "Springer, Dordrecht", journal = "Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology", title = "Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia", pages = "618-613", number = "3", volume = "105", doi = "10.1007/s10482-013-0094-z" }
Jovčić, B., Lepsanović, Z., Begović, J., Filipić, B.,& Kojić, M.. (2014). Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Springer, Dordrecht., 105(3), 613-618. https://doi.org/10.1007/s10482-013-0094-z
Jovčić B, Lepsanović Z, Begović J, Filipić B, Kojić M. Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2014;105(3):613-618. doi:10.1007/s10482-013-0094-z .
Jovčić, Branko, Lepsanović, Zorica, Begović, Jelena, Filipić, Brankica, Kojić, Milan, "Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia" in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 105, no. 3 (2014):613-618, https://doi.org/10.1007/s10482-013-0094-z . .