Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity
2015
Authors
Stanisavljević, NemanjaIlić, Marija
Jovanović, Živko
Cupić, Tihomir
Dabić, Dragana C.
Natić, Maja M.
Tesić, Zivoslav Lj.
Radović, Svetlana S.
Article (Published version)
Metadata
Show full item recordAbstract
The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antiox...idant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.
Keywords:
UHPLC-LTQ OrbiTrap MS / seed coat / Pisum sativum L. / phenolic content / antioxidantSource:
Archives of Biological Sciences, 2015, 67, 3, 829-840Publisher:
- Srpsko biološko društvo, Beograd, i dr.
Funding / projects:
- The Role of Transcription Factors and Small RNAs in Abiotic Stress Response in Plants and Genetic Diversity of Plant Species Important for Agriculture and Biotechnology (RS-MESTD-Basic Research (BR or ON)-173005)
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-MESTD-Basic Research (BR or ON)-172017)
DOI: 10.2298/ABS141204042S
ISSN: 0354-4664
WoS: 000366794800008
Scopus: 2-s2.0-84950117705
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stanisavljević, Nemanja AU - Ilić, Marija AU - Jovanović, Živko AU - Cupić, Tihomir AU - Dabić, Dragana C. AU - Natić, Maja M. AU - Tesić, Zivoslav Lj. AU - Radović, Svetlana S. PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/832 AB - The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity EP - 840 IS - 3 SP - 829 VL - 67 DO - 10.2298/ABS141204042S ER -
@article{ author = "Stanisavljević, Nemanja and Ilić, Marija and Jovanović, Živko and Cupić, Tihomir and Dabić, Dragana C. and Natić, Maja M. and Tesić, Zivoslav Lj. and Radović, Svetlana S.", year = "2015", abstract = "The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity", pages = "840-829", number = "3", volume = "67", doi = "10.2298/ABS141204042S" }
Stanisavljević, N., Ilić, M., Jovanović, Ž., Cupić, T., Dabić, D. C., Natić, M. M., Tesić, Z. Lj.,& Radović, S. S.. (2015). Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 67(3), 829-840. https://doi.org/10.2298/ABS141204042S
Stanisavljević N, Ilić M, Jovanović Ž, Cupić T, Dabić DC, Natić MM, Tesić ZL, Radović SS. Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity. in Archives of Biological Sciences. 2015;67(3):829-840. doi:10.2298/ABS141204042S .
Stanisavljević, Nemanja, Ilić, Marija, Jovanović, Živko, Cupić, Tihomir, Dabić, Dragana C., Natić, Maja M., Tesić, Zivoslav Lj., Radović, Svetlana S., "Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity" in Archives of Biological Sciences, 67, no. 3 (2015):829-840, https://doi.org/10.2298/ABS141204042S . .